PF-562271

Catalog No.S2890

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

6 Customer Reviews

Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

(F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.
D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

Acta Pharmacol Sin, 2018, doi:10.1038/aps.2017.189. PF-562271 purchased from Selleck.
VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description

Targets

FAK ^[1] (Cell-free assay)	PYK2 ^[1] (Cell-free assay)	CDK2/CyclinE ^[1] (Cell-free assay)	CDK3/CyclinE ^[1] (Cell-free assay)	CDK1/CyclinB ^[1] (Cell-free assay)	View More
1.5 nM	13 nM	30 nM	47 nM	58 nM	View More

In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. ^[1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. ^[2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. ^[3]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
MV-4-11	Ml2yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1rqXmlEPTB;MD6yO\|Y3KM7:TR?=	NFr3PHpUSU6JRWK=
SW982	NIPmUVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn\kTWM2OD1yLkOyPFIh\|ryP	M2LEXnNCVkeHUh?=
KM12	Mn3pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYXJR\|UxRTBwM{i1OVch\|ryP	NFu5XJJUSU6JRWK=
COLO-205	NEHofopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1PONWlEPTB;MD60PFY2QCEQvF2=	M3XmPHNCVkeHUh?=
COLO-829	MljTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NH73THZKSzVyPUCuO\|UyPzZizszN	M17XWXNCVkeHUh?=
MG-63	M3f4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MUDJR\|UxRTBwOEC2N\|ch\|ryP	NES4bpBUSU6JRWK=
IGROV-1	NVrH[\|NuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHTOXVRKSzVyPUCuPFExOzhizszN	NYLCXZVzW0GQR1XS
NCI-H650	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3Ww[GlEPTB;MD64N\|E2PCEQvF2=	NYjYS5ROW0GQR1XS
RT-112	NX7mSVRyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MULJR\|UxRTBwOUi0OkDPxE1?	MmrIV2FPT0WU
BCPAP	M4\XUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3zvemlEPTB;MT6wNVI5QCEQvF2=	NVP0Om84W0GQR1XS
ALL-PO	M1Hqcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NIfz[HZKSzVyPUGuNFE2QDRizszN	NYXsSlV5W0GQR1XS
KYSE-270	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MUjJR\|UxRTFwMES3NVQh\|ryP	MkO3V2FPT0WU
8305C	NVXUUVA{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoWzTWM2OD1zLkC5PVA1KM7:TR?=	M4HvOXNCVkeHUh?=
NCI-H810	MlnIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NITmZVdKSzVyPUGuNVA4PzZizszN	MWLTRW5ITVJ?
CAL-33	M2DOS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYrCO5BiUUN3ME2xMlEzQTN6IN88US=>	NWXKPYZRW0GQR1XS
AN3-CA	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYrJR\|UxRTFwMkG4Olch\|ryP	NEXsSFNUSU6JRWK=
NKM-1	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFzZTHJKSzVyPUGuNlc2ODZizszN	NV;CeGQ4W0GQR1XS
BPH-1	MnvFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVn1NJRkUUN3ME2xMlI5PzZ4IN88US=>	MmXKV2FPT0WU
MES-SA	Mnm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVH3XnA6UUN3ME2xMlMxPjh{IN88US=>	MUHTRW5ITVJ?
CAL-62	NGXr[JBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NEHGOJJKSzVyPUGuN\|E6ODlizszN	NF\kfGNUSU6JRWK=
Detroit562	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1v2dmlEPTB;MT6zOVIzOiEQvF2=	MkHuV2FPT0WU
KYSE-150	M3TzVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWrJR\|UxRTFwM{WyN\|Yh\|ryP	NYjmW411W0GQR1XS
SK-UT-1	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmrVTWM2OD1zLkS0OlQ4KM7:TR?=	NGGzN2dUSU6JRWK=
HUTU-80	MnezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MUPJR\|UxRTFwNES4PFYh\|ryP	NVi0N2JbW0GQR1XS
SIG-M5	Mn7LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYXJR\|UxRTFwNEizPFch\|ryP	M3XLT3NCVkeHUh?=
AGS	NIWwco9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUT6PGlRUUN3ME2xMlUzOTJ2IN88US=>	NWXaSXU4W0GQR1XS
ST486	MoHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXjQUopLUUN3ME2xMlU{Ojd6IN88US=>	MUPTRW5ITVJ?
HSC-2	NEHBTlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MV3JR\|UxRTFwNUO5OUDPxE1?	M3;JdXNCVkeHUh?=
LCLC-103H	MkX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MmTTTWM2OD1zLkW1PFE5KM7:TR?=	NYH0ZlkxW0GQR1XS
BC-1	M17ZdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHrZR\|BKSzVyPUGuOlE3PjRizszN	NIf4Zo5USU6JRWK=
CGTH-W-1	NXzzbXhKT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MYXJR\|UxRTFwNkG2O\|kh\|ryP	MnvBV2FPT0WU
MZ1-PC	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWnJR\|UxRTFwNkKzNVIh\|ryP	M17L[HNCVkeHUh?=
SW1710	MkDRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFTuWVZKSzVyPUGuOlI3OjhizszN	NF6zXGtUSU6JRWK=
EW-13	MmO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFjNV4dKSzVyPUGuOlM1PjZizszN	MWfTRW5ITVJ?
TE-11	M3S4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEfxN4RKSzVyPUGuOlM2OjJizszN	MnfVV2FPT0WU
BHY	MnGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mn\RTWM2OD1zLk[1OlY{KM7:TR?=	M1fTb3NCVkeHUh?=
ML-2	MlT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{PMeWlEPTB;MT62OlgxOiEQvF2=	MmflV2FPT0WU
MEL-JUSO	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3;xeGlEPTB;MT63Nlg6OSEQvF2=	MUTTRW5ITVJ?
U251	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmXLTWM2OD1zLke0NFMyKM7:TR?=	MoWwV2FPT0WU
MHH-ES-1	MlH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Ml;qTWM2OD1zLke5OVY5KM7:TR?=	NYrGNmVbW0GQR1XS
CAL-85-1	MoPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVzJR\|UxRTFwOEC0PUDPxE1?	NXe0VGxzW0GQR1XS
D-336MG	NWG3cWVXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Ml3sTWM2OD1zLki1OlE1KM7:TR?=	MknDV2FPT0WU
VA-ES-BJ	NYX6VFlvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MU\JR\|UxRTFwOUCzNVUh\|ryP	NFKxNIRUSU6JRWK=
MDA-MB-468	MmjRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVvJR\|UxRTFwOUGyO\|Ih\|ryP	MnPIV2FPT0WU
MEL-HO	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MkHZTWM2OD1zLkm3PVk{KM7:TR?=	MVXTRW5ITVJ?
SW954	NXnkZXZTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NEf3RVZKSzVyPUKuNFAyPTFizszN	NGXLbIlUSU6JRWK=
LB831-BLC	NETOOmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NULpeIdGUUN3ME2yMlAxQTh6IN88US=>	MVvTRW5ITVJ?
EW-16	NUG0cm03T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoHETWM2OD1{LkCzPVg{KM7:TR?=	MmXiV2FPT0WU
NCI-H460	M4Xwc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NWr3foZzUUN3ME2yMlA1QDN7IN88US=>	Ml;HV2FPT0WU
CHP-212	MlT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXvJR\|UxRTJwMEm1N\|kh\|ryP	NHLF[21USU6JRWK=
MHH-NB-11	MoS3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NIXLeVRKSzVyPUKuNVI5OThizszN	NUD2OYVCW0GQR1XS
DU-4475	M3XITmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MUXJR\|UxRTJwMUS3OVkh\|ryP	NYjOUos2W0GQR1XS
ABC-1	NWraU5RuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NH7YW\|NKSzVyPUKuNlUyQTZizszN	NF3FfolUSU6JRWK=
LOUCY	NHjMVmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkTPTWM2OD1{LkK2NVI5KM7:TR?=	MoT1V2FPT0WU
LC-2-ad	NHfpdIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFrIWFZKSzVyPUKuNlY6PjZizszN	MnfDV2FPT0WU
ETK-1	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoDxTWM2OD1{LkK4OFYh\|ryP	NHn2O\|lUSU6JRWK=
KYSE-180	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYrqfFJwUUN3ME2yMlI5QDd2IN88US=>	NF:zUpVUSU6JRWK=
HOP-92	M2DPW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mn;6TWM2OD1{LkOwOlc{KM7:TR?=	MmTHV2FPT0WU
RCC10RGB	MnjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MofqTWM2OD1{LkOyOVA4KM7:TR?=	NUnjc21KW0GQR1XS
KASUMI-1	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXnJR\|UxRTJwM{K4PEDPxE1?	MkP0V2FPT0WU
ACN	M3XJTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MUXJR\|UxRTJwM{S3OFUh\|ryP	M37J[3NCVkeHUh?=
BxPC-3	NWLib2dXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1;nNGlEPTB;Mj6zOVQ{PiEQvF2=	MnLtV2FPT0WU
MONO-MAC-6	NXH2bVNxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkfOTWM2OD1{LkSxNlQ5KM7:TR?=	MlvhV2FPT0WU
CAL-39	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXHJR\|UxRTJwNEG2Nlgh\|ryP	MlK5V2FPT0WU
CMK	MnPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MW\JR\|UxRTJwNEG3PVUh\|ryP	NH60fm9USU6JRWK=
MFE-296	NYLyb5FNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mo\ZTWM2OD1{LkS3O\|kzKM7:TR?=	NUfqNmtHW0GQR1XS
DU-145	Mn2wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NW\5SHhrUUN3ME2yMlQ6OTF6IN88US=>	M3G2ZXNCVkeHUh?=
MDA-MB-231	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXjMfJlvUUN3ME2yMlQ6PTd{IN88US=>	MUHTRW5ITVJ?
EFO-27	Mof2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnznTWM2OD1{LkWxPFg5KM7:TR?=	MUHTRW5ITVJ?
SNU-387	MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUXHVJpJUUN3ME2yMlUzQDJizszN	NUjP[IluW0GQR1XS
KYSE-410	M4jsbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVrU[\|N2UUN3ME2yMlU3PTF5IN88US=>	M2XzeXNCVkeHUh?=
NCCIT	NFj5VXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUHJR\|UxRTJwNUeyOEDPxE1?	NFjjUWtUSU6JRWK=
LXF-289	MnGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MUjJR\|UxRTJwNUiyO\|Mh\|ryP	Mk\oV2FPT0WU
HMV-II	MmPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1yyOWlEPTB;Mj61PFk6PyEQvF2=	M2XrXnNCVkeHUh?=
MOLT-4	M4XUS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVGwPVFbUUN3ME2yMlYxPzF{IN88US=>	MUHTRW5ITVJ?
KOSC-2	MmTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVLJR\|UxRTJwNkO4O\|kh\|ryP	MkXEV2FPT0WU
TUR	NHfINGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUXLPIppUUN3ME2yMlY2OzZ7IN88US=>	MkPQV2FPT0WU
NCI-H2087	MkjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MX3JR\|UxRTJwN{G2OFYh\|ryP	M2TLeXNCVkeHUh?=
SCC-15	NGTJUYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MV;JR\|UxRTJwN{WyOVIh\|ryP	MoHUV2FPT0WU
HSC-4	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4LBXGlEPTB;Mj63PFM5QSEQvF2=	MUPTRW5ITVJ?
G-402	NUPrbXM5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MlzVTWM2OD1{LkixOFQ4KM7:TR?=	MYfTRW5ITVJ?
GP5d	NYLnPZpXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWrJR\|UxRTJwOEO4Nlch\|ryP	MUXTRW5ITVJ?
IGR-1	NFzlN3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVvJR\|UxRTJwOEO5PFch\|ryP	M17neXNCVkeHUh?=
SK-PN-DW	NXj1NYo3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVTqUpluUUN3ME2yMlg3OjB2IN88US=>	MX7TRW5ITVJ?
OPM-2	MnfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXmxVWZbUUN3ME2yMlg6QTd6IN88US=>	Mo\KV2FPT0WU
5637	M1i4V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1Tt[2lEPTB;Mj65NlQ{QSEQvF2=	NFXQNZhUSU6JRWK=
NCI-H2030	Mn2zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXPJR\|UxRTJwOUiwNlkh\|ryP	NGDZUIVUSU6JRWK=
SK-LU-1	MmPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUT0VGlDUUN3ME2yMlk5OzN2IN88US=>	MVnTRW5ITVJ?
EW-18	NYX0S3R4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MULJR\|UxRTNwMEWzNkDPxE1?	MYHTRW5ITVJ?
786-0	NWrHXZBjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3TYRmlEPTB;Mz6wOVc5PCEQvF2=	MV\TRW5ITVJ?
NCI-H1963	M4q3VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFTUN2hKSzVyPUOuNFg5OjVizszN	NXLrenFMW0GQR1XS
A2780	MlG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFH1WlBKSzVyPUOuNVA{PDFizszN	M2PKNnNCVkeHUh?=
NCI-H526	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1TjZmlEPTB;Mz6xN\|k3OSEQvF2=	MVHTRW5ITVJ?
BHT-101	NH;PWG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MWrJR\|UxRTNwMUW3PVgh\|ryP	NET0NZBUSU6JRWK=
RPMI-7951	M4LUUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3radGlEPTB;Mz6yNFE{PyEQvF2=	M17pPXNCVkeHUh?=
HL-60	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmnRTWM2OD1\|LkKxNVQh\|ryP	MnjwV2FPT0WU
CTV-1	Mk\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MX3JR\|UxRTNwMkWzO\|ch\|ryP	MVfTRW5ITVJ?
IST-SL1	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGHU[XJKSzVyPUOuNlk1PjVizszN	NWXDV\|NIW0GQR1XS
G-401	MoXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVLJR\|UxRTNwMkm4NFgh\|ryP	MmDEV2FPT0WU
OVCAR-5	MkK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{njOmlEPTB;Mz6zOlM2OiEQvF2=	NIP2SFRUSU6JRWK=
EW-1	NIX1OHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHP1UmZKSzVyPUOuOFA2PDRizszN	NG\ITVFUSU6JRWK=
H4	NUHLe3pJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVrlTZduUUN3ME2zMlQ4PTZ7IN88US=>	Mn7GV2FPT0WU
EM-2	NX;vOnllT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1;M[WlEPTB;Mz60PFY4PCEQvF2=	M{jaUnNCVkeHUh?=
RS4-11	MojpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlqzTWM2OD1\|LkS4O\|E2KM7:TR?=	MXfTRW5ITVJ?
NOMO-1	MkG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYPJR\|UxRTNwNEmyNFQh\|ryP	NF\qSZlUSU6JRWK=
HCT-116	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYHJR\|UxRTNwNUC3NVEh\|ryP	NXPLZWlIW0GQR1XS
CAL-120	M1jQTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGfYenNKSzVyPUOuOVc{PSEQvF2=	M{HCVHNCVkeHUh?=
CAL-51	NHLsUIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3LDRWlEPTB;Mz61O\|YyOiEQvF2=	NF23d\|BUSU6JRWK=
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NCI-H2081	NXfRN2IxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MY\JR\|UxRTNwNkWyPFgh\|ryP	MXTTRW5ITVJ?
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NCI-H747	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnPuTWM2OD12LkC4Olc1KM7:TR?=	MnG0V2FPT0WU
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COR-L23	MmPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXrJR\|UxRTRwNkeyPEDPxE1?	M3\ZdXNCVkeHUh?=
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NCI-H2009	MnjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYDo[2FQUUN3ME21MlMxPTN6IN88US=>	M4LCXXNCVkeHUh?=
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IST-SL2	M3zpTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NWrCNYZJUUN3ME23MlUzOjd{IN88US=>	MlPIV2FPT0WU
MLMA	NFTNcpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mny4TWM2OD15LkW0Olg6KM7:TR?=	NVjkTpdUW0GQR1XS
22RV1	NWrDUZBVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVfUfmRZUUN3ME23MlY4QTd3IN88US=>	NHXGXVBUSU6JRWK=
ONS-76	Mn62S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2j3[2lEPTB;Nz62PVQyPiEQvF2=	NHH0ellUSU6JRWK=
ECC12	NFToTpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3fyVmlEPTB;Nz63NlkxOSEQvF2=	NWj0TIN6W0GQR1XS
HT-1080	NGfqXXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmTNTWM2OD15Lke4OVU2KM7:TR?=	MYjTRW5ITVJ?
SF268	Moe0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3\OemlEPTB;Nz64N\|U3KM7:TR?=	MVfTRW5ITVJ?
COLO-800	NXzXN3c5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmrHTWM2OD15LkmwOlg2KM7:TR?=	NHrqTnhUSU6JRWK=
BB30-HNC	MoLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYP4VWdtUUN3ME23Mlk1PTV\|IN88US=>	Mn\rV2FPT0WU
CAKI-1	M1q3[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{G5eWlEPTB;OD6wOlUzOSEQvF2=	MmDrV2FPT0WU
GCIY	NVn1WllRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGLDU2JKSzVyPUiuNlM4OTNizszN	MV3TRW5ITVJ?
MKN28	M1fUd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEPqVYZKSzVyPUiuNlQ2QThizszN	M1fzdnNCVkeHUh?=
IPC-298	NFHFflZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4OxTGlEPTB;OD6yO\|cxQSEQvF2=	NIjmRYxUSU6JRWK=
NCI-H1650	NUXKW5pWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoOzTWM2OD16LkK5NVg2KM7:TR?=	NWL4RZVWW0GQR1XS
KP-N-YS	M{TDd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYrJR\|UxRThwMkmzNFgh\|ryP	NVvxbXB2W0GQR1XS
J82	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MkfPTWM2OD16LkO0NVI1KM7:TR?=	MX;TRW5ITVJ?
SK-HEP-1	NX;JTldQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MULJR\|UxRThwM{W3NlMh\|ryP	M1rRbHNCVkeHUh?=
PANC-03-27	NF62SW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXvJR\|UxRThwM{[5NkDPxE1?	MVzTRW5ITVJ?
KP-4	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnL2TWM2OD16LkSxPFU{KM7:TR?=	MoK2V2FPT0WU
RERF-LC-MS	M1[zPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXLJR\|UxRThwNEi1PVUh\|ryP	MoO2V2FPT0WU
C-33-A	M{j6cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGHCZpZKSzVyPUiuOlE2OjlizszN	M37aW3NCVkeHUh?=
PA-1	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NF21RmFKSzVyPUiuOlY3QTRizszN	MXTTRW5ITVJ?
TE-5	M3PtcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXjJR\|UxRThwN{CyPVYh\|ryP	M2nVbnNCVkeHUh?=
MHH-PREB-1	MkjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MofOTWM2OD16LkezNVA4KM7:TR?=	MoTtV2FPT0WU
C3A	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEPsUZNKSzVyPUiuO\|Y2PjFizszN	MWfTRW5ITVJ?
SK-OV-3	MknOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGHCcY5KSzVyPUiuO\|k4PzJizszN	MVfTRW5ITVJ?
AM-38	M2qxTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYHJR\|UxRThwOEC0N\|kh\|ryP	NVzFSnZ{W0GQR1XS
LoVo	MnfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MV3JR\|UxRThwOUW1OlYh\|ryP	M3W2eXNCVkeHUh?=
MKN45	NXHpUHNOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3;SbmlEPTB;OD65OlA3OyEQvF2=	NWHLdJo3W0GQR1XS
NH-12	Mlf2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NIHCUJdKSzVyPUmuNFI2PDJizszN	NWXDRmdsW0GQR1XS
CAMA-1	NGTueJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYLFVo16UUN3ME25MlA3ODF{IN88US=>	MlzJV2FPT0WU
HT-29	NIjpUYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWGwPYtYUUN3ME25MlEzOzd\|IN88US=>	NVnr[Y42W0GQR1XS
DMS-273	NYDxNVJMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NV3NWJJWUUN3ME25MlEzPSEQvF2=	NWfST5dwW0GQR1XS
NCI-H719	NIG5SYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mk\yTWM2OD17LkG4N\|kyKM7:TR?=	NUfCTZpRW0GQR1XS
SF539	M{PZTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MkfMTWM2OD17LkKxNFk5KM7:TR?=	NFrQUHZUSU6JRWK=
Daoy	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mom5TWM2OD17LkK0NVQ{KM7:TR?=	NImybJRUSU6JRWK=
HCC70	M2ftfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MnrjTWM2OD17LkK3N\|A6KM7:TR?=	MVjTRW5ITVJ?
MFH-ino	NWTnSlFET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MV\JR\|UxRTlwMkmwNFch\|ryP	M3fXO3NCVkeHUh?=
UACC-62	MmnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{XoTGlEPTB;OT6zO\|I6PyEQvF2=	NVnYS29JW0GQR1XS
C2BBe1	NHvrO\|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NEXQblRKSzVyPUmuN\|k3PTJizszN	M4X6OHNCVkeHUh?=
KNS-42	MknDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXzPZ3FXUUN3ME25MlQ{PDB5IN88US=>	NIDZepZUSU6JRWK=
SK-LMS-1	MoXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MorDTWM2OD17Lke1OVM2KM7:TR?=	M3u4WHNCVkeHUh?=
SK-CO-1	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmnXTWM2OD17Lke2NlM2KM7:TR?=	MVPTRW5ITVJ?
SN12C	MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEHJd4JKSzVyPUmuO\|Y5ODZizszN	NEnJ[HNUSU6JRWK=
HTC-C3	MlK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1PkemlEPTB;OT63O\|k5PCEQvF2=	NWCzVFlzW0GQR1XS
OVCAR-4	M4\NWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmDlTWM2OD17Lki0PFI5KM7:TR?=	MlzXV2FPT0WU
CHP-126	NUjZdZJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWXJR\|UxRTlwOUOyPUDPxE1?	NYfp[IpNW0GQR1XS
NCI-H716	NHzxXFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWjxSow4UUN3ME25Mlk{OzJ{IN88US=>	NUTmdVhqW0GQR1XS
U-698-M	NGrrZYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWfWPHV{UUN3ME25Mlk6PyEQvF2=	M2jFVHNCVkeHUh?=
EFO-21	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Ml;KTWM2OD1zMD6wNFk{KM7:TR?=	Mo\2V2FPT0WU
RXF393	NXzqPHFFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXTJR\|UxRTFyLkGzPVIh\|ryP	NFvGWIFUSU6JRWK=
ChaGo-K-1	M1[zb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MV\JR\|UxRTFyLkG3O\|Yh\|ryP	NWLnU5dJW0GQR1XS
SJRH30	MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2HOSGlEPTB;MUCuNVg1PCEQvF2=	NWDsdGUzW0GQR1XS
LU-65	M1zlc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NFHOc49KSzVyPUGwMlI6PDNizszN	NGHXVmhUSU6JRWK=
OS-RC-2	NF[wcVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUfMRW1sUUN3ME2xNE4{OjJ5IN88US=>	MUnTRW5ITVJ?
YT	M{i3fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYTCTZM5UUN3ME2xNE41ODBzIN88US=>	NHTBcYRUSU6JRWK=
COLO-679	M4e4XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWTJR\|UxRTFyLkSyPFUh\|ryP	NV\0PVBjW0GQR1XS
CAS-1	NGfiR5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MX7JR\|UxRTFyLkS2NFgh\|ryP	M1eyO3NCVkeHUh?=
NCI-H2052	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1jpXmlEPTB;MUCuOVExPSEQvF2=	MkDjV2FPT0WU
NCI-H2452	MkfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M17oN2lEPTB;MUCuOlE5OSEQvF2=	MkTNV2FPT0WU
SW13	NETjS49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NE\XNZdKSzVyPUGwMlY{PzRizszN	MkOyV2FPT0WU
HuP-T4	M4riSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYPxcI1uUUN3ME2xNE44ODZ6IN88US=>	MmfuV2FPT0WU
UMC-11	MmH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MV3JR\|UxRTFyLkmzO\|Mh\|ryP	MmjIV2FPT0WU
EGI-1	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYDJR\|UxRTFyLkm0OVUh\|ryP	M1PvfXNCVkeHUh?=
JiyoyeP-2003	NYC0VYI3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NELEfXRKSzVyPUGxMlA5QTJizszN	M3ezR3NCVkeHUh?=
J-RT3-T3-5	M2rOO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MV3JR\|UxRTFzLkGyNFkh\|ryP	MnPsV2FPT0WU
DoTc2-4510	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYixXFV4UUN3ME2xNU4yOzh7IN88US=>	NUi0doNvW0GQR1XS
HLE	NFfGOpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWHFUGd2UUN3ME2xNU4zOTF7IN88US=>	NEe0Xo9USU6JRWK=
SW626	Ml23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWLJR\|UxRTFzLkK4PFkh\|ryP	NHXmR25USU6JRWK=
COR-L105	NX3LR2FGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUHZW\|Y3UUN3ME2xNU4{OjN5IN88US=>	Mle4V2FPT0WU
ES6	NGW1fVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NV[zfZpxUUN3ME2xNU42PzZizszN	MknpV2FPT0WU
SNU-449	M{nXZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWnJR\|UxRTFzLk[wO\|Ih\|ryP	MlL3V2FPT0WU
GR-ST	MmXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVLqUmpNUUN3ME2xNU43OThzIN88US=>	MVnTRW5ITVJ?
BL-41	Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3XDXGlEPTB;MUGuO\|E4OyEQvF2=	NFjhOIxUSU6JRWK=
GB-1	M2W5W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{D6[WlEPTB;MUGuO\|YyQSEQvF2=	M{HuVnNCVkeHUh?=
COLO-320-HSR	NV3HfVl7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M37qbGlEPTB;MUGuPFUyQSEQvF2=	M3u4NXNCVkeHUh?=
L-540	NX3DSVl5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXXJR\|UxRTFzLki5PUDPxE1?	NULzU3dGW0GQR1XS
NCI-H69	Ml\qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFHveotKSzVyPUGyMlAyOzlizszN	M13E[3NCVkeHUh?=
769-P	NV7ISIlJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MYrJR\|UxRTF{LkCxPUDPxE1?	NY\yd2VNW0GQR1XS
A172	NEPQPG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Ml75TWM2OD1zMj6xNlA4KM7:TR?=	M4riVXNCVkeHUh?=
CAL-27	NYe3bHp1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUX2OFE4UUN3ME2xNk4yOzZ7IN88US=>	MWHTRW5ITVJ?
NCI-H1437	M4TY[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHe2[IZKSzVyPUGyMlE1PDdizszN	NUXGfWJZW0GQR1XS
REH	MlLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MoTGTWM2OD1zMj6xOVc{KM7:TR?=	MkPEV2FPT0WU
KINGS-1	NHXWVJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVLJb4FFUUN3ME2xNk4{PDJzIN88US=>	NVflT3BRW0GQR1XS
NCI-H1792	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVPJR\|UxRTF{LkO4OVEh\|ryP	NX7we3M{W0GQR1XS
SK-MM-2	Mo[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mk\XTWM2OD1zMj60NFg4KM7:TR?=	Mn;GV2FPT0WU
8505C	NXPze2hLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGi1cllKSzVyPUGyMlYyOiEQvF2=	MV7TRW5ITVJ?
HAL-01	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYrJR\|UxRTF{Lk[xO\|Qh\|ryP	M1XZbnNCVkeHUh?=
NCI-H2141	MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH3DSZZKSzVyPUGyMlY2QCEQvF2=	M{nKOnNCVkeHUh?=
HCC1806	NGD3UppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3TzSmlEPTB;MUKuOlk2OiEQvF2=	NIfaOIRUSU6JRWK=
LU-134-A	NUHqcGp{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHTub\|VKSzVyPUGyMlk{KM7:TR?=	MljmV2FPT0WU
HT	NHv5T5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MlT6TWM2OD1zMj65OFM4KM7:TR?=	NYPDfGNJW0GQR1XS
OE33	NVPEVXlXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2nqVGlEPTB;MUKuPVQ2PiEQvF2=	M4DBb3NCVkeHUh?=
ES4	MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NELNVlBKSzVyPUGyMlk1QDdizszN	MlWxV2FPT0WU
SW948	NGjsbm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MlnyTWM2OD1zMz6wNVMh\|ryP	NIGwbWlUSU6JRWK=
LB2241-RCC	NXXrXlFbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M4D1U2lEPTB;MUOuNVQ3QSEQvF2=	Moe0V2FPT0WU
CCF-STTG1	NH;aZohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHO3e5RKSzVyPUGzMlQxOzlizszN	MlizV2FPT0WU
RPMI-8402	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYHJR\|UxRTF\|LkWwN\|Eh\|ryP	MXrTRW5ITVJ?
DEL	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NX;Vd4V7UUN3ME2xN{42PTB\|IN88US=>	M1[xOXNCVkeHUh?=
NB5	NWfido5WT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2[yZmlEPTB;MUOuOVUxOyEQvF2=	NWDzT4FNW0GQR1XS
NUGC-3	NX\DeGNLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmnVTWM2OD1zMz63OFU{KM7:TR?=	MYHTRW5ITVJ?
CAL-72	M3HmfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWPJR\|UxRTF2LkG5NFYh\|ryP	NHLnS\|ZUSU6JRWK=
VMRC-RCZ	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn74TWM2OD1zND6yN\|Yh\|ryP	MUDTRW5ITVJ?
YAPC	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NIXQSmtKSzVyPUG0MlI1ODdizszN	MlO2V2FPT0WU
ES5	MmXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NWH3VY9xUUN3ME2xOE4zPTl7IN88US=>	NV3Se4pvW0GQR1XS
CAL-12T	M2DhTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXntUpBHUUN3ME2xOE41OzN4IN88US=>	MV\TRW5ITVJ?
GAMG	NIPnfG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MorYTWM2OD1zND61OVc4KM7:TR?=	M{C5VXNCVkeHUh?=
BT-20	M1jhWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4nMdGlEPTB;MUSuPVM5PCEQvF2=	MX7TRW5ITVJ?
KALS-1	M1[xVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mmn5TWM2OD1zNT6wNVU5KM7:TR?=	NFGz[Y1USU6JRWK=
LS-123	MkTIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NGXWelRKSzVyPUG1MlAyPjVizszN	MV7TRW5ITVJ?
LK-2	NGHtTXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MknkTWM2OD1zNT6wPFYzKM7:TR?=	Ml21V2FPT0WU
NCI-H1770	MmraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1zXU2lEPTB;MUWuNlg3QCEQvF2=	M1\DfXNCVkeHUh?=
MIA-PaCa-2	M1jIT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Ml\1TWM2OD1zNT60NVY{KM7:TR?=	M4K5XHNCVkeHUh?=
DB	MlTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mn;JTWM2OD1zNT62PVQyKM7:TR?=	NGi0VY1USU6JRWK=
Becker	MknoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3rmTGlEPTB;MUWuPFkzPiEQvF2=	M4fOcHNCVkeHUh?=
HT-144	NY\nVot2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2KybWlEPTB;MUWuPVQ6PyEQvF2=	MVTTRW5ITVJ?
NCI-H2405	M4L5bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVHwN\|VOUUN3ME2xOk4zOjF6IN88US=>	NGH5NoxUSU6JRWK=
TK10	MkHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{DFXmlEPTB;MU[uN\|c1OiEQvF2=	M4TKRXNCVkeHUh?=
THP-1	MnjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MojaTWM2OD1zNj60OlY2KM7:TR?=	M4THeXNCVkeHUh?=
LB373-MEL-D	NV;KbIRpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NX7aN3o5UUN3ME2xOk43OTN6IN88US=>	MVrTRW5ITVJ?
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NCI-H1355	NF3CdFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWrJSlMxUUN3ME2zOU44PzF6IN88US=>	M4L6cnNCVkeHUh?=
BV-173	M2DMbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGjpbFVKSzVyPUO1MlgzPjRizszN	M3zwcXNCVkeHUh?=
NEC8	M32we2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlzUTWM2OD1\|Nj60PVI1KM7:TR?=	MW\TRW5ITVJ?
SW1463	NYDSWWVmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXLJR\|UxRTN4Lk[1OlYh\|ryP	NFj6UWdUSU6JRWK=
KYSE-140	MnzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXH5XIR[UUN3ME2zOk44Ojl6IN88US=>	NVnLeGdVW0GQR1XS
no-11	Mo\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NICwOm5KSzVyPUO2Mlg2OzZizszN	MXnTRW5ITVJ?
HCC1937	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3;kU2lEPTB;M{euNFE2PCEQvF2=	NIHzZmVUSU6JRWK=
LNCaP-Clone-FGC	NUDa[IVLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUPCbmRxUUN3ME2zO{4yQTB\|IN88US=>	M{e3TXNCVkeHUh?=
KARPAS-422	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYq2eYNiUUN3ME2zO{41PDNzIN88US=>	NXrRU2xKW0GQR1XS
RCM-1	NIi5ZYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVHJR\|UxRTN5LkWzOVgh\|ryP	NFPO[GtUSU6JRWK=
A375	NGXtXWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWW4bI9sUUN3ME2zO{44PjR5IN88US=>	M1jBbXNCVkeHUh?=
TGBC24TKB	M1HWXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2PJdWlEPTB;M{iuNFI{QSEQvF2=	NGDB[nBUSU6JRWK=
MDA-MB-453	NU\DXIdrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NYPhS5ZxUUN3ME2zPU4xOjF6IN88US=>	NXfwcXQyW0GQR1XS
Capan-2	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NYXBeHdqUUN3ME2zPU4xPTB7IN88US=>	NVLxbJJUW0GQR1XS
SW756	NELzU\|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NIrWcHVKSzVyPUO5MlUxQTFizszN	M{XTc3NCVkeHUh?=
KU-19-19	NGnpc2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVrKPYljUUN3ME2zPU43Ojh{IN88US=>	NXjYcmhmW0GQR1XS
NCI-H2170	NE\j[IdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUf6VnpNUUN3ME2zPU46QTd5IN88US=>	NH\ENIdUSU6JRWK=
COLO-678	M1vmT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2PU[WlEPTB;NECuOVk2KM7:TR?=	M2PIeHNCVkeHUh?=
PF-382	MkTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlezTWM2OD12MD63OFg4KM7:TR?=	M1XKcHNCVkeHUh?=
OAW-42	NXf1dZpST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmfSTWM2OD12Mj6yPVQzKM7:TR?=	M2C4ZXNCVkeHUh?=
LB996-RCC	NVTGPFV2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHrHXIJKSzVyPUSyMlgxODhizszN	NIjYbGdUSU6JRWK=
SAS	NGHJToNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NEHRRllKSzVyPUSzMlQzQDhizszN	MVPTRW5ITVJ?
MRK-nu-1	M{fqWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHHvfYtKSzVyPUS0Mlc1OTRizszN	MnS4V2FPT0WU
EoL-1-cell	NHKzNmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1PPeWlEPTB;NESuPVE{PiEQvF2=	MljGV2FPT0WU
NCI-H2342	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1rZO2lEPTB;NEWuNVEyPyEQvF2=	MlXXV2FPT0WU
NCI-H1703	NFu2OGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NV3PTGN1UUN3ME20Ok4xPTl{IN88US=>	NFLSN3lUSU6JRWK=
NCI-H187	NXLrVlBIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHG4Wm5KSzVyPUS2MlE5PjlizszN	NF7Yc4dUSU6JRWK=
CP66-MEL	MmSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWjJR\|UxRTR4LkK3PFkh\|ryP	NHKzNFlUSU6JRWK=
HCC1569	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M136T2lEPTB;NE[uOVkyPyEQvF2=	NXjGdo51W0GQR1XS
BL-70	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1jObmlEPTB;NE[uO\|c{PyEQvF2=	M2XuWnNCVkeHUh?=
Saos-2	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1vM[WlEPTB;NEeuN\|EzOyEQvF2=	NELhWIJUSU6JRWK=
LC-1F	M4HLbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWrJR\|UxRTR5Lk[xNFUh\|ryP	MXTTRW5ITVJ?
KY821	M4fnW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1TjcmlEPTB;NEeuOlQ{PCEQvF2=	NWrhd2hjW0GQR1XS
PSN1	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoLoTWM2OD12OD6xOFAyKM7:TR?=	MVPTRW5ITVJ?
RT4	NGDGRnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NF;FbY5KSzVyPUS4Mlg{PzdizszN	MonnV2FPT0WU
LS-1034	NVi0TIpZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVH5Wot7UUN3ME20PE45QTV2IN88US=>	MXHTRW5ITVJ?
NCI-H1395	NUSzR\|A1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUnhUXg2UUN3ME20PU4xPDl{IN88US=>	Mmj4V2FPT0WU
EKVX	NV65cFMxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXfIOm8yUUN3ME20PU43PzNzIN88US=>	MlyzV2FPT0WU
IST-MES1	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVTJR\|UxRTR7Lki5OFQh\|ryP	NEW0TZBUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo

PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. ^[1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. ^[3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. ^[4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. ^[5]

Protocol

Animal Research:^[1]	+ Expand Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts. Formulation: 5% Gelucire Dosages: 50 mg/kg Administration: Oral gavage (Only for Reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	100 mg/mL warmed (197.04 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 30% PEG400+0.5% Tween80+5% propylene glycol For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download PF-562271 SDF

Molecular Weight	507.49
Formula	C₂₁H₂₀F₃N₇O₃S
CAS No.	717907-75-0
Storage	3 years -20°C powder
Storage	2 years -80°C in solvent
Synonyms	N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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FAK Signaling Pathway Map

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Defactinib (VS-6063, PF-04554878) ^New

Defactinib (VS-6063, PF-04554878) is a selective, and orally active FAK inhibitor. Phase 2.
PF-00562271 Besylate

PF-00562271 Besylate is the benzenesulfonate salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.
TAE226 (NVP-TAE226)

TAE226 (NVP-TAE226) is a potent FAK inhibitor with IC50 of 5.5 nM and modestly potent to Pyk2, ~10- to 100-fold less potent against InsR, IGF-1R, ALK, and c-Met.
PF-573228

PF-573228 is an ATP-competitive inhibitor of FAK with IC50 of 4 nM in a cell-free assay, ~50- to 250-fold selective for FAK than Pyk2, CDK1/7 and GSK-3β.
PF-562271 HCl

PF-562271 HCl is the hydrochloride salt of PF-562271, which is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs. Phase 1.
Ibrutinib (PCI-32765)

Ibrutinib (PCI-32765) is a potent and highly selective Brutons tyrosine kinase (Btk) inhibitor with IC50 of 0.5 nM in cell-free assays, modestly potent to Bmx, CSK, FGR, BRK, HCK, less potent to EGFR, Yes, ErbB2, JAK3, etc.
Dasatinib

Dasatinib is a novel, potent and multi-targeted inhibitor that targets Abl, Src and c-Kit, with IC50 of <1 nM, 0.8 nM and 79 nM in cell-free assays, respectively.
Saracatinib (AZD0530)

Saracatinib (AZD0530) is a potent Src inhibitor with IC50 of 2.7 nM in cell-free assays, and potent to c-Yes, Fyn, Lyn, Blk, Fgr and Lck; less active for Abl and EGFR (L858R and L861Q). Phase 2/3.

Features:The 1st Src inhibitor to show inhibition of the Src pathway in human tumor tissue.

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PF-562271