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U18666A Antiviral inhibitor

Name: U18666A
Brand: Selleck Chemicals
SKU: S9669
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S9669

U18666A (U18) is an inhibitor of intra-cellular cholesterol transport that inhibits dengue virus entry and replication.

Chemical Structure

Molecular Weight: 424.06

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Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	PD-1/PD-L1 CXCR STING AhR Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX
Other Antiviral Products	Moroxydine HCl Aloperine GS-441524 Oleanolic Acid NGI-1 Lapachol Saikosaponin B2 Harringtonine Carrageenan Goitrin

Solubility

In vitro
Batch:

DMSO : 85 mg/mL (200.44 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 85 mg/mL

Water : 43 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.59mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (0.59mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	424.06	Formula	C₂₅H₄₂ClNO₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	3039-71-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	U18			Smiles	CCN(CC)CCOC1CCC2(C3CCC4(C(C3CC=C2C1)CCC4=O)C)C.Cl

Mechanism of Action

In vitro	Viral particles are trapped in the Lamp-1 positive late endosome/lysosome compartment when cells are treated with U18666A. In addition, viral replication is also affected by this compound treatment. An additive anti-viral effect is found when C75, a fatty acid synthase inhibitor, is used in combination with this chemical, suggesting the role of cholesterol and fatty acid in DENV.
In vivo	When U18666A is administered to cats experimentally infected with type I FIPV, the development of FIP may be suppressed compared with the control group. However, the number of animals with FIP is too low to establish anti-viral effect of this compound in cats. Two of the five control cats administered PBS alone develops FIP. Four of the five cats administered this chemical develops no signs of FIP. One cat that temporarily developed fever, has no other clinical symptoms, and no gross lesion is noted on an autopsy after the end of the experiment. The FIPV gene is detected intermittently in feces and saliva regardless of the development of FIP or administration of this compound.
References	[1] https://pubmed.ncbi.nlm.nih.gov/22146564/ [2] https://pubmed.ncbi.nlm.nih.gov/31963705/

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