4-Aminobenzohydrazide

Catalog No.S9874 Synonyms: 4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640

For research use only.

4-Aminobenzohydrazide (4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640) is an inhibitor of MPO (Myeloperoxidase) enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.

4-Aminobenzohydrazide Chemical Structure

CAS No. 5351-17-7

Purity & Quality Control

Choose Selective Peroxidases Inhibitors

Biological Activity

Description 4-Aminobenzohydrazide (4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640) is an inhibitor of MPO (Myeloperoxidase) enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.
Targets
MPO [1]
()
In vitro

4-aminobenzohydrazide is an inhibitor of MPO enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.[1]

In vivo

Hamsters treated with ABAH show no significant differences in the percentage of lesions or in the percentage of amoebae damaged compared with the untreated hamsters. ABAH treated mice versus untreated mice show larger abscesses and a decreased percentage of damaged amoebae in these lesion at all stages of evolution.[2]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: bone marrow cells
  • Concentrations: 0.1 μM, 10 μM, 100 μM, 200 μM
  • Incubation Time: 8 h
  • Method:

    If MPO suppresses the induction of iNOS by IL, inhibitors of MPO enzyme activity should alleviate this suppression. To test this, bone marrow cells from TgM-POg and MPO-KO mice are incubated with IFNc/LPS for 8 h in the presence of increasing concentrations of 4-aminobenzohydrazide (ABAH).

  • (Only for Reference)
Animal Research:

[2]

  • Animal Models: 9 week-old Hamsters, 7–10 week-old male BALB/c mice
  • Dosages: 40 mg/kg
  • Administration: IP
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 30 mg/mL
(198.45 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 151.17
Formula

C7H9N3O

CAS No. 5351-17-7
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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