For research use only.

Catalog No.S2425 Synonyms: Acetovanillone

15 publications

Apocynin Chemical Structure

CAS No. 498-02-2

Apocynin (Acetovanillone) is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.

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Selleck's Apocynin has been cited by 15 publications

4 Customer Reviews

  • NOX inhibition attenuated thyroid morphologic changes of rats. (a) A representative figure from control group; (b) a representative figure from PCB group; (c) a representative figure from PCB + APO group; (d) a representative figure from APO group; (e) histological scores of thyroid tissue. ∗P < 0.05 versus control group. #P < 0.05 versus PCB group (original magnification ×200).

    Int J Endocrinol, 2016, 2016:8354745.. Apocynin purchased from Selleck.

  • (A) Fluorescent images of the cytoskeleton (red) and vinculin (green, a protein in focal adhesion plaques) show the cell adhesion and morphology. The actin filaments (red) of VEC were stained with Rhodamine-phalloidin and cell nuclei (blue) were stained with DAPI. Arrowheads indicate the twisted cytoskeleton and arrows indicate the intracellular vacuoles. The image in white box is an enlarged view of three cells with twisted cytoskeleton. Scale bar: 50 μm in the above and 20 μm in the below.

    Acta Biomater, 2018, 73:470-487. Apocynin purchased from Selleck.

  • RAW264.7 cells were pretreated with apocynin (500 μM) for 2 h and then exposed to LPS (100 ng/ml) for 5 min. After treatment, cells were washed with PBS and the fluorescence intensity was measured by flow cytometry (c). RAW264.7 cells were incubated with apocynin (500 μM) for 2 h and then stimulated with 100 ng/ml LPS for 16 h (d). Cells were harvested, and equal amounts of whole cell lysates were analyzed by western blotting with anti-iNOS or anti-COX-2 antibody. Data were mean ± SD values of three independent experiments. ∗P < 0.05 and ∗∗P < 0.01 versus LPS-treated group.

    Oxid Med Cell Longev, 2017, 2017:9738745. Apocynin purchased from Selleck.

  • Treatment of rats with apocynin attenuated pancreatic morphological alterations. Representative images from the (A) sham operation (SO) group; (B) severe acute pancreatitis (SAP) group; (C) drug control (APO-CON) group; and (D) apocynin (APO) group (original magnification, ×200). (E) Pancreatic histological scores. Results are presented as the mean ± standard deviation. *P<0.05 vs. SO group; #P<0.05 vs. SAP group.

    Mol Med Rep, 2016, 14(4):3525-34.. Apocynin purchased from Selleck.

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Choose Selective NADPH-oxidase Inhibitors

Biological Activity

Description Apocynin (Acetovanillone) is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.
NADPH-oxidase [1]
(Cell-free assay)
10 μM
In vitro

Apocynin is a naturally occurring methoxy-substituted catechol used as an inhibitor of NADPH-oxidase. Apocynin prevents serine phosphorylation of p47phox and blocks its association with gp91phox, thus blunting NADPH oxidase activation. [2] Apocynin can decrease the production of superoxide (O(2)(-)) from activated neutrophils and macrophages. Apocynin, after metabolic conversion, inhibits the assembly of NADPH-oxidase that is responsible for reactive oxygen species (ROS) production. [3]

In vivo Apocynin has anti-inflammatory activity in a variety of cell and animal models of inflammation. Apocynin attenuates the TNF-α and IL-1β production and iNOS expression in the lung of carrageenan-treated mice. [4] Apocynin exerts beneficial effects in a mice model of spinal cord injury. Apocynin reduces (1) the degree of spinal cord damage, (2) neutrophils infiltration, (3) ICAM-1 and P-selectin expression, (4) PAR and nitrotyrosine formation, (5) IκB-α degradation, (6) NF-κB activation, (7) pro-inflammatory cytokines production (TNF-α and IL-1β), (8) apoptosis (TUNEL staining, FAS ligand expression, Bax and Bcl-2 expression) and (9) MAPK activation (P-38 and phospho-JNK). [5] LD50: Mice 9g/kg (i.g.). [3]


Solubility (25°C)

In vitro DMSO 33 mg/mL (198.59 mM)
Ethanol 33 mg/mL (198.59 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 166.17


CAS No. 498-02-2
Storage powder
in solvent
Synonyms Acetovanillone
Smiles CC(=O)C1=CC(=C(C=C1)O)OC

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID