Home DNA Damage/DNA Repair Telomerase inhibitor - DNA Methyltransferase inhibitor - HER2 inhibitor - EGFR inhibitor - Fatty Acid Synthase inhibitor EGCG ((-)-Epigallocatechin Gallate) For research use only.

EGCG ((-)-Epigallocatechin Gallate)

EGCG ((-)-Epigallocatechin Gallate) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.

EGCG ((-)-Epigallocatechin Gallate) Chemical Structure

EGCG ((-)-Epigallocatechin Gallate) Chemical Structure

CAS: 989-51-5

Selleck's EGCG ((-)-Epigallocatechin Gallate) has been cited by 37 publications

Purity & Quality Control

Batch: Purity: 99.99%
99.99

EGCG ((-)-Epigallocatechin Gallate) Related Products

Signaling Pathway

Telomerase Signaling Pathways

Telomerase Signaling Pathway

Choose Selective Telomerase Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SH-SY5Y cells Function assay Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.03987 μM 19138859
human MDA435/LCC6MDR cells Function assay 10 μM 5 days Modulation of P-gp (unknown origin) transfected in human MDA435/LCC6MDR cells assessed as reversible of paclitaxel resistance measured as IC50 for paclitaxel at 10 uM after 5 days by CellTiter 96 Aqueous assay, IC50=0.1226 μM 25985195
Sf9 cells Function assay Inhibition of His6-tagged human recombinant DNMT1 expressed in insect Sf9 cells assessed as reduction in DNA methyltransferase activity using 5'-biotinylated 45-bp unmethylated or hemimethylated oligonucleotide substrates and [3H]-AdoMet by liquid scintillation counting method, IC50=0.5 μM 25406944
human U937 cells Function assay Inhibition of telomerase in human U937 cells, IC50=1 μM 22413845
human HeLa cells Function assay Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol, IC50=1.08 μM 22413845
CHO cells Cytotoxic assay 48 h Cytotoxicity against CHO cells expressing OATP1B3 haplotype 1 after 48 hrs by fluorescence based CellTiter-Glo assay, IC50=3.2 μM 23327877
MDCK cells Function assay 4 days Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR, ED50=8.3 μM 22115591
human HL60 cells Proliferation assay 3 days Antiproliferative activity against human HL60 cells after 3 days, IC50=9.4 μM 18693020
HSC-T6 cells  Function assay 48 h Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay, IC50=9.9 μM 21504848
mouse 3T3-L1 cells Function assay Inhibition of G6PD-mediated NADPH production in mouse 3T3-L1 cells, IC50=25 μM 18313308
mouse RAW264.7 cells Function assay 5 days Inhibition of RANKL-induced osteoclastogenesis in mouse RAW264.7 cells assessed as decrease in TRAP-positive multi-nucleated cells after 5 days, IC50=29.8 μM 21456521
HSC-T6 cells Proliferation assay Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability, IC50=29.8 μM 25322455
human A431 cells Proliferation assay 48 h Antiproliferative activity against human A431 cells overexpressing ErbB in serum-free medium assessed as cell viability after 48 hrs by WST-1 assay, EC50=38 μM 24456004
human MDA-MB-231 cells Proliferation assay 24 h Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay 22459208
human HepG2 cells Function assay 24 h Inhibition of oleic acid-induced triglyceride over-accumulation in human HepG2 cells incubated for 24 hrs relative to untreated control 21824690
human HepG2 cells Function assay 24 h Antioxidant activity in human HepG2 cells assessed as reduction of oleic acid-induced ROS generation incubated for 24 hrs by DHCF-DA based fluorimetric assay relative to untreated control 21824690
human Caco-2 cells Growth inhibition assay 25 μM 6 days Growth inhibition of human Caco-2 cells at 25 uM after 6 days 18324763
mouse 3T3 cells Function assay 1-20 μM 12 h Antimigratory activity against Swiss albino mouse 3T3 cells assessed as increase of cell numbers at 1 to 20 uM after 12 hrs by by scratch-wound assay 21080642
human MDA-MB-231 cells Proliferation assay 24 h Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay 22459208
human Jurkat cells Function assay 10-55 μM 24 h Inhibition of TNFalpha-induced NF-kappaB activation in human Jurkat cells at 10 to 55 uM after 24 hrs by electrophoretic mobility shift assay 21080642
human K562 cells Function assay 24 h Induction of 67 kDa laminin receptor expression in human K562 cells after 24 hrs by flow cytometry analysis 21434603
human HL60 cells Function assay 24 h Induction of 67 kDa laminin receptor expression in human HL60 cells after 24 hrs by flow cytometry analysis 21434603
human Raji cells Growth inhibition assay 10 μM 48 h Growth inhibition against human Raji cells assessed as cell viability at 10 uM after 48 hrs by trypan blue based microscopic analysis in presence of superoxide dismutase 21434603
human PC3 cells Proliferation assay 10-100 μM 48 h Antiproliferative activity against human PC3 cells at 10 to 100 uM after 48 hrs by hemocytometric cell counting method 23867167
human A431 cells Function assay 100 μM 12 h Reduction of clustering of GFP-GPI in lipid rafts of human A431 cells at 100 uM after 12 hrs by confocal microscopic analysis 24456004
human SKBR3 cells Function assay 200μM 30 mins Downregulation of ErbB2 protein expression in human SKBR3 cells in serum free medium at 200 uM after 30 mins by immunofluorescence staining-based confocal microscopic analysis 24456004
human 293T cells Function assay 50 μM 12 h Inhibition of DYRK1A in human 293T cells assessed as reduction of GLI1 transcription activity at 50 uM after 12 hrs by dual-luciferase reporter gene assay 22154664
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Biological Activity

Description EGCG ((-)-Epigallocatechin Gallate) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.
Targets
telomerase [4] DNMT [4] HER2 [5] EGFR [5] FASN [6]
In vitro
In vitro

(-)-Epigallocatechin gallate functions as a powerful antioxidant, preventing oxidative damage in healthy cells, but also as an antiangiogenic and antitumor agent and as a modulator of tumor cell response to chemotherapy. (-)-Epigallocatechin gallate shows multiple anticancer effects, such as anti-proliferation, anti-angiogenesis, transformation prevention of various cancer cells, cancer cell cycle arrest and inhibition of tumor metastasis. (-)-Epigallocatechin gallate exerts multi-anticancer effects through regulating several cancer-related cell signal pathways (regulated the function or the expression of key signal proteins, such as nuclear factor-κB, MAPKs and activator protein-1, EGFR, IGF, COX-2 signaling pathway, and so on.), effecting methylation of cancer genes and combination of ligand with membrane receptors. [1] (-)-Epigallocatechin gallate also shows an immunomodulating effects. Several types of immune cells in both the innate and adaptive immune systems are known to be affected in varying degrees by (-)-Epigallocatechin gallate. Among them, the dramatic effect on T cell functions has been repeatedly demonstrated, including T cell activation, proliferation, differentiation, and production of cytokines. Studies using animal models of autoimmune diseases have reported disease improvement in animals treated with green tea/EGCG. [2] (-)-Epigallocatechin gallate displays anti-infective properties. Antiviral activities of (-)-Epigallocatechin gallate with different modes of action have been demonstrated on diverse families of viruses, such as Retroviridae, Orthomyxoviridae and Flaviviridae and include important human pathogens like human immunodeficiency virus, influenza A virus and the hepatitis C virus. [3]
Experimental Result Images Methods Biomarkers Images PMID
Western blot β-catenin / p-AKT / Cyclin D1 p-MAPK / MAPK Notch1 / Notch2 28693189
Growth inhibiton assay Cell proliferation Cell viability 23525843
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05758571 Recruiting
Interstitial Pneumonia|Neoplasms Malignant
Shandong Cancer Hospital and Institute
 January 5 2023 Phase 1|Phase 2
NCT03928847 Completed
Idiopathic Pulmonary Fibrosis
Hal Chapman|National Heart Lung and Blood Institute (NHLBI)|University of California San Francisco
 December 1 2017 Early Phase 1

Chemical Information & Solubility

Molecular Weight 458.37 Formula

C22H18O11
CAS No. 989-51-5 SDF Download EGCG ((-)-Epigallocatechin Gallate) SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 91 mg/mL ( (198.52 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 91 mg/mL

Ethanol : 91 mg/mL


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