GSK-LSD1 2HCl

Catalog No.S7574

For research use only.

GSK-LSD1 2HCl is an irreversible, and selective LSD1 inhibitor with IC50 of 16 nM, > 1000 fold selective over other closely related FAD utilizing enzymes (i.e. LSD2, MAO-A, MAO-B).

GSK-LSD1 2HCl Chemical Structure

CAS No. 2102933-95-7

Selleck's GSK-LSD1 2HCl has been cited by 16 publications

Purity & Quality Control

Choose Selective Histone Demethylase Inhibitors

Biological Activity

Description GSK-LSD1 2HCl is an irreversible, and selective LSD1 inhibitor with IC50 of 16 nM, > 1000 fold selective over other closely related FAD utilizing enzymes (i.e. LSD2, MAO-A, MAO-B).
Targets
LSD1 [1]
(Cell-free assay)
16 nM
In vitro

GSK-LSD1 induces gene expression changes in cancer cell lines with average EC50 of < 5 nM and inhibits cancer cell line growth with average EC50 of < 5 nM. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 M2i4VGZ2dmO2aX;uJIF{e2G7 MofkNVAhfU1? Mnf5NUBpeg>? NWTEOnl1SmmwZHnu[{Bi\m[rbnn0fUB1dyCOU1SxJIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd5Ri[mmuaYT5JI9nKGOnbHz1cIFzKEyVREGgdJJwfGWrbjDheEAyOCC3TTDwdoUucW6ldXLheIVlKG[xcjCxJIhzKG[xbHzve4VlKGK7IHjlZZRqdmdiYYSgOFkh\GWpQzDmc5IhOyCvaX7zJIJ6KFenc4Tldo4h[myxdD3iZZNm\CCFRWPURS=> M1LWPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyN{iwNFg4Lz5|MEe4NFA5PzxxYU6=
A549 M3v2U2Z2dmO2aX;uJIF{e2G7 NWjNXlZrOTBidV2= MmDYNUBpeg>? MWHCbY5lcW6pIHHm[olvcXS7IITvJGxUTDFiaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiC|dHHibYxqfHlib3[gZ4VtdHWuYYKgUHNFOSCycn;0[YlvKGG2IEGwJJVOKHC{ZT3pcoN2[mG2ZXSg[o9zKDFiaIKg[o9tdG:5ZXSgZpkhcGWjdHnu[{BifCB3MjDk[YdEKG[xcjCzJI1qdnNiYomgW4V{fGW{bjDicI91NWKjc3XkJGNGW1SD NXPRdY1ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PFAxQDdpPkOwO|gxODh5PD;hQi=>
A549 NYLtNHRDTnWwY4Tpc44h[XO|YYm= NF3yUGg6PiCqcoO= MnjPSI94diC{ZXf1cIF1cW:wIH;mJJNv[WmuIHzleoVteyCrbjDoeY1idiCDNUS5JINmdGy|IIXwJJRwKDF4IIXNJIFnfGW{IEm2JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M3KybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyN{iwNFg4Lz5|MEe4NFA5PzxxYU6=
A549 NHWzO5RHfW6ldHnvckBie3OjeR?= NFzYbHk6PiCqcoO= NHPUSllFd3ewIILl[5Vt[XSrb36gc4YhVi2lYXTo[ZJqdiCuZY\lcJMhcW5iaIXtZY4hSTV2OTDj[YxteyC3cDD0c{AyPiC3TTDh[pRmeiB7NjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MmCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB5OECwPFcoRjNyN{iwNFg4RC:jPh?=
A549 M1;JVGZ2dmO2aX;uJIF{e2G7 MmX5PVYhcHK| MVHEc5dvKHKnZ4XsZZRqd25ib3[gd4x2\yCuZY\lcJMhcW5iaIXtZY4hSTV2OTDj[YxteyC3cDD0c{AyPiC3TTDh[pRmeiB7NjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NXrwZ21XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PFAxQDdpPkOwO|gxODh5PD;hQi=>
A549 NF25RYpHfW6ldHnvckBie3OjeR?= NGLTRXg6PiCqcoO= NF:0eVRWeCC{ZXf1cIF1cW:wIH;mJINt[XWmaX6tNUBt\X[nbIOgbY4hcHWvYX6gRVU1QSClZXzsd{B2eCC2bzCxOkB2VSCjZoTldkA6PiCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NEf6e5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEe4NFA5Pyd-M{C3PFAxQDd:L3G+
A549 NE\jNFVHfW6ldHnvckBie3OjeR?= NFP1Z3M6PiCqcoO= NEHQNGlWeCC{ZXf1cIF1cW:wIH;mJGUu[2GmaHXybY4hdGW4ZXzzJIlvKGi3bXHuJGE2PDliY3XscJMhfXBidH:gNVYhfU1iYX\0[ZIhQTZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NY[0UFM4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PFAxQDdpPkOwO|gxODh5PD;hQi=>
THP1 MW\GeY5kfGmxbjDhd5NigQ>? MmX6OEB2VQ>? MYKzJIRigXN? M2[xdGlvcGmkaYTpc44hd2ZiTGPENUBqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGNFQDZiZYjwdoV{e2mxbjDheEA1KHWPIHnuZ5Vj[XSnZDDmc5IhOyCmYYnzJIJ6KG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> MnjIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyOU[xOVYoRjNzMEm2NVU3RC:jPh?=
Assay
Methods Test Index PMID
Western blot LSD1 / H3K4me2 29088714

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 289.24
Formula

C14H22Cl2N2

CAS No. 2102933-95-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1CNCCC1NC2CC2C3=CC=CC=C3.Cl.Cl

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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