NGI-1 (ML414)

For research use only.

Catalog No.S8750

1 publication

NGI-1 (ML414) Chemical Structure

CAS No. 790702-57-7

NGI-1 (ML414) is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.

Selleck's NGI-1 (ML414) has been cited by 1 publication

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Biological Activity

Description NGI-1 (ML414) is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.
Targets
oligosaccharyltransferase (OST) [1]
()
In vitro

NGI-1 is a reversible catalytic subunit inhibitor of the oligosaccharyltransferase (OST) that has higher specificity for STT3B compared to STT3A (STT: OST catalytic subunit). In non-small cell lung cancer cells NGI-1 blocks cell surface localization and signaling of the EGFR glycoprotein, but selectively arrests proliferation in only those cell lines that are dependent on EGFR (or FGFR) for survival. In these cell lines OST inhibition causes cell cycle arrest accompanied by induction of p21, autofluorescence, and changes in cell morphology; all hallmarks of senescence[1]. NGI-1 exhibits broad antiviral activity against multiple flaviviruses. It blocks viral RNA replication independent of inhibition of the catalytic activity of the OST complex[2].

Protocol

Cell Research:

[1]

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  • Cell lines: PC9 and A549 cells
  • Concentrations: 10 μM
  • Incubation Time: 24 h
  • Method:

    PC9 and A549 cells were cultured in 6-well plates in serum-containing medium and treated with or without 10 μM NGI-1 for 24 hours.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 79 mg/mL (200.24 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 394.51
Formula

C17H22N4O3S2

CAS No. 790702-57-7
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CN=C(S1)NC(=O)C2=C(C=CC(=C2)S(=O)(=O)N(C)C)N3CCCC3

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID