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OSMI-4 Transferase inhibitor

Name: OSMI-4
Brand: Selleck Chemicals
SKU: S8910
Availability: InStock
Rating: 5 (7 reviews)

Cat.No.S8910

OSMI-4 is an inhibitor of O-linked N-acetylglucosamine transferase (O-GlcNAc transferase, OGT) with EC50 of 3 μM in cells.

Chemical Structure

Molecular Weight: 604.09

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S891001 DMSO]100 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.93%

99.93

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Chemical Information, Storage & Stability

Molecular Weight	604.09	Formula	C₂₇H₂₆ClN₃O₇S₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2260791-14-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CCOC(=O)CN(CC1=CC=CS1)C(=O)C(C2=CC=CC=C2OC)NS(=O)(=O)C3=C(C=C4C(=C3)C=CC(=O)N4)Cl

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (165.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5% DMSO 1 20% PEG300 2 3%Tween80 3 72%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (8.28mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 200 μL of PEG300, mix evenly to clarify it; add 30 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 720 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	OGT ^[1] (Cell-free assay) 3 μM(EC50)
In vitro	OSMI-4 reduces O-GlcNAc levels almost completely by 5 μM. A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 is observed in cells treated with this compound. This chemical is a kind of structure-based evolution of small molecule OGT inhibitors with low nanomolar binding affinities. It exhibits a ~3 μM EC50 in cells, making it especially attractive for probing OGT’s complex biology.^[1]
References	[1] https://pubmed.ncbi.nlm.nih.gov/30285435/

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