GSK J4 HCl (GSKJ4 HCl)

For research use only.

Catalog No.S7070

32 publications

GSK J4 HCl (GSKJ4 HCl) Chemical Structure

CAS No. 1797983-09-5

GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.

Size Price Stock Quantity  
10mM (1mL in DMSO) EUR 216 In stock
EUR 167 In stock
EUR 560 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's GSK J4 HCl (GSKJ4 HCl) has been cited by 32 publications

Purity & Quality Control

Choose Selective Histone Demethylase Inhibitors

Biological Activity

Description GSK J4 HCl is a cell permeable prodrug of GSK J1, which is the first selective inhibitor of the H3K27 histone demethylase JMJD3 and UTX with IC50 of 60 nM in a cell-free assay and inactive against a panel of demethylases of the JMJ family.
Targets
JMJD3 [1]
(Cell-free assay)
60 nM
In vitro

GSK J4 HCl is an ethyl ester derivative of the JMJD3 selective histone demethylase inhibitor GSK-J1 with an IC50 value greater than 50 μM in vitro. GSK J4 HCl is used to probe the consequences of demethylation of H3K27me3. In human primary macrophages, GSK-J4 inhibits the lipopolysaccharide-induced production of cytokines, including pro-inflammatory tumour necrosis factor (TNF). In addition, GSK-J4 prevents the lipopolysaccharide-induced loss of H3K27me3 associated with the TNF transcription start sites and blocked the recruitment of RNA polymerase II. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CUTLL1 M1u1bmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NFjYW5IzKM7:TR?= MmfkSG1UVw>? M4Dwe4Fn\mWldIOgZ4VtdCCpcn;3eIg> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF|MkW0PUc,OjVzM{K1OFk9N2F-
CUTLL1 M4O0VWFxd3C2b4Ppd{Bie3OjeR?= MknYNkDPxE1? NFHsNnBFVVOR M{ezfIlv\HWlZYOgZZBweHSxc3nz M1XCXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUOyOVQ6Lz5{NUGzNlU1QTxxYU6=
CUTLL1 NFz1RZJHfW6ldHnvckBie3OjeR?= M3LyN|Ih|ryP MX7EUXNQ NGO0cWdqdmS3Y3XzJINmdGxiY4njcIUh[XK{ZYP0 MmHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzM{K1OFkoRjJ3MUOyOVQ6RC:jPh?=
CUTLL1 NHTJZo5McW6jc3WgZZN{[Xl? NV;aZmVIPiEQvF2= NVmwWlRDTE2VTx?= NEXTSnBt\WGmczD0c{BqdmO{ZXHz[YQhUDONMkft[VM> NHfyUZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGzNlU1QSd-MkWxN|I2PDl:L3G+
SF7761 NGK2bWhMcW6jc3WgZZN{[Xl? NU\RbpFoPiEQvF2= MnSzSG1UVw>? NXPYcZNncW6lcnXhd4V{KEt{NzDt[ZRpgWyjdHnvci=> M3HsfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
SF8628 NUPVc5U4U2mwYYPlJIF{e2G7 MV62JO69VQ>? MYrEUXNQ MW\pcoNz\WG|ZYOgT|I5KG2ndHj5cIF1cW:w M{f0dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
H3.3 NFPQd2pMcW6jc3WgZZN{[Xl? MnzsOkDPxE1? MlS5SG1UVw>? MWTpcoNz\WG|ZYOgT|I6KG2ndHj5cIF1cW:w M3HoOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
SF9012 M{ewOmtqdmG|ZTDhd5NigQ>? M{[xe|Yh|ryP MVfEUXNQ M3zYc4lv[3KnYYPld{BMOzBibXX0bJlt[XSrb36= Ml\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MEG2PVMoRjJ3NECxOlk{RC:jPh?=
SF9402 NHfqfIpMcW6jc3WgZZN{[Xl? MWm2JO69VQ>? M3ntdGROW09? MXHpcoNz\WG|ZYOgT|MyKG2ndHj5cIF1cW:w NITBdmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwNVY6Oyd-MkW0NFE3QTN:L3G+
SF9427 Ml:1T4lv[XOnIHHzd4F6 MlHSOkDPxE1? NI\SdJdFVVOR NIq0[G1qdmO{ZXHz[ZMhUzN{IH3leIh6dGG2aX;u M3;ONVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
human astrocytes M3S1cmtqdmG|ZTDhd5NigQ>? MlTQOkDPxE1? NVTkUY04TE2VTx?= NFPpZ2xqdmO{ZXHz[ZMhUzN|IH3leIh6dGG2aX;u M{\aVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
SF7761 NH7se2RIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MlfDOkDPxE1? NVi4O40yTE2VTx?= M3jLVYlvcGmkaYTzJGszP01iZ3zpc41iKGOnbHyg[5Jwf3Sq MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTRyMU[5N{c,OjV2MEG2PVM9N2F-
SF8628 NGW5W4xIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M33oOlYh|ryP NF7PeFVFVVOR MYPpcohq[mm2czDLNlhOKGeuaX;tZUBk\WyuIHfyc5d1cA>? M17aPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NECxOlk{Lz5{NUSwNVY6OzxxYU6=
H3.3 M1;lTWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 Mn7FOkDPxE1? Mmq0SG1UVw>? MWnpcohq[mm2czDLNllOKGeuaX;tZUBk\WyuIHfyc5d1cA>? NFTBcoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwNVY6Oyd-MkW0NFE3QTN:L3G+
SF9012 NUfYVplOT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NVLjPJVHPiEQvF2= NGi1dpRFVVOR NVrIR3JGcW6qaXLpeJMhUzNyTTDncIlwdWFiY3XscEBoem:5dHi= NEjwPIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwNVY6Oyd-MkW0NFE3QTN:L3G+
SF9402 NHvtPHBIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MmPCOkDPxE1? MWXEUXNQ NFvXdnZqdmirYnn0d{BMOzGPIHfsbY9u[SClZXzsJIdzd3e2aB?= NHnJNXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSwNVY6Oyd-MkW0NFE3QTN:L3G+
SF9427 MoTMS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NVvZW5RFPiEQvF2= MVTEUXNQ NYDoXWljcW6qaXLpeJMhUzN{TTDncIlwdWFiY3XscEBoem:5dHi= MlfiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MEG2PVMoRjJ3NECxOlk{RC:jPh?=
human astrocytes MWfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NWPzZlJHPiEQvF2= NYnzV2F4TE2VTx?= MoLSbY5pcWKrdIOgT|M{VSCpbHnvcYEh[2WubDDndo94fGh? NWftXlRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0NFE3QTNpPkK1OFAyPjl|PD;hQi=>
TG neurons M3vOOmZ2dmO2aX;uJIF{e2G7 NX7U[o1[PTBizszN NI\GNlZFVVOR NYCyUYtKcW6qaXLpeJMhUFOYLUGgdoVi[3SrdnH0bY9vKG[{b32gd4Vve2:{eTDu[ZVzd26| NWrXS2dlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVI4OjBpPkK1OVUzPzJyPD;hQi=>
Th17 NHjhZppHfW6ldHnvckBie3OjeR?= NYO3OldpQDBibl2= NHnUfW1FVVOR NYrWcZJxcW6qaXLpeJMh[2WubDDkbYZn\XKnboTpZZRqd25? NF;KVm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi0NFk6Oyd-MkW4OFA6QTN:L3G+
β-cells MXHGeY5kfGmxbjDhd5NigQ>? NXqweIhlOjBizszN M2rIUGROW09? MUTicJVvfHNiSV\O{tMtKEmuLUJOtkwh[W6mIGTOSu6yNWmwZIXj[YQh[2inbX;rbY5mKGenbnWg[ZhxemW|c3nvci=> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjVyNUG5N{c,OjZ3MEWxPVM9N2F-
β-cells M4jIUWZ2dmO2aX;uJIF{e2G7 MlHFNlAh|ryP MnzHSG1UVw>? M3jlOolv\HWlZYOg{tIu[2WubDDkfZNnfW6ldHnvci=> NX:yRohFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1NFUyQTNpPkK2OVA2OTl|PD;hQi=>
ESCs M3noNGZ2dmO2aX;uJIF{e2G7 M{G5PVEvQOLChdM1US=> NWPZR5lPTE2VTx?= MV\pcoR2[2W|IFTORUBl[W2jZ3WgZYxwdmdid3n0bEBi[3SrdnH0bY9vKG:oIITo[UBFVkFiZHHtZYdmKHKnc4DvcpNm MnLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5NUmxO|UoRjJ4N{W5NVc2RC:jPh?=
Raw 264.7 MVnGeY5kfGmxbjDhd5NigQ>? MYmwMlgyQTMkgJZCuW0> MlHMSG1UVw>? M33kcYlvcGmkaYTzJHRPTi4QsTDwdo9lfWO2aX;u M4e4U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{e2N|YxLz5{Nke3OlM3ODxxYU6=
MCF7 MVTGeY5kfGmxbjDhd5NigQ>? MnvZNUB1dyBzMDD1US=> NUW4[2pCOzBiaILz MnPXTY5pcWKrdHnvckBw\iCNRF21RUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHXm[oVkfCCxbjDIN2s1dWV|IH3leIh6dGG2aX;uJIxmfmWuczDheEAyKHSxIEGwJJVOKGGodHXyJFMxKGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NHXZbXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO5NlM1QSd-M{CzPVI{PDl:L3G+
MCF7 NYCxSZNVTnWwY4Tpc44h[XO|YYm= NV36TIg{OSC2bzCxNEB2VQ>? M{jWVlMxKGi{cx?= MojKTY5pcWKrdHnvckBw\iCNRF21RUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHXm[oVkfCCxbjDIN2szP22nMzDt[ZRpgWyjdHnvckBt\X[nbIOgZZQhOSC2bzCxNEB2VSCjZoTldkA{OCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NX7FTHhbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzPVI{PDlpPkOwN|kzOzR7PD;hQi=>
RAW264.7 MXmwMlgzKHWP NUnue2JFOjRiaILz NYT4fJh4UW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBVVk[jbIDoZUBxem:mdXP0bY9vKGmwIH3veZNmKFKDV{K2OE44KGOnbHzzJFAvQDJidV2gZYZ1\XJiMkSgbJJ{KGK7IFXMTXNC NWTaVlhPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3O|Y{PjBpPkK2O|c3OzZyPD;hQi=>
RAW264.7 M3\FOVI1KGi{cx?= NIDJTYZKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJHRPTmGucHjhJJBzd2S3Y4Tpc44hcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNC NGHqbY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke3OlM3OCd-Mk[3O|Y{PjB:L3G+

... Click to View More Cell Line Experimental Data

Protocol

Solubility (25°C)

In vitro DMSO 90 mg/mL warmed (198.25 mM)
Water 90 mg/mL (198.25 mM)
Ethanol ''90 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 453.96
Formula

C24H27N5O2.HCl

CAS No. 1797983-09-5
Storage powder
in solvent
Synonyms N/A
Smiles CCOC(=O)CCNC1=CC(=NC(=N1)C2=CC=CC=N2)N3CCC4=CC=CC=C4CC3.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I cannot get a clear solution when I dissolve the compound into water.

  • Answer:

    S7070 GSK J4 HCl can be dissolved in 2% DMSO/dd H2O at 10 mg/mL as a clear solution. Water cannot dissolve it clear directly.

Histone Demethylase Signaling Pathway Map

Tags: buy GSK J4 HCl (GSKJ4 HCl) | GSK J4 HCl (GSKJ4 HCl) supplier | purchase GSK J4 HCl (GSKJ4 HCl) | GSK J4 HCl (GSKJ4 HCl) cost | GSK J4 HCl (GSKJ4 HCl) manufacturer | order GSK J4 HCl (GSKJ4 HCl) | GSK J4 HCl (GSKJ4 HCl) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID