research use only

4-Aminobenzohydrazide Peroxidases inhibitor

Name: 4-Aminobenzohydrazide
Brand: Selleck Chemicals
SKU: S9874
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S9874

4-Aminobenzohydrazide (4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640) is an inhibitor of MPO (Myeloperoxidase) enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.

Chemical Structure

Molecular Weight: 151.17

Jump to

Quality Control

Batch: S987401 DMSO]30 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.96%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.96

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Peroxidases Inhibitors	ML-210 Verdiperstat PF-06282999 JKE-1674 ZX703 FINO2 Alkyne tyramide MPO-IN-28 4-Methylesculetin PKUMDL-LC-101-D04

Solubility

In vitro
Batch:

DMSO : 30 mg/mL (198.45 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (33.08mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	151.17	Formula	C₇H₉N₃O	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	5351-17-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	4-Aminobenzhydrazide, 4-aminobenzoic acid hydrazide, 4-ABAH, Myeloperoxidase Inhibitor I, NSC 640			Smiles	NNC(=O)C1=CC=C(N)C=C1

Mechanism of Action

Targets/IC₅₀/Ki	MPO
In vitro	4-aminobenzohydrazide is an inhibitor of MPO enzyme that enhances iNOS induction in MPO-positive cells, but not in MPO-KO cells.
In vivo	Hamsters treated with ABAH show no significant differences in the percentage of lesions or in the percentage of amoebae damaged compared with the untreated hamsters. ABAH treated mice versus untreated mice show larger abscesses and a decreased percentage of damaged amoebae in these lesion at all stages of evolution.
References	[1] https://pubmed.ncbi.nlm.nih.gov/15893945/ [2] https://pubmed.ncbi.nlm.nih.gov/28796788/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):