PF-562271

Catalog No.S2890

PF-562271 Chemical Structure

Molecular Weight(MW): 507.49

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.

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In DMSO USD 350 In stock
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USD 270 In stock
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Cited by 14 Publications

6 Customer Reviews

  • Effect of matrix stiffness on the susceptibility of primary HDFs to apoptosis induced by inhibition of mechanotransduction pathways. HDFs (primary human dermal fibroblasts) were cultured on collagen-coated polyacrylamide hydrogels that recapitulated the stiffness of normal (4 kPa) or densely fibrotic (50 kPa) skin for 24 hours and treated with or without the agents indicated for an additional 48 hours. Apoptosis was assessed by annexin V staining. Data are means ± SD from three independent experiments. P value was determined by Student’s t test.

    Science, 2017, 9(420), doi: 10.1126/scitranslmed.aal3765. PF-562271 purchased from Selleck.

    (F,G) The migration and invasion ability of 5637 and T24 cells transfected with PF-562271 and WT-METTL13 was examined. The number of cells was quantified and is shown in a column graph.

    Sci Rep, 2016, 6:19261. PF-562271 purchased from Selleck.

  • D-F. T47D cells plated as in A were treated with vehicle (−) or the FAK Y397 inhibitor, PF-562271 (+), for 1 h prior to ± PRL (4 nM) for 15 min (representative immunoblots).

    Oncotarget, 2016, 7(30):48093-48106. PF-562271 purchased from Selleck.

    Acta Pharmacol Sin, 2018, doi:10.1038/aps.2017.189. PF-562271 purchased from Selleck.

  • VEGF and P450 enzyme production in response to FAK or Src inhibitors. MCF10A cells were cultured in LD collagen gels ?vehicle control (V), 10 uM PF-562271 FAK ATP inhibitor (PF) or Src inhibitor PP2 for 24 h. Cells were also lysed and examined for CYP1A1 or CYP4B1 by immunoblot (B and C) and densitometry (D and E). VEGF data are displayed as the mean normalized to the vehicle control, N = 6, 盨EM, *p < 0.003 vs control (A). P450 data are displayed as the mean relative to GAPDH and normalized to the control. N > 4, ±SEM, *p < 0.05 vs respective control (D and E).

    Environ Toxicol Pharmacol 2014 39(1), 114-124. PF-562271 purchased from Selleck.

    OVISE cells were incubated for 24 hr at the indicated concentrations of the FAK inhibitors. Immunoblots were performed to assess inhibition of auto-phosphorylation by the FAK inhibitors.

    PLoS One 2014 9(2), e88587. PF-562271 purchased from Selleck.

Purity & Quality Control

Choose Selective FAK Inhibitors

Biological Activity

Description PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK with IC50 of 1.5 nM in cell-free assays, ~10-fold less potent for Pyk2 than FAK and >100-fold selectivity against other protein kinases, except for some CDKs.
Targets
FAK [1]
(Cell-free assay)
PYK2 [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
CDK3/CyclinE [1]
(Cell-free assay)
CDK1/CyclinB [1]
(Cell-free assay)
1.5 nM 13 nM 30 nM 47 nM 58 nM
In vitro

PF-562271 binds in the ATP-binding cleft of FAK, forming two of the three “canonical” H-bonds between the inhibitor and main-chain atoms in the kinase hinge region. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with IC50 of 5 nM. PF-562271 (3.3 μM) results in G1 arrest of PC3-M cells. [1] PF-562271 (1 nM) blocks bFGF-stimulated blood vessel angiogenesis as performed in chicken chorioallantoic membrane assays. PF-262271 potently blocks blood vessel sprouting without detectable changes in vascular leakage. [2] PF-562271 (250 nM) results in complete inhibition of collective A431 cell invasion into collagen gels. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MV-4-11 NIDhfo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTBwMke2OkDPxE1? NVTzT4lxW0GQR1XS
SW982 NYXjemdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfLTWM2OD1yLkOyPFIh|ryP NYG5emVCW0GQR1XS
KM12 NVziTZVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTBwM{i1OVch|ryP M3:0SXNCVkeHUh?=
COLO-205 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nI[2lEPTB;MD60PFY2QCEQvF2= M{HnOHNCVkeHUh?=
COLO-829 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPYb29KSzVyPUCuO|UyPzZizszN NIOxUoZUSU6JRWK=
MG-63 NWf3eWFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvCZlNKSzVyPUCuPFA3OzdizszN NXrYcZpVW0GQR1XS
IGROV-1 M{LjUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NET6Z2tKSzVyPUCuPFExOzhizszN MnHXV2FPT0WU
NCI-H650 M3XKOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHqwWJNKSzVyPUCuPFMyPTRizszN M2D2OHNCVkeHUh?=
RT-112 M{HLbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGP1c5VKSzVyPUCuPVg1PiEQvF2= MVTTRW5ITVJ?
BCPAP Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVi4SHlHUUN3ME2xMlAyOjh6IN88US=> NEfIZ4RUSU6JRWK=
ALL-PO M4XXdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnYTWM2OD1zLkCxOVg1KM7:TR?= NH3BOmFUSU6JRWK=
KYSE-270 NH[5dJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHaNWtKSzVyPUGuNFQ4OTRizszN M4TzR3NCVkeHUh?=
8305C NFLqdXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoO5TWM2OD1zLkC5PVA1KM7:TR?= NYDrN5BiW0GQR1XS
NCI-H810 NWTHXG5ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXTS4ZmUUN3ME2xMlExPzd4IN88US=> MUTTRW5ITVJ?
CAL-33 NVTxSoFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnUcoFKSzVyPUGuNVI6OzhizszN MnXiV2FPT0WU
AN3-CA MoXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTFwMkG4Olch|ryP NEjLbFNUSU6JRWK=
NKM-1 M13yU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jucWlEPTB;MT6yO|UxPiEQvF2= NETKU4NUSU6JRWK=
BPH-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTFwMki3OlYh|ryP MV7TRW5ITVJ?
MES-SA MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mni5TWM2OD1zLkOwOlgzKM7:TR?= MVzTRW5ITVJ?
CAL-62 NFXxN|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33uPGlEPTB;MT6zNVkxQSEQvF2= NXXzXnc1W0GQR1XS
Detroit562 NV:1R|dNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHP4UHFKSzVyPUGuN|UzOjJizszN MVPTRW5ITVJ?
KYSE-150 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[z[nFFUUN3ME2xMlM2OjN4IN88US=> NIr3[YFUSU6JRWK=
SK-UT-1 M4TUUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETtOYRKSzVyPUGuOFQ3PDdizszN MmnVV2FPT0WU
HUTU-80 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13pT2lEPTB;MT60OFg5PiEQvF2= NWThZlY3W0GQR1XS
SIG-M5 Ml2wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTFwNEizPFch|ryP MlrjV2FPT0WU
AGS M2Hqdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorWTWM2OD1zLkWyNVI1KM7:TR?= M4rtZ3NCVkeHUh?=
ST486 MmfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDtO3lSUUN3ME2xMlU{Ojd6IN88US=> MXnTRW5ITVJ?
HSC-2 M37MNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrOV2FKSzVyPUGuOVM6PSEQvF2= NWnTenljW0GQR1XS
LCLC-103H MkXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTFwNUW4NVgh|ryP MmnyV2FPT0WU
BC-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTFwNkG2OlQh|ryP M1vleXNCVkeHUh?=
CGTH-W-1 M3XSe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTFwNkG2O|kh|ryP MX;TRW5ITVJ?
MZ1-PC NVuxfnpGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfhTWM2OD1zLk[yN|EzKM7:TR?= NFvPelBUSU6JRWK=
SW1710 NYrVZ3FtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTFwNkK2Nlgh|ryP NFLmUnZUSU6JRWK=
EW-13 M1[0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTFwNkO0OlYh|ryP M3PXNHNCVkeHUh?=
TE-11 NIjjZWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTFwNkO1NlIh|ryP MonzV2FPT0WU
BHY MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTIW3ZbUUN3ME2xMlY2PjZ|IN88US=> NIHlfINUSU6JRWK=
ML-2 NVnqUGxpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYSxXnhnUUN3ME2xMlY3QDB{IN88US=> M3LBbHNCVkeHUh?=
MEL-JUSO MoLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\idHQyUUN3ME2xMlczQDlzIN88US=> NX;XOVduW0GQR1XS
U251 NXj5fHl{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjRTWM2OD1zLke0NFMyKM7:TR?= NGfrOpBUSU6JRWK=
MHH-ES-1 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4P3UWlEPTB;MT63PVU3QCEQvF2= M2W5VnNCVkeHUh?=
CAL-85-1 MoCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYj2bm9VUUN3ME2xMlgxPDlizszN MYrTRW5ITVJ?
D-336MG Mo\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TiOGlEPTB;MT64OVYyPCEQvF2= M3jjbHNCVkeHUh?=
VA-ES-BJ MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnuxTWM2OD1zLkmwN|E2KM7:TR?= NWLBZ2dqW0GQR1XS
MDA-MB-468 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnmwTWM2OD1zLkmxNlczKM7:TR?= M{D5dXNCVkeHUh?=
MEL-HO NHjhSYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TCeGlEPTB;MT65O|k6OyEQvF2= NH7mWo5USU6JRWK=
SW954 NIjGemxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7G[Wo1UUN3ME2yMlAxOTVzIN88US=> M{[1WHNCVkeHUh?=
LB831-BLC M1HQNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEfkZ4dKSzVyPUKuNFA6QDhizszN NFvhU|dUSU6JRWK=
EW-16 Ml\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTJwMEO5PFMh|ryP NX:3fWpKW0GQR1XS
NCI-H460 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTJwMES4N|kh|ryP NYj4PIFiW0GQR1XS
CHP-212 NGLoVoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTJwMEm1N|kh|ryP NHL0fpZUSU6JRWK=
MHH-NB-11 NED1cm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXMPWFbUUN3ME2yMlEzQDF6IN88US=> NWfLfmZiW0GQR1XS
DU-4475 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrQfmJMUUN3ME2yMlE1PzV7IN88US=> M{LtUnNCVkeHUh?=
ABC-1 M2X0N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHUTWM2OD1{LkK1NVk3KM7:TR?= MnTtV2FPT0WU
LOUCY NF\vNYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XCS2lEPTB;Mj6yOlEzQCEQvF2= M2HCeXNCVkeHUh?=
LC-2-ad MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\COGJuUUN3ME2yMlI3QTZ4IN88US=> NYHUOoJwW0GQR1XS
ETK-1 NV;vdoV3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rrV2lEPTB;Mj6yPFQ3KM7:TR?= M1TTO3NCVkeHUh?=
KYSE-180 M1q5WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW0TWM2OD1{LkK4PFc1KM7:TR?= M1LyUXNCVkeHUh?=
HOP-92 NIDOcYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWj5fVNvUUN3ME2yMlMxPjd|IN88US=> MXjTRW5ITVJ?
RCC10RGB MoXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTJwM{K1NFch|ryP NYSzWmJPW0GQR1XS
KASUMI-1 NUnQbo9tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnsTWM2OD1{LkOyPFgh|ryP MmGwV2FPT0WU
ACN NGju[XJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XZOWlEPTB;Mj6zOFc1PSEQvF2= NIPT[|lUSU6JRWK=
BxPC-3 M1f1b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;ENFVKSzVyPUKuN|U1OzZizszN MmG0V2FPT0WU
MONO-MAC-6 NI\TOGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGn4eWpKSzVyPUKuOFEzPDhizszN MX\TRW5ITVJ?
CAL-39 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvwTWM2OD1{LkSxOlI5KM7:TR?= NWXDfY9lW0GQR1XS
CMK NVqxco1[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXWTWM2OD1{LkSxO|k2KM7:TR?= MkDmV2FPT0WU
MFE-296 NX7QcmZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\hOo5KSzVyPUKuOFc4QTJizszN MX;TRW5ITVJ?
DU-145 M1L5NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\ETWM2OD1{LkS5NVE5KM7:TR?= NF7hfIhUSU6JRWK=
MDA-MB-231 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn25TWM2OD1{LkS5OVczKM7:TR?= M1zLWnNCVkeHUh?=
EFO-27 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTJwNUG4PFgh|ryP MVXTRW5ITVJ?
SNU-387 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTjWW9KSzVyPUKuOVI5OiEQvF2= NEHJOo1USU6JRWK=
KYSE-410 NV\CZY1yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTJwNU[1NVch|ryP M1HweXNCVkeHUh?=
NCCIT NXTiUHZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPObXJKSzVyPUKuOVczPCEQvF2= NUjlc2lyW0GQR1XS
LXF-289 NGjw[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTJwNUiyO|Mh|ryP NHnBSGFUSU6JRWK=
HMV-II NX7Yb|NlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjyTWM2OD1{LkW4PVk4KM7:TR?= MYrTRW5ITVJ?
MOLT-4 MonFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\ETWM2OD1{Lk[wO|EzKM7:TR?= MojwV2FPT0WU
KOSC-2 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTJwNkO4O|kh|ryP MUfTRW5ITVJ?
TUR NGm0cFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33CXGlEPTB;Mj62OVM3QSEQvF2= M2SzeXNCVkeHUh?=
NCI-H2087 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHGTWM2OD1{LkexOlQ3KM7:TR?= MkHoV2FPT0WU
SCC-15 NHzFOnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\0TWM2OD1{Lke1NlUzKM7:TR?= MYrTRW5ITVJ?
HSC-4 NIjLNo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnnSZBKSzVyPUKuO|g{QDlizszN Mm\ZV2FPT0WU
G-402 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7VbYMzUUN3ME2yMlgyPDR5IN88US=> MlLQV2FPT0WU
GP5d MorQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\mRmlEPTB;Mj64N|gzPyEQvF2= NGDPXIVUSU6JRWK=
IGR-1 Ml\TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3PfmtKSzVyPUKuPFM6QDdizszN Mni3V2FPT0WU
SK-PN-DW M1Lmb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPDTWM2OD1{Lki2NlA1KM7:TR?= Mn[1V2FPT0WU
OPM-2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTJwOEm5O|gh|ryP Ml31V2FPT0WU
5637 M{PvdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnn4TWM2OD1{LkmyOFM6KM7:TR?= MkDQV2FPT0WU
NCI-H2030 MlG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrHTWM2OD1{Lkm4NFI6KM7:TR?= NGnzXJBUSU6JRWK=
SK-LU-1 MkDjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTJwOUizN|Qh|ryP MVfTRW5ITVJ?
EW-18 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTWTWM2OD1|LkC1N|Ih|ryP M1zkTHNCVkeHUh?=
786-0 NF2ybINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTNwMEW3PFQh|ryP M37DenNCVkeHUh?=
NCI-H1963 M1jaXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIL5bI1KSzVyPUOuNFg5OjVizszN MWXTRW5ITVJ?
A2780 NWHabXJJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU[0ZpR6UUN3ME2zMlExOzRzIN88US=> M1zPSHNCVkeHUh?=
NCI-H526 MoXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETufG5KSzVyPUOuNVM6PjFizszN NHPoSZVUSU6JRWK=
BHT-101 Mk\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTNwMUW3PVgh|ryP MoS1V2FPT0WU
RPMI-7951 NVPFdIJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIr3N3VKSzVyPUOuNlAyOzdizszN NVq2VVdtW0GQR1XS
HL-60 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjtdGpHUUN3ME2zMlIyOTRizszN NVjmSHU{W0GQR1XS
CTV-1 MljCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInyPVZKSzVyPUOuNlU{PzdizszN M4r2Z3NCVkeHUh?=
IST-SL1 NFXBXHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjMZ|hWUUN3ME2zMlI6PDZ3IN88US=> MonUV2FPT0WU
G-401 M1znSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzSVmRzUUN3ME2zMlI6QDB6IN88US=> MXnTRW5ITVJ?
OVCAR-5 NUDpO2M5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;4TpNnUUN3ME2zMlM3OzV{IN88US=> NF7rd2VUSU6JRWK=
EW-1 NYe3WG52T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LqfmlEPTB;Mz60NFU1PCEQvF2= MmTGV2FPT0WU
H4 NHTxVItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTNwNEe1Olkh|ryP NUO1ZpY{W0GQR1XS
EM-2 Mk\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDpTnEyUUN3ME2zMlQ5Pjd2IN88US=> NH;XWnlUSU6JRWK=
RS4-11 NVr3ZnpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTNwNEi3NVUh|ryP NF;5SIJUSU6JRWK=
NOMO-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETubm1KSzVyPUOuOFkzODRizszN MmnYV2FPT0WU
HCT-116 NFrCfVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zCZ2lEPTB;Mz61NFcyOSEQvF2= NEi1XFdUSU6JRWK=
CAL-120 MkiwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIradZhKSzVyPUOuOVc{PSEQvF2= NWTTfHN4W0GQR1XS
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COLO-678 NUXJVphqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTRyLkW5OUDPxE1? Mk\3V2FPT0WU
PF-382 M2qxcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTRyLke0PFch|ryP M1;TSnNCVkeHUh?=
OAW-42 M3L2PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTR{LkK5OFIh|ryP MUfTRW5ITVJ?
LB996-RCC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXjTWM2OD12Mj64NFA5KM7:TR?= MWTTRW5ITVJ?
SAS NIntOFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfyNmdKSzVyPUSzMlQzQDhizszN M4PZS3NCVkeHUh?=
MRK-nu-1 NYO1cWxGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTR2Lke0NVQh|ryP NU[0PYR6W0GQR1XS
EoL-1-cell M2DBfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHP3d3JKSzVyPUS0MlkyOzZizszN MXLTRW5ITVJ?
NCI-H2342 M1Owb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnToTWM2OD12NT6xNVE4KM7:TR?= MU\TRW5ITVJ?
NCI-H1703 M1vwZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\6fJFKSzVyPUS2MlA2QTJizszN NH;TcohUSU6JRWK=
NCI-H187 MlrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTR4LkG4Olkh|ryP MUXTRW5ITVJ?
CP66-MEL M1L0O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTR4LkK3PFkh|ryP NIXCN|RUSU6JRWK=
HCC1569 NXHTco1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3S0WWlEPTB;NE[uOVkyPyEQvF2= MkmyV2FPT0WU
BL-70 MlTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTR4Lke3N|ch|ryP NEPjd|VUSU6JRWK=
Saos-2 NIGxS3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;1clFKSzVyPUS3MlMyOjNizszN NF\CZYNUSU6JRWK=
LC-1F NILiR4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;Ub2lEPTB;NEeuOlExPSEQvF2= NImxUXpUSU6JRWK=
KY821 MmLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnDRZVoUUN3ME20O{43PDN2IN88US=> NGjRNZpUSU6JRWK=
PSN1 NYDkOJl{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofvTWM2OD12OD6xOFAyKM7:TR?= MV;TRW5ITVJ?
RT4 NGTKS5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPzZnVpUUN3ME20PE45Ozd5IN88US=> MVXTRW5ITVJ?
LS-1034 NIjwdlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHGdIRzUUN3ME20PE45QTV2IN88US=> MUXTRW5ITVJ?
NCI-H1395 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojtTWM2OD12OT6wOFkzKM7:TR?= M{DPbHNCVkeHUh?=
EKVX NF76d4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTzTWM2OD12OT62O|MyKM7:TR?= NYfEdJNkW0GQR1XS
IST-MES1 MmLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTR7Lki5OFQh|ryP M3qxS3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo PF-562271 (< 33 mg/kg p.o.) inhibits FAK phosphorylation in tumors in a dose- and time-dependent manner in U87MG-bearing mice. PF-562271 (50 mg/kg p.o. bid) results in 86% tumor growth inhibition in BxPc3 xenografts mice and 45% tumor growth inhibition in PC3-M xenografts mice. PF-562271 (25 mg/kg, bid) results in 2-fold greater apoptosis in treated tumors in mice bearing H125 lung xenografts. [1] PF-562271 (33 mg/kg, p.o.) inhibits extensive movement of the tumor cells over 24 hours in mice. PF-562271 (33 mg/kg, p.o.) results in altered E-cadherin dynamics in mice, which correlates with reduced E-cadherin–dependent collective cell movement. [3] PF-562271 (25 mg/kg, p.o. bid) results in 62% tumor growth inhibition in PC3M-luc-C6 subcutaneuous local implant xenograft mouse model. [4] PF-562,271 (5 mg/kg, oral) results in significant and similar increases in osteocalcin and cancellous bone parameters and a decrease in tumor growth and signs of bone healing in rats implanted with MDA-MB-231 cells in the tibia. [5]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Athymic female mice bearing BxPc3 or PC3-M xenografts.
  • Formulation: 5% Gelucire
  • Dosages: 50 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (197.04 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.49
Formula

C21H20F3N7O3S

CAS No. 717907-75-0
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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FAK Signaling Pathway Map

Related FAK Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID