Donepezil HCl

For research use only.

Catalog No.S2462

5 publications

Donepezil HCl  Chemical Structure

CAS No. 120011-70-3

Donepezil is a specific and potent AChE inhibitor for bAChE and hAChE with IC50 of 8.12 nM and 11.6 nM , respectively.

Selleck's Donepezil HCl has been cited by 5 publications

2 Customer Reviews

  • Donepezil-mediated eACh activity in vitro. After being cultured in serum-free EBM-2, HUVECs were treated with donepezil at a concentration of 10 umol/L for 24 hours. E and F: Representative images (x 400 magnification) of anti-ChAT and -VAChT immunofluorescence. Scale bar: 50 um. ChAT indicates choline acetyltransferase; eACh, endothelium-derived acetylcholine; EBM-2, endothelial basal medium 2; HUVECs, human umbilical vein endothelial cells; PCR, polymerase chain reaction; VAChT, vesicular acetylcholine transporter.

    J Am Heart Assoc 2014 3(3), e000804. Donepezil HCl purchased from Selleck.

    Inhibition studies of known inhibitors against BACE1. a Dose-response curves for compounds inhibition of BACE1 in the MMSA (microfluidics-based mobility shift assay).

    Anal Bioanal Chem, 2017, 409(28):6635-6642. Donepezil HCl purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Donepezil is a specific and potent AChE inhibitor for bAChE and hAChE with IC50 of 8.12 nM and 11.6 nM , respectively.
bAChE [1] hAChE [1]
8.12 nM 11.6 nM
In vitro

Donepezil inhibits the carbachol-stimulated increase in intracellular Ca2+ concentration in human SHSY5Y neuroblastoma cells in a concentration dependent manner, indicating that Donepezil have muscarinic antagonist activity. [2] A recent study shows that Donepezil can protect human umbilical vein endothelial cells (HUVECs) against H2O2-induced cell injury. This may be useful as a potential therapy for oxidative stress in cardiovascular and cerebrovascular diseases. [3]

In vivo Intraperitoneal administration of Donepezil in rats produces a dose dependent increase in salivation and tremor, which are overt cholinergic behavioural signs, with an ED50 of 6 μmol/kg. Donepezil is found to be somewhat less potent with a ED50 of 50 μmol/kg following oral administration. [2]. When administered separately in vivo, 5-HT(4) receptor inducer, RS67333 (0.3 and 1 mg/kg) and Donepezil (1 mg/kg) improves recognition performances compared to saline treated mice, while co-administration of subactive doses of RS67333 (0.1mg/kg) and Donepezil (0.3 mg/kg) improves memory. However, this improvement is prevented if a 5-HT(4)R antagonist (GR125487, 10 mg/kg) is also administered. [4]


Animal Research:[2]
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  • Animal Models: Male Lister Hooded rats
  • Dosages: 18 μM/kg
  • Administration: Administered via i.p. or p.o.
    (Only for Reference)

Solubility (25°C)

In vitro Water 5 mg/mL (12.01 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% propylene glycol, 5% Tween 80, 65% D5W
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 416


CAS No. 120011-70-3
Storage powder
in solvent
Synonyms N/A
Smiles COC1=C(C=C2C(=C1)CC(C2=O)CC3CCN(CC3)CC4=CC=CC=C4)OC.Cl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04308304 Recruiting Drug: MK-1942|Drug: Donepezil|Drug: Placebo Alzheimer''s Disease Merck Sharp & Dohme Corp. February 16 2021 Phase 1
NCT04617782 Completed Combination Product: Donepezil TDS|Drug: Aricept Healthy Subjects Corium Inc. December 8 2020 Phase 1
NCT03905096 Completed Drug: BI 425809|Drug: Donepezil Healthy Boehringer Ingelheim April 12 2019 Phase 1
NCT03438604 Completed Drug: Donepezil TDS Alzheimer''s Disease Corium Inc. February 13 2018 Phase 1

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AChR Signaling Pathway Map

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