Nutlin-3

Catalog No.S1061

For research use only.

Nutlin-3 is a potent and selective Mdm2 (RING finger-dependent ubiquitin protein ligase for itself and p53) antagonist with IC50 of 90 nM in a cell-free assay; stabilizes p73 in p53-deficient cells.

Nutlin-3 Chemical Structure

CAS No. 890090-75-2

Selleck's Nutlin-3 has been cited by 80 publications

Purity & Quality Control

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Biological Activity

Description Nutlin-3 is a potent and selective Mdm2 (RING finger-dependent ubiquitin protein ligase for itself and p53) antagonist with IC50 of 90 nM in a cell-free assay; stabilizes p73 in p53-deficient cells.
Targets
MDM2 [5]
(Cell-free assay)
180 nM
In vitro

Nutlin-3 potently inhibits the MDM2-p53 interaction, leading to the activation of the p53 pathway. Nutlin-3 treatment induces the expression of MDM2 and p21, and displays potent antiproliferative activity with IC50 of ~1.5 μM, only in cells with wild-type p53 such as HCT116, RKO and SJSA-1, but not in the mutant p53 cell lines SW480 and MDA-MB-435. In SJSA-1 cells, Nutlin-3 treatment at 10 μM for 48 hours significantly induces caspase-dependent cell apoptosis by ~45%. Although Nutlin-3 also inhibits the growth and viability of human skin (1043SK) and mouse embryo (NIH/3T3) with IC50 of 2.2 μM and 1.3 μM, respectively, cells remain viable 1 week post-treatment even at 10 μM of Nutlin-3, in contrast with the SJSA-1 cells with viability lost at 3 μM of Nutlin-3 treatment. [1] Nutlin-3 does not induce the phosphorylation of p53 on key serine residues and reveals no difference in their sequence-specific DNA binding and ability to transactivate p53 target genes compared with phosphorylated p53 induced by the genotoxic drugs doxorubicin and etoposide, demonstrating that phosphorylation of p53 on key serines is dispensable for transcriptional activation and apoptosis. [2] Although binding less efficiently to MDMX than to MDM2, Nutlin-3 can block the MDMX–p53 interaction and induce the p53 pathway in retinoblastoma cells (Weri1) with IC50 of 0.7 μM. [3] Nutlin-3 at 30 μM also disrupts endogenous p73-HDM2 interaction and enhances the stability and proapoptotic activities of p73, leading to the dose-dependent cell growth inhibition and apoptosis induction in cells without wild-type p53. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SMMC-7721  NWrWdFBVTnWwY4Tpc44hSXO|YYm= MWexNEDPxE1? M2jqXFM3KGh? MXrEUXNQ M1;veYRwf25vcnXneYxifGW|IITo[UBxem:2ZXnuJIV5eHKnc4Ppc44hdGW4ZXygc4YheGixc4Doc{1U\XJ|OUKtdFU{ NEXiVpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK4OlMyOid-MkSyPFY{OTJ:L3G+
HuH-7 M3TR[2Fxd3C2b4Ppd{BCe3OjeR?= NXrRc2o2OjBizszN NF7lbIQ1QCCq NELxdo5FVVOR MmjDbY5lfWOnczDhdI9xfG:|aYO= M3zlZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mki2N|EzLz5{NEK4OlMyOjxxYU6=
SMMC-7721  NWfaeGljSXCxcITvd4l{KEG|c3H5 M3XrSFIxKM7:TR?= NVrqb|NxPDhiaB?= MWDEUXNQ NUm3cWRxcW6mdXPld{BieG:ydH;zbZM> NXzieo94RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPFY{OTJpPkK0Nlg3OzF{PD;hQi=>
HuH-7 NU\XfVdLS2WubDDWbYFjcWyrdImgRZN{[Xl? MkXvNU4zPS1{MDFOwG0> MYqyOE81QC95MjDo MX7EUXNQ NYW1TVhVcW6qaXLpeJMh[2WubDDwdo9tcW[ncnH0bY9vKGSxc3WgZY5lKHSrbXWg[IVx\W6mZX70cJk> MmL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{OE[zNVIoRjJ2Mki2N|EzRC:jPh?=
SMMC-7721  M{Xi[GNmdGxiVnnhZoltcXS7IFHzd4F6 M1fOS|EvOjVvMkCg{txO MYmyOE81QC95MjDo NFjKPFJFVVOR NITYRmtqdmirYnn0d{Bk\WyuIIDyc4xq\mW{YYTpc44h\G:|ZTDhcoQhfGmvZTDk[ZBmdmSnboTsfS=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ6NkOxNkc,OjR{OE[zNVI9N2F-
RKO M1[1OmZ2dmO2aX;uJGF{e2G7 M1LHd|IxKM7:TR?= MYCyOEBp MWDpcoR2[2W|IFjPMVEh\XiycnXzd4lwdiCjdDD0bIUhdGW4ZXygc4YhfHKjboPjdolxfGmxbh?= Mnz1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|Nk[wNFcoRjJ2M{[2NFA4RC:jPh?=
U2OS  MnK3SpVv[3Srb36gRZN{[Xl? M{S1PVIxKM7:TR?= MkHTNlQhcA>? M3LOVYlv\HWlZYOgTG8uOSCneIDy[ZN{cW:wIHH0JJRp\SCuZY\lcEBw\iC2cnHud4NzcXC2aX;u NH7ZXmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2OlAxPyd-MkSzOlYxODd:L3G+
RKO M37DeWZ2dmO2aX;uJGF{e2G7 MVOyNEDPxE1? NWj1U497OjRiaB?= MYrpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC2aHWgTG8uOSCycn;0[YlvKGG|IIflcIwh[XNidHjlJJA2OyCycn;0[Ylv MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4NkCwO{c,OjR|Nk[wNFc9N2F-
U2OS  M1:zbGZ2dmO2aX;uJGF{e2G7 MX:yNEDPxE1? M3r0SlI1KGh? MVPpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC2aHWgTG8uOSCycn;0[YlvKGG|IIflcIwh[XNidHjlJJA2OyCycn;0[Ylv MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4NkCwO{c,OjR|Nk[wNFc9N2F-
MOLM MkfoSpVv[3Srb36gRZN{[Xl? MYCxNEDPxE1? NGjPdoQzPCCq NYTqWVN5fXC{ZXf1cIF1\XNidHjlJHNQS1NvMTDlfJBz\XO|aX;u M{TsUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEezOVYzLz5{NES3N|U3OjxxYU6=
OCI MU\GeY5kfGmxbjDBd5NigQ>? NE\MRpQyOCEQvF2= M1nhblI1KGh? MU\1dJJm\3WuYYTld{B1cGViU1;DV{0yKGW6cILld5Nqd25? M2Ty[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEezOVYzLz5{NES3N|U3OjxxYU6=
BeWo M3fmS2Fxd3C2b4Ppd{BCe3OjeR?= NIXMRmk{OCEEtV2= MofNNlQhcA>? NUf3[WFucW6lcnXhd4V{KGGyb4D0c5Nqew>? NF3LcYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5PFE2PCd-MkS0PVgyPTR:L3G+
BeWo MoXaSpVv[3Srb36gRZN{[Xl? MnjjN|AhyrWP MXKyOEBp M3XPVYlv[3KnYYPld{BxPTNuIF3kcVItKHB{MTDhcoQhWHWvYTDheEB1cGVicILveIVqdiCuZY\lcC=> NHPYdoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5PFE2PCd-MkS0PVgyPTR:L3G+
MOML13 NXLIN3ZOTnWwY4Tpc44hSXO|YYm= MlzoNVDPxE1? MoP2Nk81KGh? MlPFbY5kemWjc3XzJJRp\SCuZY\lcEBw\iCyNUO= M{joZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkW5O|Q6Lz5{NE[1PVc1QTxxYU6=
AML3 M2T0TmZ2dmO2aX;uJGF{e2G7 MWWxNO69VQ>? M2rvcVIwPCCq Mn71bY5kemWjc3XzJJRp\SCuZY\lcEBw\iCyNUO= NEj3c5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[1PVc1QSd-MkS2OVk4PDl:L3G+
AML2 MojKSpVv[3Srb36gRZN{[Xl? Mnu1NVDPxE1? M1\mdlIwPCCq NHr4VXVqdmO{ZXHz[ZMhfGinIHzleoVtKG:oIIC1Ny=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ3OUe0PUc,OjR4NUm3OFk9N2F-
MOML13 NFu4NYVCeG:ydH;zbZMhSXO|YYm= M3nVRlIwOTBizszN NFTzUIQzPC92ODDo MnXIbY5lfWOnczDhdI9xfG:|aYO= M3SxTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkW5O|Q6Lz5{NE[1PVc1QTxxYU6=
AML2 NYHkOHExSXCxcITvd4l{KEG|c3H5 NViwWY1mOi9zMDFOwG0> NGntRngzPC92ODDo NWXWd5ZRcW6mdXPld{BieG:ydH;zbZM> NETV[VQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[1PVc1QSd-MkS2OVk4PDl:L3G+
U2OS  NYXyflBLTnWwY4Tpc44hSXO|YYm= NFW1UVMzOCEQvF2= NYHRb|AyOjRiaB?= MXzpcoNz\WG|ZYOgeIhmKG2UTlGgcIV3\Wy|IH;mxsBDS0x{QUGsJGJEVFiOwrDhcoRDS0yZ MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh4N{K1PUc,OjR6NkeyOVk9N2F-
Hep3B MWHBdI9xfG:|aYOgRZN{[Xl? NX\xbZNIcW6mdXPld{BieG:ydH;zbZM> Mk\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OES4NFkoRjJ2OEi0PFA6RC:jPh?=
Huh-7 NYnIc|NVSXCxcITvd4l{KEG|c3H5 MkfTbY5lfWOnczDhdI9xfG:|aYO= Mnm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OES4NFkoRjJ2OEi0PFA6RC:jPh?=
SMMC7721 M1jaPWFxd3C2b4Ppd{BCe3OjeR?= MYTpcoR2[2W|IHHwc5B1d3Orcx?= NG\kbY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi4OFgxQSd-MkS4PFQ5ODl:L3G+
HepG2 NGTCXGRCeG:ydH;zbZMhSXO|YYm= NWLYXZk2cW6mdXPld{BieG:ydH;zbZM> NWWyeIlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4PFQ5ODlpPkK0PFg1QDB7PD;hQi=>
Hep3B NYnNRpBST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXZO|IhcA>? M{fpV2ROW09? MnvnTWM2OD1{MD6xPEDDuSBzLki0JO69VQ>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh6NEiwPUc,OjR6OES4NFk9N2F-
Huh-7 NHjFVVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPMfJltPzJiaB?= NYXScWhZTE2VTx?= M{\yfGlEPTB;M{OuPVYhyrFiMz65JO69VQ>? NHjERoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi4OFgxQSd-MkS4PFQ5ODl:L3G+
SMMC7721/Ac MnG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrETXJUPzJiaB?= MWfEUXNQ MlmxTWM2OD13NT6yNUDDuSB3LkCzJO69VQ>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh6NEiwPUc,OjR6OES4NFk9N2F-
SMMC7721 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;QWVczKGh? NED4V4RFVVOR NF[yeXNKSzVyPUOxMlI5KMLzIESuNkDPxE1? M4n0ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEi0PFA6Lz5{NEi4OFgxQTxxYU6=
HepG2/As M{PCNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rlWlczKGh? NWm2VmpETE2VTx?= MYjJR|UxRTZ6LkGzJOKyKDlwNjFOwG0> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh6NEiwPUc,OjR6OES4NFk9N2F-
HepG2 MmG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rsTVczKGh? NUizcJJpTE2VTx?= NH;xbZRKSzVyPUO1Mlg3KMLzIEKuPUDPxE1? Ml:2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OES4NFkoRjJ2OEi0PFA6RC:jPh?=
MOLM-13 Mmm0SpVv[3Srb36gRZN{[Xl? M3n3OVbDqM7:TR?= M2TBRVYhcA>? NYXwOVd1TE2VTx?= MoG4[Y5p[W6lZYOgeIhmKGGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFJDKGGwZDDo[YF1KHOqb3PrJJBzd3SnaX7zJGh{eDJ5IHHu[EBJe3B7MB?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh6NUC4Nkc,OjR6OEWwPFI9N2F-
MOLM-13 MYXGeY5kfGmxbjDBd5NigQ>? M3\6TlbDqM7:TR?= MmrtNE05KGh? NVz2R|dUTE2VTx?= M1y3eYlv[3KnYYPld{B1cGVibHX2[Yx{KG:oIIC1N{whVUSPMjygdFIyKGGwZDDhZ4V1gWyjdHXkJJA2Ow>? MmXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OEWwPFIoRjJ2OEi1NFgzRC:jPh?=
115 M4\tXGZ2dmO2aX;uJGF{e2G7 M3iz[FUh|ryP NVzqe29{PDhiaB?= MVLEUXNQ NHXIV2RqdmS3Y3XzJJA2Oy2mZYDlcoRmdnRic3Xu[ZNk\W6lZdMg NVPVcVM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlc4QDdpPkK1NFY4Pzh5PD;hQi=>
A498 NYjReWs{TnWwY4Tpc44hSXO|YYm= NVOxdopnPSEQvF2= M2TKWlQ5KGh? NYfhb|lvTE2VTx?= NXK2RoxmcW6mdXPld{BxPTNvZHXw[Y5l\W62IIPlcoV{[2WwY3ZCpC=> NFLtcWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2O|c5Pyd-MkWwOlc4QDd:L3G+
Caki-2 NX[2dHBTTnWwY4Tpc44hSXO|YYm= MlnUOUDPxE1? NGDaeJk1QCCq NU[3dodiTE2VTx?= MVTpcoR2[2W|IIC1N{1l\XCnbnTlcpQhe2WwZYPj[Y5k\cLi NGfOd3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2O|c5Pyd-MkWwOlc4QDd:L3G+
ACHN MnXpSpVv[3Srb36gRZN{[Xl? MonzOUDPxE1? MlHnOFghcA>? MofQSG1UVw>? NInYVFFqdmS3Y3XzJJA2Oy2mZYDlcoRmdnRic3Xu[ZNk\W6lZdMg MmWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNke3PFcoRjJ3ME[3O|g4RC:jPh?=
115 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUS1JO69VQ>? MYS0PEBp Mn;MSG1UVw>? MlLEbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dNMg MkiyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNke3PFcoRjJ3ME[3O|g4RC:jPh?=
A498 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\sOUDPxE1? M1TPVlQ5KGh? MkG5SG1UVw>? MlPxbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dNMg NHvEZ3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2O|c5Pyd-MkWwOlc4QDd:L3G+
Caki-2 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXMOUDPxE1? M2XLUlQ5KGh? MnThSG1UVw>? MmjVbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dNMg NE\VdJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2O|c5Pyd-MkWwOlc4QDd:L3G+
ACHN M1;Lemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1OzNlUh|ryP NVzSSpJuPDhiaB?= NVTWUlhXTE2VTx?= MnvMbY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dNMg MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4N{e4O{c,OjVyNke3PFc9N2F-
115 M3LwcGZ2dmO2aX;uJGF{e2G7 MVuwMlUwOS93IN88US=> MYi0PEBp NFWzT3VFVVOR Mo\rcIVi\HNidH:gbY5kemWjc3XkJIV5eHKnc4Ppc44hd2ZicEWzJIFv\CC|b33lJJA2OyC2YYLn[ZQh\2WwZYO6JG1FVTJuIHHu[EBxOjF? M1roUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[3O|g4Lz5{NUC2O|c5PzxxYU6=
A498 NHrSNoRHfW6ldHnvckBCe3OjeR?= NWrn[lhlOC53L{GvOUDPxE1? NWXyPXJmPDhiaB?= MVLEUXNQ MlXucIVi\HNidH:gbY5kemWjc3XkJIV5eHKnc4Ppc44hd2ZicEWzJIFv\CC|b33lJJA2OyC2YYLn[ZQh\2WwZYO6JG1FVTJuIHHu[EBxOjF? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4N{e4O{c,OjVyNke3PFc9N2F-
Caki-2 NFz1TZhHfW6ldHnvckBCe3OjeR?= MXiwMlUwOS93IN88US=> MXi0PEBp M3nM[mROW09? Ml\NcIVi\HNidH:gbY5kemWjc3XkJIV5eHKnc4Ppc44hd2ZicEWzJIFv\CC|b33lJJA2OyC2YYLn[ZQh\2WwZYO6JG1FVTJuIHHu[EBxOjF? MnvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNke3PFcoRjJ3ME[3O|g4RC:jPh?=
ACHN MnfvSpVv[3Srb36gRZN{[Xl? Mo\MNE42NzFxNTFOwG0> MXW0PEBp MULEUXNQ NHXr[Ylt\WGmczD0c{BqdmO{ZXHz[YQh\XiycnXzd4lwdiCxZjDwOVMh[W6mIIPvcYUheDV|IIThdodmfCCpZX7ld|ohVUSPMjygZY5lKHB{MR?= NYf1VXVsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlc4QDdpPkK1NFY4Pzh5PD;hQi=>
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Caki-2 NF3h[YVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NV\6XVZZOC53LUGwJO69VQ>? M3fvcFAuPiCm NXPafWd[TE2VTx?= MkHnbY5pcWKrdIOgdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M{njd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[3O|g4Lz5{NUC2O|c5PzxxYU6=
ACHN MVLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MXmwMlUuOTBizszN MkT0NE03KGR? MlLvSG1UVw>? NVn2UWRtcW6qaXLpeJMheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NH21Tno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2O|c5Pyd-MkWwOlc4QDd:L3G+
MCF7  NV[xNIpJTnWwY4Tpc44hSXO|YYm= MlfrNk42KML3TR?= MlPROFghcA>? NY\kVIN7TE2VTx?= Mlnj[IVkemWjc3XzJJRp\SCqb33vcI9od3W|IFTTRkBz\XCjaYKg[pJmeXWnbnPp[ZM> MojnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOEW5NFIoRjJ3MEi1PVAzRC:jPh?=
MCF7  NGfBXJZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYLLd|ZtOi53INM1US=> NHLmTYM2KGR? NHjwOGZFVVOR NXnRe|NHe2Wwc3n0bZpmeyCPQ1[3JJRwKFCDUmCgbY5pcWKrdHnvci=> Mnv2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOEW5NFIoRjJ3MEi1PVAzRC:jPh?=
RAW 264.7 M2\3bWZ2dmO2aX;uJGF{e2G7 MUCxNOKh|ryP Ml\XN|AhdWmw NGfUdmlqdmirYnn0d{BNWFNvaX7keYNm\CCQTzDwdo9lfWO2aX;uxsA> M2jSN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUeyOVQ4Lz5{NUG3NlU1PzxxYU6=
RAW 264.7 NH2xcZlHfW6ldHnvckBCe3OjeR?= NV:1bpFnOTEEoN88US=> NGHpRYI{OCCvaX6= MYHy[YR2[2W|IITo[UBNWFNvYYXncYVvfGWmIITo[UBPTi4QulKgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHjeIl3cXS7 NVv0WI9LRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxO|I2PDdpPkK1NVczPTR5PD;hQi=>
RAW 264.7 M4rib2Z2dmO2aX;uJGF{e2G7 M2rZU|ExyqEQvF2= M3;CcVMxKG2rbh?= MlTsdJJmfmWwdIOgeIhmKHB3MzDy[YR2[3Srb36gbY4hemW|cH;ud4UhfG9iTGDT MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF5MkW0O{c,OjVzN{K1OFc9N2F-
SUM102PT MUjGeY5kfGmxbjDBd5NigQ>? MYWxNOKh|ryP M1fqWVI1KGh? NHW4[nZFVVOR MYrpcohq[mm2czCgWGdHNc7{Mz3pcoR2[2WmIFXQTGIzyqCvUl7BJIFv\CCycn;0[YlvKGW6cILld5Nqd25? MnvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NUe3NlkoRjJ3MkW3O|I6RC:jPh?=
SK-BR-7 M3G0S2Z2dmO2aX;uJGF{e2G7 M{i2dFExyqEQvF2= Mke2NlQhcA>? MoPFSG1UVw>? NX3QN|F[cW6qaXLpeJMhKFSJRj5OtlMucW6mdXPl[EBGWEiEMtMgcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO|aX;u MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ3N{eyPUc,OjV{NUe3Nlk9N2F-
MCF-10CA1a NYXNOIs6TnWwY4Tpc44hSXO|YYm= MVuxNOKh|ryP M{DqcVI1KGh? NH3aVWtFVVOR NGrNWnFqdmirYnn0d{AhXEeILd8yN{1qdmS3Y3XkJGVRUEJ{wrDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36= NUTiZWZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOVc4OjlpPkK1NlU4PzJ7PD;hQi=>
MCF-10CA1a MlXESpVv[3Srb36gRZN{[Xl? MX2xNOKh|ryP M{POSFI1KGh? NEmxfFRFVVOR NYjkVHJL\GWlcnXhd4V{KHSqZTDUS2Yu|rJ|LXnu[JVk\WRibWLORUBt\X[nbIOgc4ZOVVB{LNMgUW1RQSxiYX7kxsBqdnSnZ4LpcuKh|rMEoEO= M{nvTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkW3O|I6Lz5{NUK1O|czQTxxYU6=
MCF-10A1  NXq3WWJmTnWwY4Tpc44hSXO|YYm= NYTKOoFnOTEEoN88US=> NV3Ed|B5OjRxNEigbC=> NEPz[nhFVVOR MlzvbY5pcWKrdIOgcYloemG2aX;uJI9nKG6xcn3hcEBjemWjc4Sg[ZBqfGinbHnhcOKh NYr4S2J7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyOVc4OjlpPkK1NlU4PzJ7PD;hQi=>
MCF-10CA1a MUXGeY5kfGmxbjDBd5NigQ>? M4LKRlExyqEQvF2= MlHqOFghcA>? M3HFRmROW09? NFXDb|BqdmirYnn0d{Bj[XOjbDDpcpZie2mxbjDhcoQhemWmdXPl[EBVT0ZvzsKzMYlv\HWlZXSgbY53[XOrb36geI8h[mG|YXygcIV3\Wy| NI\3RWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK1O|czQSd-MkWyOVc4Ojl:L3G+
HCT116  NYjQZ5RoTnWwY4Tpc44hSXO|YYm= MVmxNEDDvU1? NIroZoMzPCCq NUfUXZRR[2G3c3XzJIEheDV|LXTldIVv\GWwdDD0[ZRz[XCub3nkJGcyNWG{cnXzeEBqdiCmaYDsc4llKEiFVEGxOkBkdG:wZYOgSFMh[W6mIFS4 Mn\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|OECwOVUoRjJ3M{iwNFU2RC:jPh?=
A2780 MkfYSpVv[3Srb36gRZN{[Xl? MYixNOKh|ryP MoXSNlFpyqB? MYTEUXNQ M3HB[oRm[3KnYYPld{B1cGViRn;4UVEhdGW4ZXzz M1fxOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEK2OVQ5Lz5{NUSyOlU1QDxxYU6=
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A2780 NGP0PWVHfW6ldHnvckBCe3OjeR?= NWDVeHBbOTEEoN88US=> MmXmNlFpyqB? NFf0OotFVVOR MlHQbY5kemWjc3XzJJA2OyCycn;0[YlvKGyndnXsdy=> NFvpRpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSyOlU1QCd-MkW0NlY2PDh:L3G+
NCI-H23 NHXzcnNHfW6ldHnvckBCe3OjeR?= NI\CNGUyOMLizszN NIniO4gzOWkEoB?= MVvEUXNQ M4fy[4lv[3KnYYPld{BxPTNicILveIVqdiCuZY\lcJM> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR{NkW0PEc,OjV2Mk[1OFg9N2F-
OVCAR10 MYPGeY5kfGmxbjDBd5NigQ>? NWHLWmhnOTEEoN88US=> M1LrXFIycMLi NXfm[o5vTE2VTx?= M2nJVYlv[3KnYYPld{BxPTNicILveIVqdiCuZY\lcJM> NU[3XWI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0NlY2PDhpPkK1OFI3PTR6PD;hQi=>
MCF-7 MknxSpVv[3Srb36gRZN{[Xl? MljoNVDDqM7:TR?= MkDUNE0zPCCq MmDubY5lfWOnczDwOVMh[W6mIICyNU9EcXBz MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6MkO3N{c,OjV2OEKzO|M9N2F-
SMMC-7721 MV7GeY5kfGmxbjDBd5NigQ>? M1rHPFExKM7:TR?= M{jONlM3KGh? MYXEUXNQ MV\jZZV{\XNidHjlJIVkfG:yaXOg[ZhxemW|c3nvckBw\iCLRlmxOi=> M3jCb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUS0N|YyLz5{NUW0OFM3OTxxYU6=
SMMC-7721 Mm\oSpVv[3Srb36gRZN{[Xl? MYWxNEDPxE1? NYm5Zol1PDhiaB?= MXPEUXNQ NGGxSZhqdmO{ZXHz[ZMhfGinIHX4dJJme3Orb36gcIV3\Wxib3dCpGlHVkJzwrDtVm5C NYe1[ZhbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OFQ{PjFpPkK1OVQ1OzZzPD;hQi=>
SMMC-7721 M4TmUWZ2dmO2aX;uJGF{e2G7 M3XRSFExKM7:TR?= MUW0PEBp M2XkfmROW09? Mn;ubY5lfWOnczD0bIUh[2i{b33heIlvNWKxdX7kJJBzd3SnaX6gTWZKOTZidH:gdIFzfGmjbHz5JIxw[2GuaYrlJIlvKHSqZTDjfZRweGyjc35CpC=> NGrkfoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW0OFM3OSd-MkW1OFQ{PjF:L3G+
SMMC-7721 NYTnOYsyTnWwY4Tpc44hSXO|YYm= NVTzdnJQOTBizszN M{nNcFQ5KGh? MkDUSG1UVw>? NVX3NI5Z[2G3c3XzJGRPSSCGU1Kg[IFu[Wen MmXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NESzOlEoRjJ3NUS0N|YyRC:jPh?=
DU4475  M2nBOGZ2dmO2aX;uJGF{e2G7 MYq1M|ExNzJyIN88US=> NEDqOXAzPMLiaB?= NWL0cIg5\G:5boLl[5Vt[XSnczDUc4NiNTFiZH;z[UBl\XCnbnTlcpRtgQ>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV2N{G3OEc,OjV3NEexO|Q9N2F-
MCF-7  NWPSfY5ETnWwY4Tpc44hSXO|YYm= MXixNOKh|ryP NV\jPFVPTE2VTx?= NGfse3FqdmirYnn0d{BkgWOuaX6gSFEh[W6mIFTpZ4VzyqB? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTdyMkewN{c,OjV5MEK3NFM9N2F-
C2C12 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjoO4gyOMLizszNxsA> MVSyOE81QC95MjDo MlmxSG1UVw>? NH[0TIdqdmirYnn0d{Bk\WyuczDwdo9tcW[ncnH0bY9vKGGwZDDkbYZn\XKnboTpZZRqd25? MmXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{G3PVQoRjJ3OEexO|k1RC:jPh?=
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CRL-5908 NVLLVlhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zXSFI1KGh? NHfuU2hKSzVyPUO4MlcyKMLzIEKuOFMh|ryP NVW4dZFMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xNlUzOzBpPkK2NVI2OjNyPD;hQi=>
A549-920 M2jtNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37lT|I1KGh? MUnJR|UxRTN|Lki1JOKyKDRwOESg{txO NXnlbHpDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xNlUzOzBpPkK2NVI2OjNyPD;hQi=>
A549-NTC NITQeVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHYcY4zPCCq NH7yUGJKSzVyPUG5MlQzKMLzIEGuPVYh|ryP M4T2PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUK1NlMxLz5{NkGyOVI{ODxxYU6=
A549 NXTZXYs2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7p[IkzPCCq M1nvdmlEPTB;MUeuOlghyrFiND61NkDPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF{NUKzNEc,OjZzMkWyN|A9N2F-
C666-1 NYLOdYJkSXCxcITvd4l{KEG|c3H5 MXexNEDDvU1? MlO3OFgwPzJiaB?= M37yT2ROW09? MoTkd4Vve2m2aYrld{BEPjZ4LUGgZ4VtdHNidH:gZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz MlfQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{NUK1O|UoRjJ4MkWyOVc2RC:jPh?=
C666-1  NIHBSYVHfW6ldHnvckBCe3OjeR?= MlrhNVAhyrWP MUSyOEBp NGjHWlJFVVOR NH2xfoRi[3SrdnH0[ZMhfGinIIC1N{Bx[XSqd3H5MEB2eHKnZ4XsZZRqdmdicEWzMEBxOjFiYX7kJG1ldTJ? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ3MkW3OUc,OjZ{NUK1O|U9N2F-
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A2780 MWTBdI9xfG:|aYOgRZN{[Xl? M3r4dFUwOTBxMkCg{txO NXTyNGlHOjRiaB?= NHO1eIRmdmijbnPld{BieG:ydH;zbZMhcW6mdXP0bY9vKGK7IFSyOllJN0VzOUXSJI93\XJicnjUVmFKVA>? M4HmWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO2NVQ4Lz5{NEGzOlE1PzxxYU6=
H460 MnOwRZBweHSxc3nzJGF{e2G7 M{Pac|UwOTBxMkCg{txO NX7QcZloOjRiaB?= NEDSe3NmdmijbnPld{BieG:ydH;zbZMhcW6mdXP0bY9vKGK7IFSyOllJN0VzOUXSJI93\XJicnjUVmFKVA>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|NkG0O{c,OjRzM{[xOFc9N2F-
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U87MG MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{PLUlQ5KGi{cx?= NUXSS|hCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFdOTyClZXzsd{BmgHC{ZYPzbY5oKHerbHSgeJlx\SCyNUOgZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeTygTWM2OCB;IE[uOUDPxE1w NEnHcYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C1NFc5Oid-MkewOVA4QDJ:L3G+
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SW620 MmDCR5l1d3SxeHnjbZR6KGG|c3H5 MnnPO|IhcHK| NWnrSIRxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW1d4MkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDBwM{ig{txONg>? NEjwUG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQTF|M{SvK|5EcEWPQly8M4E,
A549 M3rQUGN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\ud4Q4OiCqcoO= M4HGNGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFAvPDFizszNMi=> NEXZOoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQTF|M{SvK|5EcEWPQly8M4E,
SJSA1 MYDDfZRwfG:6aXPpeJkh[XO|YYm= NIqyN|I4OiCqcoO= MnruR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2pUSTFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFEvQDFizszNMi=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPVE{OzRxJ{7DbGVOSkx:L3G+
HCT116 NETqRVREgXSxdH;4bYNqfHliYYPzZZk> NVX6VVZYPzJiaILz NE\hUYdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDRwNkOg{txONg>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPVE{OzRxJ{7DbGVOSkx:L3G+
PC3 MXXDfZRwfG:6aXPpeJkh[XO|YYm= MVW3NkBpenN? MlHrR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGM{KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTC2MlM4KM7:TT6= M3XnWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE6OTN|ND:nQmNpTU2ETEyvZV4>
Assay
Methods Test Index PMID
Western blot MDM2 / p53 / ALKBH2 / p21 / PUMA ; RRM1 / RRM2 / p53R2 / p21 / p53 / pS6 / S6 23258843 28507282
Immunofluorescence Lamin A / Lamin C / p16 / H3K9me3 ; Merlin / cyclin D1 / p53 / MDM2 ; p53 30728349 30274821 24286312
Growth inhibition assay Cell viability 29286113
In vivo Oral administration of Nutlin-3 at 200 mg/kg twice daily for 3 weeks significantly inhibits the tumor growth of SJAS-1 xenografts by 90%, comparable with the effect of doxorubicin treatment with 81% inhibition of tumor growth. [1]

Protocol (from reference)

Kinase Assay:

[1]

  • Biacore study:

    Competition assay is performed on a Biacore S51. A Series S Sensor chip CM5 is utilized for the immobilization of a PentaHis antibody for capture of the His-tagged p53. The level of capture is ~200 response units (1 response unit corresponds to 1 pg of protein per mm2). The concentration of MDM2 protein is kept constant at 300 nM. Nutlin-3 is dissolved in DMSO at 10 mM and further diluted to make a concentration series of Nutlin-3 in each MDM2 test sample. The assay is run at 25 °C in running buffer (10 mM Hepes, 0.15 M NaCl, 2% DMSO). MDM2-p53 binding in the presence of Nutlin-3 is calculated as a percentage of binding in the absence of Nutlin-3 and IC50 is calculate

Cell Research:

[1]

  • Cell lines: HCT116, RKO, SJSA-1, SW480, and MDA-MB-435
  • Concentrations: Dissolved in DMSO, final concentrations ~ 30 μM
  • Incubation Time: 8, 24, and 48 hours
  • Method:

    Cells are exposed to various concentrations of Nutlin-3 for 8, 24 and 48 hours. The transcriptional levels of p21 and MDM2 genes are analyzed by real-time PCR, and protein levels by western blotting. Cell viability is measured by the MTT assay. Cell apoptosis is determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining with flow cytometry and fluorescence microscopy.

Animal Research:

[1]

  • Animal Models: Athymic female nude mice (Nu/Nu-nuBR) injected subcutaneously with SJSA-1 cells
  • Dosages: 200 mg/kg
  • Administration: Orally, twice a day

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 581.5
Formula

C30H30Cl2N4O4

CAS No. 890090-75-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)OC1=C(C=CC(=C1)OC)C2=NC(C(N2C(=O)N3CCNC(=O)C3)C4=CC=C(C=C4)Cl)C5=CC=C(C=C5)Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is this a racemic mixture of Nutlin-3a and Nutlin-3b or just the Nutlin-3a enantiomer?

Answer:
It is a racemate.

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