For research use only.
Catalog No.S3114 Synonyms: Ascorbic acid
CAS No. 50-81-7
Vitamin C (Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.
Selleck's Vitamin C has been cited by 5 publications
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|Description||Vitamin C (Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.|
Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice.  Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells.  Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells.  Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. 
|In vivo||Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. |
-  Bram S, et al. Nature, 1980, 284(5757), 629-631.
-  Witenberg B, et al. Biochem Pharmacol, 1999, 57(7), 823-832.
-  Guaiquil VH, et al. J Biol Chem, 2001, 276(44), 40955-40961.
|In vitro||DMSO||35 mg/mL (198.72 mM)|
|Water||35 mg/mL (198.72 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04505878||Not yet recruiting||Drug: Vitamin C||Primary Graft Dysfunction|Lung Transplant; Complications||University of Wisconsin Madison||October 2021||Phase 2|
|NCT04999137||Not yet recruiting||Drug: Vitamin C|Drug: Vitamin B1||Sepsis|Septic Shock|HIV||Liverpool School of Tropical Medicine|Infectious Diseases Institute Uganda|Walimu|University of Copenhagen|University of Liverpool||August 2 2021||Phase 2|
|NCT04641663||Recruiting||Dietary Supplement: Multi-target Dietary Supplement (MTDS)||Aging|Age-related Cognitive Decline||Douglas Boreham|McMaster University|Northern Ontario School of Medicine||July 15 2021||Not Applicable|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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