Home Metabolism Transferase inhibitor OSMI-4 For research use only.

OSMI-4

OSMI-4 is an inhibitor of O-linked N-acetylglucosamine transferase (O-GlcNAc transferase, OGT) with EC50 of 3 μM in cells.

OSMI-4 Chemical Structure

OSMI-4 Chemical Structure

CAS: 2260791-14-6

Selleck's OSMI-4 has been cited by 4 publications

Purity & Quality Control

Batch: S891001 DMSO] 100 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.93%
99.93

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Biological Activity

Description OSMI-4 is an inhibitor of O-linked N-acetylglucosamine transferase (O-GlcNAc transferase, OGT) with EC50 of 3 μM in cells.
Targets
OGT [1]
(Cell-free assay)
3 μM(EC50)
In vitro
In vitro

OSMI-4 reduces O-GlcNAc levels almost completely by 5 μM. A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 is observed in cells treated with OSMI-4. OSMI-4 is a kind of structure-based evolution of small molecule OGT inhibitors with low nanomolar binding affinities. OSMI-4 exhibits a ~3 μM EC50 in cells, making it especially attractive for probing OGT’s complex biology.[1]
Cell Research Cell lines HEK293T cells
Concentrations 5 μM, 10 μM, 20 μM
Incubation Time 2 h, 8 h, 16 h, 24 h
Method

For quantitative analysis of the proteome, HEK293T cells in fresh media (changed 3 hours before treatment) are treated with 5 and 20 μM OSMI-4 in triplicates, and 10 μM in duplicates (11-plex) or DMSO for 8 hours, 16 hours or 24 hours. At the indicated time point, cells are lysed in lysis buffer (2 % SDS 50 mM Tris-HCl and 150 mM NaCl), sonicated and protein concentration determined with the BCA assay.

Chemical Information & Solubility

Molecular Weight 604.09 Formula

C27H26ClN3O7S2
CAS No. 2260791-14-6 SDF --
Smiles CCOC(=O)CN(CC1=CC=CS1)C(=O)C(C2=CC=CC=C2OC)NS(=O)(=O)C3=C(C=C4C(=C3)C=CC(=O)N4)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (165.53 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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