PF-00562271 Besylate

For research use only. Not for use in humans.

Catalog No.S2672 Synonyms: PF-562271 Besylate

8 publications

PF-00562271 Besylate Chemical Structure

Molecular Weight(MW): 665.66

PF-00562271 Besylate is the benzenesulfonate salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.

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Selleck's PF-00562271 Besylate has been cited by 8 publications

4 Customer Reviews

  • Mol Ther 2012 20(5), 972-83. PF-00562271 Besylate purchased from Selleck.

    Pharmacological inhibition of optoFAK with the ATP-competitive FAK inhibitor PF-00562271. OptoFAK expressing SC4 cells were serum-starved for 24 h in the absence or presence of PF-00562271 (1 mM) before being illuminated for 10 min with blue light (451 nm, 2 μmol m-2 s-1) or kept in the dark.

    Cell Signal, 2018, 42:176-183. PF-00562271 Besylate purchased from Selleck.

  • The migration index enhanced by CX3CL1 was dramatic reduced using Bosutinib and PF-00562271. CX3CL1-only group as control. Scale bar = 200 μm. The experiments were repeated three times. *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001.

    J Cancer, 2018, 9(19):3603-3612. PF-00562271 Besylate purchased from Selleck.

    SGC-7901 and MGC-803 cells were treated with OLFM4-sh lentivirus or FAK inhibitor (PF) alone, or co-treated with OLFM4-sh lentivirus and PF. Cellular invasive ability was measured by transwell assay after indicated treatment. Data are expressed as mean ± standard deviation from three independent experiments. One was analysis of variance (ANOVA) with Bonferroni T post-test was used to analysis the data. *P < 0.05, ***P < 0.001; ##P < 0.01, ###P < 0.001 VS. OLFM4-sh group.

    BMB Rep, 2015, 48(11):630-5. PF-00562271 Besylate purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-00562271 Besylate is the benzenesulfonate salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 Besylate shows the selective inhibitory effects on FAK and Pyk2 tyrosine kinase activity with IC50 of 1.5 nM and 14 nM, respectively. And in cell-based assays, the IC50 of PF-562271 is shown to be 5 nM for FAK, which is more selective compared to other kinase targets. [1] In 2 dimensional (2D) cultures, PF-562271 results in a dose-dependent cell proliferation inhibition in FAK WT, FAK−/− and FAK kinase-deficient (KD) cells with IC50 of 3.3 μM, 2.08 μM and 2.01 μM, respectively. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 cell M{\5b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NITDXHBKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJ5Nk[g{txO NX24OYw4W0GQR1XS
human SW982 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUnmc5ZIUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m4NkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzJ6MjFOwG0> MUjTRW5ITVJ?
human KM12 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGi3bXHuJGtOOTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkO4OVU4KM7:TR?= MlrFV2FPT0WU
human COLO-205 cell NVXuXog3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn22TY5pcWKrdHnvckBw\iCqdX3hckBEV0yRLUKwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPDh4NUig{txO NELjcVhUSU6JRWK=
human COLO-829 cell M2rFZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4fNfWlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz24Nlkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd4MUe2JO69VQ>? NV24R25GW0GQR1XS
human MG-63 cell NHTyNXBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1vKcGlvcGmkaYTpc44hd2ZiaIXtZY4hVUdvNkOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlgxPjN5IN88US=> NWPEUVBKW0GQR1XS
human IGROV-1 cell NUPIRZBbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlHnTY5pcWKrdHnvckBw\iCqdX3hckBKT1KRVj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45OTB|ODFOwG0> NVz1bXJyW0GQR1XS
human NCI-H650 cell NXO3PWpMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYjDZY5SUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFY2OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOEOxOVQh|ryP NW\tXVNwW0GQR1XS
human RT-112 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUPtSZR7UW6qaXLpeIlwdiCxZjDoeY1idiCUVD2xNVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjl6NE[g{txO M1LQS3NCVkeHUh?=
human BCPAP cell Mm\xS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX7hWpBIUW6qaXLpeIlwdiCxZjDoeY1idiCEQ2DBVEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODF{OEig{txO M3r2NnNCVkeHUh?=
ALL-PO cell M3P3ZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M37iNmlvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4xOTV6NDFOwG0> MnTJV2FPT0WU
human KYSE-270 cell MkHUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXLwXlRXUW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVI4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMES3NVQh|ryP NYTj[Iw2W0GQR1XS
human 8305C cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUjJcohq[mm2aX;uJI9nKGi3bXHuJFg{ODWFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6wPVkxPCEQvF2= M4PRUHNCVkeHUh?=
NCI-H810 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUG0fXZHUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFgyOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMUC3O|Yh|ryP NWXRXHlUW0GQR1XS
human CAL-33 cell NUfOSlNXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3PveWlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUOzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4yOjl|ODFOwG0> NYX4Z45yW0GQR1XS
human AN3-CA cell NGLk[IRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXfnfmFZUW6qaXLpeIlwdiCxZjDoeY1idiCDTkOtR2Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJzOE[3JO69VQ>? MUPTRW5ITVJ?
human NKM-1 cell NUjlWVlOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEXhTFhKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zPzVyNjFOwG0> MVLTRW5ITVJ?
human BPH-1 cell MonrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHTScWRKdmirYnn0bY9vKG:oIHj1cYFvKEKSSD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zQDd4NjFOwG0> NX3U[JJlW0GQR1XS
human MES-SA cell M1\m[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXPJcohq[mm2aX;uJI9nKGi3bXHuJG1GWy2VQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|A3QDJizszN MVTTRW5ITVJ?
human CAL-62 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVfGbXl{UW6qaXLpeIlwdiCxZjDoeY1idiCFQVytOlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjNzOUC5JO69VQ>? NHrpSmJUSU6JRWK=
human KYSE-150 cell NWT6fmFmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPYNYt4UW6qaXLpeIlwdiCxZjDoeY1idiCNWWPFMVE2OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwM{WyN|Yh|ryP MVTTRW5ITVJ?
human SK-UT-1 cell MoPvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3Sw[GlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvVWStNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDR4NEeg{txO NWDxPFR[W0GQR1XS
human HUTU-80 cell NXHqdVRqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M37BSmlvcGmkaYTpc44hd2ZiaIXtZY4hUFWWVT24NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDR6OE[g{txO NFHR[pJUSU6JRWK=
human SIG-M5 cell NYTGcml2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHPHPJVKdmirYnn0bY9vKG:oIHj1cYFvKFOLRz3NOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDh2OEeg{txO NXHDOVRHW0GQR1XS
human AGS cell NGnJRoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUDhN2J7UW6qaXLpeIlwdiCxZjDoeY1idiCDR2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlUzOTJ2IN88US=> M{\F[3NCVkeHUh?=
human ST486 cell NIiwT|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NU\LZllZUW6qaXLpeIlwdiCxZjDoeY1idiCVVES4OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTN{N{ig{txO NHe3UWVUSU6JRWK=
human HSC-2 cell NGHWbIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmLLTY5pcWKrdHnvckBw\iCqdX3hckBJW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVM6PSEQvF2= MWXTRW5ITVJ?
human BC-1 cell MnXPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NInRSnBKdmirYnn0bY9vKG:oIHj1cYFvKEKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlYyPjZ2IN88US=> NXnJXJlKW0GQR1XS
human CGTH-W-1 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYfURYdJUW6qaXLpeIlwdiCxZjDoeY1idiCFR2TIMXcuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwNkG2O|kh|ryP NY\KfWVnW0GQR1XS
human MZ1-PC cell MorkS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEnKVJRKdmirYnn0bY9vKG:oIHj1cYFvKE2cMT3QR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjJ|MUKg{txO MXTTRW5ITVJ?
human SW1710 cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmjoTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlYzPjJ6IN88US=> NU\NdoFPW0GQR1XS
human EW-13 cell NIPh[WVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn3pTY5pcWKrdHnvckBw\iCqdX3hckBGXy1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlM1PjZizszN NVTFOnRYW0GQR1XS
human U251 cell M2PZSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlvSTY5pcWKrdHnvckBw\iCqdX3hckBWOjVzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63OFA{OSEQvF2= NELje|ZUSU6JRWK=
human NCI-H460 cell MkXxS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1TqfmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi0OlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB2OEO5JO69VQ>? NXTodIZmW0GQR1XS
human DU-4475 cell M2\VfWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1fSe2lvcGmkaYTpc44hd2ZiaIXtZY4hTFVvNES3OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvOTR5NUmg{txO MmjPV2FPT0WU
human MFE-296 cell NEPuVIFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4nHfmlvcGmkaYTpc44hd2ZiaIXtZY4hVU[HLUK5OkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPDd5OUKg{txO MorBV2FPT0WU
human DU-145 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGLXfplKdmirYnn0bY9vKG:oIHj1cYFvKESXLUG0OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPDlzMUig{txO NGS2VWdUSU6JRWK=
human MDA-MB-231 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXPtVWw5UW6qaXLpeIlwdiCxZjDoeY1idiCPRFGtUWIuOjNzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mj60PVU4OiEQvF2= NU\O[nF1W0GQR1XS
human SNU-387 cell M2q0cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXO0RY5uUW6qaXLpeIlwdiCxZjDoeY1idiCVTmWtN|g4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi53MkiyJO69VQ>? MXLTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo In several human s.c. xenograft models, PF-562271 exhibits dose-dependent tumor growth inhibition, and produces maximum tumor inhibition for PC-3M, BT474, BxPc3, and LoVo ranging from 78% to 94% inhibition at doses of 25 to 50 mg/kg twice daily, without weight loss, morbidity, or death. [1] PF-562271 (25 mg/kg by p.o.) leads to a significant decrease in tumor progression in both subcutaneous and bone metastasis PC3M-luc-C6 xenograft models. [3] In a Huh7.5 hepatocellular carcinoma xenograft model, combination therapy of sunitinib and PF-562271 targets angiogenesis and tumor aggressiveness, and produces more significant anti-tumor effect than single agent by blocking tumor growth and impacting the ability of the tumor to recover upon withdrawal of the therapy. [4]

Protocol

Kinase Assay:

[1]

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Recombinant kinase assay and enzyme kinetics :

Briefly, purified-activated FAK kinase domain (amino acid 410–689) is reacted with 50 μM ATP and 10 μg per well of a random peptide polymer of Glu and Tyr, p(Glu/Tyr), in kinase buffer [50 mM HEPES (pH 7.5), 125 mM NaCl, and 48 mM MgCl2] for 15 minutes. Phosphorylation of p(Glu/Tyr) is challenged with serially diluted PF-562271 at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is tested in triplicate. Phosphorylation of p(Glu/Tyr) is detected with a general antiphospho-tyrosine (PY20) antibody followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody. HRP substrate is added, and absorbance readings at 450 nm are obtained after addition of stop solution (2 M H2SO4). IC50 values are determined using the Hill-Slope Model. Broad kinase selectivity profiling is performed in house and by using the KinaseProfiler Selectivity Screening Service available through UpState Biotechnology.
Cell Research:

[2]

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  • Cell lines: Squamous cell carcinoma (SCC)
  • Concentrations: 0 to 1 μM
  • Incubation Time: 72 hours
  • Method:

    Cells are plated for 48 hours before addition of PF-562271. After 3 days cells are fixed by addition of ice cold 25% trichloroacetic acid (TCA) solution prior to staining with Sulforhodamine B (SRB) dye solution. Plates are washed with 1% glacial acetic acid, air-dried and resuspended in 10 mM Tris buffer, pH 10.5 before reading absorbance at 540 nm. Curve fitting and generation of IC50 values is carried out using GraphPad Prism 4 software from six replicates.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: PC-3M, BT474, BxPc3, LoVo, U87MG, H125 and H460 cells are injected s.c. into the right flank of athymic female mice .
  • Formulation: PF-562271 is dissolved in 5% Gelucire.
  • Dosages: ≤100 mg/kg
  • Administration: Administered via p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL warmed (21.03 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
3mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 665.66
Formula

C21H20F3N7O3S.C6H6O3S

CAS No. 939791-38-5
Storage powder
in solvent
Synonyms PF-562271 Besylate
Smiles CN(C1=C(CNC2=C(C=NC(=N2)NC3=CC=C4NC(=O)CC4=C3)C(F)(F)F)C=CC=N1)[S](C)(=O)=O.O[S](=O)(=O)C5=CC=CC=C5

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    We are planning both in vitro and in vivo experiments and want to know how to reconstitute the drug for these purposes?

  • Answer:

    PF-00562271 has poor solubility in DMSO and water. Its solubility in DMSO is only 0.4mg/ml. In a previous literature report (http://www.ncbi.nlm.nih.gov/pubmed/18339875), the author used 5% Gelucire to formulate the compound. You can also consider other co-solvents such as PEG400, CMC, Tween80, and Captisol.

  • Question 2:

    Can you provide with a few common vehicles for PF-00562271, S2672 for use as oral gavage?

  • Answer:

    S2672 PF-00562271 can be dissolved in 0.5% CMC Na at 30 mg/ml as a suspension. If 4% DMSO can be used in your experiment, it will help dissolving the suspension more homogeneously.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID