PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
USD 170 In stock
USD 270 In stock
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6 Customer Reviews

  • Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

    Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

    (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

  • D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

    Acta Pharmacol Sin, 2018, doi:10.1038/aps.2017.189. PF-562271 purchased from Selleck.

  • VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

    OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 M3T6d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwMke2OkDPxE1? MoX0V2FPT0WU
SW982 M3qwXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTBwM{K4NkDPxE1? Mk\aV2FPT0WU
KM12 NY\rbXV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWP1Z2N1UUN3ME2wMlM5PTV5IN88US=> MkXEV2FPT0WU
COLO-205 M3H5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHny[GhKSzVyPUCuOFg3PThizszN MVHTRW5ITVJ?
COLO-829 MkTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHsW|dKSzVyPUCuO|UyPzZizszN NXS3SmNbW0GQR1XS
MG-63 NEXkNFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwOEC2N|ch|ryP M3ywVHNCVkeHUh?=
IGROV-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTBwOEGwN|gh|ryP NH:3SnlUSU6JRWK=
NCI-H650 MoT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDWdGdKSzVyPUCuPFMyPTRizszN NGXDUG9USU6JRWK=
RT-112 MnHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTTTWM2OD1yLkm4OFYh|ryP NYj1[2M2W0GQR1XS
BCPAP M2raSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTFwMEGyPFgh|ryP M3vzVnNCVkeHUh?=
ALL-PO MknWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\STWM2OD1zLkCxOVg1KM7:TR?= MUDTRW5ITVJ?
KYSE-270 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PSWWlEPTB;MT6wOFcyPCEQvF2= MmTaV2FPT0WU
8305C MmK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTFwMEm5NFQh|ryP M3\qcnNCVkeHUh?=
NCI-H810 M3G2Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrLeW5QUUN3ME2xMlExPzd4IN88US=> MVvTRW5ITVJ?
CAL-33 NFnGOFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTFwMUK5N|gh|ryP NYjzXYNlW0GQR1XS
AN3-CA NV3lW4NbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvFXXpUUUN3ME2xMlIyQDZ5IN88US=> MVzTRW5ITVJ?
NKM-1 Ml:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTFwMke1NFYh|ryP NVLte2tHW0GQR1XS
BPH-1 MlXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjsXJRKSzVyPUGuNlg4PjZizszN MVPTRW5ITVJ?
MES-SA NELtW|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTFwM{C2PFIh|ryP M1HafnNCVkeHUh?=
CAL-62 M1rwWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfOTWM2OD1zLkOxPVA6KM7:TR?= NHvjTFNUSU6JRWK=
Detroit562 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4[zcmlEPTB;MT6zOVIzOiEQvF2= M3XNNXNCVkeHUh?=
KYSE-150 NIXVdZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH30T4pKSzVyPUGuN|UzOzZizszN NXPkV4t5W0GQR1XS
SK-UT-1 NV3kd4ttT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nRdWlEPTB;MT60OFY1PyEQvF2= M2\SenNCVkeHUh?=
HUTU-80 M2rF[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXPTWM2OD1zLkS0PFg3KM7:TR?= NUGwfYMzW0GQR1XS
SIG-M5 M37IPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIKwNm5KSzVyPUGuOFg{QDdizszN M3PLZnNCVkeHUh?=
AGS M{LpbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTFwNUKxNlQh|ryP M3naVXNCVkeHUh?=
ST486 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPacWlKSzVyPUGuOVMzPzhizszN MkHiV2FPT0WU
HSC-2 NInHUWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4r6RWlEPTB;MT61N|k2KM7:TR?= M{XaXnNCVkeHUh?=
LCLC-103H NVT3O4V[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[3fnpKSzVyPUGuOVU5OThizszN MVXTRW5ITVJ?
BC-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPJVXFKSzVyPUGuOlE3PjRizszN M1\vOnNCVkeHUh?=
CGTH-W-1 M2TFN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHoTWM2OD1zLk[xOlc6KM7:TR?= NF;U[pJUSU6JRWK=
MZ1-PC M2G2fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PFe2lEPTB;MT62NlMyOiEQvF2= MoXCV2FPT0WU
SW1710 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2f0RmlEPTB;MT62NlYzQCEQvF2= MkjzV2FPT0WU
EW-13 M4HwfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnPcIZ7UUN3ME2xMlY{PDZ4IN88US=> MlTCV2FPT0WU
TE-11 Mmj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTFwNkO1NlIh|ryP NHnQbVJUSU6JRWK=
BHY MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ntc2lEPTB;MT62OVY3OyEQvF2= NY\LOGtzW0GQR1XS
ML-2 NVLYVmJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTFwNk[4NFIh|ryP M3HnSnNCVkeHUh?=
MEL-JUSO NXrMemgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXqOY9pUUN3ME2xMlczQDlzIN88US=> NHTFWIFUSU6JRWK=
U251 M2HYbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jvOGlEPTB;MT63OFA{OSEQvF2= MmPxV2FPT0WU
MHH-ES-1 M2rLfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTFwN{m1Olgh|ryP NHv1WVVUSU6JRWK=
CAL-85-1 NUf0VGZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTFwOEC0PUDPxE1? MUnTRW5ITVJ?
D-336MG NFr6WHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF20Z2FKSzVyPUGuPFU3OTRizszN MXPTRW5ITVJ?
VA-ES-BJ M1PtNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PZSmlEPTB;MT65NFMyPSEQvF2= NGq0S29USU6JRWK=
MDA-MB-468 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moq1TWM2OD1zLkmxNlczKM7:TR?= MXXTRW5ITVJ?
MEL-HO MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHaSZdKSzVyPUGuPVc6QTNizszN M3W4PHNCVkeHUh?=
SW954 M2\SfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJwMECxOVEh|ryP MkftV2FPT0WU
LB831-BLC MoX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfnTWM2OD1{LkCwPVg5KM7:TR?= MlHJV2FPT0WU
EW-16 NY\sVlU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLkNmxKSzVyPUKuNFM6QDNizszN M332WXNCVkeHUh?=
NCI-H460 NFfxdFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrkTWM2OD1{LkC0PFM6KM7:TR?= NH3yNWRUSU6JRWK=
CHP-212 NGHR[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;NTWM2OD1{LkC5OVM6KM7:TR?= M{mwSHNCVkeHUh?=
MHH-NB-11 M2jwbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF63OpdKSzVyPUKuNVI5OThizszN NF20WnJUSU6JRWK=
DU-4475 NYDj[m1yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fIXWlEPTB;Mj6xOFc2QSEQvF2= NUHESIJ5W0GQR1XS
ABC-1 NIS0PGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTJwMkWxPVYh|ryP NXvFUXM5W0GQR1XS
LOUCY M2PKdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTJwMk[xNlgh|ryP MljRV2FPT0WU
LC-2-ad M4PEfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mli4TWM2OD1{LkK2PVY3KM7:TR?= M2L1SHNCVkeHUh?=
ETK-1 NVP3SI5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTWTWM2OD1{LkK4OFYh|ryP MWrTRW5ITVJ?
KYSE-180 Mm\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTJwMki4O|Qh|ryP M2r3NHNCVkeHUh?=
HOP-92 MonHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LXTWlEPTB;Mj6zNFY4OyEQvF2= NV\XZ4s2W0GQR1XS
RCC10RGB NYTidohrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnIcnpKSzVyPUKuN|I2ODdizszN MkfBV2FPT0WU
KASUMI-1 MlPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXqVVdYUUN3ME2yMlMzQDhizszN MnfSV2FPT0WU
ACN NHixWG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjJR|UxRTJwM{S3OFUh|ryP M4nIUXNCVkeHUh?=
BxPC-3 NWHSfoFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PlN2lEPTB;Mj6zOVQ{PiEQvF2= NWq4[5ZJW0GQR1XS
MONO-MAC-6 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTJwNEGyOFgh|ryP MmThV2FPT0WU
CAL-39 NFnOZ4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXrPZVKSzVyPUKuOFE3OjhizszN NVPiepJEW0GQR1XS
CMK Mnj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPxe4toUUN3ME2yMlQyPzl3IN88US=> MoXkV2FPT0WU
MFE-296 M1rFbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTJwNEe3PVIh|ryP NGPIXWZUSU6JRWK=
DU-145 M2m1dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHlVoJUUUN3ME2yMlQ6OTF6IN88US=> MlvWV2FPT0WU
MDA-MB-231 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjpfmVKSzVyPUKuOFk2PzJizszN Mm[yV2FPT0WU
EFO-27 NU\XeZBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3q5SGlEPTB;Mj61NVg5QCEQvF2= NUnQd2NWW0GQR1XS
SNU-387 Mn;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTJwNUK4NkDPxE1? NES1XGpUSU6JRWK=
KYSE-410 NIHM[m5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXUV2ZZUUN3ME2yMlU3PTF5IN88US=> NGWxSmxUSU6JRWK=
NCCIT M2jRSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIS3WHJKSzVyPUKuOVczPCEQvF2= MX;TRW5ITVJ?
LXF-289 M4L0R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;MWY81UUN3ME2yMlU5Ojd|IN88US=> NF;CXFBUSU6JRWK=
HMV-II MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYP2OWNSUUN3ME2yMlU5QTl5IN88US=> MkXHV2FPT0WU
MOLT-4 M1vtZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTJwNkC3NVIh|ryP MmjyV2FPT0WU
KOSC-2 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;SXZVtUUN3ME2yMlY{QDd7IN88US=> NH7nNJdUSU6JRWK=
TUR NU\DVIxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTJwNkWzOlkh|ryP MXjTRW5ITVJ?
NCI-H2087 Mk\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nsb2lEPTB;Mj63NVY1PiEQvF2= M17O[3NCVkeHUh?=
SCC-15 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPFdnlrUUN3ME2yMlc2OjV{IN88US=> MnPUV2FPT0WU
HSC-4 M1fWVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTJwN{izPFkh|ryP M3HRNXNCVkeHUh?=
G-402 M3zuc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjNdGh1UUN3ME2yMlgyPDR5IN88US=> M{m4fHNCVkeHUh?=
GP5d Mk\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGizOoNKSzVyPUKuPFM5OjdizszN MYDTRW5ITVJ?
IGR-1 MmHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3sbZYxUUN3ME2yMlg{QTh5IN88US=> M4XSVnNCVkeHUh?=
SK-PN-DW NE\SPZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTJwOE[yNFQh|ryP M3[zXnNCVkeHUh?=
OPM-2 NVToWZlGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTwcVNVUUN3ME2yMlg6QTd6IN88US=> NH31N41USU6JRWK=
5637 M3j5Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHlSYlKSzVyPUKuPVI1OzlizszN MUjTRW5ITVJ?
NCI-H2030 Mn73S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnr3TWM2OD1{Lkm4NFI6KM7:TR?= M1zpWHNCVkeHUh?=
SK-LU-1 NISyNWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjQb4ZIUUN3ME2yMlk5OzN2IN88US=> NIWxPYhUSU6JRWK=
EW-18 NHzw[|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXofGFKSzVyPUOuNFU{OiEQvF2= MUfTRW5ITVJ?
786-0 NFjVU5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfiNHFKSzVyPUOuNFU4QDRizszN Ml3ZV2FPT0WU
NCI-H1963 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\JfW5KSzVyPUOuNFg5OjVizszN MXvTRW5ITVJ?
A2780 MoP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml[wTWM2OD1|LkGwN|QyKM7:TR?= MXnTRW5ITVJ?
NCI-H526 MmrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPGWXVKSzVyPUOuNVM6PjFizszN MXLTRW5ITVJ?
BHT-101 M3\yVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTNwMUW3PVgh|ryP NEi1cVZUSU6JRWK=
RPMI-7951 NGrsT3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7qTWM2OD1|LkKwNVM4KM7:TR?= NUCxOZllW0GQR1XS
HL-60 M3i0OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4SzZmlEPTB;Mz6yNVE1KM7:TR?= NH;SWWpUSU6JRWK=
CTV-1 M2j1cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHHNJBKSzVyPUOuNlU{PzdizszN NVm0VGVvW0GQR1XS
IST-SL1 NV;qcGtST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDuXG9ZUUN3ME2zMlI6PDZ3IN88US=> M4rDZXNCVkeHUh?=
G-401 NUjtd3c2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDzRoVrUUN3ME2zMlI6QDB6IN88US=> NHvzfGNUSU6JRWK=
OVCAR-5 NUnBR2FuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTNwM{[zOVIh|ryP NH7VPGdUSU6JRWK=
EW-1 M1T5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfqO3lKSzVyPUOuOFA2PDRizszN MV;TRW5ITVJ?
H4 Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTNwNEe1Olkh|ryP MXLTRW5ITVJ?
EM-2 MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnJNZZKSzVyPUOuOFg3PzRizszN Mm[zV2FPT0WU
RS4-11 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYOxSI5LUUN3ME2zMlQ5PzF3IN88US=> NEPCT2ZUSU6JRWK=
NOMO-1 MnHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXqR4VKSzVyPUOuOFkzODRizszN NYDXSZpMW0GQR1XS
HCT-116 NUDEU4dMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LSUGlEPTB;Mz61NFcyOSEQvF2= MUXTRW5ITVJ?
CAL-120 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfTW|lMUUN3ME2zMlU4OzVizszN NXPzVoFyW0GQR1XS
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NCI-H524 MlryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzERlRLUUN3ME2xPU42PzJ3IN88US=> NHftVFFUSU6JRWK=
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NCI-H2122 NHTIOGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTJ{LkG2O|ch|ryP M3nz[nNCVkeHUh?=
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NCI-H727 NH23eGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfYTWM2OD1{Mj64PFM3KM7:TR?= Mk\hV2FPT0WU
SW1573 M1rQNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUi3THJLUUN3ME2yN{4xPjR4IN88US=> MnLEV2FPT0WU
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MC-CAR M4DXVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTJ|LkWxPFMh|ryP NHniNHVUSU6JRWK=
NCI-H446 M3nk[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fH[GlEPTB;MkOuOlA6OyEQvF2= M1LBfnNCVkeHUh?=
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SW684 M1rrUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDmSHNsUUN3ME2yOE44PjJ2IN88US=> MVrTRW5ITVJ?
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CCRF-CEM MoHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHwbJhDUUN3ME2yOU44PDV7IN88US=> NGTjSVJUSU6JRWK=
CAPAN-1 NULrc|VtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTJ3Lke1NFEh|ryP M{\WRXNCVkeHUh?=
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LU-99A M2W0S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTJSox5UUN3ME2yO{4yPzV2IN88US=> NFPUO|FUSU6JRWK=
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HDLM-2 NH:3[IJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTJ6LkK0OVkh|ryP MnzYV2FPT0WU
NCI-H1651 NHXNToJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTJ6LkK1PVIh|ryP NHTjc3dUSU6JRWK=
SK-N-AS M4LucWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7DbI9KSzVyPUK4MlMyPzFizszN MWjTRW5ITVJ?
NY MonWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2SyUWlEPTB;MkiuOlU4QSEQvF2= M3fXWnNCVkeHUh?=
D-283MED Mo\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\ITWM2OD1{OD64O|A5KM7:TR?= MVXTRW5ITVJ?
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NCI-H28 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPDTWM2OD1{OT6wNVIh|ryP NH\KW|dUSU6JRWK=
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SK-MEL-24 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnf6TWM2OD1{OT61OFY3KM7:TR?= NVTGNIMxW0GQR1XS
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OVCAR-8 NVrMNXVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUW2PZZWUUN3ME2zNE43Pzl2IN88US=> NXTsfGVpW0GQR1XS
NCI-H630 M4jOS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknvTWM2OD1|MD64PVU{KM7:TR?= MWXTRW5ITVJ?
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NCI-H596 MorQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTuTWM2OD1|MT6wOlIyKM7:TR?= MmDoV2FPT0WU
BB49-HNC NF;CXJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPoTWM2OD1|MT6xNVIh|ryP MojPV2FPT0WU
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LAN-6 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{naZWlEPTB;M{GuOFk6OiEQvF2= M1KzeHNCVkeHUh?=
NCI-H2228 NYXzUnR4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\ITWM2OD1|MT61OlI2KM7:TR?= NGTKR5FUSU6JRWK=
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DJM-1 M2\2c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTN{LkGzPFYh|ryP MXXTRW5ITVJ?
SW48 MnLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDPb4k1UUN3ME2zNk4{QDJ3IN88US=> NGfic21USU6JRWK=
NCI-H1793 NWi3WmFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTN{Lk[xOlMh|ryP MXTTRW5ITVJ?
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NCI-H441 M4XSemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fTXWlEPTB;M{OuNlI{PSEQvF2= NGrWZW5USU6JRWK=
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MC-IXC MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTN|LkW2OVUh|ryP MXfTRW5ITVJ?
NCI-H522 NFjYUmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TqNWlEPTB;M{OuOlU1KM7:TR?= MYfTRW5ITVJ?
IA-LM MlX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;6T5B7UUN3ME2zN{43PzR4IN88US=> MVzTRW5ITVJ?
NCI-H1417 M{L2Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\XWYlKSzVyPUOzMlk2QDVizszN MUPTRW5ITVJ?
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HC-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnwTWM2OD1|NT60NFI1KM7:TR?= NWiwZZp4W0GQR1XS
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EFE-184 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{T3ZmlEPTB;M{WuOlgzQSEQvF2= MVjTRW5ITVJ?
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SW1463 M1fRbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\yXGlEPTB;M{[uOlU3PiEQvF2= MXjTRW5ITVJ?
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HCC1937 Mn\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHoZWRmUUN3ME2zO{4xOTV2IN88US=> NYG4d5NiW0GQR1XS
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RCM-1 M2j3bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3JTWM2OD1|Nz61N|U5KM7:TR?= Mki3V2FPT0WU
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KU-19-19 NV[4[WVNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnO5TWM2OD1|OT62NlgzKM7:TR?= MUnTRW5ITVJ?
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COLO-678 NVnqVW5ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\5SXZxUUN3ME20NE42QTVizszN NIX2eG9USU6JRWK=
PF-382 NUHr[JJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmL5TWM2OD12MD63OFg4KM7:TR?= MXrTRW5ITVJ?
OAW-42 M2TrUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYixWW92UUN3ME20Nk4zQTR{IN88US=> NYPMN5p5W0GQR1XS
LB996-RCC NXO3XVR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1r5PWlEPTB;NEKuPFAxQCEQvF2= M4nRXXNCVkeHUh?=
SAS NWTM[4p5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7BZlV[UUN3ME20N{41Ojh6IN88US=> NV3md|NoW0GQR1XS
MRK-nu-1 NIXodXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTR2Lke0NVQh|ryP NHPMNm5USU6JRWK=
EoL-1-cell MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPzTWM2OD12ND65NVM3KM7:TR?= NWj0cWxsW0GQR1XS
NCI-H2342 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4e2bWlEPTB;NEWuNVEyPyEQvF2= MkfIV2FPT0WU
NCI-H1703 NGD0OHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17kTWlEPTB;NE[uNFU6OiEQvF2= MlH1V2FPT0WU
NCI-H187 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\HS3BjUUN3ME20Ok4yQDZ7IN88US=> M2\wR3NCVkeHUh?=
CP66-MEL MmLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTR4LkK3PFkh|ryP NYLRU5ppW0GQR1XS
HCC1569 Ml76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHsUmZKSzVyPUS2MlU6OTdizszN M{fDU3NCVkeHUh?=
BL-70 M2LHOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHkcnVrUUN3ME20Ok44PzN5IN88US=> M2H0[XNCVkeHUh?=
Saos-2 NF20O45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITFcpZKSzVyPUS3MlMyOjNizszN NU\adHdnW0GQR1XS
LC-1F NHfGcINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrUTWM2OD12Nz62NVA2KM7:TR?= MUfTRW5ITVJ?
KY821 NYS4bG1[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrIdHNKSzVyPUS3MlY1OzRizszN MYfTRW5ITVJ?
PSN1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjkO4VKSzVyPUS4MlE1ODFizszN Mnn4V2FPT0WU
RT4 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfmOWU1UUN3ME20PE45Ozd5IN88US=> NYC5TVEyW0GQR1XS
LS-1034 M3LzT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrMT2V[UUN3ME20PE45QTV2IN88US=> M1fic3NCVkeHUh?=
NCI-H1395 NHyzZoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrhSHdKSzVyPUS5MlA1QTJizszN NGrsUGRUSU6JRWK=
EKVX MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7WPFFKSzVyPUS5MlY4OzFizszN Mn[5V2FPT0WU
IST-MES1 M{HCU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTR7Lki5OFQh|ryP MUTTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

Related FAK Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID