ZM241385

Catalog No.S8105

For research use only.

ZM-241385 is a high affinity antagonist ligand selective for the adenosine A2A receptor.

ZM241385 Chemical Structure

CAS No. 139180-30-6

Selleck's ZM241385 has been cited by 7 Publications

1 Customer Review

Purity & Quality Control

Choose Selective Adenosine Receptor Inhibitors

Biological Activity

Description ZM-241385 is a high affinity antagonist ligand selective for the adenosine A2A receptor.
Targets
adenosine A2A receptor [3]
In vitro

ZM 241385 has high affinity for A2a receptors. In rat phaeochromocytoma cell membranes, ZM 241385 displaces binding of tritiated 5'-N-ethylcarboxamidoadenosine (NECA) with a pIC50 of 9.52[3]. ZM241385 delays the appearance of anoxic depolarization (AD), a phenomenon strictly related to cell damage and death, protect from the synaptic activity depression brought about by a severe (7 min) OGD period, and protect CA1 neuron and astrocyte from injury[4].

In vivo ZM-241385 significantly blunts the hypotensive effects of CCPA and NECA without altering the bradycardia induced by these agonists. It blocks the infarct size-reducing effects(cardioprotective effects) of this two distinct adenosine receptor agonists[1].

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: PC12 cells
  • Concentrations: 1 μM
  • Incubation Time: 24 h
  • Method:

    Nitric oxide (NO) production is assessed by measuring the amount of nitrite, a stable metabolic product of NO that provides an indirect measurement of NO, by using the Griess diazotization reaction. Briefly, 24 h after incubation with 1.0 µM CG21680 or 1.0 µM ZM241385 or exposure to SMF, samples of medium (150 µl) are collected from cells and mixed with 130 µl dH2O and with 20 µl Griess reagent using instruction supplied by the manufacturer. After a 30 min incubation period at room temperature, the samples are evaluated spectrophotometrically at 548 nm and OD values-in comparison with a standard curve is determined in culture medium by using serial dilutions of sodium nitrite-represented total stable metabolites of NO.

Animal Research:

[1]

  • Animal Models: Adult Sprague-Dawley rats 
  • Dosages: 1.5 mg/kg
  • Administration: i.v.

Solubility (25°C)

In vitro

DMSO 67 mg/mL
(198.61 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 337.34
Formula

C16H15N7O2

CAS No. 139180-30-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=COC(=C1)C2=NN3C(=NC(=NC3=N2)NCCC4=CC=C(C=C4)O)N

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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