Name: Cycloastragenol
Brand: Selleck Chemicals
SKU: S3894
Rating: 5 (1 reviews)

Cycloastragenol (CAG, TA-65, Cyclogalegigenin, Astramembrangenin) is a saponin comprising a group of oil glucosides naturally present in a number of plants. It is a potent telomerase activator in neuronal cells.

Cycloastragenol Chemical Structure

CAS: 84605-18-5

Selleck's Cycloastragenol has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 100.00%

100.00

Cycloastragenol Related Products

Signaling Pathway

Telomerase Signaling Pathway

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Biological Activity

Description

Cycloastragenol (CAG, TA-65, Cyclogalegigenin, Astramembrangenin) is a saponin comprising a group of oil glucosides naturally present in a number of plants. It is a potent telomerase activator in neuronal cells.

Targets

telomerase ^[1]

In vitro
In vitro	Cycloastragenol stimulates telomerase activity and cell proliferation in human neonatal keratinocytes. It induces telomerase activity and cAMP response element binding (CREB) activation in PC12 cells and primary neurons. CAG treatment not only induces the expression of bcl2, a CREB-regulated gene, but also the expression of telomerase reverse transcriptase in primary cortical neurons^[1]. Cycloastragenol rapidly passes through the Caco-2 cell monolayer by passive diffusion, once passage through the intestinal epithelium, first-pass intestinal metabolism of Cycloastragenol might occur. Cycloastragenol can undergo extensive metabolism in rat and human liver microsomes^[2].
Cell Research	Cell lines	HEKn cells, PC12 cells
	Concentrations	--
	Incubation Time	24 h
	Method	HEKn cells (population doubling time: 3-6 days), PC12 cells (passage No.: 12-18), cultured primary cortical, and hippocampal neurons [12 days in vitro (DIV)] are treated with CAG for 24 h. Total cell lysates are then collected by using the lysis buffer provided in the real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) assay kit. Telomerase activity is determined by using an ABI Prism 7000.

In Vivo
In vivo	Oral administration of cycloastragenol (CA) for 7 days attenuates depression-like behavior in experimental mice. Oral bioavailability of cycloastragenol is about 25.70% at 10 mg/kg. Cycloastragenol is excreted through bile and feces and eliminated predominantly by the kidney in rats. It also might exist an enterohepatic circulation of cycloastragenol in rats. Cycloastragenol could be metabolized widely in vivo in rat. For oral administration the mean T_max at 10 mg/kg, 20 mg/kg, and 40 mg/kg is 2.06±0.58 h, 1.48±0.36 h, and 2.35± 1.17 h. The t_1/2 is 5.23±1.55 h, 7.33±3.03 h, and 6.06± 3.42 h, respectively. The mean absorption time of CA at 10 mg/kg is 5.70± 1.62 h; the poor absorption could be caused by the low solubility. The pharmacokinetic parameters show no significant differences among the groups of 10, 20, and 40 mg/kg except for C_max and AUC^[2].
Animal Research	Animal Models	male ICR mice
	Dosages	100 mg/kg
	Administration	by oral gavage

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05777083	Recruiting	ST Elevation Myocardial Infarction\|Electrocardiogram	Ewha Womans University Mokdong Hospital	January 1 2023	Not Applicable
NCT05578443	Recruiting	Alzheimer''s Disease	Fujian Medical University Union Hospital	October 1 2022	Phase 2
NCT05528744	Recruiting	Genetic Disease\|Chopra-Amiel-Gordon Syndrome\|CAGS\|ANKRD17	Boston Children''s Hospital	August 27 2022	--
NCT05533528	Recruiting	Periodontal Pocket\|Periodontal Diseases\|Periodontal Bone Loss	Universidad de Murcia	May 3 2022	Not Applicable
NCT05118009	Completed	Myocardial Infarction Acute	National Defense Medical Center Taiwan	May 1 2022	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	490.72	Formula	C₃₀H₅₀O₅
CAS No.	84605-18-5	SDF	--
Smiles	CC1(C(CCC23C1C(CC4C2(C3)CCC5(C4(CC(C5C6(CCC(O6)C(C)(C)O)C)O)C)C)O)O)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 98 mg/mL ( (199.7 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble

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In vivo
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