BIBR 1532

For research use only.

Catalog No.S1186

17 publications

BIBR 1532 Chemical Structure

CAS No. 321674-73-1

BIBR 1532 is a potent, selective, non-competitive telomerase inhibitor with IC50 of 100 nM in a cell-free assay. No inhibition of DNA and RNA polymerases, including HIV reverse transcriptase are observed at concentrations vastly exceeding the IC50 for telomerase. BIBR 1532 induces apoptosis in cancer cells.

Selleck's BIBR 1532 has been cited by 17 publications

2 Customer Reviews

  • J Mol Neurosci 2013 51(1), 187-98. BIBR 1532 purchased from Selleck.

  • (E, F)The cells were not treated or treated with BIBR1532 before wounds were made. Relative ratio of wound closure per field was shown. (G, H) KrasG12D and KrasG12V-induced migration ability of lung cancer cells was inhibited by BIBR1532 in Transwell invasion assay. Numbers of invasive cells in 10 fields were counted. Magnification: ×200. Representative pictures were shown. Values were the mean of 3 determinations ± SEM (one-way ANOVA, *p < 0.05, **p < 0.01).

    Oncotarget, 2017, 8(1):179-190. BIBR 1532 purchased from Selleck.

Purity & Quality Control

Choose Selective Telomerase Inhibitors

Biological Activity

Description BIBR 1532 is a potent, selective, non-competitive telomerase inhibitor with IC50 of 100 nM in a cell-free assay. No inhibition of DNA and RNA polymerases, including HIV reverse transcriptase are observed at concentrations vastly exceeding the IC50 for telomerase. BIBR 1532 induces apoptosis in cancer cells.
Telomerase [1]
(Cell-free assay)
100 nM
In vitro

BIBR 1532 exhibits an non-competitive inhibitory effect on telomerase activity. [1] In JVM13 leukemia cell line, BIBR 1532 shows an antiproliferative effect in a dose-dependent range with IC50 of 52 μM, and similar results are also observed in other leukemia cell lines including Nalm-1, HL-60, and Jurkat. In addition, BIBR 1532 results in a direct antiproliferative effect on acute myeloid leukemia (AML) with IC50 of 56 μM without affecting the proliferative capacity of normal hematopoietic progenitor cells. [2] BIBR 1532 (2.5 μM) reduces colony-forming ability, and induces telomere length shortening as well as chemotherapeutic sensitization by inhibiting telomerase activity in MCF-7/WT and melphalan-resistant MCF-7/MlnR cell lines. [3] In T-cell prolymphocytic leukemia (T-PLL), BIBR 1532 shows selective cytotoxic effects in a dose-dependent manner and BIBR 1532-treated cells also demonstrates nuclear condensation and formation of apoptotic bodies morphologically compatible with apoptosis. [4] A recent study shows that combination treatment of BIBR 1532 and chemotherapeutic agents carboplatin results in a potential synergy for eliminateing ovarian cancer spheroid-forming cells in ES2, SKOV3, and TOV112D cell lines. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells NUDKSmNqTnWwY4Tpc44h[XO|YYm= NVTzUVFxOiCq M175U2lvcGmkaYTpc44hd2ZidHXsc41memG|ZTDpckBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEKgbJJ{KGK7IGvhcJBp[S1|MmDd[GdVWCCrbnPvdpBwemG2aX;uJIF{e2G7LDDJR|UxRTl|IH7N M3f5VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEGzPFQ2Lz5{MkSxN|g1PTxxYU6=
human MDA-MB-231 cells NH2xNnBHfW6ldHnvckBie3OjeR?= NEHnfFczPCCq MmruTY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFSUQWCtVGNTNUWOSWPBMEBKSzVyPUCuNVch|ryP NI\OWWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm2OVc4QCd-MkW5OlU4Pzh:L3G+
human MGC803 cells NFrUZmlHfW6ldHnvckBie3OjeR?= M3TmSVI1KGh? NV;hfJFkUW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJG1ISzhyMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhXFKDUD3QR3IuTUyLU1GsJGlEPTB;MD6yPEDPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NEmyNkc,OjV3NUS5NlI9N2F-
HeLa MUXGeY5kfGmxbjDhd5NigQ>? NHnidXQyPSCvaX7z NES3W4RKdmirYnn0bY9vKG:oIITlcI9u\XKjc3WgbY4hcHWvYX6gTIVN[SClZXzsd{B2e2mwZzC1K{1CSVRiQ1PHJHRETyCDR1OgRWdCKEeWVD2zK{BieyC|dXLzeJJifGViaX7jeYJifGWmIH\vdkAyPSCvaX7zJJBzcW:{IITvJIV5fGWwc3nvckBz\WGldHnvckBjgSC2ZXzvcYVzcWNicnXw[YF1KGGvcHzp[olk[XSrb36gdJJwfG:lb3ysJGlEPTB;Mz62{txO M4XDVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEGzPFQ2Lz5{MkSxN|g1PTxxYU6=
HeLa M3LsW2Z2dmO2aX;uJIF{e2G7 Ml3INVUhdWmwcx?= NVqwVGw2UW6qaXLpeIlwdiCxZjD0[YxwdWW{YYPlJIlvKGi3bXHuJGhmVGFiY3XscJMhfXOrbnegOUcuSUGWIFPDS{BVS0diQVfDJGFISSCJVGStN{ch[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhOTVibXnud{BxemmxcjD0c{BmgHSnboPpc44hemWjY4Tpc44h\m:ubH;3[YQh[nliY3;tdI92dmRid3HzbI92fCCkeTDzdIlvNXSnbH;t[ZJq[yC{ZYDlZZQh[W2ybHnmbYNifGmxbjDwdo91d2OxbDygTWM2OD12LkdOwG0> NGDyb489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSxN|g1PSd-MkK0NVM5PDV:L3G+
HeLa NH7DSHlHfW6ldHnvckBie3OjeR?= MWmzNEBucW6| MXnJcohq[mm2aX;uJI9nKGi3bXHuJJRmdG:vZYLhd4UhcXOxbHH0[YQh\nKxbTDoeY1idiCKZVzhJINmdGy|IH71Z4xm[XJiZYj0doFkfHNiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyCjc4Pld5Nm\CCjczDbN|NRZWSFTWCgbY5kd3Kyb4LheIlwdiCjZoTldkA{OCCvaX7zJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pIHHuZYx6e2m|LDDJR|UxRTBwMEmz{txO MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3M{W5Okc,OjRyNUO1PVY9N2F-
HEK293 MmTZSpVv[3Srb36gZZN{[Xl? M3vhTGlvcGmkaYTpc44hd2ZiaIXtZY4hfGWub33ldoF{\SCjY4Tpeol1gSCrc3;sZZRm\CCocn;tJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\E6WUDDpcoNwenCxcnH0bY9vKHW|aX7nJFUoNWKrb4Tpcplt[XSnZDDBRXRES0eWQ1fBS2NCT0GJVGSgdJJqdWW{IHL5JIZt[XOqIIDsZZRmKGG|c3H5MEBKSzVyPUOuOu69VQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ3N{iwPUc,Ojd4NUe4NFk9N2F-

... Click to View More Cell Line Experimental Data


Kinase Assay:


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Conventional Telomerase Assay :

For the direct telomerase assay with the endogenous telomerase, 10 μL of telomerase-enriched extract is mixed with different concentrations of BIBR1532 in a final volume of 20 μL. After 15-minute preincubation on ice, 20 μL of the reaction mixture is added, and the reaction is initiated by transferring the tubes to 37 °C. The final concentrations in the reaction mixture are 25 mM Tris-Cl (pH 8.3), 1 mM MgCl2, 1 mM EGTA, 1 mM dATP, 1 mM dTTP, 6.3 μM cold dGTP, 15 μCi [α-32P]dGTP (3000 Ci/mmol; NEN), 1.25 mM spermidine, 10 units of RNasin, 5 mM 2-mercaptoethanol, and 2.5 μM TS-primer (5
Cell Research:


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  • Cell lines: JVM13
  • Concentrations: 0 to 80 μM
  • Incubation Time: 24 -72 hours
  • Method:

    Cells are plated as triplicates in complete RPMI 1640 medium with various concentrations of BIBR1532. After 24 to 72 hours, water-soluble tetrazolium (WST-1) is added, which is transformed into formazan by mitochondrial reductase systems. The increase in the number of viable cells results in an increase of activity of mitochondrial dehydrogenases, leading to an increase of formazan dye formed, which is quantified by ELISA reader after 2, 3, and 4 hours of incubation.

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (199.17 mM)
Ethanol 3 mg/mL (9.05 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.36


CAS No. 321674-73-1
Storage powder
in solvent
Synonyms N/A
Smiles CC(=CC(=O)NC1=CC=CC=C1C(=O)O)C2=CC3=CC=CC=C3C=C2

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Does BIBR1532 diffuse through the plasma membrane and nuclear membrane?

  • Answer:

    BIBR1532 is a cell permeable molecule.

Telomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID