U18666A

Catalog No.S9669 Synonyms: U18

For research use only.

U18666A (U18) is an inhibitor of intra-cellular cholesterol transport that inhibits dengue virus entry and replication.

U18666A Chemical Structure

CAS No. 3039-71-2

Purity & Quality Control

Choose Selective Antiviral Inhibitors

Biological Activity

Description U18666A (U18) is an inhibitor of intra-cellular cholesterol transport that inhibits dengue virus entry and replication.
In vitro

Viral particles are trapped in the Lamp-1 positive late endosome/lysosome compartment when cells are treated with U18666A. In addition, viral replication is also affected by U18666A treatment. An additive anti-viral effect is found when C75, a fatty acid synthase inhibitor, is used in combination with U81666A, suggesting the role of cholesterol and fatty acid in DENV.[1]

In vivo

When U18666A is administered to cats experimentally infected with type I FIPV, the development of FIP may be suppressed compared with the control group. However, the number of animals with FIP is too low to establish anti-viral effect of U18666A in cats. Two of the five control cats administered PBS alone develops FIP. Four of the five cats administered U18666A develops no signs of FIP. One cat that temporarily developed fever, has no other clinical symptoms, and no gross lesion is noted on an autopsy after the end of the experiment. The FIPV gene is detected intermittently in feces and saliva regardless of the development of FIP or administration of U18666A.[2]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: BHK21 cells
  • Concentrations: 6.15 μM
  • Incubation Time: 48 h
  • Method:

    BHK21 cells are seeded with a cell density of 2×104 cells/well into a 96 well plate 1 day before infection. NGC virus is added at a multiplicity of infection (MOI) of 1 in the presence of 2% FBS and is harvested from the cell supernatant 2 days post infection. Viral titer is quantified by plaque assay. For time of addition experiment of U18666A, cells are pretreated for 16 h with the compound, before virus is added to the cells (16 h pretreatment) or cells are pretreated for 16 h and the compound remained throughout the 48 h of infection (throughout). Alternatively, compound is added only during the 1st hour of infection where most of the entry and fusion occurred (1 h entry), or after the 1st hour of infection (after entry) for the remaining of the infection.

Animal Research:

[2]

  • Animal Models: SPF cats
  • Dosages: 2.5 mg/kg, 1.25 mg/kg
  • Administration: SC

Solubility (25°C)

In vitro

Water 40 mg/mL
(94.32 mM)
DMSO 30 mg/mL
(70.74 mM)
Ethanol '''''85 mg/mL

Chemical Information

Molecular Weight 424.06
Formula

C25H42ClNO2

CAS No. 3039-71-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCN(CC)CCOC1CCC2(C3CCC4(C(C3CC=C2C1)CCC4=O)C)C.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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