Name: DMOG (Dimethyloxallyl Glycine)
Brand: Selleck Chemicals
SKU: S7483
Availability: InStock
Rating: 5 (52 reviews)

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Quality Control

Batch: Purity: 99.92%

99.92

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Hydroxylase Inhibitors	Ro 61-8048 Meldonium 4-Chloro-DL-phenylalanine (R)-Nepicastat HCl Nepicastat (SYN-117) HCl H-Tyr(3-I)-OH 2-Thiouracil

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Activity Description	PMID
human SR cell	Growth inhibition assay		Inhibition of human SR cell growth in a cell viability assay, IC50=4.57946 μM	SANGER
human LB996-RCC cell	Growth inhibition assay		Inhibition of human LB996-RCC cell growth in a cell viability assay, IC50=5.6877 μM	SANGER
human LOUCY cell	Growth inhibition assay		Inhibition of human LOUCY cell growth in a cell viability assay, IC50=10.3493 μM	SANGER
human KS-1 cell	Growth inhibition assay		Inhibition of human KS-1 cell growth in a cell viability assay, IC50=11.2557 μM	SANGER
human U-698-M cell	Growth inhibition assay		Inhibition of human U-698-M cell growth in a cell viability assay, IC50=13.3306 μM	SANGER
human A3-KAW cell	Growth inhibition assay		Inhibition of human A3-KAW cell growth in a cell viability assay, IC50=15.8174 μM	SANGER
human BL-70 cell	Growth inhibition assay		Inhibition of human BL-70 cell growth in a cell viability assay, IC50=15.9423 μM	SANGER
human DMS-273 cell	Growth inhibition assay		Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=15.965 μM	SANGER
human CAL-39 cell	Growth inhibition assay		Inhibition of human CAL-39 cell growth in a cell viability assay, IC50=19.3277 μM	SANGER
human KYSE-410 cell	Growth inhibition assay		Inhibition of human KYSE-410 cell growth in a cell viability assay, IC50=20.8197 μM	SANGER
human YT cell	Growth inhibition assay		Inhibition of human YT cell growth in a cell viability assay, IC50=23.3984 μM	SANGER
human LC-2-ad cell	Growth inhibition assay		Inhibition of human LC-2-ad cell growth in a cell viability assay, IC50=24.5573 μM	SANGER
human COR-L105 cell	Growth inhibition assay		Inhibition of human COR-L105 cell growth in a cell viability assay, IC50=26.4436 μM	SANGER
human CAL-12T cell	Growth inhibition assay		Inhibition of human CAL-12T cell growth in a cell viability assay, IC50=26.5502 μM	SANGER
human KU812 cell	Growth inhibition assay		Inhibition of human KU812 cell growth in a cell viability assay, IC50=28.4579 μM	SANGER
human OPM-2 cell	Growth inhibition assay		Inhibition of human OPM-2 cell growth in a cell viability assay, IC50=28.8227 μM	SANGER
human SW982 cell	Growth inhibition assay		Inhibition of human SW982 cell growth in a cell viability assay, IC50=33.7007 μM	SANGER
human HH cell	Growth inhibition assay		Inhibition of human HH cell growth in a cell viability assay, IC50=34.0235 μM	SANGER
human RS4-11 cell	Growth inhibition assay		Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=34.1806 μM	SANGER
human BEN cell	Growth inhibition assay		Inhibition of human BEN cell growth in a cell viability assay, IC50=36.8187 μM	SANGER
human CCRF-CEM cell	Growth inhibition assay		Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=37.7729 μM	SANGER
human EW-13 cell	Growth inhibition assay		Inhibition of human EW-13 cell growth in a cell viability assay, IC50=39.0481 μM	SANGER
human NCI-H2228 cell	Growth inhibition assay		Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=42.818 μM	SANGER
human KE-37 cell	Growth inhibition assay		Inhibition of human KE-37 cell growth in a cell viability assay, IC50=43.2915 μM	SANGER
human A4-Fuk cell	Growth inhibition assay		Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=45.0364 μM	SANGER
human MZ1-PC cell	Growth inhibition assay		Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50=45.9888 μM	SANGER
human MLMA cell	Growth inhibition assay		Inhibition of human MLMA cell growth in a cell viability assay, IC50=46.3292 μM	SANGER
human EB-3 cell	Growth inhibition assay		Inhibition of human EB-3 cell growth in a cell viability assay, IC50=49.5792 μM	SANGER
human 293T cells	Function assay	2.5 mM	Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM	19359167
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 35 mg/mL (199.84 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 35 mg/mL

Ethanol : 35 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.750mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 35 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	175.14	Formula	C₆H₉NO₅	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	89464-63-1	Download SDF		Storage of Stock Solutions	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms	Dimethyloxalylglycine			Smiles	COC(=O)CNC(=O)C(=O)OC

Mechanism of Action

Targets/IC₅₀/Ki	HIF prolyl hydroxylase
In vitro	DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. This compound reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.
In vivo	DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by this compound enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/8002959/ [2] https://pubmed.ncbi.nlm.nih.gov/15319416/ [3] https://pubmed.ncbi.nlm.nih.gov/19304911/ [4] https://pubmed.ncbi.nlm.nih.gov/24389644/

Applications

Methods	Biomarkers	Images	PMID
Western blot	HIF-1α p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM WNT11 / HIF-2α		29555474
Immunofluorescence	HIF1α		28955984

Tech Support

Handling Instructions

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DMOG (Dimethyloxallyl Glycine) HIF-PH Inhibitor

Chemical Structure

Molecular Weight: 175.14