DMOG

Catalog No.S7483 Synonyms: Dimethyloxalylglycine

DMOG Chemical Structure

Molecular Weight(MW): 175.14

DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus.

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In DMSO USD 120 In stock
USD 97 In stock
USD 194 In stock
USD 327 In stock
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Cited by 6 Publications

5 Customer Reviews

  • Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

    Hepatology, 2018, 68(6):2359-2375. DMOG purchased from Selleck.

    Western blot analysis of the indicated proteins after the treatment with DMOG (K).

    Theranostics, 2017, 7(13): 3293-3305. DMOG purchased from Selleck.

  • Western blot analysis of the indicated proteins after the treatment with DMOG.

    Theranostics, 2017, 7(13):3293-3305. DMOG purchased from Selleck.

    OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

    Clin Exp Immunol, 2018, doi:10.1111/cei.13227. DMOG purchased from Selleck.

  • (A) Representative western blot images for appoptosin and HIF-1α protein expression in control, DMOG and 1,4-DPCA groups. (B) Protein expression levels of appoptosin and HIF-1α in control, DMOG and 1,4-DPCA groups were quantified by densitometric analysis.

    Mol Med Rep, 2018, 17(5):7149-7155. DMOG purchased from Selleck.

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Biological Activity

Description DMOG is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus.
Targets
HIF prolyl hydroxylase [1]
()
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human SR cell NEnSTHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYDJcohq[mm2aX;uJI9nKGi3bXHuJHNTKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PC53N{m0OkDPxE1? Mof2V2FPT0WU
human LB996-RCC cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUDkfI9oUW6qaXLpeIlwdiCxZjDoeY1idiCOQkm5Ok1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD13Lk[4O|ch|ryP MYTTRW5ITVJ?
human LOUCY cell NWDhTmQzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGxQXUO\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUCuN|Q6OyEQvF2= NEnPb5VUSU6JRWK=
human KS-1 cell M{n4cWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVjJcohq[mm2aX;uJI9nKGi3bXHuJGtUNTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMT6yOVU4KM7:TR?= NUTqOoQ1W0GQR1XS
human U-698-M cell NFPVSHFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{TsVmlvcGmkaYTpc44hd2ZiaIXtZY4hXS14OUitUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjN|ME[g{txO Ml2xV2FPT0WU
human A3-KAW cell NEWzb2JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NW\Kdo5DUW6qaXLpeIlwdiCxZjDoeY1idiCDMz3LRXch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPS56MUe0JO69VQ>? Mm\GV2FPT0WU
human BL-70 cell MnXwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYLJcohq[mm2aX;uJI9nKGi3bXHuJGJNNTdyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuPVQzOyEQvF2= MVzTRW5ITVJ?
human DMS-273 cell NGq3TlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGROWy1{N{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOU46PjVizszN NIjZVpVUSU6JRWK=
human CAL-39 cell MmnES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG5MlMzPzdizszN M1fwT3NCVkeHUh?=
human KYSE-410 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmHzTY5pcWKrdHnvckBw\iCqdX3hckBMYVOHLUSxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIxNjhzOUeg{txO M2LaV3NCVkeHUh?=
human YT cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKGi3bXHuJHlVKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjNwM{m4OEDPxE1? M4na[XNCVkeHUh?=
human LC-2-ad cell MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVHJcohq[mm2aX;uJI9nKGi3bXHuJGxENTJvYXSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE42PTd|IN88US=> NGXoUlZUSU6JRWK=
human COR-L105 cell M1i5T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHf0NnNKdmirYnn0bY9vKG:oIHj1cYFvKEORUj3MNVA2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjZwNESzOkDPxE1? NVLaVYdJW0GQR1XS
human CAL-12T cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHf5XI9KdmirYnn0bY9vKG:oIHj1cYFvKEODTD2xNnQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPi53NUCyJO69VQ>? NYjCV49QW0GQR1XS
human KU812 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{Lv[GlvcGmkaYTpc44hd2ZiaIXtZY4hU1V6MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE41PTd7IN88US=> M3HPd3NCVkeHUh?=
human OPM-2 cell M4TQXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{jNUWlvcGmkaYTpc44hd2ZiaIXtZY4hV1CPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE45OjJ5IN88US=> MnfMV2FPT0WU
human SW982 cell NHf4NJZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXLJcohq[mm2aX;uJI9nKGi3bXHuJHNYQTh{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{OuO|AxPyEQvF2= MXzTRW5ITVJ?
human HH cell NIC3eYVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MojLTY5pcWKrdHnvckBw\iCqdX3hckBJUCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkCyN|Uh|ryP MmjHV2FPT0WU
human RS4-11 cell M37XbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2mxcmlvcGmkaYTpc44hd2ZiaIXtZY4hWlN2LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOThyNjFOwG0> NWi1fYRIW0GQR1XS
human BEN cell NH7scJlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJGJGViClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN4LkixPFch|ryP NYOwNlZvW0GQR1XS
human CCRF-CEM cell NIPsVVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXmyfG5wUW6qaXLpeIlwdiCxZjDoeY1idiCFQ2LGMWNGVSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN5Lke3Nlkh|ryP MYHTRW5ITVJ?
human EW-13 cell NGrUSo5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4DEZ2lvcGmkaYTpc44hd2ZiaIXtZY4hTVdvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPU4xPDhzIN88US=> M4npNHNCVkeHUh?=
human NCI-H2228 cell NXHETG5JT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPGUmRYUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIzOjhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12Mj64NVgh|ryP NYrsZVl2W0GQR1XS
human KE-37 cell NF[0WFRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXzJcohq[mm2aX;uJI9nKGi3bXHuJGtGNTN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NEOuNlkyPSEQvF2= M3;3dXNCVkeHUh?=
human A4-Fuk cell NF3RW5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2CxTmlvcGmkaYTpc44hd2ZiaIXtZY4hSTRvRoXrJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFUvODN4NDFOwG0> Ml3JV2FPT0WU
human MZ1-PC cell NFL1R4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnvuTY5pcWKrdHnvckBw\iCqdX3hckBOYjFvUFOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME20OU46QDh6IN88US=> MlnxV2FPT0WU
human MLMA cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVfFR3R7UW6qaXLpeIlwdiCxZjDoeY1idiCPTF3BJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFYvOzJ7MjFOwG0> MkT1V2FPT0WU
human EB-3 cell M1;JNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXLJcohq[mm2aX;uJI9nKGi3bXHuJGVDNTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12OT61O|kzKM7:TR?= Mln3V2FPT0WU
human 293T cells  Mn;oSpVv[3Srb36gZZN{[Xl? NH:wTFEzNjVibV2= Mkj2TY5pcWKrdHnvckBw\iCMTVrENk1u\WSrYYTl[EBJO0t|NjDk[Y1mfGi7bHH0bY9vKGmwIHj1cYFvKDJ7M2SgZ4VtdHNiYYSgNk42KG2P NXLVc5llOTl|NUmxOlc>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
HIF-1α; 

PubMed: 29555474     


DMOG time-dependently increases the expression of HIF-1α. AML12 cells were treated with DMOG for indicated time. Protein level of HIF-1α was detected by Western blotting. β-actin was used as internal control. Signal intensity was analyzed using Image J software and expressed as mean ± SEM. Shown is the representative of at least 3 repeat experiments. *P < 0.05 v. s. DMSO group.

p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM; 

PubMed: 22797300     


HeLa cells were pretreated with DMOG for 4 hours, followed by NCS treatment as indicated. Western blots were performed with indicated antibodies. 

WNT11 / HIF-2α ; 

PubMed: 26861754     


(E,F) Induction of Wnt11 by increasing concentrations of DMOG in MDA-MB-231 cells (E) and 4T1 cells (F).

29555474 22797300 26861754
Immunofluorescence
HIF1α; 

PubMed: 28955984     


HeLa cells grown in the presence or absence of 1 mM DMOG were immunostained to visualize HiF1α localization. 

28955984
In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]

Protocol

Cell Research:

[3]

+ Expand
  • Cell lines: VSMC cells
  • Concentrations: ~100 μM
  • Incubation Time: 72-96 hours
  • Method:

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Mice (C57Bl6)
  • Formulation: 8 mg/mouse
  • Dosages: 8 mg in 0.5 ml saline
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 175.14
Formula

C6H9NO5

CAS No. 89464-63-1
Storage powder
in solvent
Synonyms Dimethyloxalylglycine

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID