DMOG

For research use only.

Catalog No.S7483 Synonyms: Dimethyloxalylglycine

15 publications

DMOG Chemical Structure

CAS No. 89464-63-1

DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 120 In stock
USD 97 In stock
USD 194 In stock
USD 327 In stock
USD 837 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's DMOG has been cited by 15 publications

5 Customer Reviews

  • Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

    Hepatology, 2018, 68(6):2359-2375. DMOG purchased from Selleck.

  • Western blot analysis of the indicated proteins after the treatment with DMOG (K).

    Theranostics, 2017, 7(13): 3293-3305. DMOG purchased from Selleck.

  • Western blot analysis of the indicated proteins after the treatment with DMOG.

    Theranostics, 2017, 7(13):3293-3305. DMOG purchased from Selleck.

  • OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

    Clin Exp Immunol, 2018, doi:10.1111/cei.13227. DMOG purchased from Selleck.

  • (A) Representative western blot images for appoptosin and HIF-1α protein expression in control, DMOG and 1,4-DPCA groups. (B) Protein expression levels of appoptosin and HIF-1α in control, DMOG and 1,4-DPCA groups were quantified by densitometric analysis.

    Mol Med Rep, 2018, 17(5):7149-7155. DMOG purchased from Selleck.

Purity & Quality Control

Choose Selective Hydroxylase Inhibitors

Biological Activity

Description DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.
Targets
HIF prolyl hydroxylase [1]
()
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell M4jOVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIDrSIJKdmirYnn0bY9vKG:oIHj1cYFvKFOUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;ND61O|k1PiEQvF2= NH7FVZhUSU6JRWK=
human LB996-RCC cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmDXTY5pcWKrdHnvckBw\iCqdX3hckBNSjl7Nj3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjZ6N{eg{txO MWnTRW5ITVJ?
human LOUCY cell MmTPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH7jcYdKdmirYnn0bY9vKG:oIHj1cYFvKEyRVVPZJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOzR7MzFOwG0> M3n3b3NCVkeHUh?=
human KS-1 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUPqOYl[UW6qaXLpeIlwdiCxZjDoeY1idiCNUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvOjV3NzFOwG0> NYP1SVBVW0GQR1XS
human U-698-M cell NV[4UmxPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJHUuPjl6LV2gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4{OzB4IN88US=> NWroUGt3W0GQR1XS
human A3-KAW cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NY\R[ZA{UW6qaXLpeIlwdiCxZjDoeY1idiCDMz3LRXch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS56MUe0JO69VQ>? NFuwXIZUSU6JRWK=
human BL-70 cell NV6xXZpwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH;5d5NKdmirYnn0bY9vKG:oIHj1cYFvKEKOLUewJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQTR{MzFOwG0> M2H3WHNCVkeHUh?=
human DMS-273 cell MnTES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXfJcohq[mm2aX;uJI9nKGi3bXHuJGROWy1{N{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU46PjVizszN MVHTRW5ITVJ?
human CAL-39 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{SwcGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUO5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVkvOzJ5NzFOwG0> NFjLSGZUSU6JRWK=
human KYSE-410 cell NYSyWFRvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml;vTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUSxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjhzOUeg{txO NIXQbZRUSU6JRWK=
human YT cell M{S3RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mon2TY5pcWKrdHnvckBw\iCqdX3hckB[XCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJ|LkO5PFQh|ryP MkPOV2FPT0WU
human LC-2-ad cell M4rWNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUTJcohq[mm2aX;uJI9nKGi3bXHuJGxENTJvYXSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE42PTd|IN88US=> NES2d|RUSU6JRWK=
human COR-L105 cell M165OGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mlf0TY5pcWKrdHnvckBw\iCqdX3hckBEV1JvTEGwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI3NjR2M{[g{txO MX3TRW5ITVJ?
human CAL-12T cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17CU2lvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUGyWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI3NjV3MEKg{txO MXHTRW5ITVJ?
human KU812 cell NFHwcpRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{H0fGlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE41PTd7IN88US=> NEDBeFlUSU6JRWK=
human OPM-2 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX3MWFhGUW6qaXLpeIlwdiCxZjDoeY1idiCRUF2tNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI5Njh{Mkeg{txO MofyV2FPT0WU
human SW982 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{OuO|AxPyEQvF2= NUfuWWVvW0GQR1XS
human HH cell NV3tfZFST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NETtd2VKdmirYnn0bY9vKG:oIHj1cYFvKEiKIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuNFI{PSEQvF2= MkTyV2FPT0WU
human RS4-11 cell NIjwfWRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUW0R5p{UW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{PC5zOEC2JO69VQ>? NWWyZVN3W0GQR1XS
human BEN cell NWrNT5lqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFPTdZZKdmirYnn0bY9vKG:oIHj1cYFvKEKHTjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO2MlgyQDdizszN MV7TRW5ITVJ?
human CCRF-CEM cell M17ER2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1LzUWlvcGmkaYTpc44hd2ZiaIXtZY4hS0OURj3DSW0h[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Py55N{K5JO69VQ>? NHTOSm5USU6JRWK=
human EW-13 cell MnnuS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEG4UZFKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvODR6MTFOwG0> NWXvT2t2W0GQR1XS
human NCI-H2228 cell NUHPeW1ST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3XGc2lvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNlI5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PDJwOEG4JO69VQ>? NFvMXXdUSU6JRWK=
human KE-37 cell NWDwbnQ1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2KyUWlvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20N{4zQTF3IN88US=> NHjmbWNUSU6JRWK=
human A4-Fuk cell M2jGPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGLtd2tKdmirYnn0bY9vKG:oIHj1cYFvKEF2LV\1b{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ2NjB|NkSg{txO M2XxeHNCVkeHUh?=
human MZ1-PC cell NGCxeWVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV[1cYtCUW6qaXLpeIlwdiCxZjDoeY1idiCPWkGtVGMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PS57OEi4JO69VQ>? MkHuV2FPT0WU
human MLMA cell NUP3Tm5zT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVPJcohq[mm2aX;uJI9nKGi3bXHuJG1NVUFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Nj6zNlkzKM7:TR?= Mn\pV2FPT0WU
human EB-3 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17PeWlvcGmkaYTpc44hd2ZiaIXtZY4hTUJvMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS5MlU4QTJizszN NVPWdYN[W0GQR1XS
human 293T cells  NUf6bGZoTnWwY4Tpc44h[XO|YYm= NYPXTIliOi53IH3N MVzJcohq[mm2aX;uJI9nKEqPSlSyMY1m\GmjdHXkJGg{UzN4IHTlcYV1cHmuYYTpc44hcW5iaIXtZY4hOjl|VDDj[YxteyCjdDCyMlUhdU1? M3rwb|E6OzV7MU[3

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
HIF-1α; 

PubMed: 29555474     


DMOG time-dependently increases the expression of HIF-1α. AML12 cells were treated with DMOG for indicated time. Protein level of HIF-1α was detected by Western blotting. β-actin was used as internal control. Signal intensity was analyzed using Image J software and expressed as mean ± SEM. Shown is the representative of at least 3 repeat experiments. *P < 0.05 v. s. DMSO group.

p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM; 

PubMed: 22797300     


HeLa cells were pretreated with DMOG for 4 hours, followed by NCS treatment as indicated. Western blots were performed with indicated antibodies. 

WNT11 / HIF-2α ; 

PubMed: 26861754     


(E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) and 4T1 cells (F).

29555474 22797300 26861754
Immunofluorescence
HIF1α; 

PubMed: 28955984     


HeLa cells grown in the presence or absence of 1 mM DMOG were immunostained to visualize HiF1α localization. 

28955984
In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]

Protocol

Cell Research:

[3]

- Collapse
  • Cell lines: VSMC cells
  • Concentrations: ~100 μM
  • Incubation Time: 72-96 hours
  • Method:

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Mice (C57Bl6)
  • Dosages: 8 mg in 0.5 ml saline
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol '35 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 175.14
Formula

C6H9NO5

Density 1.246 g/mL
CAS No. 89464-63-1
Storage powder
in solvent
Synonyms Dimethyloxalylglycine
Smiles COC(=O)CNC(=O)C(=O)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Hydroxylase Signaling Pathway Map

Related Hydroxylase Products

Tags: buy DMOG | DMOG supplier | purchase DMOG | DMOG cost | DMOG manufacturer | order DMOG | DMOG distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID