(-)-Epigallocatechin Gallate

Catalog No.S2250 Synonyms: EGCG

For research use only.

(-)-Epigallocatechin Gallate(EGCG) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.

(-)-Epigallocatechin Gallate Chemical Structure

CAS No. 989-51-5

Selleck's (-)-Epigallocatechin Gallate has been cited by 29 publications

Purity & Quality Control

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Biological Activity

Description (-)-Epigallocatechin Gallate(EGCG) is the main catechin extraction of green tea that inhibits telomerase and DNA methyltransferase. EGCG blocks the activation of EGF receptors and HER-2 receptors. ECGG inhibits fatty acid synthase and glutamate dehydrogenase activity.
Targets
telomerase [4]
()
DNMT [4]
()
HER2 [5]
()
EGFR [5]
()
FASN [6]
()
In vitro

(-)-Epigallocatechin gallate functions as a powerful antioxidant, preventing oxidative damage in healthy cells, but also as an antiangiogenic and antitumor agent and as a modulator of tumor cell response to chemotherapy. (-)-Epigallocatechin gallate shows multiple anticancer effects, such as anti-proliferation, anti-angiogenesis, transformation prevention of various cancer cells, cancer cell cycle arrest and inhibition of tumor metastasis. (-)-Epigallocatechin gallate exerts multi-anticancer effects through regulating several cancer-related cell signal pathways (regulated the function or the expression of key signal proteins, such as nuclear factor-κB, MAPKs and activator protein-1, EGFR, IGF, COX-2 signaling pathway, and so on.), effecting methylation of cancer genes and combination of ligand with membrane receptors. [1] (-)-Epigallocatechin gallate also shows an immunomodulating effects. Several types of immune cells in both the innate and adaptive immune systems are known to be affected in varying degrees by (-)-Epigallocatechin gallate. Among them, the dramatic effect on T cell functions has been repeatedly demonstrated, including T cell activation, proliferation, differentiation, and production of cytokines. Studies using animal models of autoimmune diseases have reported disease improvement in animals treated with green tea/EGCG. [2] (-)-Epigallocatechin gallate displays anti-infective properties. Antiviral activities of (-)-Epigallocatechin gallate with different modes of action have been demonstrated on diverse families of viruses, such as Retroviridae, Orthomyxoviridae and Flaviviridae and include important human pathogens like human immunodeficiency virus, influenza A virus and the hepatitis C virus. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SH-SY5Y cells MlvGSpVv[3Srb36gZZN{[Xl? NUf6NFdpVmW3cn;wdo91\WO2aX;uJIFo[Wmwc4SgZoV1[S2jbYnsc4llKHCncITp[IUhOS12Mj3pcoR2[2WmIITvfIlkcXS7IHnuJIh2dWGwIGPIMXN[PVliY3XscJMh[XO|ZYPz[YQh[XNibHHjeIF1\SCmZXj5[JJw\2WwYYPlJJJmdGWjc3WsJGVEPTB;MD6wN|k5PyEQvF2= M4HIXFE6OTN6OEW5
human MDA435/LCC6MDR cells MUTGeY5kfGmxbjDhd5NigQ>? MXexNEDPxE1? MlnWOUBl[Xm| NIj2Vo1Od2S3bHH0bY9vKG:oIGCt[5AhMHWwa37ve44hd3KrZ3nuLUB1emGwc3\lZ5Rm\CCrbjDoeY1idiCPRFG0N|UwVEOFNl3EVkBk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPpZoxmKG:oIIDhZ4xqfGG6ZXygdoV{cXO2YX7j[UBu\WG|dYLl[EBieyCLQ{WwJIZweiCyYXPsbZRigGWuIHH0JFExKHWPIHHmeIVzKDViZHH5d{BjgSCFZXzsWIl1\XJiOU[gRZF2\W:3czDhd5NigSxiSVO1NF0xNjF{Mk[g{txO MkXQNlU6QDVzOUW=
Sf9 cells NGPpRYhHfW6ldHnvckBie3OjeR?= NFLacmJKdmirYnn0bY9vKG:oIFjpd|YufGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGRPVVRzIHX4dJJme3OnZDDpckBqdnOnY4SgV4Y6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDEUmEhdWW2aInseJJidnOoZYLhd4Uh[WO2aY\peJkhfXOrbnegOUcu[mmxdHnufYxifGWmIES1MYJxKHWwbXX0bJlt[XSnZDDvdkBp\W2rbXX0bJlt[XSnZDDvcIlod263Y3zlc5Rq\GVic4Xid5Rz[XSnczDhcoQhYzOKXT3B[I9O\XRiYomgcIlyfWmmIIPjbY51cWyuYYTpc44h[2:3boTpcochdWW2aH;kMEBKSzVyPUCuOUDPxE1? NH3oZWozPTRyNkm0OC=>
human U937 cells MYXGeY5kfGmxbjDhd5NigQ>? MmfVTY5pcWKrdHnvckBw\iC2ZXzvcYVz[XOnIHnuJIh2dWGwIGW5N|ch[2WubIOsJGlEPTB;MTFOwG0> M1fUOVIzPDF|OES1
human HeLa cells MYHGeY5kfGmxbjDhd5NigQ>? M{\1TGlvcGmkaYTpc44hd2ZidHXsc41memG|ZTDpckBpfW2jbjDI[WxiKGOnbHzzJJV{cW6pIEWnMWFCXCCFQ1egWGNIKEGJQzDBS2EhT1SWLUOnJIF{KHO3YoP0doF1\SCrbnP1ZoF1\WRiZn;yJFE2KG2rboOgdJJqd3JidH:g[Zh1\W6|aX;uJJJm[WO2aX;uJIZwdGyxd3XkJIJ6KGOxbYDveY5lKHejc3jveZQh[nlic4Dpck11\WyxbXXybYMhemWyZXH0JIFueGyrZnnjZZRqd25icILveI9kd2xuIFnDOVA:OS5yODFOwG0> NHnkOJEzOjRzM{i0OS=>
CHO cells MnnzR5l1d3SxeHnjxsBie3OjeR?= MXi0PEBp NFGzbItEgXSxdH;4bYNqfHliYXfhbY5{fCCFSF:gZ4VtdHNiZYjwdoV{e2mwZzDPRXRROUJ|IHjhdIxwfHmyZTCxJIFnfGW{IES4JIhzeyCkeTDmcJVwemW|Y3XuZ4Uh[mG|ZXSgR4VtdFSrdHXyMWdtdyCjc4PhfUwhUUN3ME2zMlIh|ryP NYfaSoJ2OjN|Mke4O|c>
MDCK cells MljLSpVv[3Srb36gZZN{[Xl? NEf3S481KGSjeYO= MmD1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgbY5ndHWnborhJGEhfmm{dYOgLGEwe3erbnWvU2gwPTFzNES1M|IxODdqSEHONUkqKE:qNzDpcoZm[3SnZDDpckBOTEONIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yhfmm{YXygdoVxdGmlYYTpc44h[W[2ZYKgOEBl[Xm|IHL5JJF2[W62aYTheIl3\SCUVD3QR3ItKEWGNUC9PE4{KM7:TR?= NGXHfIEzOjFzNUW5NS=>
human HL60 cells NHzIUpRRem:uaX\ldoF1cW:wIHHzd4F6 NEPxVog{KGSjeYO= MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhOyCmYYnzMEBKSzVyPUmuOEDPxE1? NXi0WoFMOTh4OUOwNlA>
HSC-T6 cells  Ml7qSpVv[3Srb36gZZN{[Xl? NWnN[3QzPDhiaB?= NFK3Z4lCdnSrZnnido91cWNiYXP0bZZqfHliYXfhbY5{fCC{YYSgTHNENVR4IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheHKxbHnm[ZJifGmxbjDh[pRmeiB2ODDodpMh[nliQoLkWUBqdmOxcoDvdoF1cW:wIHHzd4F6NCCLQ{WwQVkvQSEQvF2= Mmf5NlE2ODR6NEi=
mouse 3T3-L1 cells MnfWSpVv[3Srb36gZZN{[Xl? M2L5XWlvcGmkaYTpc44hd2ZiR{\QSE1u\WSrYYTl[EBPSUSSSDDwdo9lfWO2aX;uJIlvKG2xdYPlJFNVOy2OMTDj[YxteyxiSVO1NF0zPSEQvF2= NUjkT3lFOTh|MUOzNFg>
mouse RAW264.7 cells MmrjSpVv[3Srb36gZZN{[Xl? NWDJZmlWPSCmYYnz NWHpN|NCUW6qaXLpeIlwdiCxZjDSRW5MVC2rbnT1Z4VlKG:|dHXvZ4xie3SxZ3Xu[ZNqeyCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDUVmFRNXCxc3n0bZZmKG23bITpMY52[2ynYYTl[EBk\WyuczDh[pRmeiB3IHThfZMtKEmFNUC9NlkvQCEQvF2= NHzpfnkzOTR3NkWyNS=>
HSC-T6 cells NWCzTm9WWHKxbHnm[ZJifGmxbjDhd5NigQ>? NEHIb5NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JJJifCCKU1OtWFYh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6NCCLQ{WwQVI6NjhizszN NYnRfm0yOjV|MkK0OVU>
human A431 cells NE\G[3lRem:uaX\ldoF1cW:wIHHzd4F6 M2O2T|Q5KGh? MnvVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNEOxJINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEW{YlKgbY4he2W{dX2t[pJm\SCvZXTpeY0h[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JHdUXC1zIHHzd4F6NCCHQ{WwQVM5KM7:TR?= MoX0NlQ1PTZyMES=
human MDA-MB-231 cells M3TlNHBzd2yrZnXyZZRqd25iYYPzZZk> NWjvV2IyOjRiaB?= NGn3WWtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>? NFexOXAzOjR3OUKwPC=>
human HepG2 cells NVv0dXlZTnWwY4Tpc44h[XO|YYm= NULr[4NQOjRiaB?= MUjJcohq[mm2aX;uJI9nKG:uZXnjJIFkcWRvaX7keYNm\CC2cnnncJlk\XKrZHWgc5Zmei2jY3P1cZVt[XSrb36gbY4hcHWvYX6gTIVxTzJiY3XscJMhcW6ldXLheIVlKG[xcjCyOEBpenNicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3y= NGrPdYgzOTh{NE[5NC=>
human HepG2 cells MYPGeY5kfGmxbjDhd5NigQ>? NXnXc3BqOjRiaB?= MmLTRY51cW:6aXThcpQh[WO2aY\peJkhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKG:uZXnjJIFkcWRvaX7keYNm\CCUT2Og[4Vv\XKjdHnvckBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDETGNHNUSDIHLhd4VlKG[udX;ybY1mfHKrYzDhd5NigSC{ZXzheIl3\SC2bzD1cpRz\WG2ZXSgZ49vfHKxbB?= MWCyNVgzPDZ7MB?=
human Caco-2 cells NVvlZWZNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHnGTlEzPSEQvF2= MUi2JIRigXN? NGHYcnJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBE[WOxLUKgZ4VtdHNiYYSgNlUhfU1iYX\0[ZIhPiCmYYnz NV7IZZA3OTh|MkS3OlM>
mouse 3T3 cells MmHWSpVv[3Srb36gZZN{[Xl? MXyxMVIxKM7:TR?= MWmxNkBp NIPxRnJCdnSrbXnndoF1d3K7IHHjeIl3cXS7IHHnZYlve3RiU4fpd5Mh[WykaX7vJI1wfXOnIEPUN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBw\iClZXzsJI52dWKncoOgZZQhOSC2bzCyNEB2VSCjZoTldkAyOiCqcoOgZpkh[nlic3PyZZRkcC25b4Xu[EBie3OjeR?= MoXpNlExQDB4NEK=
human MDA-MB-231 cells NGPs[JZRem:uaX\ldoF1cW:wIHHzd4F6 MX[yOEBp MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfS=> NGnMdIUzOjR3OUKwPC=>
human Jurkat cells MkPLSpVv[3Srb36gZZN{[Xl? MofFNVAuPTVizszN M4rMUVI1KGh? MnjMTY5pcWKrdHnvckBw\iCWTl\hcJBp[S2rbnT1Z4VlKE6ILXvhdJBiSiCjY4TpeoF1cW:wIHnuJIh2dWGwIFr1dotifCClZXzsd{BifCBzMDD0c{A2PSC3TTDh[pRmeiB{NDDodpMh[nliZXzlZ5Rzd3Cqb4LleIlkKG2xYnnsbZR6KHOqaX\0JIF{e2G7 NX;sfWNoOjFyOEC2OFI>
human K562 cells NVnubJhvTnWwY4Tpc44h[XO|YYm= M33kTVI1KGh? MnPyTY5lfWO2aX;uJI9nKDZ5IHvEZUBt[W2rbnnuJJJm[2WydH;yJIV5eHKnc4Ppc44hcW5iaIXtZY4hUzV4MjDj[YxteyCjZoTldkAzPCCqcoOgZpkh\myxdzDjfZRwdWW2comgZY5idHm|aYO= NVfwRYVFOjF2M{S2NFM>
human HL60 cells NITZb4ZHfW6ldHnvckBie3OjeR?= MmX5NlQhcA>? NFPjbnVKdmS3Y4Tpc44hd2ZiNkegb2RiKGyjbXnubY4hemWlZYD0c5Ih\XiycnXzd4lwdiCrbjDoeY1idiCKTE[wJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCobH;3JIN6fG:vZYTyfUBidmGueYPpdy=> NYT4eJJ[OjF2M{S2NFM>
human Raji cells M1TGRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{HWVFExKM7:TR?= MofEOFghcA>? NHTQbGlIem:5dHigbY5pcWKrdHnvckBi\2GrboP0JIh2dWGwIGLhbokh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IITyfZBidiCkbIXlJIJie2WmIH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzJIlvKHC{ZYPlcoNmKG:oIIP1dIVzd3irZHWg[Il{dXW2YYPl NUnpcW1WOjF2M{S2NFM>
human PC3 cells MWDQdo9tcW[ncnH0bY9vKGG|c3H5 M3rxRlExNTFyMDFOwG0> MoTxOFghcA>? MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjdDCxNEB1dyBzMECgeW0h[W[2ZYKgOFghcHK|IHL5JIhmdW:leYTvcYV1emmlIHPlcIwh[2:3boTpcochdWW2aH;k MUOyN|g3PzF4Nx?=
human A431 cells MkK3SpVv[3Srb36gZZN{[Xl? MlTaNVAxKM7:TR?= M1TTVVEzKGh? MXHS[YR2[3Srb36gc4Yh[2y3c4Tldolv\yCxZjDHSnAuT1CLIHnuJIxqeGmmIILh[pR{KG:oIHj1cYFvKEF2M{GgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDF{IHjyd{BjgSClb37mc4NidCCvaXPyc5Nkd3CrYzDhcoFtgXOrcx?= M3;Q[VI1PDV4MEC0
human SKBR3 cells NHjGOVZHfW6ldHnvckBie3OjeR?= NIjFbVgzODEQvF2= M{X3bVMxKG2rboO= MYfEc5dvemWpdXzheIlwdiCxZjDFdoJDOiCycn;0[YlvKGW6cILld5Nqd25iaX6gbJVu[W5iU1vCVlMh[2WubIOgbY4he2W{dX2g[pJm\SCvZXTpeY0h[XRiMkCwJJVOKGGodHXyJFMxKG2rboOgZpkhcW2vdX7v[ox2d3Knc3PlcoNmKHO2YXnubY5oNWKjc3XkJINwdm[xY3HsJI1q[3Kxc3PvdIlkKGGwYXz5d4l{ MnPvNlQ1PTZyMES=
human 293T cells NXO2XGlZTnWwY4Tpc44h[XO|YYm= M3LpfFUxKM7:TR?= MoXuNVIhcA>? MnT4TY5pcWKrdHnvckBw\iCGWWLLNWEhcW5iaIXtZY4hOjl|VDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhT0yLMTD0doFve2O{aYD0bY9vKGGldHn2bZR6KGG2IEWwJJVOKGGodHXyJFEzKGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NWewbIhHOjJzNUS2OlQ>
Assay
Methods Test Index PMID
Western blot Notch1 / Notch2 ; p-MAPK / MAPK ; β-catenin / p-AKT / Cyclin D1 28443100 30233784 28693189
Growth inhibiton assay Cell viability ; Cell proliferation 28693189 23525843

Protocol (from reference)

Solubility (25°C)

In vitro

Ethanol 83 mg/mL warmed
(181.07 mM)
DMSO 72 mg/mL warmed
(157.07 mM)
Water 23 mg/mL warmed
(50.17 mM)

Chemical Information

Molecular Weight 458.37
Formula

C22H18O11

CAS No. 989-51-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

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% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03928847 Recruiting Drug: Epigallocatechin-3-gallate (EGCG) Idiopathic Pulmonary Fibrosis Hal Chapman|National Heart Lung and Blood Institute (NHLBI)|University of California San Francisco July 1 2018 Early Phase 1
NCT02779361 Completed Dietary Supplement: Green tea Healthy Volunteers|Doping in Sports Parc de Salut Mar|World Anti-doping Agency January 2016 Phase 1
NCT02577393 Enrolling by invitation Drug: EGCG|Drug: mLDG Lung Neoplasms|Esophagitis|Prevention & Control|Epigallocatechin Gallate Shandong Cancer Hospital and Institute April 2015 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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