Catalog No.S7070

GSK J4 HCl Chemical Structure

Molecular Weight(MW): 453.96

GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.

Size Price Stock Quantity  
In DMSO USD 220 In stock
USD 170 In stock
USD 570 In stock
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2 Customer Reviews

  • DIPG cells were seeded into 96-well plates and treated with panobinostat and GSK-J4 individually or in combination at the indicated concentrations for 72 hr in at least triplicate. Cell viabilities were then assessed using the CelltiterGlo assay relative to 0.1% DMSO control. Data shown as mean ± SD. *indicates the two drugs demonstrate synergy at that condition (i.e. CI < 1).

    Nat Med, 2015, 21(6):555-9. . GSK J4 HCl purchased from Selleck.

    GSK-J4 impaired hair cell regeneration after neomycin damage. (A–D) GSK-J4 reduced the numbers of GFP-positive (green) and FM1-43FX-positive (red) hair cells compared with DMSO-treated controls. Scale bars = 10 μm. (E,F) Quantitative analysis of the number of GFP-positive (E) or FM1-43FX-positive (F) hair cells per neuromast (NM) at different time points in DMSO-treated control and GSK-J4-treated larvae. In the 24-h group, n = 40 neuromasts (20 larvae) per group; in the 48-h group, n = 28 neuromasts (14 larvae) per group. ***p < 0.0001. Bars are mean ± sem. [24-h group: One-way ANOVA; GFP-positive cells: F(2, 117) = 96.94; FM1-43FX-positive cells: F(2, 117) = 114. 48-h group: One-way ANOVA; GFP-positive cells: F(2, 81) = 88.96; FM1-43FX-positive cells: F(2, 81) = 93.85].

    Front Mol Neurosci, 2017, doi: 10.3389/fnmol.2017.00051. GSK J4 HCl purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.
JMJD3 [1]
(Cell-free assay)
60 nM
In vitro

GSK J4 HCl is an ethyl ester derivative of the JMJD3 selective histone demethylase inhibitor GSK-J1 with an IC50 value greater than 50 μM in vitro. GSK J4 HCl is used to probe the consequences of demethylation of H3K27me3. In human primary macrophages, GSK-J4 inhibits the lipopolysaccharide-induced production of cytokines, including pro-inflammatory tumour necrosis factor (TNF). In addition, GSK-J4 prevents the lipopolysaccharide-induced loss of H3K27me3 associated with the TNF transcription start sites and blocked the recruitment of RNA polymerase II. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CUTLL1 NEOzU3VIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M1;ESlIh|ryP MlnaSG1UVw>? MlToZYZn\WO2czDj[YxtKGe{b4f0bC=> MnnvNlUyOzJ3NEm=
CUTLL1 NI\WfZJCeG:ydH;zbZMh[XO|YYm= MlLXNkDPxE1? M4qye2ROW09? MoPmbY5lfWOnczDhdI9xfG:|aYO= MoTwNlUyOzJ3NEm=
CUTLL1 MYfGeY5kfGmxbjDhd5NigQ>? NX;XdnA2OiEQvF2= NU\UfoU4TE2VTx?= MoTGbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dB?= MlLhNlUyOzJ3NEm=
CUTLL1 MojvT4lv[XOnIHHzd4F6 NWrKWnp5PiEQvF2= M3X0U2ROW09? M4XPeoxm[WS|IITvJIlv[3KnYYPl[EBJO0t{N33lNy=> MXqyOVE{OjV2OR?=
SF7761 MknFT4lv[XOnIHHzd4F6 M{XxNVYh|ryP NV;j[I5GTE2VTx?= NI\OTGxqdmO{ZXHz[ZMhUzJ5IH3leIh6dGG2aX;u MYqyOVQxOTZ7Mx?=
SF8628 M4rX[2tqdmG|ZTDhd5NigQ>? NIfBT4U3KM7:TR?= NE\QUHdFVVOR MVfpcoNz\WG|ZYOgT|I5KG2ndHj5cIF1cW:w NF34dWQzPTRyMU[5Ny=>
H3.3 NV;vOXZ[U2mwYYPlJIF{e2G7 NXn5VpE5PiEQvF2= MUPEUXNQ M{i5O4lv[3KnYYPld{BMOjlibXX0bJlt[XSrb36= MYCyOVQxOTZ7Mx?=
SF9012 NIjCSZdMcW6jc3WgZZN{[Xl? NUHGTZBpPiEQvF2= NHnWR3dFVVOR MoS4bY5kemWjc3XzJGs{OCCvZYTofYxifGmxbh?= MWGyOVQxOTZ7Mx?=
SF9402 NHXZTY5McW6jc3WgZZN{[Xl? MVu2JO69VQ>? M2fDe2ROW09? NXS0SWF[cW6lcnXhd4V{KEt|MTDt[ZRpgWyjdHnvci=> NWS0UWltOjV2MEG2PVM>
SF9427 MX\LbY5ie2ViYYPzZZk> MkK5OkDPxE1? M1vPN2ROW09? M1nHeIlv[3KnYYPld{BMOzJibXX0bJlt[XSrb36= Mn\xNlU1ODF4OUO=
human astrocytes NHfH[GtMcW6jc3WgZZN{[Xl? M{SyTFYh|ryP NYflSHNDTE2VTx?= MWjpcoNz\WG|ZYOgT|M{KG2ndHj5cIF1cW:w M2PEVlI2PDBzNkmz
SF7761 NXrwcY9CT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MX62JO69VQ>? MmHiSG1UVw>? NWHDeXE4cW6qaXLpeJMhUzJ5TTDncIlwdWFiY3XscEBoem:5dHi= NEHp[I8zPTRyMU[5Ny=>
SF8628 MnvkS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NF;yN5I3KM7:TR?= MkjMSG1UVw>? NV3zTJhqcW6qaXLpeJMhUzJ6TTDncIlwdWFiY3XscEBoem:5dHi= M3jBZ|I2PDBzNkmz
H3.3 M2PMPGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NYHOSlRPPiEQvF2= MULEUXNQ NU[2ZYFycW6qaXLpeJMhUzJ7TTDncIlwdWFiY3XscEBoem:5dHi= MUmyOVQxOTZ7Mx?=
SF9012 NILVd4ZIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NF\S[VA3KM7:TR?= MWTEUXNQ MV7pcohq[mm2czDLN|BOKGeuaX;tZUBk\WyuIHfyc5d1cA>? MYCyOVQxOTZ7Mx?=
SF9402 NYjZb4xPT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= M3XU[VYh|ryP NV7nPIRvTE2VTx?= MnTXbY5pcWKrdIOgT|MyVSCpbHnvcYEh[2WubDDndo94fGh? NFezXoUzPTRyMU[5Ny=>
SF9427 NFjafW9Iem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MnO4OkDPxE1? M2rKXGROW09? NW\XeWh4cW6qaXLpeJMhUzN{TTDncIlwdWFiY3XscEBoem:5dHi= MnHoNlU1ODF4OUO=
human astrocytes NUD1Xnh[T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MYW2JO69VQ>? M3i1cWROW09? NVLkSo5FcW6qaXLpeJMhUzN|TTDncIlwdWFiY3XscEBoem:5dHi= NYTKe2FuOjV2MEG2PVM>
TG neurons NIPz[4lHfW6ldHnvckBie3OjeR?= M1:zSFUxKM7:TR?= NISzWVNFVVOR MmrYbY5pcWKrdIOgTHNXNTFicnXhZ5RqfmG2aX;uJIZzd21ic3Xud49zgSCwZYXyc45{ NWXrS3h2OjV3NUK3NlA>
Th17 M1zFN2Z2dmO2aX;uJIF{e2G7 Mnz4PFAhdk1? NF7pNllFVVOR M2T0bIlvcGmkaYTzJINmdGxiZHnm[oVz\W62aXH0bY9v MYWyOVg1ODl7Mx?=
β-cells NITpZmdHfW6ldHnvckBie3OjeR?= NIqzWoEzOCEQvF2= NXnnfFJnTE2VTx?= MUnicJVvfHNiSV\O{tMtKEmuLUJOtkwh[W6mIGTOSu6yNWmwZIXj[YQh[2inbX;rbY5mKGenbnWg[ZhxemW|c3nvci=> NGnuR2MzPjVyNUG5Ny=>
β-cells NGfPclZHfW6ldHnvckBie3OjeR?= MYCyNEDPxE1? NUDjPYRoTE2VTx?= NES5PWdqdmS3Y3XzJO6zNWOnbHyg[Jl{\nWwY4Tpc44> M3fmVFI3PTB3MUmz
ESCs M3\YTmZ2dmO2aX;uJIF{e2G7 NVL1bHVEOS564pEFxtVO NIXPR3JFVVOR MlTObY5lfWOnczDEUmEh\GGvYXflJIFtd26pIIfpeIgh[WO2aY\heIlwdiCxZjD0bIUhTE6DIHThcYFo\SC{ZYPwc45{\Q>? NEn5TZIzPjd3OUG3OS=>
Raw 264.7 MmTXSpVv[3Srb36gZZN{[Xl? MU[wMlgyQTMkgJZCuW0> M3XzZmROW09? NUHOSlJvcW6qaXLpeJMhXE6ILd8xJJBzd2S3Y4Tpc44> NI\wNGozPjd5NkO2NC=>

... Click to View More Cell Line Experimental Data


Solubility (25°C)

In vitro DMSO 90 mg/mL warmed (198.25 mM)
Water 90 mg/mL (198.25 mM)
Ethanol 90 mg/mL warmed (198.25 mM)
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+dd H2O
For best results, use promptly after mixing.
10 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 453.96


CAS No. 1373423-53-0(free base)
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I cannot get a clear solution when I dissolve the compound into water.

  • Answer:

    S7070 GSK J4 HCl can be dissolved in 2% DMSO/dd H2O at 10 mg/mL as a clear solution. Water cannot dissolve it clear directly.

Histone Demethylase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID