(-)-Epigallocatechin Gallate

For research use only.

Catalog No.S2250 Synonyms: EGCG

25 publications

(-)-Epigallocatechin Gallate Chemical Structure

CAS No. 989-51-5

(-)-Epigallocatechin Gallate(EGCG) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.

Selleck's (-)-Epigallocatechin Gallate has been cited by 25 publications

Purity & Quality Control

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Biological Activity

Description (-)-Epigallocatechin Gallate(EGCG) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.
Targets
telomerase [4]
()
DNMT [4]
()
HER2 [5]
()
EGFR [5]
()
FASN [6]
()
In vitro

(-)-Epigallocatechin gallate functions as a powerful antioxidant, preventing oxidative damage in healthy cells, but also as an antiangiogenic and antitumor agent and as a modulator of tumor cell response to chemotherapy. (-)-Epigallocatechin gallate shows multiple anticancer effects, such as anti-proliferation, anti-angiogenesis, transformation prevention of various cancer cells, cancer cell cycle arrest and inhibition of tumor metastasis. (-)-Epigallocatechin gallate exerts multi-anticancer effects through regulating several cancer-related cell signal pathways (regulated the function or the expression of key signal proteins, such as nuclear factor-κB, MAPKs and activator protein-1, EGFR, IGF, COX-2 signaling pathway, and so on.), effecting methylation of cancer genes and combination of ligand with membrane receptors. [1] (-)-Epigallocatechin gallate also shows an immunomodulating effects. Several types of immune cells in both the innate and adaptive immune systems are known to be affected in varying degrees by (-)-Epigallocatechin gallate. Among them, the dramatic effect on T cell functions has been repeatedly demonstrated, including T cell activation, proliferation, differentiation, and production of cytokines. Studies using animal models of autoimmune diseases have reported disease improvement in animals treated with green tea/EGCG. [2] (-)-Epigallocatechin gallate displays anti-infective properties. Antiviral activities of (-)-Epigallocatechin gallate with different modes of action have been demonstrated on diverse families of viruses, such as Retroviridae, Orthomyxoviridae and Flaviviridae and include important human pathogens like human immunodeficiency virus, influenza A virus and the hepatitis C virus. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SH-SY5Y cells MWHGeY5kfGmxbjDhd5NigQ>? M3ruT25mfXKxcILveIVkfGmxbjDh[4FqdnO2IHLleIEu[W27bH;p[EBx\XC2aXTlJFEuPDJvaX7keYNm\CC2b4jpZ4l1gSCrbjDoeY1idiCVSD3TXVV[KGOnbHzzJIF{e2W|c3XkJIF{KGyjY4TheIUh\GWqeXTyc4dmdmG|ZTDy[Yxm[XOnLDDFR|UxRTBwMEO5PFch|ryP NF;D[JAyQTF|OEi1PS=>
human MDA435/LCC6MDR cells MnXlSpVv[3Srb36gZZN{[Xl? MmTqNVAh|ryP M{XYTlUh\GG7cx?= MlvNUY9lfWyjdHnvckBw\iCSLXfwJEh2dmuwb4fuJI9zcWerbjmgeJJidnOoZXP0[YQhcW5iaIXtZY4hVUSDNEO1M2xESz[PRGKgZ4VtdHNiYYPz[ZN{\WRiYYOgdoV3\XK|aXLs[UBw\iCyYXPsbZRigGWuIILld4l{fGGwY3WgcYVie3W{ZXSgZZMhUUN3MDDmc5IheGGlbHn0ZZhmdCCjdDCxNEB2VSCjZoTldkA2KGSjeYOgZpkhS2WubGTpeIVzKDl4IFHxeYVwfXNiYYPzZZktKEmFNUC9NE4yOjJ4IN88US=> MojTNlU6QDVzOUW=
Sf9 cells MX7GeY5kfGmxbjDhd5NigQ>? NIfEPWlKdmirYnn0bY9vKG:oIFjpd|YufGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGRPVVRzIHX4dJJme3OnZDDpckBqdnOnY4SgV4Y6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDEUmEhdWW2aInseJJidnOoZYLhd4Uh[WO2aY\peJkhfXOrbnegOUcu[mmxdHnufYxifGWmIES1MYJxKHWwbXX0bJlt[XSnZDDvdkBp\W2rbXX0bJlt[XSnZDDvcIlod263Y3zlc5Rq\GVic4Xid5Rz[XSnczDhcoQhYzOKXT3B[I9O\XRiYomgcIlyfWmmIIPjbY51cWyuYYTpc44h[2:3boTpcochdWW2aH;kMEBKSzVyPUCuOUDPxE1? M2DyWVI2PDB4OUS0
human U937 cells NX\hbFdJTnWwY4Tpc44h[XO|YYm= MUHJcohq[mm2aX;uJI9nKHSnbH;t[ZJie2ViaX6gbJVu[W5iVUmzO{Bk\WyuczygTWM2OD1zIN88US=> Mn6yNlI1OTN6NEW=
human HeLa cells M4nXT2Z2dmO2aX;uJIF{e2G7 MlnyTY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIFjlUIEh[2WubIOgeZNqdmdiNTetRWFVKEOFRzDUR2chSUeFIFHHRUBIXFRvMzegZZMhe3Wkc4TyZZRmKGmwY4XiZZRm\CCob4KgNVUhdWmwczDwdolweiC2bzDlfJRmdnOrb36gdoVi[3Srb36g[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBjgSC|cHnuMZRmdG:vZYLpZ{Bz\XCnYYSgZY1xdGmoaXPheIlwdiCycn;0c4NwdCxiSVO1NF0yNjB6IN88US=> MWSyNlQyOzh2NR?=
CHO cells NXPxXpk6S3m2b4TvfIlkyqCjc4PhfS=> Mkm1OFghcA>? NFfGNGdEgXSxdH;4bYNqfHliYXfhbY5{fCCFSF:gZ4VtdHNiZYjwdoV{e2mwZzDPRXRROUJ|IHjhdIxwfHmyZTCxJIFnfGW{IES4JIhzeyCkeTDmcJVwemW|Y3XuZ4Uh[mG|ZXSgR4VtdFSrdHXyMWdtdyCjc4PhfUwhUUN3ME2zMlIh|ryP Ml64NlM{Ojd6N{e=
MDCK cells NH3XToZHfW6ldHnvckBie3OjeR?= MV[0JIRigXN? NHq5U2dCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBqdm[udXXufoEhSSC4aYL1d{ApSS:|d3nu[U9QUC93MUG0OFUwOjByNzjINW4yMSliT3i3JIlv\mWldHXkJIlvKE2GQ1ugZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBz\XCuaXPheIlwdiCjZoTldkA1KGSjeYOgZpkheXWjboTpeIF1cX[nIGLUMXBEWixiRVS1NF05NjNizszN NELaZ3kzOjFzNUW5NS=>
human HL60 cells MljiVJJwdGmoZYLheIlwdiCjc4PhfS=> MWCzJIRigXN? NELUcm5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubIOgZYZ1\XJiMzDkZZl{NCCLQ{WwQVkvPCEQvF2= MlG2NVg3QTNyMkC=
HSC-T6 cells  NVTBeJRkTnWwY4Tpc44h[XO|YYm= MVW0PEBp MVLBcpRq\mmkcn;0bYMh[WO2aY\peJkh[WejaX7zeEBz[XRiSGPDMXQ3KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdJJwdGmoZYLheIlwdiCjZoTldkA1QCCqcoOgZpkhSnKmVTDpcoNwenCxcnH0bY9vKGG|c3H5MEBKSzVyPUmuPUDPxE1? MVKyNVUxPDh2OB?=
mouse 3T3-L1 cells MWHGeY5kfGmxbjDhd5NigQ>? MULJcohq[mm2aX;uJI9nKEd4UFStcYVlcWG2ZXSgUmFFWEhicILv[JVkfGmxbjDpckBud3W|ZTCzWFMuVDFiY3XscJMtKEmFNUC9NlUh|ryP NVLpdJpZOTh|MUOzNFg>
mouse RAW264.7 cells NUCxb5Y6TnWwY4Tpc44h[XO|YYm= NXzhVGZxPSCmYYnz NXTKb4lZUW6qaXLpeIlwdiCxZjDSRW5MVC2rbnT1Z4VlKG:|dHXvZ4xie3SxZ3Xu[ZNqeyCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDUVmFRNXCxc3n0bZZmKG23bITpMY52[2ynYYTl[EBk\WyuczDh[pRmeiB3IHThfZMtKEmFNUC9NlkvQCEQvF2= MlmyNlE1PTZ3MkG=
HSC-T6 cells MUXQdo9tcW[ncnH0bY9vKGG|c3H5 M17MbWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgdoF1KEiVQz3UOkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJktKEmFNUC9NlkvQCEQvF2= Ml2yNlU{OjJ2NUW=
human A431 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 NG\4cVM1QCCq NVTINFdLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFMyKGOnbHzzJI93\XKneIDy[ZN{cW6pIFXyZmIhcW5ic3XyeY0u\nKnZTDt[YRqfW1iYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KFeVVD2xJIF{e2G7LDDFR|UxRTN6IN88US=> MVOyOFQ2PjByNB?=
human MDA-MB-231 cells M3HsOHBzd2yrZnXyZZRqd25iYYPzZZk> NHfjdIIzPCCq M4iwb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6 M2r2UFIzPDV7MkC4
human HepG2 cells MXPGeY5kfGmxbjDhd5NigQ>? M1TJNFI1KGh? MnnqTY5pcWKrdHnvckBw\iCxbHXpZ{Bi[2mmLXnu[JVk\WRidILp[4x6[2W{aXTlJI93\XJvYXPjeY12dGG2aX;uJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGmwY4XiZZRm\CCob4KgNlQhcHK|IILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;s MYKyNVgzPDZ7MB?=
human HepG2 cells MmO4SpVv[3Srb36gZZN{[Xl? M4TkZ|I1KGh? MVjBcpRqd3irZHHueEBi[3Srdnn0fUBqdiCqdX3hckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4Yhd2ynaXOgZYNq\C2rbnT1Z4VlKFKRUzDn[Y5memG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IFTIR2YuTEFiYnHz[YQh\my3b4LpcYV1emmlIHHzd4F6KHKnbHH0bZZmKHSxIIXueJJm[XSnZDDjc451em:u M2\tdFIyQDJ2Nkmw
human Caco-2 cells M1Sz[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4W1WFI2KM7:TR?= M3\L[VYh\GG7cx?= M{LMdGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGNi[29vMjDj[YxteyCjdDCyOUB2VSCjZoTldkA3KGSjeYO= MoLtNVg{OjR5NkO=
mouse 3T3 cells M2H3c2Z2dmO2aX;uJIF{e2G7 MWGxMVIxKM7:TR?= NHzkcYMyOiCq MmrmRY51cW2rZ4LheI9zgSCjY4Tpeol1gSCjZ3HpcpN1KFO5aYPzJIFt[mmwbzDtc5V{\SB|VEOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4Yh[2WubDDueY1j\XK|IHH0JFEhfG9iMkCgeW0h[W[2ZYKgNVIhcHK|IHL5JIJ6KHOlcnH0Z4guf2:3bnSgZZN{[Xl? Mnf3NlExQDB4NEK=
human MDA-MB-231 cells M1\GWnBzd2yrZnXyZZRqd25iYYPzZZk> M1nK[FI1KGh? M4OxOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6 NG\UNm4zOjR3OUKwPC=>
human Jurkat cells MUPGeY5kfGmxbjDhd5NigQ>? NGHJZYgyOC13NTFOwG0> NIXqfYIzPCCq MkO3TY5pcWKrdHnvckBw\iCWTl\hcJBp[S2rbnT1Z4VlKE6ILXvhdJBiSiCjY4TpeoF1cW:wIHnuJIh2dWGwIFr1dotifCClZXzsd{BifCBzMDD0c{A2PSC3TTDh[pRmeiB{NDDodpMh[nliZXzlZ5Rzd3Cqb4LleIlkKG2xYnnsbZR6KHOqaX\0JIF{e2G7 MXOyNVA5ODZ2Mh?=
human K562 cells NIrlOppHfW6ldHnvckBie3OjeR?= NW\TdZRKOjRiaB?= M1n2fGlv\HWldHnvckBw\iB4NzDrSIEhdGGvaX7pckBz\WOncITvdkBmgHC{ZYPzbY9vKGmwIHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KG[ub4egZ5l1d22ndIL5JIFv[Wy7c3nz NVznWoFEOjF2M{S2NFM>
human HL60 cells NGDSdGFHfW6ldHnvckBie3OjeR?= NILW[5kzPCCq MUjJcoR2[3Srb36gc4YhPjdia1ThJIxidWmwaX6gdoVk\XC2b4Kg[ZhxemW|c3nvckBqdiCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFI1KGi{czDifUBndG:5IHP5eI9u\XS{eTDhcoFtgXOrcx?= NV;TdHdEOjF2M{S2NFM>
human Raji cells NFPDRYFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV2xNEDPxE1? NXrTSlZHPDhiaB?= NFLMcItIem:5dHigbY5pcWKrdHnvckBi\2GrboP0JIh2dWGwIGLhbokh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IITyfZBidiCkbIXlJIJie2WmIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzJIlvKHC{ZYPlcoNmKG:oIIP1dIVzd3irZHWg[Il{dXW2YYPl NH;PU2IzOTR|NE[wNy=>
human PC3 cells NIfMO|lRem:uaX\ldoF1cW:wIHHzd4F6 MUixNE0yODBizszN MV20PEBp M1\hWWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOzJINmdGy|IHH0JFExKHSxIEGwNEB2VSCjZoTldkA1QCCqcoOgZpkhcGWvb3P5eI9u\XS{aXOgZ4VtdCClb4XueIlv\yCvZYToc4Q> NID6OZQzOzh4N{G2Oy=>
human A431 cells MoH2SpVv[3Srb36gZZN{[Xl? MlHmNVAxKM7:TR?= MXOxNkBp MU\S[YR2[3Srb36gc4Yh[2y3c4Tldolv\yCxZjDHSnAuT1CLIHnuJIxqeGmmIILh[pR{KG:oIHj1cYFvKEF2M{GgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDF{IHjyd{BjgSClb37mc4NidCCvaXPyc5Nkd3CrYzDhcoFtgXOrcx?= NIHpSpIzPDR3NkCwOC=>
human SKBR3 cells NH7KVIpHfW6ldHnvckBie3OjeR?= NFvnZXEzODEQvF2= MkXCN|AhdWmwcx?= NEjuWo9Fd3ewcnXneYxifGmxbjDv[kBGemKEMjDwdo91\WmwIHX4dJJme3Orb36gbY4hcHWvYX6gV2tDWjNiY3XscJMhcW5ic3XyeY0h\nKnZTDt[YRqfW1iYYSgNlAxKHWPIHHmeIVzKDNyIH3pcpMh[nliaX3teY5w\my3b4Lld4NmdmOnIIP0ZYlvcW6pLXLhd4VlKGOxbn\vZ4FtKG2rY4Lvd4NweGmlIHHuZYx6e2m| MkXVNlQ1PTZyMES=
human 293T cells NVPv[HJVTnWwY4Tpc44h[XO|YYm= NH\MZlg2OCEQvF2= M2K5PVEzKGh? MofETY5pcWKrdHnvckBw\iCGWWLLNWEhcW5iaIXtZY4hOjl|VDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhT0yLMTD0doFve2O{aYD0bY9vKGGldHn2bZR6KGG2IEWwJJVOKGGodHXyJFEzKGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MUOyNlE2PDZ4NB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Notch1 / Notch2 ; 

PubMed: 28443100     


Human primary macrophages derived from peripheral blood mononuclear cells (PBMCs) cultured with autologous serum were pretreated with EGCG (50 μg/mL) for 30 min and exposed to LPS (200 EU/mL) for 3 h. Cell lysates from time course experiments of EGCG treatment were analyzed by western blotting and probed with a Notch1/2 antibody  

p-MAPK / MAPK ; 

PubMed: 30233784     


Effects of EGCG on IGF-I-induced phosphorylation of (A) p44/p42 MAP kinase in MC3T3-E1 cells. The cells were pretreated with various doses of EGCG for 60 min, and then stimulated by 10 nM IGF-I or vehicle for (A) 5 min. Western blot analysis was performed using antibodies against phospho-p44/p42 MAP kinase, p44/p42 MAP kinase. The histograms show quantitative representation of the levels of IGF-I-induced phosphorylation determined from laser densitometric analysis of three independent experiments. EGCG, (−)-epigallocatechin gallate; IGF-I, insulin-like growth factor-I; MAP, mitogen-activated protein.

β-catenin / p-AKT / Cyclin D1; 

PubMed: 28693189     


EGCG inhibits β-catenin, p-Akt and cyclin D1 expression in MDA-MB-231 cells. MDA-MB-231 cells were treated with EGCG for 24 h. (A) β-catenin protein expression was analyzed by western blotting. (B) p-Akt and cyclin D1 protein expression were analyzed by western blotting. β-actin was used as a loading control. Each value represents the mean ± standard error of the mean of three independent experiments. EGCG, epigallocatechin gallate; p-, phosphorylated.

28443100 30233784 28693189
Growth inhibiton assay
Cell viability; 

PubMed: 28693189     


Effect of EGCG on the viability of MDA-MB-231 cells. MDA-MB-231 cells were treated with various concentrations of EGCG for 24 h. MTT assay was used to detect the viability of the cells. The optical density value of the untreated cells was regarded as 100%. Each value represents the mean ± standard error of the mean of three independent experiments. EGCG, epigallocatechin gallate.

Cell proliferation ; 

PubMed: 23525843     


The percent EGCG that inhibits the proliferation of colorectal cancer cells at different concentrations. Notes: The x-axis and y-axis are the time after treatment and percentage of EGCG that inhibits the proliferation of colorectal cancer cells at different concentrations. Eight experiments were performed for each treatment, and the inhibition rate of cell proliferation was tested for, using a single factor analysis of variance. Abbreviation: EGCG, epigallocatechin gallate; h, hour.

28693189 23525843

Protocol

Solubility (25°C)

In vitro Ethanol 83 mg/mL warmed (181.07 mM)
DMSO 72 mg/mL warmed (157.07 mM)
Water 23 mg/mL warmed (50.17 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 458.37
Formula

C22H18O11

CAS No. 989-51-5
Storage powder
in solvent
Synonyms EGCG
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03928847 Recruiting Drug: Epigallocatechin-3-gallate (EGCG) Idiopathic Pulmonary Fibrosis University of California San Francisco|National Heart Lung and Blood Institute (NHLBI) July 1 2018 Early Phase 1
NCT02779361 Completed Dietary Supplement: Green tea Healthy Volunteers|Doping in Sports Parc de Salut Mar|World Anti-doping Agency January 2016 Phase 1
NCT02577393 Enrolling by invitation Drug: EGCG|Drug: mLDG Lung Neoplasms|Esophagitis|Prevention & Control|Epigallocatechin Gallate Shandong Cancer Hospital and Institute April 2015 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Telomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID