Name: Propylthiouracil
Brand: Selleck Chemicals
SKU: S1988
Availability: InStock
Rating: 5 (2 reviews)

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Quality Control

Batch: S198801 DMSO]80 mg/mL]false]Ethanol]19 mg/mL]false]Water]Insoluble]false Purity: 99.46%

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COA
NMR
HPLC
SDS
Datasheet

99.46

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Peroxidases Inhibitors	ML-210 4-Aminobenzohydrazide Verdiperstat PF-06282999 JKE-1674 ZX703 FINO2 Alkyne tyramide MPO-IN-28 4-Methylesculetin

Solubility

In vitro
Batch:

DMSO : 80 mg/mL (469.95 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 19 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.700mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.700mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	170.23	Formula	C₇H₁₀N₂OS	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	51-52-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC 6498, NSC 70461			Smiles	CCCC1=CC(=O)NC(=S)N1

Mechanism of Action

Targets/IC₅₀/Ki	thyroperoxidase 5'-deiodinase
In vivo	Propylthiouracil-induced hypothyroidism reduces oxidative damage in the lung, hepatic, renal and ileal tissues probably due to hypometabolism in rats, which is associated with decreased production of reactive oxygen metabolites and enhancement of antioxidant mechanisms. This compound-induced congenital hypothyroidism upregulates vimentin phosphorylation and depletes antioxidant defenses in immature rat testis. It results in lesser concentrations of thyroxine (T4) and triiodothyronine (T3), greater concentrations of follicle stimulating hormone (FSH) and luteinizing hormone (LH) peptides, and increase in steroidogenic gene expression after 12 hours and 48 hours in zebrafish. This compound-induced hypothyroidism is associated with increased tolerance of the isolated rat heart toischaemia-reperfusion. It dramatically reduces thyroid hormones on PND21 and produced deficits in body weight that persisted to adulthood in developing rats. This compound inhibits both the synthesis of thyroid hormones in the thyroid gland and the conversion of T4 to its active form, T3. This treatment significantly increases circulating TSH at both P3 and P7. This exposure of adult rats, at a dose inducing modest reductions in circulating T4 concentrations and no significant effect on brain BDNF, significantly alteres the thyroid hormones and hippocampal BDNF levels in the offspring at 3 and 7 d after birth.
References	[1] https://pubmed.ncbi.nlm.nih.gov/16648293/ [2] https://pubmed.ncbi.nlm.nih.gov/18316471/ [3] https://pubmed.ncbi.nlm.nih.gov/21158436/ [4] https://pubmed.ncbi.nlm.nih.gov/12967335/ [5] https://pubmed.ncbi.nlm.nih.gov/14519490/ [6] https://pubmed.ncbi.nlm.nih.gov/22253429/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02390180	Completed	Taste Qualities\|Primary Tastes	Purdue University	October 2014	--
NCT01097915	Completed	Dysgeusia	University of Cagliari	October 2009	--
NCT00570466	Completed	Type 2 Diabetes	National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)\|Baylor College of Medicine	January 2008	Not Applicable

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Propylthiouracil Peroxidases inhibitor

Chemical Structure

Molecular Weight: 170.23