Busulfan (NSC-750)

Catalog No.S1692

For research use only.

Busulfan (NSC-750) is a cell cycle non-specific alkylating antineoplastic agent. Busulfan induces apoptosis.

Busulfan (NSC-750) Chemical Structure

CAS No. 55-98-1

Selleck's Busulfan (NSC-750) has been cited by 14 publications

Purity & Quality Control

Choose Selective DNA alkylator Inhibitors

Biological Activity

Description Busulfan (NSC-750) is a cell cycle non-specific alkylating antineoplastic agent. Busulfan induces apoptosis.
In vitro

Busulfan inhibits the cobblestone area-forming cell frequency but fails to cause a significant increase in apoptosis in hematopoietic stem cell alike cells and progenitors. Busulfan inhibits the hematopoietic function of HSC alike cells and progenitors via an apoptosis-independent mechanism. Busulfan induces bone marrow hematopoietic cell senescence associated with an increased expression of p16Ink4a and p19Arf in a time-dependent manner. [1] Busulfan, an alkylating agent that causes DNA damage by cross-linking DNAs and DNA and proteins, induces senescence in normal human diploid WI38 fibroblasts through the extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (p38 MAPK) cascade independent of the p53-DNA damage pathway. Busulfan induces a transient reduction in GSH but a continuous increase in ROS production. [2] Busulfan-induced hypophosphorylation of Rb prevents apoptosis of spermatogonial stem cells by inhibiting PCNA expression in testicular cells. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SK-N-SH cells Mln5VJJwdGmoZYLheIlwdiCjc4PhfS=> NXvDdGNoOTBvMUCwJO69VQ>? NVjjTWRJPzJiaB?= M1ry[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1utUk1UUCClZXzsd{BifCBzMDD0c{AyODBidV2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNEWzNkc,OjR6MUS1N|I9N2F-
SiMa cells M1HnW3Bzd2yrZnXyZZRqd25iYYPzZZk> MlTxNVAuOTByIN88US=> MYK3NkBp NGq1cVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPpUYEh[2WubIOgZZQhOTBidH:gNVAxKHWPIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[Xl? NFH4WVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixOFU{Oid-MkS4NVQ2OzJ:L3G+
Kelly cells  NU\0b4l1WHKxbHnm[ZJifGmxbjDhd5NigQ>? MUexNE0yODBizszN Ml\xO|IhcA>? MlOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNZXzsfUBk\WyuczDheEAyOCC2bzCxNFAhfU1iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>? M3m3ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG0OVMzLz5{NEixOFU{OjxxYU6=
LS cells NHLT[ZZRem:uaX\ldoF1cW:wIHHzd4F6 Mmm0NVAuOTByIN88US=> M33wdFczKGh? NXnCO4FjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMV{Bk\WyuczDheEAyOCC2bzCxNFAhfU1iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNEWzNkc,OjR6MUS1N|I9N2F-
K562 NH[3UIlCeG:ydH;zbZMh[XO|YYm= NIrre4E1QCCqcoO= M{TUSmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbY1ifGmwaXKgcYV{gWyjdHWtdoV{cXO2YX70JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiNEigbJJ{ MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN|OUS1OUc,OTh|M{m0OVU9N2F-
SK-N-MC MmTGdWhVWyCjc4PhfS=> MV3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NEjX[lA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 MoD6dWhVWyCjc4PhfS=> MWLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
A673 NE[xe2pyUFSVIHHzd4F6 NHLwZVByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? MnLUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Assay
Methods Test Index PMID
Western blot p-Y534 AR / AR ; Ack1 / Src 27904688
Growth inhibition assay Cell viability 24815002
In vivo Busulfan-treated mice exhibit a marked increase in apoptosis and a decrease in testis weight. Busulfan is administered at the rate of 40 mg/kg body weight to induce a maximal number of apoptotic cells while minimizing the number of necrotic cells. [3] Busulfan conditioning and irradiation results in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis in NOD/SCID mice. [4] Busulfan-transplanted mice has slow and incomplete lymphoid engraftment. Busulfan (20 mg/kg to 100 mg/kg) provides dose-dependent congenic lymphoid reconstitution in mice. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 246.3
Formula

C6H14O6S2

Density 1.4 g/mL
CAS No. 55-98-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CS(=O)(=O)OCCCCOS(=O)(=O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04451200 Not yet recruiting Drug: Busulfan Injection Acute Leukemia|Mielodysplasic Syndrome|Myeloproliferative Neoplasm Institut Paoli-Calmettes November 2020 Phase 2
NCT03601286 Recruiting Drug: Lentiviral vector transduced CD34+ cells Severe Combined Immunodeficiency X-Linked Great Ormond Street Hospital for Children NHS Foundation Trust December 21 2018 Phase 1
NCT03235973 Unknown status Drug: Fludarabine-Cladribine-Busulfan conditioning regimen Leukemia Myeloid Acute|Leukemia Lymphoblastic Acute Institut Paoli-Calmettes April 28 2018 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.

Frequently Asked Questions

Question 1:
How can I reconstitute the drug for in vivo studies?

Answer:
This compound also can be dissolved in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG and Tween. After they mixed well, dilute with water. S1692 in this formulation is not suitable for long-term storage. Please make the fresh solution each time before use.

Tags: buy Busulfan (NSC-750) | Busulfan (NSC-750) ic50 | Busulfan (NSC-750) price | Busulfan (NSC-750) cost | Busulfan (NSC-750) solubility dmso | Busulfan (NSC-750) purchase | Busulfan (NSC-750) manufacturer | Busulfan (NSC-750) research buy | Busulfan (NSC-750) order | Busulfan (NSC-750) mouse | Busulfan (NSC-750) chemical structure | Busulfan (NSC-750) mw | Busulfan (NSC-750) molecular weight | Busulfan (NSC-750) datasheet | Busulfan (NSC-750) supplier | Busulfan (NSC-750) in vitro | Busulfan (NSC-750) cell line | Busulfan (NSC-750) concentration | Busulfan (NSC-750) nmr