YH239-EE

Catalog No.S7489

For research use only.

YH239-EE, the ethyl ester of YH239, is a potent p53-MDM2 antagonist and an apoptosis inducer.

YH239-EE Chemical Structure

CAS No. 1364488-67-4

Selleck's YH239-EE has been cited by 1 Publication

Purity & Quality Control

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Biological Activity

Description YH239-EE, the ethyl ester of YH239, is a potent p53-MDM2 antagonist and an apoptosis inducer.
Targets
p53-MDM2 interaction [1]
In vitro

YH239-EE inhibits the growth of OCI-AML-3 cells with wild type p53 by inhibiting the p53-MDM2 interaction. YH239-EE induces cell cycle arrest and causes potent cell apoptosis via activation of p53 and downstream targets in four AML cells (OCI-AML-3 and MOLM-13 with wt p53, NB4 with p53 mutation, and HL60 with p53 deletion). [1]

Protocol (from reference)

Cell Research:[1]
  • Cell lines: OCI-AML-3 cells
  • Concentrations: ~50 μM
  • Incubation Time: 72 hours
  • Method: Cell lines are harvested in log-phase growth and seeded following exposure to p53-MDM2 antagonists. Cell number and viability are determined by staining cells with Trypan blue 0.4% and counted with Countess slides.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 504.41
Formula

C25H27Cl2N3O4

CAS No. 1364488-67-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCOC(=O)C1=C(C2=C(N1)C=C(C=C2)Cl)C(C(=O)NC(C)(C)C)N(CC3=CC=C(C=C3)Cl)C=O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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