2-Acetylphenothiazine (ML171)

Catalog No.S5304 Synonyms: 2-APT

For research use only.

2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.

2-Acetylphenothiazine (ML171) Chemical Structure

CAS No. 6631-94-3

Selleck's 2-Acetylphenothiazine (ML171) has been cited by 2 Publications

Purity & Quality Control

Choose Selective NADPH-oxidase Inhibitors

Biological Activity

Description 2-Acetylphenothiazine (ML171, 2-APT) is a potent and selective inhibitor of NADPH-oxidase with IC50s of 0.25 μM, 5 μM, 3μM, 5 μM and 5.5 μM for NOX1, NOX2, NOX3, NOX4 and xanthine oxidase, respectively.
Targets
NOX1 [1]
(HEK293)
NOX3 [1]
(HEK293)
NOX2 [1]
(HEK293)
NOX4 [1]
(HEK293)
xanthine oxidase [1]
(HEK293)
0.25 μM 3 μM 5 μM 5 μM 5.5 μM
In vitro

ML171 is a potent and selective inhibitor of NOX1, with an IC50 of 129–156 nM in cell-based assays. ML171 is not cytotoxic, and is selective among NOX family members NOX2, NOX3, and NOX4, as well as against xanthine oxidase, another cellular source of ROS. In addition, ML171 is highly effective in inhibiting the cellular production of invadopodia in a human colon cancer cell line. It strongly blocks ROS generation in HT29 cells (IC50HT29 = 0.129 μM)[2]. ML171 does not inhibit mitochondrial ROS production[3].

Protocol (from reference)

Cell Research:

[3]

  • Cell lines: DLD1 cells
  • Concentrations: 10 μM
  • Incubation Time: 1 h
  • Method:

    DLD1 cells were plated on glass coverslips and after 24hrs cells were transfected with active SrcYF or empty vector (mock). 48hrs after transfection, cells were treated as indicated for 1hr with 10 μM of ML171, or with DMSO or 10 μM DPI. Afterwards, cells were fixed and stained and visualized by confocal miscroscopy.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 48 mg/mL
(198.91 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 241.31
Formula

C14H11NOS

CAS No. 6631-94-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)C1=CC2=C(C=C1)SC3=CC=CC=C3N2

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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