For research use only.
Catalog No.S3114 Synonyms: Ascorbic acid
CAS No. 50-81-7
Vitamin C (Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.
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|Description||Vitamin C (Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.|
Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice.  Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells.  Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells.  Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. 
|In vivo||Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. |
-  Bram S, et al. Nature, 1980, 284(5757), 629-631.
-  Witenberg B, et al. Biochem Pharmacol, 1999, 57(7), 823-832.
-  Guaiquil VH, et al. J Biol Chem, 2001, 276(44), 40955-40961.
|In vitro||DMSO||35 mg/mL (198.72 mM)|
|Water||35 mg/mL (198.72 mM)|
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
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Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04505878||Not yet recruiting||Drug: Vitamin C||Primary Graft Dysfunction|Lung Transplant; Complications||University of Wisconsin Madison||April 2021||Phase 2|
|NCT04641663||Not yet recruiting||Dietary Supplement: Multi-target Dietary Supplement (MTDS)||Aging|Age-related Cognitive Decline||Douglas Boreham|McMaster University|Northern Ontario School of Medicine||January 4 2021||Not Applicable|
|NCT04565392||Not yet recruiting||Drug: Famotidine 20 MG||Covid19||drpykessupplements.com||December 1 2020||Phase 4|
|NCT04567823||Recruiting||Dietary Supplement: Smoothie with microalgae||Nutrient Deficiency||University of Jena|German Federal Ministry of Education and Research||November 9 2020||Not Applicable|
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