DMOG

Catalog No.S7483 Synonyms: Dimethyloxalylglycine

DMOG Chemical Structure

Molecular Weight(MW): 175.14

DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus.

Size Price Stock Quantity  
In DMSO USD 120 In stock
USD 97 In stock
USD 194 In stock
USD 327 In stock
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Cited by 6 Publications

5 Customer Reviews

  • Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

    Hepatology, 2018, 68(6):2359-2375. DMOG purchased from Selleck.

    Western blot analysis of the indicated proteins after the treatment with DMOG (K).

    Theranostics, 2017, 7(13): 3293-3305. DMOG purchased from Selleck.

  • Western blot analysis of the indicated proteins after the treatment with DMOG.

    Theranostics, 2017, 7(13):3293-3305. DMOG purchased from Selleck.

    OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

    Clin Exp Immunol, 2018, doi:10.1111/cei.13227. DMOG purchased from Selleck.

  • (A) Representative western blot images for appoptosin and HIF-1α protein expression in control, DMOG and 1,4-DPCA groups. (B) Protein expression levels of appoptosin and HIF-1α in control, DMOG and 1,4-DPCA groups were quantified by densitometric analysis.

    Mol Med Rep, 2018, 17(5):7149-7155. DMOG purchased from Selleck.

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Biological Activity

Description DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus.
Targets
HIF prolyl hydroxylase [1]
()
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell NULjSIY5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXHJcohq[mm2aX;uJI9nKGi3bXHuJHNTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC53N{m0OkDPxE1? MUfTRW5ITVJ?
human LB996-RCC cell M{G4Omdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUmxfG5xUW6qaXLpeIlwdiCxZjDoeY1idiCOQkm5Ok1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lk[4O|ch|ryP NFf3fVZUSU6JRWK=
human LOUCY cell NVnROpZJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoK2TY5pcWKrdHnvckBw\iCqdX3hckBNV1WFWTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlM1QTNizszN NX;tNZF5W0GQR1XS
human KS-1 cell NEDyfZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlvlTY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlU2PyEQvF2= NIXzNWZUSU6JRWK=
human U-698-M cell NYLVWo84T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV3Jcohq[mm2aX;uJI9nKGi3bXHuJHUuPjl6LV2gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4{OzB4IN88US=> NEDkSoRUSU6JRWK=
human A3-KAW cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2TLeWlvcGmkaYTpc44hd2ZiaIXtZY4hSTNvS1HXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQDF5NDFOwG0> NVG4XWR{W0GQR1XS
human BL-70 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlPGTY5pcWKrdHnvckBw\iCqdX3hckBDVC15MDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG1Mlk1OjNizszN MWrTRW5ITVJ?
human DMS-273 cell Mkf4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkWwTY5pcWKrdHnvckBw\iCqdX3hckBFVVNvMkezJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQTZ3IN88US=> MoD4V2FPT0WU
human CAL-39 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG5MlMzPzdizszN M4C3TXNCVkeHUh?=
human KYSE-410 cell Moq1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2\KU2lvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOC56MUm3JO69VQ>? NVHrTWFDW0GQR1XS
human YT cell NFnPUoFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHPPXlNKdmirYnn0bY9vKG:oIHj1cYFvKFmWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuN|k5PCEQvF2= M1W1NnNCVkeHUh?=
human LC-2-ad cell Mmi3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHJcohq[mm2aX;uJI9nKGi3bXHuJGxENTJvYXSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE42PTd|IN88US=> NIfHc4NUSU6JRWK=
human COR-L105 cell M2rDb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX;Jcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlYvPDR|NjFOwG0> M3HhVHNCVkeHUh?=
human CAL-12T cell M3\Dcmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mk\RTY5pcWKrdHnvckBw\iCqdX3hckBESUxvMULUJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlYvPTVyMjFOwG0> NXO2XHJPW0GQR1XS
human KU812 cell MlnrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYrJcohq[mm2aX;uJI9nKGi3bXHuJGtWQDF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuOFU4QSEQvF2= M2HhenNCVkeHUh?=
human OPM-2 cell MmLKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJG9RVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuPFIzPyEQvF2= Mm\NV2FPT0WU
human SW982 cell M4PEeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVzJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{OuO|AxPyEQvF2= M3\pPHNCVkeHUh?=
human HH cell NV;3b|l2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkG3TY5pcWKrdHnvckBw\iCqdX3hckBJUCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkCyN|Uh|ryP M4rmbXNCVkeHUh?=
human RS4-11 cell NEjLVFRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGr6Z4VKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM1NjF6ME[g{txO MYrTRW5ITVJ?
human BEN cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYDRU2JzUW6qaXLpeIlwdiCxZjDoeY1idiCERV6gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOk45OTh5IN88US=> M{HyVHNCVkeHUh?=
human CCRF-CEM cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4fnRWlvcGmkaYTpc44hd2ZiaIXtZY4hS0OURj3DSW0h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Py55N{K5JO69VQ>? NWPYbGN1W0GQR1XS
human EW-13 cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NV\EcVhJUW6qaXLpeIlwdiCxZjDoeY1idiCHVz2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM6NjB2OEGg{txO MYnTRW5ITVJ?
human NCI-H2228 cell M1\pfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkKyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQzNjhzODFOwG0> NVLBO4pCW0GQR1XS
human KE-37 cell NFnwZnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFfFZ5pKdmirYnn0bY9vKG:oIHj1cYFvKEuHLUO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFMvOjlzNTFOwG0> NHnFcZVUSU6JRWK=
human A4-Fuk cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGE1NU[3azDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS1MlA{PjRizszN NWOwN41IW0GQR1XS
human MZ1-PC cell NWX4TZI{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mkj0TY5pcWKrdHnvckBw\iCqdX3hckBOYjFvUFOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20OU46QDh6IN88US=> NVX2[IVwW0GQR1XS
human MLMA cell NXzKZ4V3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mm\6TY5pcWKrdHnvckBw\iCqdX3hckBOVE2DIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NE[uN|I6OiEQvF2= MnrTV2FPT0WU
human EB-3 cell M3q1S2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJcohq[mm2aX;uJI9nKGi3bXHuJGVDNTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12OT61O|kzKM7:TR?= MVPTRW5ITVJ?
human 293T cells  M3\Qe2Z2dmO2aX;uJIF{e2G7 NGHpN2szNjVibV2= M4rJOWlvcGmkaYTpc44hd2ZiSl3KSFIudWWmaXH0[YQhUDONM{[g[IVu\XSqeXzheIlwdiCrbjDoeY1idiB{OUPUJINmdGy|IHH0JFIvPSCvTR?= NHXISWcyQTN3OUG2Oy=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
HIF-1α; 

PubMed: 29555474     


DMOG time-dependently increases the expression of HIF-1α. AML12 cells were treated with DMOG for indicated time. Protein level of HIF-1α was detected by Western blotting. β-actin was used as internal control. Signal intensity was analyzed using Image J software and expressed as mean ± SEM. Shown is the representative of at least 3 repeat experiments. *P < 0.05 v. s. DMSO group.

p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM; 

PubMed: 22797300     


HeLa cells were pretreated with DMOG for 4 hours, followed by NCS treatment as indicated. Western blots were performed with indicated antibodies. 

WNT11 / HIF-2α ; 

PubMed: 26861754     


(E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) and 4T1 cells (F).

29555474 22797300 26861754
Immunofluorescence
HIF1α; 

PubMed: 28955984     


HeLa cells grown in the presence or absence of 1 mM DMOG were immunostained to visualize HiF1α localization. 

28955984
In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]

Protocol

Cell Research:

[3]

- Collapse
  • Cell lines: VSMC cells
  • Concentrations: ~100 μM
  • Incubation Time: 72-96 hours
  • Method:

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Mice (C57Bl6)
  • Formulation: 8 mg/mouse
  • Dosages: 8 mg in 0.5 ml saline
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 175.14
Formula

C6H9NO5

CAS No. 89464-63-1
Storage powder
in solvent
Synonyms Dimethyloxalylglycine

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID