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Ro 61-8048 Hydroxylase inhibitor

Name: Ro 61-8048
Brand: Selleck Chemicals
SKU: S8172
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S8172

Ro 61-8048 is a high-affinity kynurenine 3-hydroxylase (KMO) inhibitor with IC50 and K_i of 37 nM and 4.8 nM, respectively.

Chemical Structure

Molecular Weight: 421.45

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.95%

99.95

Related Targets	P450 (e.g. CYP17) Dehydrogenase PDE phosphatase PPAR HSP (HSP90) Vitamin Transferase Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase Serine/threonin kinase Lipoxygenase Phospholipase (e.g. PLA) Retinoid Receptor DPP ACE Peroxidases Fatty Acid Synthase FXR HMG-CoA Reductase Carbonic Anhydrase Lipase Decarboxylase DHFR IDO/TDO PCSK9 OXPHOS ROR Acyltransferase
Related Products	DMOG Meldonium 4-Chloro-DL-phenylalanine

Chemical Information, Storage & Stability

Molecular Weight	421.45	Formula	C₁₇H₁₅N₃O₆S₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	199666-03-0	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	COC1=C(C=C(C=C1)S(=O)(=O)NC2=NC(=CS2)C3=CC(=CC=C3)[N+](=O)[O-])OC

Solubility

In vitro
Batch:

DMSO : 84 mg/mL ( (199.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (23.73mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	KMO ^[1] (Cell-free assay) 4.8 nM(Ki) KMO ^[1] (Cell-free assay) 37 nM
In vivo	In gerbils and rats, Ro 61-8048 (30 mg/kg, p.o.) causes kynurenine 3-hydroxylase inhibition. ^[1] In models of focal or global brain ischemia, this compound (40 mg/kg, p.o.) reduce ischemic brain damage. ^[2] In a hamster model of paroxysmal dyskinesia, this chemical (150 mg/kg i.p.) significantly reduces the severity of dystonia without leading to marked central side effects. ^[3] addition, prenatal inhibition of the kynurenine pathway by this compound leads to structural changes in the hippocampus of adult rat offspring. ^[4]
References	https://pubmed.ncbi.nlm.nih.gov/9435907/ https://pubmed.ncbi.nlm.nih.gov/10413032/ https://pubmed.ncbi.nlm.nih.gov/14555184/ https://pubmed.ncbi.nlm.nih.gov/24646396/

Tech Support

Handling Instructions

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