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Pemetrexed (LY-231514) disodium

Name: Pemetrexed (LY-231514) disodium
Brand: Selleck Chemicals
SKU: S1135
Rating: 5 (39 reviews)

Catalog No.S1135

For research use only.

Pemetrexed (LY-231514) disodium is a novel antifolate and antimetabolite for TS, DHFR and GARFT with K_i of 1.3 nM, 7.2 nM and 65 nM in cell-free assays, respectively. Pemetrexed induces autophagy and apoptosis.

Pemetrexed (LY-231514) disodium Chemical Structure

CAS No. 150399-23-8

Selleck's Pemetrexed (LY-231514) disodium has been cited by 39 publications

Purity & Quality Control

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DHFR Signaling Pathway Map

Biological Activity

Description

Targets

TS ^[1] (Cell-free assay)	DHFR ^[1] (Cell-free assay)	GARFT ^[1] (Cell-free assay)
1.3 nM(Ki)	7.2 nM(Ki)	65 nM(Ki)

In vitro

Pemetrexed disodium shows the antiproliferative activity in CCRF-CEM leukemia, GC3/C1 colon carcinoma, and HCT-8 ileocecal carcinoma cells with IC50 of 25 nM, 34 nM and 220 nM, respectively. ^[1] A recent study shows that cisplatin plus Pemetrexed combined with SOCS-1 gene delivery shows the antitumor effect by inhibition of cell proliferation, invasiveness, and induction of apoptosis in MPM cells infected with adenovirus-expressing SOCS-1 vector. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

PANC-1	NWH6bWE6TnWwY4Tpc44hSXO\|YYm=	NXXYXYlVQDRizszN	NHe0[XIzPCCq		NFTiT5VmdmijbnPld{BGT0[ULDDISXI{KGGwZDDBT3QheGixc4Doc5J6dGG2aX;uJIxmfmWucx?=	MlzZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{e2NFcoRjJ{OUe3OlA4RC:jPh?=
BXPC-3	MmniSpVv[3Srb36gRZN{[Xl?	M4DmTFM6Njh4IDFOwG0>	M17EcVI1KGh?		M{LYbYVvcGGwY3XzJGVITlJuIFjFVlMh[W6mIFHLWEBxcG:\|cHjvdplt[XSrb36gcIV3\Wy\|	M1XOT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUe3OlA4Lz5{Mkm3O\|YxPzxxYU6=
PANC-1	NY\EfFNHTnWwY4Tpc44hSXO\|YYm=	NUDNNZFIQDRizszN	M3HkclI1KGh?		M3\4TYVvcGGwY3XzJGVITlJicHjvd5Bpd3K7bHH0[YQhdGW4ZXzzMEBidmRidHjlJJBzd3SnaX6gcIV3\Wy\|wrC=	MmK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{e2NFcoRjJ{OUe3OlA4RC:jPh?=
BXPC-3	M3G5VGZ2dmO2aX;uJGF{e2G7	NIjyUJU{QS56NjCg{txO	MkHSNlQhcA>?		Mnza[Y5p[W6lZYOgSWdHWiCyaH;zdIhwenmuYYTl[EBt\X[nbIOsJIFv\CC2aHWgdJJwfGWrbjDs[ZZmdHQEoB?=	M4XUTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUe3OlA4Lz5{Mkm3O\|YxPzxxYU6=
BXPC-3	NWjFOoNXTnWwY4Tpc44hSXO\|YYm=	M2\6ZVM6Njh4IDFOwG0>	M13RfVI1KGh?		MXnpcoR2[2W\|IGOgZZJz\XO2IDjQQFAvODVrIHHu[EBl\WO{ZXHz[ZMhfGinIH71cYJmeiCxZjDj[YxteyCrbjD0bIUhTzJxTTDwbIF{\cLi	NYLZZ2xKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5O\|c3ODdpPkKyPVc4PjB5PD;hQi=>
PANC-1	NWPOb3FXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NGrtRnZKSzVyPUizMlc3yrFyLkG5JO69VQ>?	MnTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{e2NFcoRjJ{OUe3OlA4RC:jPh?=
BXPC-3	Ml;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M2X4d2lEPTB;M{muPFbDuTFwNkig{txO	Mof4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{e2NFcoRjJ{OUe3OlA4RC:jPh?=
Jurkat	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MWK0PEBp		MXTwdo9xd3K2aX;uJI9nKGyrdnWgZ4VtdHN;N{[uO:KyPC5\|IDW=	M32ydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OESwN\|c3Lz5{M{i0NFM4PjxxYU6=
DM-3	NUO0dndyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MV:0PEBp		NGOxWXRxem:yb4L0bY9vKG:oIHzpeoUh[2WubIO9NVAyNjQEsUKuPEAm	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2MEO3Okc,OjN6NECzO\|Y9N2F-
JL-1	M3u1fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MkLUOFghcA>?		NVKxXndoeHKxcH;yeIlwdiCxZjDsbZZmKGOnbHzzQVk5NjMEsUKuNkAm	NUDxRVZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFA{PzZpPkKzPFQxOzd4PD;hQi=>
ZL-34	MnT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NGj6e3c1QCCq		MXTwdo9xd3K2aX;uJI9nKGyrdnWgZ4VtdHN;OUWuO:KyPi53IDW=	NHL2V\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0NFM4Pid-MkO4OFA{PzZ:L3G+
STAV-FCS	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M1XodlQ5KGh?		MXXwdo9xd3K2aX;uJI9nKGyrdnWgZ4VtdHN;OEiuNeKyQC57IDW=	NVPqN2NTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFA{PzZpPkKzPFQxOzd4PD;hQi=>
M-14-K	M135O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MlnWOFghcA>?		MWTwdo9xd3K2aX;uJI9nKGyrdnWgZ4VtdHN;OEGuPOKyOTJwOTCl	MmXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NECzO\|YoRjJ\|OESwN\|c3RC:jPh?=
STAV-AB	NH3jRodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MoP3OFghcA>?		MonDdJJweG:{dHnvckBw\iCuaY\lJINmdGy\|PU[0MlTDuTJzLkigKS=>	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2MEO3Okc,OjN6NECzO\|Y9N2F-
LoVo	MVvGeY5kfGmxbjDBd5NigQ>?	NYDrWYxYOC5yNdMg{txO	MYq3NuKhcA>?	MYjEUXNQyqB?	MlrybY5kemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGy\|IHnuJJRp\SCVIIDoZZNmyqB?	M4[wW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUW5OFYxLz5{M{m1PVQ3ODxxYU6=
HT-29	MXjGeY5kfGmxbjDBd5NigQ>?	NHHTXHExNjB3wrFOwG0>	MXS3NuKhcA>?	NX3iZ4pJTE2VT9Mg	NE\0O2RqdmO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubIOgbY4hfGinIGOgdIhie2YEoB?=	Mm\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NUm0OlAoRjJ\|OUW5OFYxRC:jPh?=
WiDr	M13Yb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NEDnN404OsLiaNMg	NX7xeo5[TE2VT9Mg	Mo\CTWM2OD1yLkCxPUDDuSByLkCwNkDPxE1?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl3OUS2NEc,OjN7NUm0OlA9N2F-
SW1116	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MlrSO\|LDqGkEoB?=	NXnpe5lKTE2VT9Mg	NWTwS25qUUN3ME2xMlcxKMLzIECuNFMh\|ryP	M{fBO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUW5OFYxLz5{M{m1PVQ3ODxxYU6=
HCT116	NYLjUoY5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MYW3NuKhcMLi	MXXEUXNQyqB?	M1nGT2lEPTB;MD6wOFkhyrFiMD6wNVMh\|ryP	MnrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NUm0OlAoRjJ\|OUW5OFYxRC:jPh?=
SW620	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MlPaO\|LDqGkEoB?=	MmDySG1UV8Li	NXi0[oY5UUN3ME2xMlA6KMLzIECuNFEh\|ryP	NVLnd\|RERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OVk1PjBpPkKzPVU6PDZyPD;hQi=>
LoVo	NYHqTpBnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MX23NuKhcMLi	NELuOlVFVVORwrC=	MlzGTWM2OD1yLkCzNkDDuSByLkCwNkDPxE1?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl3OUS2NEc,OjN7NUm0OlA9N2F-
HT-29	M4DaSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NVz4TXRDPzMEoHlCpC=>	NF3EZpBFVVORwrC=	NXXQcWZKUUN3ME2xNE4xPyEEsTCwMlk1KM7:TR?=	NHXSRVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m1PVQ3OCd-MkO5OVk1PjB:L3G+
A549/PEM-16	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MknqPVYhcA>?		M2\uUGlEPTB;NUGuOFUh\|ryP	NX72NFBZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFg5PTRpPkK0N\|Q5QDV2PD;hQi=>
A549/PEM-6.4	M4jadGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NHHmT5U6PiCq		NWnjepZZUUN3ME2yN{4{QSEQvF2=	MlnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NEi4OVQoRjJ2M{S4PFU1RC:jPh?=
A549/PEM-1.6	NF3lNnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MlvHPVYhcA>?		NIr5XXpKSzVyPUWuNFMh\|ryP	MlztQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NEi4OVQoRjJ2M{S4PFU1RC:jPh?=
A549	M1\5Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NY\mUoZGQTZiaB?=		M{TuS2lEPTB;MT6zOUDPxE1?	NGXIOo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0PFg2PCd-MkSzOFg5PTR:L3G+
MSTO-211H	MV;D[YxtKF[rYXLpcIl1gSCDc4PhfS=>	MX2xMVExKM7:Zz;tcC=>	NVrBTY1GPzJiaB?=		M1\BZWlEPTC-MD6wOEDPxGdxbXy=	NXHncGN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzO\|g2PzZpPkK0N\|c5PTd4PD;hQi=>
Y-meso14	NVTRc2M2S2WubDDWbYFjcWyrdImgRZN{[Xl?	M{DFSFEuOTBizsznM41t	NWrjdIZRPzJiaB?=		MkLsTWM2OD5zMDFOwIcwdWx?	NXLmc45ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzO\|g2PzZpPkK0N\|c5PTd4PD;hQi=>
H290	NXXvd\|I{S2WubDDWbYFjcWyrdImgRZN{[Xl?	NUfNXYJKOS1zMDFOwIcwdWx?	NHvwZYY4OiCq		M1T6O2lEPTB-MUCg{txoN22u	NEXoZok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3PFU4Pid-MkSzO\|g2PzZ:L3G+
H226	MXPD[YxtKF[rYXLpcIl1gSCDc4PhfS=>	MYKxMVExKM7:Zz;tcC=>	NF3VSYk4OiCq		NX[xfXNFUUN3ME6xNEDPxGdxbXy=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN5OEW3Okc,OjR\|N{i1O\|Y9N2F-
H1299	NELvSFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1LS[mlEPTB;MT64OEDPxE1?	NGr2Ro09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESxPFUyQSd-MkS0NVg2OTl:L3G+
H1993	M1m5dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1q0WWlEPTB;MD6xO{DPxE1?	MnfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MUi1NVkoRjJ2NEG4OVE6RC:jPh?=
A549	M3zjZmZ2dmO2aX;uJGF{e2G7	M2HMXFAvOS9yLkOvNE42NzFizszN	M2rTbVI1NzR6IHi=	M3HJOGROW09?	MnLKdJJw\HWlZYOgeIhmKG[xcn3heIlwdiCxZjDBWm9{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz	NWLHVnBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlY4OjJpPkK0OlI3PzJ{PD;hQi=>
A549	MkXKR4VtdCCYaXHibYxqfHliQYPzZZk>	Mor5NE4yNzBwMz:wMlUwOSEQvF2=	MXWyOE81QCCq	MmG4SG1UVw>?	NGnLUFVqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfGy7	M{XRVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK2O\|IzLz5{NE[yOlczOjxxYU6=
MES04	NF;RRodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnPuTWM2OO,:nkGwNEDPxE1?	M2DJXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{G0O\|IzLz5{NEexOFczOjxxYU6=
MES01	M3HrdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NYLzVYxiUUN3MP-8olExOCEQvF2=	NX\UV5lyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVQ4OjJpPkK0O\|E1PzJ{PD;hQi=>
H2452	NHj1NVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MkjOTWM2OO,:nkGwNEDPxE1?	M{ftZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{G0O\|IzLz5{NEexOFczOjxxYU6=
H2052	NF\QcZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVfPXZRDUUN3ME2wMlU4yrFyLkO0JO69VQ>?	Mkn3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUS3NlIoRjJ2N{G0O\|IzRC:jPh?=
211H	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MV;JR\|UxRTBwMEhCtVAvODFizszN	NXzER\|BYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVQ4OjJpPkK0O\|E1PzJ{PD;hQi=>
H28	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				Ml24TWM2OD1yLkC3xtExNjB{IN88US=>	Mlq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUS3NlIoRjJ2N{G0O\|IzRC:jPh?=
PC9/GR	M2HuTWZ2dmO2aX;uJGF{e2G7	MkXYOE46PCEQvF2=	MVK3NkBp		NGiyelZqdmO{ZXHz[ZMheC2DS2SgcIV3\Wy\|	M4Xkb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OESwPFkyLz5{NEi0NFg6OTxxYU6=
PC9/GR	MnHtSpVv[3Srb36gRZN{[Xl?	MYC0Mlk1KM7:TR?=	NWG3NXdWPzJiaB?=		M3G1Nolv[3KnYYPld{BxNUWUSzDs[ZZmdHN?	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MEi5NUc,OjR6NEC4PVE9N2F-
PC9/GR	MnnHRZBweHSxc3nzJGF{e2G7	M3LQTlQvQTRizszN	M{n0OVczKGh?		M{\RUYlv\HWlZYOgNVkvPTUxubqgZZBweHSxc3nz	M3T5SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OESwPFkyLz5{NEi0NFg6OTxxYU6=
PC9	Ml:wRZBweHSxc3nzJGF{e2G7	Mn3INVYhdk1?	MYK3NkBp		NXLOO4lMcW6mdXPld{AyPC53NP-5rkBieG:ydH;zbZM>	NHXIXGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0NFg6OSd-MkS4OFA5QTF:L3G+
PC9/GR	M1fPXGZ2dmO2aX;uJGF{e2G7	NW\WSI1GPC57NDFOwG0>	M3\KZVczKGh?		MXXpcoR2[2W\|IGOtdIhie2ViYYLy[ZN1	NXTweXBJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OFA5QTFpPkK0PFQxQDlzPD;hQi=>
PC9	NXPyPYt1TnWwY4Tpc44hSXO\|YYm=	MWqxOkBvVQ>?	Moi3O\|IhcA>?		M33wSYlv\HWlZYOgV{1xcGG\|ZTDhdpJme3R?	Mkm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEC4PVEoRjJ2OESwPFkyRC:jPh?=
PC9/GR	NWjvN5lPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NGrOOWE4OiCq		MWrJR\|UxRTRwOUVCtVAvPDRyIN88US=>	MoPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEC4PVEoRjJ2OESwPFkyRC:jPh?=
PC9	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NVPGO2xEPzJiaB?=		MVnJR\|UxRTF4LkC1xtEyNjh3IH7N	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2MEi5NUc,OjR6NEC4PVE9N2F-
A549	NXPUUo1tTnWwY4Tpc44hSXO\|YYm=	MoXuNE4yNzBwMz:xJO69VQ>?	MnrQOFghcA>?		MV;pcoNz\WG\|ZYOgeIhmKHKjdHnvJI9nKFNvcHjhd4UheG:ydXzheIlwdg>?	M1LHS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OES3PFY{Lz5{NEi0O\|g3OzxxYU6=
A549	MXHBdI9xfG:\|aYOgRZN{[Xl?	MU[wMlEwOC5\|L{Gg{txO	NHfjZnc1QCCq		NEPV[\|NqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=>	MlnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEe4OlMoRjJ2OES3PFY{RC:jPh?=
A549	MWPGeY5kfGmxbjDBd5NigQ>?	NHfQR3YxNjFxMD6zM\|Eh\|ryP	MWW0PEBp		M4Cxb4lv[3KnYYPld{B1cGVibHX2[Ywhd2ZicHjvd5Bpd3K7bHH0[YQhSWu2IHnuJIEh\G:\|ZTDk[ZBmdmSnboSgcYFvdmW{	MkXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEe4OlMoRjJ2OES3PFY{RC:jPh?=
A549	MUjGeY5kfGmxbjDBd5NigQ>?	NWDJeIJFOSEEtV2=	NEnkR3o1NzhxMUKvNlQwPDhiaB?=		NULvUnNZcW6lcnXhd4V{KHSqZTDs[ZZmdCCxZjDwbI9{eGixconsZZRm\CCDa4SgbY4h[SC2aX3lJIRmeGWwZHXueEBu[W6wZYK=	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2N{i2N{c,OjR6NEe4OlM9N2F-
A549	MUjGeY5kfGmxbjDBd5NigQ>?	M4j6flUh\|ryP	M3X2S\|ghcA>?		M3;VUIlv[3KnYYPld{BO[2xvMTD1ZolyfWm2aX7heIlwdiCuZY\lcJM>	NFnsTZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5NVc3QCd-MkS5PVE4Pjh:L3G+
A549	M1Ozb2Fxd3C2b4Ppd{BCe3OjeR?=	MWKyMlUh\|ryP	MVm0PEBp		MVHpcoR2[2W\|IHHwc5B1d3Orcx?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7MUe2PEc,OjR7OUG3Olg9N2F-
H1792	MnPPSpVv[3Srb36gRZN{[Xl?	NVnrZ2t[Oi53L{WvNVAh\|ryP	NYHP[29FPDhiaB?=		MWnkc5dvemWpdXzheIV{KE2lbD2xxsA>	Mon2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUG3OlgoRjJ2OUmxO\|Y5RC:jPh?=
A549	MYfGeY5kfGmxbjDBd5NigQ>?	MYWyMlUwPS9zMDFOwG0>	NXn5Nm1FPDhiaB?=		MUXkc5dvemWpdXzheIV{KE2lbD2xxsA>	NVvoW\|VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVE4PjhpPkK0PVkyPzZ6PD;hQi=>
H1792	MnfTSpVv[3Srb36gRZN{[Xl?	NX\Q[HRMOi53IN88US=>	MmPZOFghcA>?		NX3CbGNwcW6mdXPld{Bk[XOyYYPlMVktKGOjc4Dhd4UuOyCjbnSgdI9tgSBqQVTQMZJq[m:\|ZTmgdI9tgW2ncnHz[UApWEGUUDmgZ4xm[X[jZ3W=	NUK3[FNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5PVE4PjhpPkK0PVkyPzZ6PD;hQi=>
A549	NX7LXpFTTnWwY4Tpc44hSXO\|YYm=	MUWyMlUh\|ryP	MkjlOFghcA>?		MVTpcoR2[2W\|IHPhd5Bie2VvOTygZ4F{eGG\|ZT2zJIFv\CCyb3z5JEhCTFBvcnnic5NmMSCyb3z5cYVz[XOnIDjQRXJRMSClbHXheoFo\Q>?	M1;RcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUmxO\|Y5Lz5{NEm5NVc3QDxxYU6=
H1792	NFXXOGhHfW6ldHnvckBCe3OjeR?=	MW[yMlUwPS9zMDFOwG0>	NIXJSlQ1QCCq		M1rSNolv[3KnYYPld{BPd3ijIHX4dJJme3Orb36gd4lodmmoaXPhcpRtgQ>?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7MUe2PEc,OjR7OUG3Olg9N2F-
A549	MULGeY5kfGmxbjDBd5NigQ>?	MoH2Nk42NzVxMUCg{txO	NY\HdnF[PDhiaB?=		NYfrTI5qcW6lcnXhd4V{KE6xeHGg[ZhxemW\|c3nvckB{cWewaX\pZ4FvfGy7	NGiwUYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm5NVc3QCd-MkS5PVE4Pjh:L3G+
A549	NUHJPYk5TnWwY4Tpc44hSXO\|YYm=	MkPmNUDPxE1?	MVW0PEBp		M{O5boxm[WS\|IITvJI1qfG:laH;u[JJq[WxiZInz[pVv[3Srb36gZ49u[mmwZXSge4l1cCC\|aX32ZZN1[XSrbh?=	NXHRSYliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPVY6QTNpPkK1NFk3QTl\|PD;hQi=>
MSTO-211	MX3GeY5kfGmxbjDBd5NigQ>?	M2TL[\|Eh\|ryP	M4nIN\|Q5KGh?		MlOxcIVi\HNidH:gcYl1d2Oqb37kdolidCCmeYPmeY5kfGmxbjDjc41jcW6nZDD3bZRpKHOrbY\hd5RifGmw	NVv6cYQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPVY6QTNpPkK1NFk3QTl\|PD;hQi=>
A549	NHTzTm1HfW6ldHnvckBCe3OjeR?=	MlLkNUDPxE1?	NXTCWpZPPDhiaB?=		MmrI[Y5p[W6lZYOgbY51emGlZXzseYxieiCUT2OgdJJw\HWldHnvckBkd22kaX7l[EB4cXSqIIPpcZZie3SjdHnu	NHjJTJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC5Olk6Oyd-MkWwPVY6QTN:L3G+
MSTO-211	NYnLXmRqTnWwY4Tpc44hSXO\|YYm=	M1XvZlEh\|ryP	NXzCdW1XPDhiaB?=		M{HBfoVvcGGwY3XzJIlvfHKjY3XscJVt[XJiUl;TJJBzd2S3Y4Tpc44h[2:vYnnu[YQhf2m2aDDzbY13[XO2YYTpci=>	NUTrfZo6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPVY6QTNpPkK1NFk3QTl\|PD;hQi=>
A549	MknQRZBweHSxc3nzJGF{e2G7	MV2xJO69VQ>?	NUD2Oos1PDhiaB?=		NXjDV3FF\W6qYX7j[ZMh[2G\|cHHz[U1l\XCnbnTlcpQh[XCxcITvd4l{KGOxbXLpcoVlKHerdHigd4lufmG\|dHH0bY4>	NGG3V4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC5Olk6Oyd-MkWwPVY6QTN:L3G+
MSTO-211	MkDKRZBweHSxc3nzJGF{e2G7	MUCxJO69VQ>?	M1nPXFQ5KGh?		NVW5bGFC\W6qYX7j[ZMh[2G\|cHHz[U1l\XCnbnTlcpQh[XCxcITvd4l{KGOxbXLpcoVlKHerdHigd4lufmG\|dHH0bY4>	MknGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOU[5PVMoRjJ3MEm2PVk{RC:jPh?=
A549	M4LPV2NmdGxiVnnhZoltcXS7IFHzd4F6	MlfxNUDPxE1?	NGnJUms1QCCq		NFTPW2xxem:mdXPld{BiKHO7bnXy[4l{fGmlIHnubIljcXSxcomg[YZn\WO2IH;uJJRp\SClZXzsJIdzd3e2aDDjc41jcW6nZDD3bZRpKHOrbY\hd5RifGmw	NUjCOXNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwPVY6QTNpPkK1NFk3QTl\|PD;hQi=>
MSTO-211	Mnf6R4VtdCCYaXHibYxqfHliQYPzZZk>	NV\ZblBPOSEQvF2=	NXHqWpN1PDhiaB?=		NVTy[Fk2eHKxZIXj[ZMh[SC\|eX7ldodqe3SrYzDpcohq[mm2b4L5JIVn\mWldDDvckB1cGViY3XscEBoem:5dHigZ49u[mmwZXSge4l1cCC\|aX32ZZN1[XSrbh?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB7Nkm5N{c,OjVyOU[5PVM9N2F-
H1299	MmXLR4VtdCCYaXHibYxqfHliQYPzZZk>	MonjNU0yODByIH7N	M4jUS\|czKGh?		MULJR\|UxRTF5ODDuUS=>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF2NU[2PUc,OjVzNEW2Olk9N2F-
A549	NWLobnE{S2WubDDWbYFjcWyrdImgRZN{[Xl?	NH3ueVcyNTFyMECgcm0>	MUS3NkBp		M2T4ZmlEPTB;MUO3JI5O	Mk\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNEW2OlkoRjJ3MUS1OlY6RC:jPh?=
MG-63	NH\JRXVE\WyuIG\pZYJqdGm2eTDBd5NigQ>?	MYewMlAyNTFyMDFOwG0>	NEfyb5E4OiCq		MXvpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4Pl[IVx\W6mZX70cJk>	NEHZVII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG1NlM6QSd-MkWxOVI{QTl:L3G+
U20S	Mo\SR4VtdCCYaXHibYxqfHliQYPzZZk>	M4LxT\|AvODFvMUCwJO69VQ>?	NIDDSnQ4OiCq		M4jMR4lvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Vl\XCnbnTlcpRtgQ>?	NICyc5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG1NlM6QSd-MkWxOVI{QTl:L3G+
HepG3	MYTGeY5kfGmxbjDBd5NigQ>?	NIrWcZcyOMLizszN	MmPkOFghcA>?		M1;jZ5VxemWpdXzheIV{KHCqb4PwbI9zgWyjdHXkJEhxNSliTVXLNU8zKCiVZYKyNVcwOjJzKTDhcoQheC2HUluxM\|IhMFSqckKwNk9VgXJ{MESp	MnHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NE[xNFIoRjJ3NES2NVAzRC:jPh?=
HepG3	MmjzSpVv[3Srb36gRZN{[Xl?	NUnXTnEyOC5z4pETNVAxyqEQvF2=	MWC0PEBp		NXzSc5F7[WO2aY\heIV{KGO7dH:tdJJwfGWldHn2[UBifXSxcHjh[5nDqA>?	NX[0SohyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFYyODJpPkK1OFQ3OTB{PD;hQi=>
HepG3	MlfXSpVv[3Srb36gRZN{[Xl?	Mke0NE4y6oDVMUCwxsDPxE1?	MV20PEBp		NH3DU2NqdmS3Y3XzJJA3OiCmb4fudoVofWyjdHnvckBieyC5ZXzsJIF{KEKnY3zpck0yKGGwZDDMR\|NDNUmLIIXwdoVofWyjdHnvci=>	NY\nRopZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFYyODJpPkK1OFQ3OTB{PD;hQi=>
HepG2	MnWwRZBweHSxc3nzJGF{e2G7	NFLCboExNjIkgKOxNFDDqM7:TR?=	Mn\DO\|IhcA>?		NVf6e45ncW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJkh[XRiaHnnbEBkd26lZYLueJJifGmxbh?=	NG\ZSY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS0OlExOid-MkW0OFYyODJ:L3G+
HepG2	NX74NWw6S2WubDDWbYFjcWyrdImgRZN{[Xl?	MnfFNE4y6oDVMUCwxsDPxE1?	M1nPXlczKGh?		NWjWNmlRcGmpaDDjc45k\W62cnH0bY9veyCxZjDw[Y1mfHKneHXkJIF1KChzMD:xNFDDqM7:TTmgc45tgSC\|bHnnbJRtgSCrbnjpZol1eyCKZYDHNkBk\WyuIIP1dpZqfmGu	MoDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NE[xNFIoRjJ3NES2NVAzRC:jPh?=
A459	NFXTUWxCeG:ydH;zbZMhSXO\|YYm=	NFf2b4Q1\|ryP	MlzIOFghcA>?		MWXpcoR2[2W\|IHHwc5B1d3Orcx?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd2M{iyNkc,OjV5NEO4NlI9N2F-
A459	M17rS2Z2dmO2aX;uJGF{e2G7	NWD0PHloOS9{L{Sg{txO	MnvuOFghcA>?		MVzk[YNz\WG\|ZYOgeIhmKGyndnXsd{Bw\iCyLVHreC=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd2M{iyNkc,OjV5NEO4NlI9N2F-
A459	NGX3Z\|hHfW6ldHnvckBCe3OjeR?=	MYSxM\|IwPCEQvF2=	Mlz3NlQwPDhiaB?=		MnGwbY5lfWOnczDHNUBxcGG\|ZTDhdpJme3RiaX6g[I9{\S1iYX7kJJRqdWViZHXw[Y5l\W62IH3hco5meg>?	MlnIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NEO4NlIoRjJ3N{SzPFIzRC:jPh?=
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	p-Chk1 / Chk1 / Cyclin D / Cyclin E / p-Histone H3 / Histone H3 / Cyclin B1 / p-Cdc2 / Cdc2 ; Topo IIα / Topo I / γH2AX / Cleaved PARP / Survivin ; AKT / p-AKT / GSK3β / p-GSK3β ; EGFR / p-EGFR	22237209 24847863 30953548
Immunofluorescence	p-AKT	24847863
Growth inhibition assay	Cell viability	28719077

In vivo

In the human H460 non-small cell lung carcinoma xenograft, Pemetrexed disodium produces a duration-dependent tumor growth delay (TGD). ^[3]

Protocol (from reference)

Kinase Assay:^[1]	Enzyme Assays and Methods. : TS activity is assayed using a spectrophotometric method, which involved monitoring the increase in absorbance at 340 nm resulting from formation of the product, 7,8-dihydrofolate. The assay buffer contains 50 mM N-tris[hydroxymethyljmethyl-2-aminoethanesulfonic acid, 25 mM MgC1₂, 6.5 mM formaldehyde, 1 mM EDTA, and 75 mM 2-mercaptoethanol, pH 7.4. The concentrations of deoxyuridylate monophosphate, 6R-MTHF, and hIS are 100 μM, 30μM and 30 nM (1.7 milliunits/mL), respectively. At the 6R-MTHF concentration, an uninhibited reaction and six concentrations of inhibitor are assayed. K_{i app} values are determined by fitting the data to the Morrison equation using nonlinear regression analysis with the aid of the program ENZFITTER. K_i values are calculated using the equation: K_{i app}= K_i(1 + [S]/K_m), where [S] is equal to 30 μM and K_m is equal to 3 μM. DHFR activity is assayed spectrophotometrically by monitoring the dis appearance of the substrates NADPH and 7,8-dihydrofolate at 340 nm. The reaction takes place at 25°C in 0.5 mL of 50 mM potassium phosphate buffer, which contains 150 mM KC1 and 10 nM 2-mercaptoethanol, pH 7.5, and 14 nM (0.34 milliunitlmL) DHFR. The NADPH concentration is 10 μM and 7,8-dihydrofolate is varied at 5, 10, or 15 μM. At each 7,8-dihydrofolate concentration, an uninhibited reaction and seven concentrations of inhibitor are assayed. The ENZFITI'ER microcomputer program is used to obtain K_{i app} values by fitting the data to the Morrison equation by nonlinear regression analysis. K_{i app}= K_i(1 + [S]/K_m), where [S] is equal to the concentration of 7,8-dihydrofolate used and K_m of 7,8-dihydrofolate is equal to 0.15 μM. GARFT activity is assayed spectrophotometrically by monitoring the increase of absorbance resulting from formation of the product 5,8-dideazafolate at 295 nm. The reaction solvent contains 75 mM HEPES, 20% glycerol, and 50 mM a-thioglygerol, pH 7.5, at 25°C. The concentrations of substrates and enzyme used are 10 μM α,β-glycinamide ribonucleotide, 0-10 μM 10-formyl-5,8-dideazafolic acid, and 10 nM (1.9 milliunits/mL) GARFT. K_i values are calculated using the Enzyme Mechanism program of the Beckman DU640 spectrophotometer, which uses nonlinear regression analysis to fit data to the Michaelis-Menten equation for competitive inhibition.
Cell Research:^[1]	Cell lines: CCRF-CEM leukemia, GC3/C1 colon carcinoma, and HCT-8 ileocecal carcinoma cells. Concentrations: 0-30 μM Incubation Time: 72 hours Method: Dose-response curves are generated to determine the concentration required for 50% inhibition of growth (IC50). Pemetrexed disodium is dissolved initially in DMSO at a concentration of 4 mg/mL and further diluted with cell culture medium to the desired concentration. CCRF-CEM leukemia cells in complete medium are added to 24-well Cluster plates in a total volume of 2.0 mL. Pemetrexed disodium at various concentrations are added to duplicate wells so that the final volume of DMSO is 0.5%. The plates are incubated for 72 hour at 37 °C in an atmosphere of 5% CO₂ in air. At the end of the incubation, cell numbers are determined on a ZBI Coulter counter. For several studies, IC50s are determined for each compound in the presence of either 300 μM AICA, 5 μM thymidine, 100 μM hypoxanthine, or combination of 5 μM hymidine plus 100 μM hypoxanthine. For adherent tumor cells, a modification of the original MTT colorimetric assay is used to measure cell cytotoxicity. The human tumor cells are seeded in 100 μL assay medium/well in 96-well flat-bottomed tissue culture plates. The assay medium contains folic acid-free RPMI 1640 supplemented with 10% FCS and either 2 nM folinic acid or 2.3 μM folic acid as the sole folate source. Well 1A is left blank. Stock solutions of antifolates are prepared in Dulbecco's PBS at 1 mg/mL, and a series of 2-fold dilutions are subsequently made in PBS. Ten-μL aliquots of each concentration are added to triplicate wells. Plates are incubated for 72 hours at 37 °C in a humidified atmosphere of 5% CO₂-in-air. MTT is dissolved in PBS at 5 mg/mL, 10 μL of stock MTF solution are added to each well of an assay, and the plates are incubated at 37 °C for 2 additional hours. Following incubation, 100 μL of DMSO are added to each well. After thorough formazan solubilization, the plates are read on a Dynatech MR600 reader, using a test wavelength of 570 nm and a reference wavelength of 630 nm. The IC50 is determined as the concentration of drug required to inhibit cell growth by 50% compared to an untreated controls.
Animal Research:^[3]	Animal Models: EMT-6 mammary carcinoma, the human HCT 116 colon carcinoma, and the human H460 non-small cell lung carcinoma are injected s.c. into the nude mice. Dosages: 100 mg/kg or 150 mg/kg Administration: Administered via i.p.

References

Solubility (25°C)

In vitro	Water	94 mg/mL (199.41 mM)
	DMSO	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): Saline For best results, use promptly after mixing.	30 mg/mL

Chemical Information

Molecular Weight	471.37
Formula	C₂₀H₁₉N₅Na₂O₆
CAS No.	150399-23-8
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC(=CC=C1CCC2=CNC3=C2C(=O)NC(=N3)N)C(=O)NC(CCC(=O)[O-])C(=O)[O-].[Na+].[Na+]

Download Pemetrexed (LY-231514) disodium SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04173338	Recruiting	Drug: Cabozantinib\|Drug: Pemetrexed	Non Small Cell Lung Cancer\|Non-squamous Non-small-cell Lung Cancer\|Urothelial Carcinoma\|Malignant Mesothelioma	Augusta University	January 23 2020	Phase 1
NCT03023319	Recruiting	Drug: Bosutinib\|Drug: Pemetrexed	Carcinoma Non-Small-Cell Lung\|Mesothelioma\|Bladder Cancer\|Ovarian Cancer\|Peritoneal Cancer\|Thymoma\|Thymus Cancer\|Uterine Cervical Cancer	Nagla Abdel Karim\|Augusta University	December 10 2019	Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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