Molecular Weight(MW): 618.51
GW3965 HCl is a potent, selective LXR agonist for hLXRα and hLXRβ with EC50 of 190 and 30 nM in cell-free assays, respectively.
Cited by 14 Publications
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a. Western blot analysis for EpCAM, PKM2, and CK19 expression in livers from C57BL/6J mice treated with DDC for 1 week in the absence or presence of GW3965 (10 mg/kg; n = 4 per group). *P < 0.05, **P < 0.01. c Western blot analysis of p-STAT3, t-STAT3, and SOCS3 in WB-F344 cells treated with GW3965 (5 μM) or DMSO as control in response to IL-6 (50 ng/ml) treatment for 0, 0.25, 0.5, 0.75, 1, and 2 h.
Arch Toxicol, 2017, 91(1):271-287. GW3965 HCl purchased from Selleck.
GW3965 increased neural progenitor cell (NPC) proliferation concentration-dependently in vitro. (A) The purity of neural progenitor cell (NPC) was determined by immunocytochemistry staining with nestin, a marker of NPC, was labeled in red, and the nucleus was labeled in blue. (B–F) The NPCs were treated with GW3965 (0, 1, 10, 100 μM) for 3 d and subjected to BrdU staining after incorporation for 4 h before fixing, neurosphere initiation medium (NPIM) served as a positive control. (G) Proliferating NPCs showed BrdU positive labeled in green. The statistical values of each group. n=6 wells from three independent experiments. *P < 0.05, **P < 0.01 compared with control, scale bar=100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Exp Neurol, 2018, 304:21-29. GW3965 HCl purchased from Selleck.
Analysis of factor expression in response to LPS + Kavain treatment in cells. To examine whether Kavain affects LITAF translocation via another kinase/factor, a secondary Western Blot was performed. The WT mice macrophages had been untreated or treated with 1 μM GW3965 alone, 5 μM LY294002 alone, or 200 μg/ml Kavain alone as the negative control (lanes 1 or 3–5), or treated with 0.1 μg/ml E. coli LPS alone as the positive control (lane 2), or co‐treated with 0.1 μg/ml LPS + 1 μM GW3965 (lane 6), 0.1 μg/ml LPS + 5 μM LY2940020 (lane 7), or 1 μg/ml LPS + 200 μg/ml Kavain (lane 8) for 1 h. The treated cells’ protein levels or phosphorylation levels were identified by Western Blot, which were then compared to the control (Actin for whole cells protein or Lamin B for nuclear protein). The intensities of each test factor protein (lane 6–8) from LPS alone‐treated cells were assigned to a base value (100%, lane 2). The intensity of the same group from other treatments was calculated relative to this base value after normalization with control (Actin/Lamin B).
J Cell Biochem, 2016, 117(10):2272-80. GW3965 HCl purchased from Selleck.
The effects of GW3965 on NDV infection. (A) The DF-1 cells were cultured with different concentrations of GW3965. Cell viability was determined by MTT assay at 24 h. (B) Synthetic LXR agonist GW3965 inhibited NDV infection in DF-1 cells. Cells were pretreated with GW3965 (1 μM) for 2 h, then infected with NDV (MOI = 1). GW3965 (1 μM) was continuously present during the infection. The expression level of the viral NP protein was determined by western blot. (C) The titers of the supernatant were determined by TCID50. (D) The viral gRNA in cells was detected by qRT-PCR assay.
Arch Virol, 2016, 161(9):2491-501.. GW3965 HCl purchased from Selleck.
Purity & Quality Control
Choose Selective Liver X Receptor Inhibitors
|Description||GW3965 HCl is a potent, selective LXR agonist for hLXRα and hLXRβ with EC50 of 190 and 30 nM in cell-free assays, respectively.|
GW3965 recruits the steroid receptor coactivator 1 to human LXRα with EC50 of 125 nM in a cell-free ligand-sensing assay.  GW3965 shows a potent antagonistic activity against hLXRα and hLXRβ in cell-based assays with EC50 of 190 nM and 30 nM, respectively. Besides, GW3965 also sows excellent selectivity over other nuclear receptors.  In human islets, GW3965 (1 μM) reduces expression of selected pro-inflammatory cytokines including IL-8, monocyte chemotactic protein-1 and tissue factor. 
|In vivo||In mice, GW3965 at a dose of 10 mg/kg upregulates ABCA1 expression 8-fold and raises circulating levels of HDL by 30% with Cmax of 12.7 μg/mL and t1/2 of 2 hours.  GW3965 (10mg/kg) induces expression of ABCA1 and ABCG1 and shows potent antiatherogenic activity in both LDLR−/− and apoE−/− mice.  In male sprague-dawley rats, GW3965 reduces Ang II-mediated increases in blood pressure and decreases vascular Ang II receptor gene expression.  In Glioblastoma mouse model, GW3965 results in inducible degrader of LDLR-mediated LDLR degradation, increased expression of the ABCA1 cholesterol efflux transporter, and thus potently promotes tumor cell death. |
-  Collins JL, et al. J Med Chem. 2002, 45(10), 1963-1966.
-  Joseph SB, et al. Proc Natl Acad Sci U S A. 2002, 99(11), 7604-7609.
-  Leik CE, et al. Br J Pharmacol. 2007, 151(4), 450-456.
|In vitro||DMSO||16 mg/mL warmed (25.86 mM)|
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Frequently Asked Questions
How to formulate the compound for mouse in vivo experiment?
S2630 GW3965 HCl can be dissolved in 2% DMSO/30% PEG 300/dd H2O at 10 mg/mL as a homogeneous suspension. This vehicle is suitable for oral gavage to mice.