SGN-2FF

Catalog No.S9954 Synonyms: 2-fluorofucose

For research use only.

SGN‐2FF (2-fluorofucose) is an orally bioavailable small‐molecule inhibitor of fucosyltransferase that demonstrated encouraging preclinical antitumor activity in mouse models.

SGN-2FF Chemical Structure

CAS No. 2089647-47-0

Purity & Quality Control

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Biological Activity

Description

SGN‐2FF (2-fluorofucose) is an orally bioavailable small‐molecule inhibitor of fucosyltransferase that demonstrated encouraging preclinical antitumor activity in mouse models.

Targets
fucosyltransferase [1]
()
In vitro

SGN‐2FF can fully inhibit the fucosylation of the h1F6 mAb at the lowest screening concentration in CHO cells treated with SGN‐2FF. The functional effect of inhibiting fucosylation of LS174T cells with SGN‐2FF is apparent from its diminished adherence to E-selectin–coated plates.[2]

In vivo

In mice, SGN‐2FF inhibits fucosylation of endogenously produced antibodies, tumor xenograft membranes, and neutrophil adhesion glycans. SGN‐2FF treatment affords complete protection from tumor engraftment in a syngeneic tumor vaccine model, inhibits neutrophil extravasation, and delays the outgrowth of tumor xenografts in immune-deficient mice. [2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: human LS174T colorectal cells, CHO cells
  • Concentrations: 100 μM
  • Incubation Time: 10 days
  • Method:

    LS174T cells are cultured in Eagle MEM with 10% FBS with or without 100 μM SGN‐2FF for 10 d. LS174T xenografts from SGN‐2FF-treated or untreated mice are disaggregated by treatment with collagenase, and loose cells are collected. Cells from either source are washed with PBS solution containing 2% FBS and 0.02% sodium azide and stained with biotinylated LCA, cBR96-Alexa Fluor 488, or SSEAI–Alexa Fluor 488 (1 h on ice). Cells are washed and resuspended in PBS solution/FBS/sodium azide at 4 °C. The lectin-stained cells are treated with FITC-conjugated avidin D (30 min on ice), washed, and then analyzes by flow cytometry.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 33 mg/mL
(198.61 mM)
Water 33 mg/mL
(198.61 mM)
Ethanol 8 mg/mL
(48.14 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 166.15
Formula

C6H11FO4

CAS No. 2089647-47-0
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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