DMOG

Catalog No.S7483 Synonyms: Dimethyloxalylglycine

For research use only.

DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.

DMOG Chemical Structure

CAS No. 89464-63-1

Selleck's DMOG has been cited by 28 publications

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Biological Activity

Description DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.
Targets
HIF prolyl hydroxylase [1]
()
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoWzTY5pcWKrdHnvckBw\iCqdX3hckBUWiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwNUe5OFYh|ryP M3L4fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzOzB7Lze+V2FPT0WUPD;hQi=>
human LB996-RCC cell NHfDUohIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF7SeGFKdmirYnn0bY9vKG:oIHj1cYFvKEyEOUm2MXJESyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTVwNki3O{DPxE1? M{PTSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzOzB7Lze+V2FPT0WUPD;hQi=>
human LOUCY cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGKz[HpKdmirYnn0bY9vKG:oIHj1cYFvKEyRVVPZJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOzR7MzFOwG0> NV:xcpg5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human KS-1 cell M1\Uc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkLJTY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlU2PyEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human U-698-M cell NUXQSXFST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NELsdYlKdmirYnn0bY9vKG:oIHj1cYFvKFVvNkm4MW0h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy5|M{C2JO69VQ>? NETvcGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human A3-KAW cell NEHwPWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4nUPWlvcGmkaYTpc44hd2ZiaIXtZY4hSTNvS1HXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQDF5NDFOwG0> NEfrSYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human BL-70 cell NUO5VpdVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHq4O4FKdmirYnn0bY9vKG:oIHj1cYFvKEKOLUewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQTR{MzFOwG0> MnrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVI{ODlxJ{7TRW5ITVJ:L3G+
human DMS-273 cell MoHSS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULjWGUxUW6qaXLpeIlwdiCxZjDoeY1idiCGTWOtNlc{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTVwOU[1JO69VQ>? NUXPfnJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human CAL-39 cell NUjyW4tsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4\z[GlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvOzJ5NzFOwG0> NFXnN3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human KYSE-410 cell NH;RWmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1G0UGlvcGmkaYTpc44hd2ZiaIXtZY4hU1mVRT20NVAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOC56MUm3JO69VQ>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human YT cell NWnwO2FST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NELN[VZKdmirYnn0bY9vKG:oIHj1cYFvKFmWIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuN|k5PCEQvF2= M3X2NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzOzB7Lze+V2FPT0WUPD;hQi=>
human LC-2-ad cell M3rlWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHfVN5ZKdmirYnn0bY9vKG:oIHj1cYFvKEyFLUKtZYQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPC53NUezJO69VQ>? Mn[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVI{ODlxJ{7TRW5ITVJ:L3G+
human COR-L105 cell NUX2ZZZnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHLLR5ZKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNVA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjZwNESzOkDPxE1? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human CAL-12T cell NWfYRldYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1zMmSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOk42PTB{IN88US=> MkXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVI{ODlxJ{7TRW5ITVJ:L3G+
human KU812 cell MnjpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoXXTY5pcWKrdHnvckBw\iCqdX3hckBMXThzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK4MlQ2PzlizszN MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human OPM-2 cell NV\H[FM3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUfJcohq[mm2aX;uJI9nKGi3bXHuJG9RVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuPFIzPyEQvF2= NInNTZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human SW982 cell M1uyPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2LkUmlvcGmkaYTpc44hd2ZiaIXtZY4hW1d7OEKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zN{44ODB5IN88US=> NXWzepdWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human HH cell NUPrVJhFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYOxNlc4UW6qaXLpeIlwdiCxZjDoeY1idiCKSDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlAzOzVizszN M1zJT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzOzB7Lze+V2FPT0WUPD;hQi=>
human RS4-11 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2D4TWlvcGmkaYTpc44hd2ZiaIXtZY4hWlN2LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOThyNjFOwG0> NHXmNoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human BEN cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3fpbmlvcGmkaYTpc44hd2ZiaIXtZY4hSkWQIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{[uPFE5PyEQvF2= NWDzN3psRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human CCRF-CEM cell Mn;TS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVLwUpFFUW6qaXLpeIlwdiCxZjDoeY1idiCFQ2LGMWNGVSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN5Lke3Nlkh|ryP NHzIPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human EW-13 cell Mk\1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEXqT5VKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvODR6MTFOwG0> MkL4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVI{ODlxJ{7TRW5ITVJ:L3G+
human NCI-H2228 cell M2PpPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NW\TPWJuUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIzOjhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Mj64NVgh|ryP MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human KE-37 cell NUXUOZJST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUOyfGREUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ{NjJ7MUWg{txO NV7adGpnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human A4-Fuk cell MljoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NV2yTXJ{UW6qaXLpeIlwdiCxZjDoeY1idiCDND3GeYsh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PS5yM{[0JO69VQ>? NW\s[oJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQTJ|MEmvK|5USU6JRWK8M4E,
human MZ1-PC cell M{jaVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{PvfWlvcGmkaYTpc44hd2ZiaIXtZY4hVVpzLWDDJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFUvQTh6ODFOwG0> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlMxQS9pPmPBUmdGWjxxYU6=
human MLMA cell MofLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWfGXIdxUW6qaXLpeIlwdiCxZjDoeY1idiCPTF3BJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFYvOzJ7MjFOwG0> NFXnfGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human EB-3 cell MlnDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHXwSWVKdmirYnn0bY9vKG:oIHj1cYFvKEWELUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20PU42Pzl{IN88US=> NFXK[Hk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjNyOT:nQnNCVkeHUkyvZV4>
human 293T cells  MWjGeY5kfGmxbjDhd5NigQ>? MmO1Nk42KG2P NEi3WoRKdmirYnn0bY9vKG:oIFrNTmQzNW2nZHnheIVlKEh|S{O2JIRmdWW2aInsZZRqd25iaX6gbJVu[W5iMkmzWEBk\WyuczDheEAzNjVibV2= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN3OUG2O{c,OTl|NUmxOlc9N2F-
Assay
Methods Test Index PMID
Western blot HIF-1α ; p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM ; WNT11 / HIF-2α 29555474 22797300 26861754
Immunofluorescence HIF1α 28955984
In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: VSMC cells
  • Concentrations: ~100 μM
  • Incubation Time: 72-96 hours
  • Method:

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.

Animal Research:

[2]

  • Animal Models: Mice (C57Bl6)
  • Dosages: 8 mg in 0.5 ml saline
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 35 mg/mL
(199.84 mM)
Water 35 mg/mL
(199.84 mM)
Ethanol '35 mg/mL

Chemical Information

Molecular Weight 175.14
Formula

C6H9NO5

Density 1.246 g/mL
CAS No. 89464-63-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC(=O)CNC(=O)C(=O)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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