Name: GW3965 HCl
Brand: Selleck Chemicals
SKU: S2630
Rating: 5 (32 reviews)

CAS No. 405911-17-3

Selleck's GW3965 HCl has been cited by 32 publications

Purity & Quality Control

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Biological Activity

Description

GW3965 HCl is a potent, selective LXR agonist for hLXRα and hLXRβ with EC50 of 190 and 30 nM in cell-free assays, respectively.

Targets

hLXRβ ^[1] (Cell-free assay)	LXRα/SRC1 LiSA ^[1] (Cell-free assay)	hLXRα ^[1] (Cell-free assay)
30 nM(EC50)	125 nM(EC50)	190 nM(EC50)

In vitro

GW3965 recruits the steroid receptor coactivator 1 to human LXRα with EC50 of 125 nM in a cell-free ligand-sensing assay. ^[1] GW3965 shows a potent antagonistic activity against hLXRα and hLXRβ in cell-based assays with EC50 of 190 nM and 30 nM, respectively. Besides, GW3965 also sows excellent selectivity over other nuclear receptors. ^[1] In human islets, GW3965 (1 μM) reduces expression of selected pro-inflammatory cytokines including IL-8, monocyte chemotactic protein-1 and tissue factor. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HEK293		M4O5NVExKHWP	NVOwU3NWOjBiaILz	MkXmRYN1cX[jdHnvckBw\iCqdX3hckBNYFKjbIDoZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5NqdmdiaIXtZY4hWliUYXzwbIEh[XRiMUCgeW0h[W[2ZYKgNlAhcHK\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDByNkmwPUc,OjhyME[5NFk9N2F-
HEK293		Mlr5NVAhfU1?	M3TwR\|IxKGi{cx?=	M4TTfGFkfGm4YYTpc44hd2ZicnH0JGxZWmKndHGg[ZhxemW\|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGi3bXHuJHJZWmGucHjhJIF1KDFyIIXNJIFnfGW{IEKwJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	M4nrOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEC2PVA6Lz5{OECwOlkxQTxxYU6=
THP1	M2f1N2Z2dmO2aX;uJIF{e2G7			NF;YeG1KdmS3Y4Tpc44hd2ZiY3jvcIV{fGW{b3yg[YZndHW6IHnuJHRJWDFiY3XscJMtKEWFNUCgQUAxNjBzIN88UU4>	NWnGd\|ZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFc2PzNpPkG3OVg4PTd\|PD;hQi=>
THP1	NWS4[Vh{TnWwY4Tpc44h[XO\|YYm=		MkHSNVghcHK\|	MXrJcoR2[3Srb36gc4Yh[2ixbHXzeIVzd2xiZX\mcJV5KGmwIGTIVFEh[2WubIOgZYZ1\XJiMUigbJJ{NCCHQ{WwJF0hOC5yMTFOwG0v	M4XIVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG2OVIyLz5zN{SxOlUzOTxxYU6=
COS7	MoTCSpVv[3Srb36gZZN{[Xl?		NV61WFdTOTZiaILz	M3i3emFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gUHhT[mW2YTDy[YNmeHSxcjD0doFve2[nY4Tl[EBqdiCFT2O3JINmdGy\|IHHmeIVzKDF4IHjyd{BjgSC{ZYDvdpRmeiC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAyPSEQvF2u	M{PaZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi3OVc{Lz5zN{W4O\|U4OzxxYU6=
COS7	NHzwdHlHfW6ldHnvckBie3OjeR?=			MmDVRYN1cX[jdHnvckBw\iCOWGLi[ZRiKGOxLYTyZY5{\mWldHXkJIlvKEORU{egZ4VtdHNid3n0bEBTYFKjbIDoZUBjgSC{ZYDvdpRmeiC2cnHud4FkfGm4YYTpc44h[XO\|YYmsJGVEPTBiPTCwMlAyPSEQvF2u	NHThNmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxOlUzOSd-MUe0NVY2OjF:L3G+
THP1	NYTkSIVlSW62aXnu[oxidW2jdH;yfUBie3OjeR?=		NELj[pk3KGi{cx?=	M1HMS2FvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCWSGCxJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhUUx4IIDyc4R2[3Srb36gZYZ1\XJiNjDodpMh[nliRVzJV2EtKEmFNUCgQUAxNjB{IN88UU4>	NXLveYdkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NFA4PjdpPkG4PFAxPzZ5PD;hQi=>
THP1	M4CzfGZ2dmO2aX;uJIF{e2G7			MmS3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBISUxvbHnub4VlKGi3bXHuJGxZWmKndHGg[ZhxemW\|c3XkJIlvKFSKUEGgZ4VtdHNiYYPz[ZN{\WRiYYOgd5RqdXWuYYTpc44hd2ZiY3:tZYN1cX[jdH;yJJJm[3K3aYTt[Y51KGK7IF\SSXQh[XO\|YYmsJGVEPTBiPTCwMlAzPyEQvF2u	M3nON\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[1PFk4Lz5zN{[2OVg6PzxxYU6=
RAW264.7	MlXlSpVv[3Srb36gZZN{[Xl?		NV:yNJpvOjRiaILz	Mm\kTY5lfWO2aX;uJI9nKFt\|SG3jbI9t\XO2ZYLvcEBm\m[udYigbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNibH;h[IVlKHerdHigZYNmfHmuYYTl[E1NTExiYX\0[ZIhOjRiaILzMEBGSzVyIE2gNE4xOjlizszNMi=>	M1:wfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{G3N\|A1Lz5zOUexO\|MxPDxxYU6=
THP1	Mk\jSpVv[3Srb36gZZN{[Xl?			Mo\HV5RqdXWuYYTpc44hd2ZiW{PIYYNpd2ync4Tldo9tKGWoZnz1fEBqdiCqdX3hckBVUFBzIH\vZY0h[2WubIOgcI9i\GWmIIfpeIgh[WNvTFTMMEBGSzVyIE2gNE4xOzFizszNMi=>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl5M{K4PEc,OTh7N{OyPFg9N2F-
CV1	Mn64SpVv[3Srb36gZZN{[Xl?			M{fFSmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiTGjSZoV1[SCuaXfhcoQh[mmwZHnu[{Bld22jaX6gZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDUNVMyPy2rbnT1Z4VlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDDWlEh[2WubIOgZ48ufHKjboPm[YN1\WRid3n0bEBI[Wx2LWPSR\|Eh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDhd5NigSxiSVO1NEA:KDBwMEO5PFEh\|ryPLh?=	M1yzUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{S1NVAzLz5{MEO0OVExOjxxYU6=
THP1	NHT2fGdHfW6ldHnvckBie3OjeR?=			NGjDNFRC\2:waYP0JIFkfGm4aYT5JIF1KEeDTD3sbY5s\WRiaIXtZY4hVFiUYXzwbIEh\XiycnXzd4VlKGmwIGTIVFEh[2WubIOgZZN{\XO\|ZXSgZZMhe3SrbYXsZZRqd25ib3[gZ49i[3SrdnH0c5IhemWlcoXpeI1mdnRiYomgSnJGXCCjc4PhfUwhTUN3MDC9JFAvODl5IN88UU4>	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4NUi5O{c,OTd4NkW4PVc9N2F-
CV1	NILLVFRHfW6ldHnvckBie3OjeR?=			NVvqfpl1SW62YXfvcol{fCCjY4Tpeol1gSCjdDDMXHJidHCqYTDsbYdidmRiYnnu[Ilv\yCmb33hbY4h[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBVOTNzNz3pcoR2[2WmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCrbjDh[pJq[2GwIHfy[YVvKG2xbnvlfUBEXjFiY3XscJMh[29vdILhcpNn\WO2ZXSge4l1cCCJYXy0MXNTSzFiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeTygTWM2OCB;IECuNUDPxE1w	NXXo[4RoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOFUyODJpPkKwN\|Q2OTB{PD;hQi=>
SH-SY5Y	Mn;xSpVv[3Srb36gZZN{[Xl?		NGjXTlYzPCCqcoO=	MkX3RYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDMXHJj\XSjIHX4dJJme3OnZDDpckBpfW2jbjDTTE1UYTW\IHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR3HsOE1NSkRiYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNVMh\|ryPLh?=	MlSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{NkS0PFEoRjF7Mk[0OFgyRC:jPh?=
HepG2	NYnVS2xbTnWwY4Tpc44h[XO\|YYm=			Mnv4SYZn\WO2IH;uJHNTTUKSMXOg[4Vv\SCneIDy[ZN{cW:wIHnuJIh2dWGwIFjldGczKGOnbHzzMEBGSzVyIE2gNE4zOSEQvF2u	MlrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7N{OyPFgoRjF6OUezNlg5RC:jPh?=
HuH7	MnvnSpVv[3Srb36gZZN{[Xl?			NYXNcIlqSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBz\WOxbXLpcoFvfCCOWGLi[ZRiKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDpckBpfW2jbjDIeWg4KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIgh\nW\|ZXSgS4FtPC2GQlSgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD6zNUDPxE1w	MojsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7N{OyPFgoRjF6OUezNlg5RC:jPh?=
SH-SY5Y	MUXGeY5kfGmxbjDhd5NigQ>?		NXfRUYlJOjRiaILz	MkfERYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDMXHJidHCqYTDlfJBz\XO\|ZXSgbY4hcHWvYX6gV2guW1l3WTDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIFfhcFQuVEKGIHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYmsJGVEPTBiPTCwMlMyKM7:TT6=	NYXQZ292RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmyOlQ1QDFpPkG5NlY1PDhzPD;hQi=>
CHO	M1Wxe2Z2dmO2aX;uJIF{e2G7			MkfmRYdwdmm\|dDDhZ5Rqfmm2eTDheEBpfW2jbjDMXHIh[mW2YTDy[YNmeHSxcjDlfJBz\XO\|ZXSgbY4hS0iRIHPlcIx{KGK7IILldI9zfGW{IHHzd4F6NCCHQ{WwJF0hOC52MTFOwG0v	NF7vOWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CzOFEyQSd-MUewN\|QyOTl:L3G+
CHOK1	NEG5dIVHfW6ldHnvckBie3OjeR?=		NEfadGUzPCCqcoO=	NUPCVXN3SWexbnnzeEBi[3Srdnn0fUBifCCJYXy0MZRi\2enZDDMXHJj\XSjIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGNJV0tzIHPlcIx{KGGodHXyJFI1KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVAhRSByLkSyJO69VS5?	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ5N{[2OEc,OjV4N{e2OlQ9N2F-
THP1	NYP0O5dSTnWwY4Tpc44h[XO\|YYm=			M2jn[mVn\mWldDDvckBCSkODMTDn[Y5mKGW6cILld5Nqd25iaX6gbJVu[W5iZHnm[oVz\W62aXH0[YQhXEiSMTDj[YxteyxiRVO1NEA:KDBwNEO0JO69VS5?	NEDaVoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm3N\|I5QCd-MUi5O\|MzQDh:L3G+
CV1	M{P2TWZ2dmO2aX;uJIF{e2G7			NYf6d5dySWexbnnzeEBi[3Srdnn0fUBifCCOWGLi[ZRiKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbj3t[YRq[XSnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS1ZzIHPlcIx{KGOxLYTyZY5{\mWldHXkJJdqfGhiR3HsOE1UWkNzIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh[XO\|YYmsJGVEPTBiPTCwMlUxOTF7IN88UU4>	NE\OcVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEO0OVExOid-MkCzOFUyODJ:L3G+
HuH7	NHS3VIRHfW6ldHnvckBie3OjeR?=			NGnxbGRC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEy[UnHsdIhiKGyrZ3Hu[EBjcW6maX7nJIRwdWGrbjDpckBpfW2jbjDIeWg4KGOnbHzzJINwNXS{YX7z[oVkfGWmIIfpeIgh\nW\|ZXSgS4FtPC2GQlSgZpkhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR\|UxKD1iMD62OkDPxE1w	NE\UfW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm3N\|I5QCd-MUi5O\|MzQDh:L3G+
CV1	M3rST2Z2dmO2aX;uJIF{e2G7			MlT1RYdwdmm\|dDDhZ5Rqfmm2eTDheEBNYFKjbIDoZUBtcWejbnSgZolv\GmwZzDkc41icW5vbXXkbYF1\WRidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JGNXOSClZXzsd{Bkdy22cnHud4Zm[3SnZDD3bZRpKEejbEStV3JEOSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR\|UxKD1iMD63PVQ{OyEQvF2u	NYHIcpFGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOFUyODJpPkKwN\|Q2OTB{PD;hQi=>
CHOK1	NGnweXpHfW6ldHnvckBie3OjeR?=		NIHk[4szPCCqcoO=	MnfCRYdwdmm\|dDDhZ5Rqfmm2eTDheEBI[Wx2LYTh[4dm\CCOWGLhcJBp[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDDTG9MOSClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOS5\|IN88UU4>	NUHwXHZyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2O\|c3PjRpPkK1Olc4PjZ2PD;hQi=>
HepG2	M1TyNGZ2dmO2aX;uJIF{e2G7			NGXESVlG\m[nY4Sgc44hfHKrZ3z5Z4VzcWSnIHHjZ5VufWyjdHnvckBqdiCqdX3hckBJ\XCJMjDj[YxteyxiRVO1NEA:KDJwMECyJO69VS5?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl5M{K4PEc,OTh7N{OyPFg9N2F-
HepG2	MoLYSpVv[3Srb36gZZN{[Xl?	NFzXdXg2ODBibl2=		NWDWd\|lTUW6qaXLpeIlwdiCxZjCyMUg{NSh\|LTioNk1kcGyxcn:tN{0pfHKrZnz1c5JwdWW2aInsLYJmdnq7bDmoNkwzNWSrcHjlcplt\XSqeXypZY1qdm9rcILvdI95gSmyaHXufYwq[WOndHnjJIFkcWRvaX7keYNm\CC\|cnXidFFkKG2UTlGg[ZhxemW\|c3nvckBqdiCqdX3hckBJ\XCJMjDj[YxteyCjdDC1NFAhdk1?	NFTkSnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEiwNFc3Pyd-MUi4NFA4Pjd:L3G+
HepG2	NHHjUJpHfW6ldHnvckBie3OjeR?=	MWq1NFAhdk1?		M1LXbWlvcGmkaYTpc44hd2ZiMj2oN{0pOy1qKEKtZ4htd3KxLUOtLJRzcW[udX;yc41mfGi7bDni[Y57gWxrKEKsNk1lcXCqZX75cIV1cHmuKXHtbY5wMXC{b4DvfJkqeGinbonsLYFk\XSrYzDhZ4llNWmwZIXj[YQh\mG\|IH3SUmEh\XiycnXzd4lwdiCrbjDoeY1idiCKZYDHNkBk\WyuczDheEA2ODBibl2=	NX\yfIRJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4NFA4PjdpPkG4PFAxPzZ5PD;hQi=>
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Skp2 / pEGFR / EGFR / pERK / ERK ; LXRα / LXRβ / ABCA1 / ABCG1	25184494 11604492
Immunofluorescence	LAMP-1 / LDLR ; pRelA	23382078 26635040
Growth inhibition assay	Cell viability	25184494

In vivo

In mice, GW3965 at a dose of 10 mg/kg upregulates ABCA1 expression 8-fold and raises circulating levels of HDL by 30% with C_max of 12.7 μg/mL and t_1/2 of 2 hours. ^[1] GW3965 (10mg/kg) induces expression of ABCA1 and ABCG1 and shows potent antiatherogenic activity in both LDLR−/− and apoE−/− mice. ^[2] In male sprague-dawley rats, GW3965 reduces Ang II-mediated increases in blood pressure and decreases vascular Ang II receptor gene expression. ^[3] In Glioblastoma mouse model, GW3965 results in inducible degrader of LDLR-mediated LDLR degradation, increased expression of the ABCA1 cholesterol efflux transporter, and thus potently promotes tumor cell death. ^[5]

Protocol (from reference)

Animal Research:^[1]	Animal Models: C57BL/6 mice Dosages: ≤10 mg/kg Administration: Administered via p.o. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	16 mg/mL warmed (25.86 mM)
	Water	Insoluble
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	618.51
Formula	C₃₃H₃₁ClF₃NO₃.HCl
CAS No.	405911-17-3
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC=C(C=C1)C(CN(CCCOC2=CC=CC(=C2)CC(=O)O)CC3=C(C(=CC=C3)C(F)(F)F)Cl)C4=CC=CC=C4.Cl

Download GW3965 HCl SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How to formulate the compound for mouse in vivo experiment?

Answer:
S2630 GW3965 HCl can be dissolved in 2% DMSO/30% PEG 300/dd H2O at 10 mg/mL as a homogeneous suspension. This vehicle is suitable for oral gavage to mice.

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