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Busulfan (NSC-750)

Name: Busulfan (NSC-750)
Brand: Selleck Chemicals
SKU: S1692
Rating: 5 (14 reviews)

Catalog No.S1692

For research use only.

Busulfan (NSC-750) is a cell cycle non-specific alkylating antineoplastic agent. Busulfan induces apoptosis.

CAS No. 55-98-1

Selleck's Busulfan (NSC-750) has been cited by 14 publications

Purity & Quality Control

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Biological Activity

Description

Busulfan (NSC-750) is a cell cycle non-specific alkylating antineoplastic agent. Busulfan induces apoptosis.

In vitro

Busulfan inhibits the cobblestone area-forming cell frequency but fails to cause a significant increase in apoptosis in hematopoietic stem cell alike cells and progenitors. Busulfan inhibits the hematopoietic function of HSC alike cells and progenitors via an apoptosis-independent mechanism. Busulfan induces bone marrow hematopoietic cell senescence associated with an increased expression of p16^Ink4a and p19^Arf in a time-dependent manner. ^[1] Busulfan, an alkylating agent that causes DNA damage by cross-linking DNAs and DNA and proteins, induces senescence in normal human diploid WI38 fibroblasts through the extracellular signal-regulated kinase (Erk) and p38 mitogen-activated protein kinase (p38 MAPK) cascade independent of the p53-DNA damage pathway. Busulfan induces a transient reduction in GSH but a continuous increase in ROS production. ^[2] Busulfan-induced hypophosphorylation of Rb prevents apoptosis of spermatogonial stem cells by inhibiting PCNA expression in testicular cells. ^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

SK-N-SH cells	Mln5VJJwdGmoZYLheIlwdiCjc4PhfS=>	NXvDdGNoOTBvMUCwJO69VQ>?	NVjjTWRJPzJiaB?=	M1ry[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1utUk1UUCClZXzsd{BifCBzMDD0c{AyODBidV2gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeR?=	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNEWzNkc,OjR6MUS1N\|I9N2F-
SiMa cells	M1HnW3Bzd2yrZnXyZZRqd25iYYPzZZk>	MlTxNVAuOTByIN88US=>	MYK3NkBp	NGq1cVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPpUYEh[2WubIOgZZQhOTBidH:gNVAxKHWPIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[Xl?	NFH4WVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixOFU{Oid-MkS4NVQ2OzJ:L3G+
Kelly cells	NU\0b4l1WHKxbHnm[ZJifGmxbjDhd5NigQ>?	MUexNE0yODBizszN	Ml\xO\|IhcA>?	MlOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCNZXzsfUBk\WyuczDheEAyOCC2bzCxNFAhfU1iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>?	M3m3ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG0OVMzLz5{NEixOFU{OjxxYU6=
LS cells	NHLT[ZZRem:uaX\ldoF1cW:wIHHzd4F6	Mmm0NVAuOTByIN88US=>	M33wdFczKGh?	NXnCO4FjSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMV{Bk\WyuczDheEAyOCC2bzCxNFAhfU1iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNEWzNkc,OjR6MUS1N\|I9N2F-
K562	NH[3UIlCeG:ydH;zbZMh[XO\|YYm=		NIrre4E1QCCqcoO=	M{TUSmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbY1ifGmwaXKgcYV{gWyjdHWtdoV{cXO2YX70JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiNEigbJJ{	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN\|OUS1OUc,OTh\|M{m0OVU9N2F-
SK-N-MC	MmTGdWhVWyCjc4PhfS=>			MV3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?=	NEjX[lA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
NB-EBc1	MoD6dWhVWyCjc4PhfS=>			MWLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?=	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
A673	NE[xe2pyUFSVIHHzd4F6			NHLwZVByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd\|IHPlcIx{MQ>?	MnLUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	p-Y534 AR / AR ; Ack1 / Src	27904688
Growth inhibition assay	Cell viability	24815002

In vivo

Busulfan-treated mice exhibit a marked increase in apoptosis and a decrease in testis weight. Busulfan is administered at the rate of 40 mg/kg body weight to induce a maximal number of apoptotic cells while minimizing the number of necrotic cells. ^[3] Busulfan conditioning and irradiation results in comparable sensitivity of HSC detection as evaluated by limiting dilution analysis in NOD/SCID mice. ^[4] Busulfan-transplanted mice has slow and incomplete lymphoid engraftment. Busulfan (20 mg/kg to 100 mg/kg) provides dose-dependent congenic lymphoid reconstitution in mice. ^[5]

Protocol (from reference)

References

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Solubility (25°C)

In vitro


In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	5mg/mL

Chemical Information

Molecular Weight	246.3
Formula	C₆H₁₄O₆S₂
Density	1.4 g/mL
CAS No.	55-98-1
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CS(=O)(=O)OCCCCOS(=O)(=O)C

Download Busulfan (NSC-750) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04451200	Not yet recruiting	Drug: Busulfan Injection	Acute Leukemia\|Mielodysplasic Syndrome\|Myeloproliferative Neoplasm	Institut Paoli-Calmettes	November 2020	Phase 2
NCT03601286	Recruiting	Drug: Lentiviral vector transduced CD34+ cells	Severe Combined Immunodeficiency X-Linked	Great Ormond Street Hospital for Children NHS Foundation Trust	December 21 2018	Phase 1
NCT03235973	Unknown status	Drug: Fludarabine-Cladribine-Busulfan conditioning regimen	Leukemia Myeloid Acute\|Leukemia Lymphoblastic Acute	Institut Paoli-Calmettes	April 28 2018	Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
How can I reconstitute the drug for in vivo studies?

Answer:
This compound also can be dissolved in 2% DMSO+30% PEG 300+5% Tween 80+ddH2O at 5mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG and Tween. After they mixed well, dilute with water. S1692 in this formulation is not suitable for long-term storage. Please make the fresh solution each time before use.

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