Donepezil HCl

Catalog No.S2462

Donepezil HCl  Chemical Structure

Molecular Weight(MW): 416

Donepezil is a specific and potent AChE inhibitor for bAChE and hAChE with IC50 of 8.12 nM and 11.6 nM , respectively.

Size Price Stock Quantity  
USD 107 In stock
USD 170 In stock
USD 347 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 4 Publications

2 Customer Reviews

  • Donepezil-mediated eACh activity in vitro. After being cultured in serum-free EBM-2, HUVECs were treated with donepezil at a concentration of 10 umol/L for 24 hours. E and F: Representative images (x 400 magnification) of anti-ChAT and -VAChT immunofluorescence. Scale bar: 50 um. ChAT indicates choline acetyltransferase; eACh, endothelium-derived acetylcholine; EBM-2, endothelial basal medium 2; HUVECs, human umbilical vein endothelial cells; PCR, polymerase chain reaction; VAChT, vesicular acetylcholine transporter.

    J Am Heart Assoc 2014 3(3), e000804. Donepezil HCl purchased from Selleck.

    Inhibition studies of known inhibitors against BACE1. a Dose-response curves for compounds inhibition of BACE1 in the MMSA (microfluidics-based mobility shift assay).

    Anal Bioanal Chem, 2017, 409(28):6635-6642. Donepezil HCl purchased from Selleck.

Purity & Quality Control

Choose Selective AChR Inhibitors

Biological Activity

Description Donepezil is a specific and potent AChE inhibitor for bAChE and hAChE with IC50 of 8.12 nM and 11.6 nM , respectively.
Targets
bAChE [1] hAChE [1]
8.12 nM 11.6 nM
In vitro

Donepezil inhibits the carbachol-stimulated increase in intracellular Ca2+ concentration in human SHSY5Y neuroblastoma cells in a concentration dependent manner, indicating that Donepezil have muscarinic antagonist activity. [2] A recent study shows that Donepezil can protect human umbilical vein endothelial cells (HUVECs) against H2O2-induced cell injury. This may be useful as a potential therapy for oxidative stress in cardiovascular and cerebrovascular diseases. [3]

In vivo Intraperitoneal administration of Donepezil in rats produces a dose dependent increase in salivation and tremor, which are overt cholinergic behavioural signs, with an ED50 of 6 μmol/kg. Donepezil is found to be somewhat less potent with a ED50 of 50 μmol/kg following oral administration. [2]. When administered separately in vivo, 5-HT(4) receptor inducer, RS67333 (0.3 and 1 mg/kg) and Donepezil (1 mg/kg) improves recognition performances compared to saline treated mice, while co-administration of subactive doses of RS67333 (0.1mg/kg) and Donepezil (0.3 mg/kg) improves memory. However, this improvement is prevented if a 5-HT(4)R antagonist (GR125487, 10 mg/kg) is also administered. [4]

Protocol

Animal Research:[2]
+ Expand
  • Animal Models: Male Lister Hooded rats
  • Formulation: 0.9% w:v NaCl (saline)
  • Dosages: 18 μM/kg
  • Administration: Administered via i.p. or p.o.
    (Only for Reference)

Solubility (25°C)

In vitro Water 5 mg/mL (12.01 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% propylene glycol, 5% Tween 80, 65% D5W
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 416
Formula

C24H29NO3.HCl

CAS No. 120011-70-3
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03932916 Not yet recruiting Drug: Donepezil pamoate AD Shanghai Synergy Pharmaceutical Sciences Co. Ltd. May 20 2019 Phase 1
NCT03438604 Completed Drug: Donepezil TDS Alzheimer''s Disease Corium International Inc. February 13 2018 Phase 1
NCT03432195 Completed Drug: Donepezil TDS Alzheimer''s Disease Corium International Inc. January 31 2018 Phase 1
NCT03397862 Completed Drug: Donepezil TDS|Drug: Vehicle TDS Skin Irritation|Sensitization Corium International Inc. November 14 2017 Phase 1
NCT02860065 Withdrawn Drug: CPC-201|Other: Positron emission tomography (PET) Alzheimer''s Disease Chase Pharmaceuticals Corporation an affiliate of Allergan plc September 30 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

AChR Signaling Pathway Map

Related AChR Products

Tags: buy Donepezil HCl | Donepezil HCl supplier | purchase Donepezil HCl | Donepezil HCl cost | Donepezil HCl manufacturer | order Donepezil HCl | Donepezil HCl distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID