PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
USD 170 In stock
USD 270 In stock
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6 Customer Reviews

  • Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

    Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

    (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

  • D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

    Acta Pharmacol Sin, 2018, doi:10.1038/aps.2017.189. PF-562271 purchased from Selleck.

  • VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

    OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVP0e4NiUUN3ME2wMlI4PjZizszN NGTic5BUSU6JRWK=
SW982 NXzpbZRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwM{K4NkDPxE1? NFuxN4RUSU6JRWK=
KM12 NIT2N2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHCPZRKSzVyPUCuN|g2PTdizszN M2riPXNCVkeHUh?=
COLO-205 NEnqe21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnsVFY2UUN3ME2wMlQ5PjV6IN88US=> NV7XS3FyW0GQR1XS
COLO-829 NGm3[4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnZRpdSUUN3ME2wMlc2OTd4IN88US=> NIXCb3RUSU6JRWK=
MG-63 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDaTWM2OD1yLkiwOlM4KM7:TR?= M4XvUHNCVkeHUh?=
IGROV-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPzPWJFUUN3ME2wMlgyODN6IN88US=> M{\wTHNCVkeHUh?=
NCI-H650 NYPkOmo6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWe3Z5FKUUN3ME2wMlg{OTV2IN88US=> M1nMOHNCVkeHUh?=
RT-112 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTMcm9KSzVyPUCuPVg1PiEQvF2= NXfBfoZJW0GQR1XS
BCPAP NGLsS5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3tTWM2OD1zLkCxNlg5KM7:TR?= NX\ZS2h3W0GQR1XS
ALL-PO MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX22U3Q1UUN3ME2xMlAyPTh2IN88US=> NI\ST|NUSU6JRWK=
KYSE-270 NUWzZWJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\MTWM2OD1zLkC0O|E1KM7:TR?= NH;vVHRUSU6JRWK=
8305C M1e4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTSfpV7UUN3ME2xMlA6QTB2IN88US=> NXy0TlFSW0GQR1XS
NCI-H810 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfxTWM2OD1zLkGwO|c3KM7:TR?= MknvV2FPT0WU
CAL-33 MnjRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4H3d2lEPTB;MT6xNlk{QCEQvF2= MWXTRW5ITVJ?
AN3-CA MljaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LR[WlEPTB;MT6yNVg3PyEQvF2= Ml3PV2FPT0WU
NKM-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;zTWM2OD1zLkK3OVA3KM7:TR?= MlfmV2FPT0WU
BPH-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTFwMki3OlYh|ryP M{THenNCVkeHUh?=
MES-SA MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTFwM{C2PFIh|ryP NEnZSXJUSU6JRWK=
CAL-62 MmLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfPeZlKSzVyPUGuN|E6ODlizszN MXTTRW5ITVJ?
Detroit562 NX64bJRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTFwM{WyNlIh|ryP NXzVd3F7W0GQR1XS
KYSE-150 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37jPWlEPTB;MT6zOVI{PiEQvF2= NV;4XnpKW0GQR1XS
SK-UT-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDRfHZKSzVyPUGuOFQ3PDdizszN NIHlSllUSU6JRWK=
HUTU-80 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjBelNKSzVyPUGuOFQ5QDZizszN NVfv[VZEW0GQR1XS
SIG-M5 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DPTWlEPTB;MT60PFM5PyEQvF2= NIjob29USU6JRWK=
AGS NXXMeYtrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUezcFlPUUN3ME2xMlUzOTJ2IN88US=> NXXCVWhDW0GQR1XS
ST486 NXfDeG0zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzQd2FKSzVyPUGuOVMzPzhizszN M4rzenNCVkeHUh?=
HSC-2 NYDj[IFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTFwNUO5OUDPxE1? M{Tzc3NCVkeHUh?=
LCLC-103H NVHrXW5kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HSRWlEPTB;MT61OVgyQCEQvF2= Mlv0V2FPT0WU
BC-1 NGOyS2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHvTWM2OD1zLk[xOlY1KM7:TR?= MVrTRW5ITVJ?
CGTH-W-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;lRW9KSzVyPUGuOlE3PzlizszN MmX0V2FPT0WU
MZ1-PC MnT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPDXZJKSzVyPUGuOlI{OTJizszN MoTZV2FPT0WU
SW1710 MmfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2qwPGlEPTB;MT62NlYzQCEQvF2= MULTRW5ITVJ?
EW-13 NVPjT4wzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTFwNkO0OlYh|ryP NYq5bYd3W0GQR1XS
TE-11 NEnpeVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M362V2lEPTB;MT62N|UzOiEQvF2= MmDRV2FPT0WU
BHY MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHWx[GRKSzVyPUGuOlU3PjNizszN M3zTcHNCVkeHUh?=
ML-2 NEnFZ3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofITWM2OD1zLk[2PFAzKM7:TR?= Mon6V2FPT0WU
MEL-JUSO NWXue5N6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HoVGlEPTB;MT63Nlg6OSEQvF2= Ml:zV2FPT0WU
U251 Ml[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTzTWM2OD1zLke0NFMyKM7:TR?= NHPyeGFUSU6JRWK=
MHH-ES-1 NHjC[IlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTFwN{m1Olgh|ryP M2nTVnNCVkeHUh?=
CAL-85-1 M1;oZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrXRm9WUUN3ME2xMlgxPDlizszN NG\LVoZUSU6JRWK=
D-336MG M3y1eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2X0NmlEPTB;MT64OVYyPCEQvF2= MXzTRW5ITVJ?
VA-ES-BJ NEXoTFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHlTY1TUUN3ME2xMlkxOzF3IN88US=> M3noOHNCVkeHUh?=
MDA-MB-468 NFfUVW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY[3[lA4UUN3ME2xMlkyOjd{IN88US=> NUXTWJI{W0GQR1XS
MEL-HO NVHvT4JbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTFwOUe5PVMh|ryP M1Hvc3NCVkeHUh?=
SW954 NVvFZopHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXUOYNKSzVyPUKuNFAyPTFizszN MWfTRW5ITVJ?
LB831-BLC MoqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLNTWM2OD1{LkCwPVg5KM7:TR?= MUjTRW5ITVJ?
EW-16 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\VcWlEPTB;Mj6wN|k5OyEQvF2= MYXTRW5ITVJ?
NCI-H460 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTJwMES4N|kh|ryP NXvNSoJFW0GQR1XS
CHP-212 NFzxXJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUi2eYd1UUN3ME2yMlA6PTN7IN88US=> NFu4UlZUSU6JRWK=
MHH-NB-11 M2WzZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\xTWM2OD1{LkGyPFE5KM7:TR?= MoTrV2FPT0WU
DU-4475 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnETWM2OD1{LkG0O|U6KM7:TR?= M2fjTnNCVkeHUh?=
ABC-1 NW\QXWZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjySm1KSzVyPUKuNlUyQTZizszN MoiwV2FPT0WU
LOUCY MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrRdnhKSzVyPUKuNlYyOjhizszN Mlf0V2FPT0WU
LC-2-ad M2TJdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrRXJVKSzVyPUKuNlY6PjZizszN MmjCV2FPT0WU
ETK-1 NYXQRWpNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETyXI5KSzVyPUKuNlg1PiEQvF2= NVu0Rm9qW0GQR1XS
KYSE-180 M4PuNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NImxdG5KSzVyPUKuNlg5PzRizszN NH75OWxUSU6JRWK=
HOP-92 NVvOc|huT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;SRnVXUUN3ME2yMlMxPjd|IN88US=> MoTqV2FPT0WU
RCC10RGB NIDpdItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonaTWM2OD1{LkOyOVA4KM7:TR?= NXz4VXVCW0GQR1XS
KASUMI-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITDW3pKSzVyPUKuN|I5QCEQvF2= M2XzdnNCVkeHUh?=
ACN NWHCW|FtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M131ZmlEPTB;Mj6zOFc1PSEQvF2= NEXxUopUSU6JRWK=
BxPC-3 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPsTWM2OD1{LkO1OFM3KM7:TR?= NEj5XGlUSU6JRWK=
MONO-MAC-6 NILSNpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjnTXBKSzVyPUKuOFEzPDhizszN Ml3jV2FPT0WU
CAL-39 MkC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17zZmlEPTB;Mj60NVYzQCEQvF2= M{TuSnNCVkeHUh?=
CMK NWfFNnlHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPFfnBKSzVyPUKuOFE4QTVizszN Mn;ZV2FPT0WU
MFE-296 M2TnXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HTNGlEPTB;Mj60O|c6OiEQvF2= NIHIXYJUSU6JRWK=
DU-145 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfvV4dKSzVyPUKuOFkyOThizszN MXXTRW5ITVJ?
MDA-MB-231 MlzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHz3cJJKSzVyPUKuOFk2PzJizszN NUL3SotyW0GQR1XS
EFO-27 MmnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\sSmlEPTB;Mj61NVg5QCEQvF2= M{fq[XNCVkeHUh?=
SNU-387 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGKxc3JKSzVyPUKuOVI5OiEQvF2= NWXCXplrW0GQR1XS
KYSE-410 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PxbWlEPTB;Mj61OlUyPyEQvF2= MX7TRW5ITVJ?
NCCIT NH2yN4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3GTWM2OD1{LkW3NlQh|ryP MkLoV2FPT0WU
LXF-289 M{TW[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTJwNUiyO|Mh|ryP M3zFVnNCVkeHUh?=
HMV-II NIOwV5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTpfIlKSzVyPUKuOVg6QTdizszN NWnuUZZ1W0GQR1XS
MOLT-4 NGHY[nNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTJwNkC3NVIh|ryP NUXzRVZjW0GQR1XS
KOSC-2 Ml3QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPjZ5pKSzVyPUKuOlM5PzlizszN MlXzV2FPT0WU
TUR NWXUeZhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzUUFBKSzVyPUKuOlU{PjlizszN MnPvV2FPT0WU
NCI-H2087 M1jlRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTJwN{G2OFYh|ryP MXTTRW5ITVJ?
SCC-15 MlH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rW[2lEPTB;Mj63OVI2OiEQvF2= MXLTRW5ITVJ?
HSC-4 NWrFWFl4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTJwN{izPFkh|ryP NIXwU5hUSU6JRWK=
G-402 MnziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjIbopKSzVyPUKuPFE1PDdizszN MkCyV2FPT0WU
GP5d MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHWW2hKSzVyPUKuPFM5OjdizszN NVG0NIVvW0GQR1XS
IGR-1 NYfmZndnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXJclVDUUN3ME2yMlg{QTh5IN88US=> M1rzOnNCVkeHUh?=
SK-PN-DW MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDYR2p2UUN3ME2yMlg3OjB2IN88US=> NETNclBUSU6JRWK=
OPM-2 MmfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jwN2lEPTB;Mj64PVk4QCEQvF2= NGPm[4VUSU6JRWK=
5637 NWrubHhlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfMbFh[UUN3ME2yMlkzPDN7IN88US=> MVLTRW5ITVJ?
NCI-H2030 NHvr[WdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTJwOUiwNlkh|ryP NUPrXZdZW0GQR1XS
SK-LU-1 MoLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUG3cGpCUUN3ME2yMlk5OzN2IN88US=> NYnCPGlDW0GQR1XS
EW-18 NFHjWmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHITWM2OD1|LkC1N|Ih|ryP M{ntdnNCVkeHUh?=
786-0 M1r2RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmX3TWM2OD1|LkC1O|g1KM7:TR?= Mk\yV2FPT0WU
NCI-H1963 M2\ES2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zuRWlEPTB;Mz6wPFgzPSEQvF2= MnfzV2FPT0WU
A2780 MoTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVToNXdIUUN3ME2zMlExOzRzIN88US=> M{jsPHNCVkeHUh?=
NCI-H526 M{[0c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnkZXlbUUN3ME2zMlE{QTZzIN88US=> NVezRW86W0GQR1XS
BHT-101 Mk\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\sVGlEPTB;Mz6xOVc6QCEQvF2= Mk[0V2FPT0WU
RPMI-7951 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTNwMkCxN|ch|ryP MVrTRW5ITVJ?
HL-60 MknuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzSW4ZKSzVyPUOuNlEyPCEQvF2= M2HDcXNCVkeHUh?=
CTV-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLWVZdKSzVyPUOuNlU{PzdizszN MoLqV2FPT0WU
IST-SL1 M13lXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{L1R2lEPTB;Mz6yPVQ3PSEQvF2= NEDhU3lUSU6JRWK=
G-401 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjiWndnUUN3ME2zMlI6QDB6IN88US=> MkniV2FPT0WU
OVCAR-5 NWD5RnlLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTJdGNKSzVyPUOuN|Y{PTJizszN M3\p[XNCVkeHUh?=
EW-1 NIHpSnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHv4SYVKSzVyPUOuOFA2PDRizszN NUnOfYx5W0GQR1XS
H4 MoXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFOz[pRKSzVyPUOuOFc2PjlizszN M2rnZXNCVkeHUh?=
EM-2 NWjOXWhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLuVnhKSzVyPUOuOFg3PzRizszN MXrTRW5ITVJ?
RS4-11 NWfvS2Y6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTWXGFKSzVyPUOuOFg4OTVizszN NWizd|FUW0GQR1XS
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NCI-H2170 NYHtfYtsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\ZTWM2OD1|OT65PVc4KM7:TR?= M1LLbnNCVkeHUh?=
COLO-678 M4rLWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUD5OGtHUUN3ME20NE42QTVizszN M2DEbXNCVkeHUh?=
PF-382 NIXnZZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHSwTHVKSzVyPUSwMlc1QDdizszN MnnDV2FPT0WU
OAW-42 NXnRWoxiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rFRmlEPTB;NEKuNlk1OiEQvF2= M2HrZXNCVkeHUh?=
LB996-RCC M3rEOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG[xZ4JKSzVyPUSyMlgxODhizszN NITRUIdUSU6JRWK=
SAS M{jsbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTR|LkSyPFgh|ryP NUHFfpVCW0GQR1XS
MRK-nu-1 M3KzdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLNVYNJUUN3ME20OE44PDF2IN88US=> NVHrOoNGW0GQR1XS
EoL-1-cell MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4q5eGlEPTB;NESuPVE{PiEQvF2= M1X3UHNCVkeHUh?=
NCI-H2342 NF7nW5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEL1e5VKSzVyPUS1MlEyOTdizszN MnrwV2FPT0WU
NCI-H1703 MmPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXKTmtRUUN3ME20Ok4xPTl{IN88US=> NVTMWYtSW0GQR1XS
NCI-H187 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTR4LkG4Olkh|ryP MXjTRW5ITVJ?
CP66-MEL NYniUVZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPyWZZKSzVyPUS2MlI4QDlizszN NFWwUnNUSU6JRWK=
HCC1569 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zOSWlEPTB;NE[uOVkyPyEQvF2= MUXTRW5ITVJ?
BL-70 M4Tm[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonZTWM2OD12Nj63O|M4KM7:TR?= M1rQWXNCVkeHUh?=
Saos-2 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTR5LkOxNlMh|ryP NYP3RZJJW0GQR1XS
LC-1F NYC4cI9VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PPPGlEPTB;NEeuOlExPSEQvF2= MnTKV2FPT0WU
KY821 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlX6TWM2OD12Nz62OFM1KM7:TR?= MXXTRW5ITVJ?
PSN1 NIjPOpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTR6LkG0NFEh|ryP Ml7zV2FPT0WU
RT4 NEfXZ5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvoZmZ7UUN3ME20PE45Ozd5IN88US=> MofDV2FPT0WU
LS-1034 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TuV2lEPTB;NEiuPFk2PCEQvF2= MXPTRW5ITVJ?
NCI-H1395 NGDVWHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTR7LkC0PVIh|ryP M2m0SXNCVkeHUh?=
EKVX NIGx[ZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnT0TWM2OD12OT62O|MyKM7:TR?= Mn7HV2FPT0WU
IST-MES1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnezTWM2OD12OT64PVQ1KM7:TR?= NILSZ3lUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

Related FAK Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID