PF-00562271 Besylate

For research use only.

Catalog No.S2672 Synonyms: PF-562271 Besylate

15 publications

PF-00562271 Besylate Chemical Structure

CAS No. 939791-38-5

PF-00562271 Besylate (PF-562271) is the benzenesulfonate salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.

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Selleck's PF-00562271 Besylate has been cited by 15 publications

4 Customer Reviews

  • Mol Ther 2012 20(5), 972-83. PF-00562271 Besylate purchased from Selleck.

  • Pharmacological inhibition of optoFAK with the ATP-competitive FAK inhibitor PF-00562271. OptoFAK expressing SC4 cells were serum-starved for 24 h in the absence or presence of PF-00562271 (1 mM) before being illuminated for 10 min with blue light (451 nm, 2 μmol m-2 s-1) or kept in the dark.

    Cell Signal, 2018, 42:176-183. PF-00562271 Besylate purchased from Selleck.

  • The migration index enhanced by CX3CL1 was dramatic reduced using Bosutinib and PF-00562271. CX3CL1-only group as control. Scale bar = 200 μm. The experiments were repeated three times. *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001.

    J Cancer, 2018, 9(19):3603-3612. PF-00562271 Besylate purchased from Selleck.

  • SGC-7901 and MGC-803 cells were treated with OLFM4-sh lentivirus or FAK inhibitor (PF) alone, or co-treated with OLFM4-sh lentivirus and PF. Cellular invasive ability was measured by transwell assay after indicated treatment. Data are expressed as mean ± standard deviation from three independent experiments. One was analysis of variance (ANOVA) with Bonferroni T post-test was used to analysis the data. *P < 0.05, ***P < 0.001; ##P < 0.01, ###P < 0.001 VS. OLFM4-sh group.

    BMB Rep, 2015, 48(11):630-5. PF-00562271 Besylate purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-00562271 Besylate (PF-562271) is the benzenesulfonate salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 Besylate shows the selective inhibitory effects on FAK and Pyk2 tyrosine kinase activity with IC50 of 1.5 nM and 14 nM, respectively. And in cell-based assays, the IC50 of PF-562271 is shown to be 5 nM for FAK, which is more selective compared to other kinase targets. [1] In 2 dimensional (2D) cultures, PF-562271 results in a dose-dependent cell proliferation inhibition in FAK WT, FAK−/− and FAK kinase-deficient (KD) cells with IC50 of 3.3 μM, 2.08 μM and 2.01 μM, respectively. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 cell M4nnT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXLlSoxJUW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5{N{[2JO69VQ>? M4r5OXNCVkeHUh?=
human SW982 cell M1HJemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYfJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6zNlgzKM7:TR?= MmDZV2FPT0WU
human KM12 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFG0XlRKdmirYnn0bY9vKG:oIHj1cYFvKEuPMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlM5PTV5IN88US=> M{LRO3NCVkeHUh?=
human COLO-205 cell MoDrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2PUPWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz2yNFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjR6NkW4JO69VQ>? NIT6VYxUSU6JRWK=
human COLO-829 cell M1v6NGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGjLTm1KdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tPFI6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55NkG3OkDPxE1? M{jKbnNCVkeHUh?=
human MG-63 cell NVflcI5XT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3jhPWlvcGmkaYTpc44hd2ZiaIXtZY4hVUdvNkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgxPjN5IN88US=> M4HoOXNCVkeHUh?=
human IGROV-1 cell NXnTNYR6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgyODN6IN88US=> NWrIPJdUW0GQR1XS
human NCI-H650 cell M1r3e2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV;Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNkWwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45OzF3NDFOwG0> NELRO3JUSU6JRWK=
human RT-112 cell NVG2e2pNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NW\0OFZUUW6qaXLpeIlwdiCxZjDoeY1idiCUVD2xNVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjl6NE[g{txO NWGzN4hwW0GQR1XS
human BCPAP cell M3zqU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUjtbIJ3UW6qaXLpeIlwdiCxZjDoeY1idiCEQ2DBVEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODF{OEig{txO NHLCcmNUSU6JRWK=
ALL-PO cell NX\2W21VT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\wT3ZjUW6qaXLpeIlwdiCxZjDoeY1idiCDTFytVG8h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjBzNUi0JO69VQ>? NXH0U4gxW0GQR1XS
human KYSE-270 cell NGHDXWlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\tbGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT2yO|Ah[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjB2N{G0JO69VQ>? Mn3WV2FPT0WU
human 8305C cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJFg{ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6wPVkxPCEQvF2= MnPXV2FPT0WU
NCI-H810 cell NEXhXWFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnT2TY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEixNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTB5N{[g{txO NHP2RpNUSU6JRWK=
human CAL-33 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{nJOWlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUOzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4yOjl|ODFOwG0> M2XOOHNCVkeHUh?=
human AN3-CA cell Mmn0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGFPOy2FQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlE5PjdizszN M32xcnNCVkeHUh?=
human NKM-1 cell NYTyVJg1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mnv4TY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlc2ODZizszN NUX3NYVWW0GQR1XS
human BPH-1 cell NHHQOGxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPZTY5pcWKrdHnvckBw\iCqdX3hckBDWEhvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlg4PjZizszN MmnrV2FPT0WU
human MES-SA cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M125ZWlvcGmkaYTpc44hd2ZiaIXtZY4hVUWVLWPBJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{ODZ6MjFOwG0> M3;KXXNCVkeHUh?=
human CAL-62 cell M4nHXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn:5TY5pcWKrdHnvckBw\iCqdX3hckBESUxvNkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlMyQTB7IN88US=> NGPaS2pUSU6JRWK=
human KYSE-150 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoXPTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUG1NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzV{M{[g{txO MlPaV2FPT0WU
human SK-UT-1 cell NF\kOmtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MonQTY5pcWKrdHnvckBw\iCqdX3hckBUUy2XVD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41PDZ2NzFOwG0> MUTTRW5ITVJ?
human HUTU-80 cell M1rGTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYKxTXVqUW6qaXLpeIlwdiCxZjDoeY1idiCKVWTVMVgxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS52NEi4OkDPxE1? MVHTRW5ITVJ?
human SIG-M5 cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGLVUVRKdmirYnn0bY9vKG:oIHj1cYFvKFOLRz3NOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDh2OEeg{txO NXjKbGd4W0GQR1XS
human AGS cell MXvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;KS3RnUW6qaXLpeIlwdiCxZjDoeY1idiCDR2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlUzOTJ2IN88US=> NGO2[ZZUSU6JRWK=
human ST486 cell NHfTfZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Moe4TY5pcWKrdHnvckBw\iCqdX3hckBUXDR6NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVMzPzhizszN NX74copnW0GQR1XS
human HSC-2 cell NYP6dnk6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{e1[WlvcGmkaYTpc44hd2ZiaIXtZY4hUFOFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlU{QTVizszN M1v4b3NCVkeHUh?=
human BC-1 cell NH7vV29Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYjkcm5CUW6qaXLpeIlwdiCxZjDoeY1idiCEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43OTZ4NDFOwG0> NEjKOHNUSU6JRWK=
human CGTH-W-1 cell M3zwS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTwPIlKdmirYnn0bY9vKG:oIHj1cYFvKEOJVFitW{0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS54MU[3PUDPxE1? MmO1V2FPT0WU
human MZ1-PC cell M336cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYXJcohq[mm2aX;uJI9nKGi3bXHuJG1bOS2SQzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlI{OTJizszN MVTTRW5ITVJ?
human SW1710 cell NXy2VWZ2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mlj2TY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlYzPjJ6IN88US=> NV3OZoh7W0GQR1XS
human EW-13 cell M1TjXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTLTYtKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43OzR4NjFOwG0> M1\rWXNCVkeHUh?=
human U251 cell NFOxbmFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2rES2lvcGmkaYTpc44hd2ZiaIXtZY4hXTJ3MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuO|QxOzFizszN NYTFbFg4W0GQR1XS
human NCI-H460 cell NVzFNIRDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYfaTmcyUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFQ3OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMES4N|kh|ryP M2TG[3NCVkeHUh?=
human DU-4475 cell M4D2N2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGRWNTR2N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlE1PzV7IN88US=> NUDLOVY3W0GQR1XS
human MFE-296 cell NVTicXBDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1XxXmlvcGmkaYTpc44hd2ZiaIXtZY4hVU[HLUK5OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPDd5OUKg{txO MlTYV2FPT0WU
human DU-145 cell M{LGemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuOFkyOThizszN NVHkUW1[W0GQR1XS
human MDA-MB-231 cell M2DBTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGPXSWtKdmirYnn0bY9vKG:oIHj1cYFvKE2GQT3NRk0zOzFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkS5OVczKM7:TR?= M3\iUHNCVkeHUh?=
human SNU-387 cell NWroNlY6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmrUTY5pcWKrdHnvckBw\iCqdX3hckBUVlVvM{i3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk42Ojh{IN88US=> NFzxXYtUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo In several human s.c. xenograft models, PF-562271 exhibits dose-dependent tumor growth inhibition, and produces maximum tumor inhibition for PC-3M, BT474, BxPc3, and LoVo ranging from 78% to 94% inhibition at doses of 25 to 50 mg/kg twice daily, without weight loss, morbidity, or death. [1] PF-562271 (25 mg/kg by p.o.) leads to a significant decrease in tumor progression in both subcutaneous and bone metastasis PC3M-luc-C6 xenograft models. [3] In a Huh7.5 hepatocellular carcinoma xenograft model, combination therapy of sunitinib and PF-562271 targets angiogenesis and tumor aggressiveness, and produces more significant anti-tumor effect than single agent by blocking tumor growth and impacting the ability of the tumor to recover upon withdrawal of the therapy. [4]

Protocol

Kinase Assay:

[1]

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Recombinant kinase assay and enzyme kinetics :

Briefly, purified-activated FAK kinase domain (amino acid 410–689) is reacted with 50 μM ATP and 10 μg per well of a random peptide polymer of Glu and Tyr, p(Glu/Tyr), in kinase buffer [50 mM HEPES (pH 7.5), 125 mM NaCl, and 48 mM MgCl2] for 15 minutes. Phosphorylation of p(Glu/Tyr) is challenged with serially diluted PF-562271 at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is tested in triplicate. Phosphorylation of p(Glu/Tyr) is detected with a general antiphospho-tyrosine (PY20) antibody followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody. HRP substrate is added, and absorbance readings at 450 nm are obtained after addition of stop solution (2 M H2SO4). IC50 values are determined using the Hill-Slope Model. Broad kinase selectivity profiling is performed in house and by using the KinaseProfiler Selectivity Screening Service available through UpState Biotechnology.
Cell Research:

[2]

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  • Cell lines: Squamous cell carcinoma (SCC)
  • Concentrations: 0 to 1 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are plated for 48 hours before addition of PF-562271. After 3 days cells are fixed by addition of ice cold 25% trichloroacetic acid (TCA) solution prior to staining with Sulforhodamine B (SRB) dye solution. Plates are washed with 1% glacial acetic acid, air-dried and resuspended in 10 mM Tris buffer, pH 10.5 before reading absorbance at 540 nm. Curve fitting and generation of IC50 values is carried out using GraphPad Prism 4 software from six replicates.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: PC-3M, BT474, BxPc3, LoVo, U87MG, H125 and H460 cells are injected s.c. into the right flank of athymic female mice .
  • Dosages: ≤100 mg/kg
  • Administration: Administered via p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (21.03 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 665.66
Formula

C21H20F3N7O3S.C6H6O3S

CAS No. 939791-38-5
Storage powder
in solvent
Synonyms PF-562271 Besylate
Smiles CN(C1=C(C=CC=N1)CNC2=NC(=NC=C2C(F)(F)F)NC3=CC4=C(C=C3)NC(=O)C4)S(=O)(=O)C.C1=CC=C(C=C1)S(=O)(=O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00666926 Completed Drug: PF00562271 Head and Neck Neoplasm|Prostatic Neoplasm|Pancreatic Neoplasm Verastem Inc. December 2005 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    We are planning both in vitro and in vivo experiments and want to know how to reconstitute the drug for these purposes?

  • Answer:

    PF-00562271 has poor solubility in DMSO and water. Its solubility in DMSO is only 0.4mg/ml. In a previous literature report (http://www.ncbi.nlm.nih.gov/pubmed/18339875), the author used 5% Gelucire to formulate the compound. You can also consider other co-solvents such as PEG400, CMC, Tween80, and Captisol.

  • Question 2:

    Can you provide with a few common vehicles for PF-00562271, S2672 for use as oral gavage?

  • Answer:

    S2672 PF-00562271 can be dissolved in 0.5% CMC Na at 30 mg/ml as a suspension. If 4% DMSO can be used in your experiment, it will help dissolving the suspension more homogeneously.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID