Name: GS-441524
Brand: Selleck Chemicals
SKU: S6814
Availability: InStock
Rating: 5 (8 reviews)

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Quality Control

Batch: Purity: 99.88%

99.88

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal COVID-19 Parasite Reverse Transcriptase HIV HCV Protease
Other Antiviral Inhibitors	Moroxydine HCl Aloperine Harringtonine Oleanolic Acid NGI-1 U18666A Aloin B Lapachol LL-37 acetate Saikosaponin B2

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HuH7	Function assay	10 uM	24 hrs	Cmax in human HuH7 cells assessed as triphosphate concentration per million cells at 10 uM incubated for 24 hrs measured after washout by LC-MS analysis, Cmax=0.0000176μM	22446091
Vero	Antiviral assay		24 hrs	Antiviral activity against Parainfluenza 3 C 243 infected in african green monkey Vero cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=1.71μM	22446091
Vero	Antiviral assay		24 hrs	Antiviral activity against SARS coronavirus Toronto-2 infected in african green monkey Vero cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=2.24μM	22446091
HuH7	Antiviral assay		24 hrs	Antiviral activity against Hepatitis C virus genotype 1b infected in human HuH7 cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=4.1μM	22446091
Vero E6	Antiviral assay		24 hrs	Antiviral activity against Dengue virus type 2 New Guinea C infected in african green monkey Vero E6 cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=9.46μM	22446091
HeLa	Antiviral assay		24 hrs	Antiviral activity against Yellow fever virus 17D infected in human HeLa cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=11μM	22446091
MDCK	Antiviral assay		24 hrs	Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=27.9μM	22446091
Hep2	Antiviral assay		4 days	Antiviral activity against Respiratory syncytial virus A2 infected in human Hep2 cells assessed as reduction of virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay, EC50=0.53μM	28124907
HMVEC	Antiviral assay		3 to 4 days	Antiviral activity against GFP-fused Ebolavirus infected in TERT-immortalized HMVEC assessed as reduction in viral replication preincubated with cells followed by viral infection measured after 3 to 4 days by fluorescence assay, EC50=0.78μM	28124907
HuH7	Antiviral assay		3 days	Antiviral activity against Hepatitis C virus genotype 1b infected in human HuH7 cells preincubated with cells for 3 days followed by viral infection by luciferase assay, EC50=4.1μM	28124907
HuH7	Antiviral assay		3 days	Antiviral activity against Ebolavirus infected in human HuH7 cells after 3 days by end point dilution assay, EC50=1.5μM	28792763
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 58 mg/mL (199.13 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.900mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 58 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.725mg/ml (2.49mM)	Taking the 1 mL working solution as an example, add 50 μL of 14.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	291.26	Formula	C₁₂H₁₃N₅O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1191237-69-0	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=C2C(=NC=NN2C(=C1)C3(C(C(C(O3)CO)O)O)C#N)N

Mechanism of Action

Targets/IC₅₀/Ki	FIPV (Cell-free assay) 0.78 μM(EC50)
References	[1] https://pubmed.ncbi.nlm.nih.gov/29778200/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05996744	Terminated	COVID-19	Gilead Sciences	December 26 2023	Phase 2\|Phase 3
NCT05715528	Completed	COVID-19	Gilead Sciences	February 8 2023	Phase 3
NCT05603143	Terminated	COVID-19	Gilead Sciences	November 5 2022	Phase 3
NCT04582266	Completed	COVID-19	National Institute of Allergy and Infectious Diseases (NIAID)\|Gilead Sciences	March 31 2021	--
NCT04859244	Completed	COVID-19	Copycat Sciences LLC	January 1 2021	Phase 1
NCT04539262	Completed	COVID-19	Gilead Sciences	September 14 2020	Phase 1\|Phase 2

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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GS-441524 Antiviral inhibitor

Chemical Structure

Molecular Weight: 291.26