PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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USD 270 In stock
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Cited by 14 Publications

6 Customer Reviews

  • Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

    Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

    (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

  • D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

    Acta Pharmacol Sin, 2018, doi:10.1038/aps.2017.189. PF-562271 purchased from Selleck.

  • VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

    OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXmyS5lpUUN3ME2wMlI4PjZizszN NFP1VmZUSU6JRWK=
SW982 MkPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwM{K4NkDPxE1? M{j1U3NCVkeHUh?=
KM12 NX72N|Q{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLhW29LUUN3ME2wMlM5PTV5IN88US=> NVzvT3ZOW0GQR1XS
COLO-205 Ml7TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfwTWM2OD1yLkS4OlU5KM7:TR?= MUXTRW5ITVJ?
COLO-829 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvPTWM2OD1yLke1NVc3KM7:TR?= NI\YOJdUSU6JRWK=
MG-63 NHTSZm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTBwOEC2N|ch|ryP MWTTRW5ITVJ?
IGROV-1 NEf5SFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfETVRKSzVyPUCuPFExOzhizszN NUTCWHJWW0GQR1XS
NCI-H650 NFPreGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C0e2lEPTB;MD64N|E2PCEQvF2= MnHXV2FPT0WU
RT-112 NFzwVGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwOUi0OkDPxE1? NF;NSXBUSU6JRWK=
BCPAP NH7HN|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DQZ2lEPTB;MT6wNVI5QCEQvF2= MljZV2FPT0WU
ALL-PO M2L0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zySGlEPTB;MT6wNVU5PCEQvF2= NHjrZnVUSU6JRWK=
KYSE-270 M4i3XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHZTWM2OD1zLkC0O|E1KM7:TR?= NGDhNWxUSU6JRWK=
8305C NIDEXG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDZTJJPUUN3ME2xMlA6QTB2IN88US=> NYDpRY1bW0GQR1XS
NCI-H810 M2XBNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUS0SnprUUN3ME2xMlExPzd4IN88US=> NGL2RXFUSU6JRWK=
CAL-33 NVnhVnltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTFwMUK5N|gh|ryP NYfIXW1wW0GQR1XS
AN3-CA Mlq5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTFwMkG4Olch|ryP MonhV2FPT0WU
NKM-1 NV\kV4dST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\xTXVkUUN3ME2xMlI4PTB4IN88US=> NEjCflJUSU6JRWK=
BPH-1 NEO0WWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPs[FBPUUN3ME2xMlI5PzZ4IN88US=> NWnwTmVsW0GQR1XS
MES-SA M{fvW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTFwM{C2PFIh|ryP NFrPUo5USU6JRWK=
CAL-62 M2jpU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTFwM{G5NFkh|ryP M1;WRnNCVkeHUh?=
Detroit562 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLEZmVKSzVyPUGuN|UzOjJizszN NYf4[YF7W0GQR1XS
KYSE-150 MlHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3W5PWlEPTB;MT6zOVI{PiEQvF2= M3r0UHNCVkeHUh?=
SK-UT-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTQTWM2OD1zLkS0OlQ4KM7:TR?= NEjy[HRUSU6JRWK=
HUTU-80 M1jTdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13xTGlEPTB;MT60OFg5PiEQvF2= Mon3V2FPT0WU
SIG-M5 NWTJOIM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTSWGNvUUN3ME2xMlQ5Ozh5IN88US=> MWXTRW5ITVJ?
AGS NUfK[og6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkG3TWM2OD1zLkWyNVI1KM7:TR?= NUfUfXd[W0GQR1XS
ST486 NU\idIt1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITHSJNKSzVyPUGuOVMzPzhizszN MlH0V2FPT0WU
HSC-2 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPXbWlpUUN3ME2xMlU{QTVizszN NXnONppuW0GQR1XS
LCLC-103H NVr3eWNQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLXOoJRUUN3ME2xMlU2QDF6IN88US=> M3ezbXNCVkeHUh?=
BC-1 NH3DenpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7vfZdKSzVyPUGuOlE3PjRizszN NEDRdFFUSU6JRWK=
CGTH-W-1 Ml73S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PBc2lEPTB;MT62NVY4QSEQvF2= NI\oRXFUSU6JRWK=
MZ1-PC M1fLVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVm2boJEUUN3ME2xMlYzOzF{IN88US=> NYfHcWFHW0GQR1XS
SW1710 Mm\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTFwNkK2Nlgh|ryP MV3TRW5ITVJ?
EW-13 NGGwOoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlH0TWM2OD1zLk[zOFY3KM7:TR?= NUjofYFLW0GQR1XS
TE-11 MlvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvNTWM2OD1zLk[zOVIzKM7:TR?= NWHHeIlDW0GQR1XS
BHY M3\Xemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfydoU{UUN3ME2xMlY2PjZ|IN88US=> NEnucmdUSU6JRWK=
ML-2 MlzQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnL0TWM2OD1zLk[2PFAzKM7:TR?= MWXTRW5ITVJ?
MEL-JUSO M3y2U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLmTWM2OD1zLkeyPFkyKM7:TR?= M3rwenNCVkeHUh?=
U251 NFj1OI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jDVmlEPTB;MT63OFA{OSEQvF2= NVvZSm1nW0GQR1XS
MHH-ES-1 NXjXdWFnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXtb2JNUUN3ME2xMlc6PTZ6IN88US=> M{jxTnNCVkeHUh?=
CAL-85-1 MmT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTFwOEC0PUDPxE1? NF[4bVBUSU6JRWK=
D-336MG M37ES2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTFwOEW2NVQh|ryP M2XlVHNCVkeHUh?=
VA-ES-BJ M3TKeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkS2TWM2OD1zLkmwN|E2KM7:TR?= Mlf0V2FPT0WU
MDA-MB-468 NVfzTo84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{TRWGlEPTB;MT65NVI4OiEQvF2= MmXGV2FPT0WU
MEL-HO M{PXUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mln4TWM2OD1zLkm3PVk{KM7:TR?= NFLJelVUSU6JRWK=
SW954 NYjQdlZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTJwMECxOVEh|ryP MXnTRW5ITVJ?
LB831-BLC MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LGbWlEPTB;Mj6wNFk5QCEQvF2= NW\3VppzW0GQR1XS
EW-16 MlqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rQXWlEPTB;Mj6wN|k5OyEQvF2= NVvaemRsW0GQR1XS
NCI-H460 M4jlcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTJwMES4N|kh|ryP MXnTRW5ITVJ?
CHP-212 MnfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjOTWM2OD1{LkC5OVM6KM7:TR?= M1\lS3NCVkeHUh?=
MHH-NB-11 M3G4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2n6fmlEPTB;Mj6xNlgyQCEQvF2= NHTyRmhUSU6JRWK=
DU-4475 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzOTWM2OD1{LkG0O|U6KM7:TR?= NVK0Rmc{W0GQR1XS
ABC-1 NYPRcZN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnuyTWM2OD1{LkK1NVk3KM7:TR?= MWjTRW5ITVJ?
LOUCY MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEizTlVKSzVyPUKuNlYyOjhizszN NULSNmlWW0GQR1XS
LC-2-ad M3fnTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF34[lVKSzVyPUKuNlY6PjZizszN NUOyRWJrW0GQR1XS
ETK-1 NWjiWWY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\jOmlEPTB;Mj6yPFQ3KM7:TR?= NYHZbpIyW0GQR1XS
KYSE-180 NUXueWZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnOyTWM2OD1{LkK4PFc1KM7:TR?= NYr2Znl1W0GQR1XS
HOP-92 MnrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{GySGlEPTB;Mj6zNFY4OyEQvF2= MnLvV2FPT0WU
RCC10RGB MljqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrKTWM2OD1{LkOyOVA4KM7:TR?= MkXwV2FPT0WU
KASUMI-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnpdGRKSzVyPUKuN|I5QCEQvF2= Mnq0V2FPT0WU
ACN MlKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTJwM{S3OFUh|ryP NYfwV3ppW0GQR1XS
BxPC-3 NXvseVhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHu0dVVKSzVyPUKuN|U1OzZizszN NXnYblY5W0GQR1XS
MONO-MAC-6 NEPwb2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\xbGlEPTB;Mj60NVI1QCEQvF2= NF3oeYhUSU6JRWK=
CAL-39 NYC2d2lyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml2wTWM2OD1{LkSxOlI5KM7:TR?= MmHSV2FPT0WU
CMK NIjZXZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTJwNEG3PVUh|ryP M4jodnNCVkeHUh?=
MFE-296 MnW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLVcFVxUUN3ME2yMlQ4Pzl{IN88US=> M3zHNHNCVkeHUh?=
DU-145 NI\WeGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoL6TWM2OD1{LkS5NVE5KM7:TR?= M2\BVXNCVkeHUh?=
MDA-MB-231 NF30SIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTJwNEm1O|Ih|ryP MYXTRW5ITVJ?
EFO-27 NVLMVZFrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGG4folKSzVyPUKuOVE5QDhizszN M3K2SHNCVkeHUh?=
SNU-387 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTafIZ1UUN3ME2yMlUzQDJizszN MnjNV2FPT0WU
KYSE-410 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHaT493UUN3ME2yMlU3PTF5IN88US=> MXfTRW5ITVJ?
NCCIT NYTLUWNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzGTVJKSzVyPUKuOVczPCEQvF2= Mlu1V2FPT0WU
LXF-289 NFP0XZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHQTWM2OD1{LkW4Nlc{KM7:TR?= NXrCT3o5W0GQR1XS
HMV-II M3jp[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIr6SnFKSzVyPUKuOVg6QTdizszN M4H5OnNCVkeHUh?=
MOLT-4 NFvoT2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIToflFKSzVyPUKuOlA4OTJizszN MWTTRW5ITVJ?
KOSC-2 NYTVdmpnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LxfmlEPTB;Mj62N|g4QSEQvF2= NXPvPIpHW0GQR1XS
TUR M3GwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zoOWlEPTB;Mj62OVM3QSEQvF2= NW\5doJkW0GQR1XS
NCI-H2087 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDMTXFKSzVyPUKuO|E3PDZizszN NUDF[HRJW0GQR1XS
SCC-15 NX7LXm1PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFX3XI9KSzVyPUKuO|UzPTJizszN M1q2cXNCVkeHUh?=
HSC-4 NX;6PJVpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XGe2lEPTB;Mj63PFM5QSEQvF2= MW\TRW5ITVJ?
G-402 NXrTe2ZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHTbVBnUUN3ME2yMlgyPDR5IN88US=> NXW5SI9WW0GQR1XS
GP5d MofBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXKXWFWUUN3ME2yMlg{QDJ5IN88US=> MWTTRW5ITVJ?
IGR-1 M1nvdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33VfGlEPTB;Mj64N|k5PyEQvF2= NIC2SFRUSU6JRWK=
SK-PN-DW MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLJTWM2OD1{Lki2NlA1KM7:TR?= NXfre2J6W0GQR1XS
OPM-2 MofHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HHSGlEPTB;Mj64PVk4QCEQvF2= NHjKNJpUSU6JRWK=
5637 NYPrZ3QxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHtTWM2OD1{LkmyOFM6KM7:TR?= M1q0UXNCVkeHUh?=
NCI-H2030 NXjkTVFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTJwOUiwNlkh|ryP NYrLNJF7W0GQR1XS
SK-LU-1 NIniW3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37UZmlEPTB;Mj65PFM{PCEQvF2= NYH5U2t[W0GQR1XS
EW-18 NXnIbphqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG[0RZBKSzVyPUOuNFU{OiEQvF2= NUTBSnBFW0GQR1XS
786-0 NUDuNoxTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTNwMEW3PFQh|ryP MnLEV2FPT0WU
NCI-H1963 NYTXOVNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TxU2lEPTB;Mz6wPFgzPSEQvF2= NES3UJpUSU6JRWK=
A2780 M1;DbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLBTWM2OD1|LkGwN|QyKM7:TR?= NGS2eWZUSU6JRWK=
NCI-H526 M{jPUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LpeGlEPTB;Mz6xN|k3OSEQvF2= NIrBc2NUSU6JRWK=
BHT-101 NYnz[2VMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnz3TWM2OD1|LkG1O|k5KM7:TR?= MmrPV2FPT0WU
RPMI-7951 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPnUVRjUUN3ME2zMlIxOTN5IN88US=> NVzpO4xGW0GQR1XS
HL-60 M1nzR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;MWVhKSzVyPUOuNlEyPCEQvF2= M1jyWnNCVkeHUh?=
CTV-1 MnzrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYW2VFFEUUN3ME2zMlI2Ozd5IN88US=> NF65U2VUSU6JRWK=
IST-SL1 MoG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkK5TWM2OD1|LkK5OFY2KM7:TR?= Mn64V2FPT0WU
G-401 M2GyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTiZWY5UUN3ME2zMlI6QDB6IN88US=> NGnndVBUSU6JRWK=
OVCAR-5 NF\aW|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFOybIFKSzVyPUOuN|Y{PTJizszN M{LlNnNCVkeHUh?=
EW-1 M1XvWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTNwNEC1OFQh|ryP M{nTWHNCVkeHUh?=
H4 NXq4e2R{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLKfZFiUUN3ME2zMlQ4PTZ7IN88US=> MV;TRW5ITVJ?
EM-2 MkjZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nqc2lEPTB;Mz60PFY4PCEQvF2= NUTKNGlTW0GQR1XS
RS4-11 NFuz[Y9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjHTWM2OD1|LkS4O|E2KM7:TR?= NHnCV2VUSU6JRWK=
NOMO-1 M3flWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\teWlEPTB;Mz60PVIxPCEQvF2= NF3KNZlUSU6JRWK=
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HT-1376 NIXiUYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfa[2w{UUN3ME2xPU4zODJ7IN88US=> MoDMV2FPT0WU
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NOS-1 M1;QS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37kb2lEPTB;MkCuNVU4PyEQvF2= M4nwWnNCVkeHUh?=
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LB2518-MEL MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLub3Y3UUN3ME2yNE4{QTV|IN88US=> MYDTRW5ITVJ?
PANC-10-05 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rYbGlEPTB;MkCuOFYyOyEQvF2= Mm\vV2FPT0WU
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OCUB-M MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\TXmlEPTB;MkGuPVY5QCEQvF2= M4fFTXNCVkeHUh?=
NCI-H1694 MojGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\yU4RvUUN3ME2yNU46QDZ|IN88US=> NY\TPIlHW0GQR1XS
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SKG-IIIa NV;wb5VIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TIPGlEPTB;MkOuN|U1PiEQvF2= M4PybnNCVkeHUh?=
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PC-14 M17oUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXIPIdtUUN3ME2yN{43PjJ|IN88US=> NVvCWW1mW0GQR1XS
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SW1990 NIjM[3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrlUml2UUN3ME2yOE42QDh|IN88US=> MmHXV2FPT0WU
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MDA-MB-175-VII MmO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfDdZVwUUN3ME2yO{43QTd3IN88US=> M4DPR3NCVkeHUh?=
HuP-T3 NVrtW25IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXr3fHREUUN3ME2yPE4xPDl3IN88US=> NGSzO5hUSU6JRWK=
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HDLM-2 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfXUXJOUUN3ME2yPE4zPDV7IN88US=> M3PYWXNCVkeHUh?=
NCI-H1651 M2\nPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzjTWM2OD1{OD6yOVkzKM7:TR?= NWD4UJQyW0GQR1XS
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NY NV7yUXZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnXdlg5UUN3ME2yPE43PTd7IN88US=> MYnTRW5ITVJ?
D-283MED MljNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVmw[Jg1UUN3ME2yPE45PzB6IN88US=> NHzmR2hUSU6JRWK=
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NCI-H28 NWTPO4I3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\TdHJFUUN3ME2yPU4xOTJizszN NYSwb4NFW0GQR1XS
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CAL-54 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzLbm9KSzVyPUK5MlYxPDZizszN MoWyV2FPT0WU
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LS-411N NFHFS2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTNyLkG0O|Uh|ryP NXW3blVKW0GQR1XS
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OVCAR-8 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYr0[2ZDUUN3ME2zNE43Pzl2IN88US=> NIHpTYxUSU6JRWK=
NCI-H630 MoC5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTNyLki5OVMh|ryP MWTTRW5ITVJ?
D-263MG M12xT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnizTWM2OD1|MT6wOFI1KM7:TR?= NE[5bVRUSU6JRWK=
NCI-H596 MkDiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\kdI1KSzVyPUOxMlA3OjFizszN MV7TRW5ITVJ?
BB49-HNC Mn3mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofXTWM2OD1|MT6xNVIh|ryP MV7TRW5ITVJ?
KYSE-70 MomyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LvcWlEPTB;M{GuN|AxQCEQvF2= M1XObXNCVkeHUh?=
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NCI-H1793 NHqw[ZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTN{Lk[xOlMh|ryP MnG0V2FPT0WU
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NCI-H441 NVe5R4hYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTN|LkKyN|Uh|ryP NIfu[|hUSU6JRWK=
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MC-IXC NFy2[XhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzYSopqUUN3ME2zN{42PjV3IN88US=> MlLFV2FPT0WU
NCI-H522 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTN|Lk[1OEDPxE1? NXnxSmNYW0GQR1XS
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NCI-H1417 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{G4T2lEPTB;M{OuPVU5PSEQvF2= MVLTRW5ITVJ?
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HC-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K5cmlEPTB;M{WuOFAzPCEQvF2= NIHn[WJUSU6JRWK=
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BV-173 M4TqTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXwOIJKSzVyPUO1MlgzPjRizszN MVjTRW5ITVJ?
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LNCaP-Clone-FGC MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\jRmlEPTB;M{euNVkxOyEQvF2= NHn1fnRUSU6JRWK=
KARPAS-422 NXm0WHc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DQUWlEPTB;M{euOFQ{OSEQvF2= M3H3VnNCVkeHUh?=
RCM-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLXU2pKSzVyPUO3MlU{PThizszN M3zjRnNCVkeHUh?=
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MDA-MB-453 MofmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\rcJM{UUN3ME2zPU4xOjF6IN88US=> NWHhTng{W0GQR1XS
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SW756 NHjlWopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;Be2lEPTB;M{muOVA6OSEQvF2= Ml65V2FPT0WU
KU-19-19 NHPNWGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXrPZBKSzVyPUO5MlYzQDJizszN NWX1TIdoW0GQR1XS
NCI-H2170 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPmfGNKSzVyPUO5Mlk6PzdizszN MlXTV2FPT0WU
COLO-678 NEe2SYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHKxS2RKSzVyPUSwMlU6PSEQvF2= NGO1PFFUSU6JRWK=
PF-382 NHXEfZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{G1OGlEPTB;NECuO|Q5PyEQvF2= NGjRdIVUSU6JRWK=
OAW-42 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTR{LkK5OFIh|ryP M3;pU3NCVkeHUh?=
LB996-RCC Mn\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vEZWlEPTB;NEKuPFAxQCEQvF2= MV3TRW5ITVJ?
SAS NV3scZJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDJXGZHUUN3ME20N{41Ojh6IN88US=> NX\mO2hWW0GQR1XS
MRK-nu-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILZTYVKSzVyPUS0Mlc1OTRizszN NV7VVopjW0GQR1XS
EoL-1-cell NIjGPZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HDRWlEPTB;NESuPVE{PiEQvF2= M1TRUHNCVkeHUh?=
NCI-H2342 NH;jWmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGL1W4VKSzVyPUS1MlEyOTdizszN NGX3WHhUSU6JRWK=
NCI-H1703 NGn0SnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXmyW5J5UUN3ME20Ok4xPTl{IN88US=> MW\TRW5ITVJ?
NCI-H187 MljLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPFTldoUUN3ME20Ok4yQDZ7IN88US=> MVLTRW5ITVJ?
CP66-MEL MnG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXWTWM2OD12Nj6yO|g6KM7:TR?= MknCV2FPT0WU
HCC1569 NGTRN4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnK1TWM2OD12Nj61PVE4KM7:TR?= NEHydHhUSU6JRWK=
BL-70 M1TQ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXUO3BKSzVyPUS2Mlc4OzdizszN NXHyenpVW0GQR1XS
Saos-2 M3rVUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jDWmlEPTB;NEeuN|EzOyEQvF2= Mm\tV2FPT0WU
LC-1F NFHWXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVL1THVvUUN3ME20O{43OTB3IN88US=> MV3TRW5ITVJ?
KY821 NU\nZmpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHoWoxnUUN3ME20O{43PDN2IN88US=> MYHTRW5ITVJ?
PSN1 M{HKS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TaTWlEPTB;NEiuNVQxOSEQvF2= NGrMbmxUSU6JRWK=
RT4 NH3sSoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4CzUmlEPTB;NEiuPFM4PyEQvF2= M2HqfHNCVkeHUh?=
LS-1034 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{D1cWlEPTB;NEiuPFk2PCEQvF2= M4flPXNCVkeHUh?=
NCI-H1395 NWL0N4hGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTR7LkC0PVIh|ryP MojzV2FPT0WU
EKVX M1PGfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfmUJhSUUN3ME20PU43PzNzIN88US=> M2\Cc3NCVkeHUh?=
IST-MES1 MkTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTR7Lki5OFQh|ryP M1\xWHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

Related FAK Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID