DMOG

Catalog No.S7483 Synonyms: Dimethyloxalylglycine

DMOG Chemical Structure

Molecular Weight(MW): 175.14

DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase.

Size Price Stock Quantity  
In DMSO USD 120 In stock
USD 97 In stock
USD 194 In stock
USD 327 In stock
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Cited by 5 Publications

5 Customer Reviews

  • Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

    Hepatology, 2018, 68(6):2359-2375. DMOG purchased from Selleck.

    Western blot analysis of the indicated proteins after the treatment with DMOG (K).

    Theranostics, 2017, 7(13): 3293-3305. DMOG purchased from Selleck.

  • Western blot analysis of the indicated proteins after the treatment with DMOG.

    Theranostics, 2017, 7(13):3293-3305. DMOG purchased from Selleck.

    OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

    Clin Exp Immunol, 2018, doi:10.1111/cei.13227. DMOG purchased from Selleck.

  • (A) Representative western blot images for appoptosin and HIF-1α protein expression in control, DMOG and 1,4-DPCA groups. (B) Protein expression levels of appoptosin and HIF-1α in control, DMOG and 1,4-DPCA groups were quantified by densitometric analysis.

    Mol Med Rep, 2018, 17(5):7149-7155. DMOG purchased from Selleck.

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Biological Activity

Description DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase.
Targets
HIF prolyl hydroxylase [1]
()
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell NFrLXFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mnv5TY5pcWKrdHnvckBw\iCqdX3hckBUWiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTRwNUe5OFYh|ryP NXfyNlVtW0GQR1XS
human LB996-RCC cell M{LxWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYXNVVdtUW6qaXLpeIlwdiCxZjDoeY1idiCOQkm5Ok1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lk[4O|ch|ryP MYnTRW5ITVJ?
human LOUCY cell M3flZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXHJcohq[mm2aX;uJI9nKGi3bXHuJGxQXUO\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuN|Q6OyEQvF2= MknCV2FPT0WU
human KS-1 cell MnP2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlS0TY5pcWKrdHnvckBw\iCqdX3hckBMWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlU2PyEQvF2= MYLTRW5ITVJ?
human U-698-M cell M3TjVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4XMXWlvcGmkaYTpc44hd2ZiaIXtZY4hXS14OUitUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjN|ME[g{txO NFTz[VdUSU6JRWK=
human A3-KAW cell NVfaUopqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{\rXWlvcGmkaYTpc44hd2ZiaIXtZY4hSTNvS1HXJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvQDF5NDFOwG0> M{X4XXNCVkeHUh?=
human BL-70 cell NFj1W2FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVXJcohq[mm2aX;uJI9nKGi3bXHuJGJNNTdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuPVQzOyEQvF2= M3n6WHNCVkeHUh?=
human DMS-273 cell M2HLOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIjac2xKdmirYnn0bY9vKG:oIHj1cYFvKESPUz2yO|Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS57NkWg{txO M2XzR3NCVkeHUh?=
human CAL-39 cell M1\FR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWroXHNLUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytN|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yQS5|Mke3JO69VQ>? NUXpeW9mW0GQR1XS
human KYSE-410 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnrETY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUSxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjhzOUeg{txO M4TIUHNCVkeHUh?=
human YT cell MmnDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mlu0TY5pcWKrdHnvckBw\iCqdX3hckB[XCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJ|LkO5PFQh|ryP NXG5UpQ5W0GQR1XS
human LC-2-ad cell NHrpUpJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVXFTnVmUW6qaXLpeIlwdiCxZjDoeY1idiCOQz2yMYFlKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjRwNUW3N{DPxE1? MUTTRW5ITVJ?
human COR-L105 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH;vellKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNVA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjZwNESzOkDPxE1? NV3HW4N7W0GQR1XS
human CAL-12T cell NEXVbWFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYLJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1zMmSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOk42PTB{IN88US=> M{\YT3NCVkeHUh?=
human KU812 cell MXnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlzGTY5pcWKrdHnvckBw\iCqdX3hckBMXThzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK4MlQ2PzlizszN M3LkR3NCVkeHUh?=
human OPM-2 cell M{fiUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF3KXpdKdmirYnn0bY9vKG:oIHj1cYFvKE:STT2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlgvQDJ{NzFOwG0> NE\yZXJUSU6JRWK=
human SW982 cell M{Hjb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHz0S|BKdmirYnn0bY9vKG:oIHj1cYFvKFOZOUiyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|MvPzByNzFOwG0> M3XzNXNCVkeHUh?=
human HH cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3rlbGlvcGmkaYTpc44hd2ZiaIXtZY4hUEhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|ND6wNlM2KM7:TR?= M3;xS3NCVkeHUh?=
human RS4-11 cell MnvvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUPJcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlE5ODZizszN MVXTRW5ITVJ?
human BEN cell M2Lub2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVrvTmVJUW6qaXLpeIlwdiCxZjDoeY1idiCERV6gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOk45OTh5IN88US=> M1\sTHNCVkeHUh?=
human CCRF-CEM cell NGqzXVJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIGwXmRKdmirYnn0bY9vKG:oIHj1cYFvKEOFUl[tR2VOKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzdwN{eyPUDPxE1? M2O5S3NCVkeHUh?=
human EW-13 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEfj[nVKdmirYnn0bY9vKG:oIHj1cYFvKEWZLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvODR6MTFOwG0> NF71do9USU6JRWK=
human NCI-H2228 cell NFTDcIxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXPJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkKyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQzNjhzODFOwG0> Mlu3V2FPT0WU
human KE-37 cell NXvXO4tqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{\tW2lvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20N{4zQTF3IN88US=> NE\O[4pUSU6JRWK=
human A4-Fuk cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmPDTY5pcWKrdHnvckBw\iCqdX3hckBCPC2IdXugZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20OU4xOzZ2IN88US=> NHvoUXlUSU6JRWK=
human MZ1-PC cell NHyydYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX;XdXZTUW6qaXLpeIlwdiCxZjDoeY1idiCPWkGtVGMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PS57OEi4JO69VQ>? M{ewOHNCVkeHUh?=
human MLMA cell NHToOWxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkHRTY5pcWKrdHnvckBw\iCqdX3hckBOVE2DIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NE[uN|I6OiEQvF2= MXLTRW5ITVJ?
human EB-3 cell NYK1N2FuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHnWV4hKdmirYnn0bY9vKG:oIHj1cYFvKEWELUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20PU42Pzl{IN88US=> Mm\JV2FPT0WU
human 293T cells  MVzGeY5kfGmxbjDhd5NigQ>? M13oe|IvPSCvTR?= M{TYS2lvcGmkaYTpc44hd2ZiSl3KSFIudWWmaXH0[YQhUDONM{[g[IVu\XSqeXzheIlwdiCrbjDoeY1idiB{OUPUJINmdGy|IHH0JFIvPSCvTR?= NEjlTYwyQTN3OUG2Oy=>

... Click to View More Cell Line Experimental Data

In vivo DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]

Protocol

Cell Research:

[3]

+ Expand
  • Cell lines: VSMC cells
  • Concentrations: ~100 μM
  • Incubation Time: 72-96 hours
  • Method:

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Mice (C57Bl6)
  • Formulation: 8 mg/mouse
  • Dosages: 8 mg in 0.5 ml saline
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 175.14
Formula

C6H9NO5

CAS No. 89464-63-1
Storage powder
in solvent
Synonyms Dimethyloxalylglycine

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID