Remdesivir (GS-5734)

Catalog No.S8932

For research use only.

Remdesivir (GS-5734), a monophosphoramidate prodrug of an adenosine analog, is an investigational broad-spectrum antiviral agent with in vitro activity against multiple RNA viruses, including Ebola and CoV.

Remdesivir (GS-5734) Chemical Structure

CAS No. 1809249-37-3

Selleck's Remdesivir (GS-5734) has been cited by 65 publications

Purity & Quality Control

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Biological Activity

Description Remdesivir (GS-5734), a monophosphoramidate prodrug of an adenosine analog, is an investigational broad-spectrum antiviral agent with in vitro activity against multiple RNA viruses, including Ebola and CoV.
In vitro

GS-5734 exhibits antiviral activity against multiple variants of EBOV in cell-based assays(EC50=0.06-0.14 μM) and broad-spectrum antiviral activity in vitro against other pathogenic RNA viruses. [1]GS-5734 acts as a broad-spectrum therapeutic to protect against CoVs with EC50 of 0.03 μM for murine hepatitis virus in delayed brain tumor cells and 0.074 μM for SARS-CoV and MERS-CoV in HAE cells.[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Hep2 NFzrNXZCdnSrdnnyZYwh[WO2aY\peJkh[XO|YYm= NGPROGM1KGSjeYO= NXTHWlVtTUN3MDC9JFAvODF3IN88US=> M1robVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUK0PVA4Lz5{OEGyOFkxPzxxYU6=
TERT-immortalized HMVEC MW\BcpRqfmm{YXygZYN1cX[rdImgZZN{[Xl? NHL1eXQ{KHSxIESg[IF6ew>? NX;zTpdFTUN3MDC9JFAvODV|IN88US=> NVrJRXhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixNlQ6ODdpPkK4NVI1QTB5PD;hQi=>
HuH7 MXrBcpRqfmm{YXygZYN1cX[rdImgZZN{[Xl? MUmzJIRigXN? MXLFR|UxKD1iMD6wOVch|ryP M{nnTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUK0PVA4Lz5{OEGyOFkxPzxxYU6=
HuH7 NVnwb|VCSW62aY\pdoFtKGGldHn2bZR6KGG|c3H5 MYizJIRigXN? M2jp[2VEPTBiPTCwMlA4KM7:TR?= MkXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OUK3OlMoRjJ6N{myO|Y{RC:jPh?=
macrophages NFWybHNCdnSrdnnyZYwh[WO2aY\peJkh[XO|YYm= MmjFOFghcA>? NYPXTJE1TUN3MDC9JFAvODh4IN88US=> MmrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzMkS5NFcoRjJ6MUK0PVA4RC:jPh?=
macrophages MkHWRY51cX[rcnHsJIFkfGm4aYT5JIF{e2G7 NUP1N2ttPDhiaB?= Mn\xSWM2OCB;IECuNFg3KM7:TR?= Ml3FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OUK3OlMoRjJ6N{myO|Y{RC:jPh?=
HeLa NHvUSYZCdnSrdnnyZYwh[WO2aY\peJkh[XO|YYm= NXfL[lZzPDhiaB?= NVfBRZdDTUN3MDC9JFAvOSEQvF2= MmXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzMkS5NFcoRjJ6MUK0PVA4RC:jPh?=
HeLa MYnBcpRqfmm{YXygZYN1cX[rdImgZZN{[Xl? MX20PEBp NGDiRpVGSzVyIE2gNE4yPCEQvF2= M13GR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6N{myO|Y{Lz5{OEe5Nlc3OzxxYU6=
MT4 NFzvRZJEgXSxdH;4bYNqfHliYYPzZZk> MVy0JJRwKDViZHH5dy=> M{\jVmNEPTBiPTCxMlch|ryP NULrO|B2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixNlQ6ODdpPkK4NVI1QTB5PD;hQi=>
Hep2 MXfDfZRwfG:6aXPpeJkh[XO|YYm= NVTNW4tWPCC2bzC1JIRigXN? M2HjbWNEPTBiPTC2MlEh|ryP NWK5S2sxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixNlQ6ODdpPkK4NVI1QTB5PD;hQi=>
HuH7 MXLDfZRwfG:6aXPpeJkh[XO|YYm= Mm\SN{Bl[Xm| MnXyR2M2OCB;IEO2JO69VQ>? M4ryWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUK0PVA4Lz5{OEGyOFkxPzxxYU6=
Assay
Methods Test Index PMID
Western blot delayed chain termination of RNA synthesis ; tGFP-BlaR / Actin ; ACE2 / Actin ; VP0 / VP2 30987343 33835389 32793908 32595613
Growth inhibition assay Cell viability ; Cell viability ; Cell viability 33835389 33186749 32595613
Pharmacokinetics plasma concentration ; plasma concentration 32589775 33782830
Immunofluorescence MERS-CoV S Protein ; viral S protein and E-caherin ; SARS-CoV-2 / E‐cadherin / VE‐cadherin ; SARS-CoV-2 ; viral dsRNA replication intermediates 33376043 33186749 33173719 32869028 32595613
Viral loads and virus titers SARS-CoV-2 32516797
In vivo

Regardless of the time of initiation, GS-5734 treatment confers improved survival when administered by 3 mg/kg GS-5734. All animals in which 10 mg/kg GS-5734 treatments is initiated 3 days after virus exposure survive to the end of the in-life phase. However, the antiviral effects are consistently greater in animals administered repeated 10 mg/kg GS-5734 doses. The 10 mg/kg D3 (administered beginning 3 days after virus exposure) GS-5734 regimen is associated with amelioration of EVD-related clinical disease signs and markers of coagulopathy and end organ pathophysiology.[1]

Protocol (from reference)

Animal Research:

[1]

  • Animal Models: Rhesus monkeys (Macaca mulatta)
  • Dosages: 3 mg / kg, 10 mg / kg
  • Administration: IV

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 602.58
Formula

C27H35N6O8P

CAS No. 1809249-37-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(CC)COC(=O)C(C)NP(=O)(OCC1C(C(C(O1)(C#N)C2=CC=C3N2N=CN=C3N)O)O)OC4=CC=CC=C4

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Tech Support

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