Apocynin (NSC 2146)

For research use only.

Catalog No.S2425 Synonyms: Acetovanillone, NSC 209524

24 publications

Apocynin (NSC 2146) Chemical Structure

CAS No. 498-02-2

Apocynin (NSC 2146, Acetovanillone, NSC 209524) is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.

Selleck's Apocynin (NSC 2146) has been cited by 24 publications

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Biological Activity

Description Apocynin (NSC 2146, Acetovanillone, NSC 209524) is a selective NADPH-oxidase inhibitor with IC50 of 10 μM.
NADPH-oxidase [1]
(Cell-free assay)
10 μM
In vitro

Apocynin is a naturally occurring methoxy-substituted catechol used as an inhibitor of NADPH-oxidase. Apocynin prevents serine phosphorylation of p47phox and blocks its association with gp91phox, thus blunting NADPH oxidase activation. [2] Apocynin can decrease the production of superoxide (O(2)(-)) from activated neutrophils and macrophages. Apocynin, after metabolic conversion, inhibits the assembly of NADPH-oxidase that is responsible for reactive oxygen species (ROS) production. [3]

In vivo Apocynin has anti-inflammatory activity in a variety of cell and animal models of inflammation. Apocynin attenuates the TNF-α and IL-1β production and iNOS expression in the lung of carrageenan-treated mice. [4] Apocynin exerts beneficial effects in a mice model of spinal cord injury. Apocynin reduces (1) the degree of spinal cord damage, (2) neutrophils infiltration, (3) ICAM-1 and P-selectin expression, (4) PAR and nitrotyrosine formation, (5) IκB-α degradation, (6) NF-κB activation, (7) pro-inflammatory cytokines production (TNF-α and IL-1β), (8) apoptosis (TUNEL staining, FAS ligand expression, Bax and Bcl-2 expression) and (9) MAPK activation (P-38 and phospho-JNK). [5] LD50: Mice 9g/kg (i.g.). [3]


Solubility (25°C)

In vitro DMSO 33 mg/mL (198.59 mM)
Ethanol 33 mg/mL (198.59 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 166.17


CAS No. 498-02-2
Storage powder
in solvent
Synonyms Acetovanillone, NSC 209524
Smiles CC(=O)C1=CC(=C(C=C1)O)OC

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID