Durvalumab (anti-PD-L1)

Catalog No.A2013 Synonyms: MEDI4736

For research use only.
Not for use in humans.

Durvalumab (anti-PD-L1) is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.

Durvalumab (anti-PD-L1)

Selleck's Durvalumab (anti-PD-L1) has been cited by 9 Publications

Purity & Quality Control

Choose Selective PD-1/PD-L1 Inhibitors

Biological Activity

Description Durvalumab (anti-PD-L1) is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.
Targets
PD-L1/CD80 [1]
(HTRF assay)
PD-1/PD-L1 interaction [1]
(HTRF assay)
0.04 nM 0.1 nM
In vitro

The labeled radioligand shows good affinity to high PD-L1 expression cells and could be blocked with excess unlabeled intact durvalumab.[1]

In vivo

The peak tumor uptake of 124I-Durva-F(ab’)2 was close to 124I-Durva, but much earlier (5.29±0.42% ID/g for 124I-Durva-F(ab’)2 at 12 h vs 5.18±0.73% ID/g for 124I-Durva at 48 h). Compared with 124I-Durva, an accelerated blood clearance was observed for 124I-Durva-F(ab’)2, allowing for a higher tumor-to-background ratio.[1]

Protocol (from reference)

Cell Research:
  • The A549 and H460 cells were cultured in six-well plates (~106 cells/well) and divided into two groups. All of the wells were incubated with 5% BSA for blocking nonspecific binding. Group A was presaturated with 100-fold excess of unlabeled durvalumab to block the binding of 124I-Durva-F(ab′)2, and group B had only the medium added. After 30 min, 2 nM of 124I-Durva-F(ab′)2 was added to each group and incubated for 1 h at 37 °C in a cell incubator. After supernatant collection, the cells were lysed using 2 M of NaOH.

Animal Research:
  • Animal Models: H460 and A549 tumor-bearing mice
    Dosages: 20 μCi
    Administration: i.v.
    Reference: https://pubmed.ncbi.nlm.nih.gov/35244407/
     

Product Details

CAS No. 1428935-60-7
Formulation PBS buffer, pH 7.2
Isotype Human IgG1
Source CHO cells
Storage Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

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