Name: L-Ascorbic Acid (Vitamin C)
Brand: Selleck Chemicals
SKU: S3114
Availability: InStock
Rating: 5 (21 reviews)

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Quality Control

Batch: S311403 Water]350 mg/mL]true]DMSO]35 mg/mL]false]Ethanol]Insoluble]false Purity: 99.91%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS/MSDS
Datasheet

99.97

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Antioxidant Inhibitors	Elamipretide (SS-31, MTP-131) EGCG ((-)-Epigallocatechin Gallate) Tangeretin Silymarin Gipsoside (+)-Catechin Hydroxygenkwanin Rutin (trihydrate) Oleuropein (-)Epicatechin

Solubility

In vitro
Batch:

Water : 350 mg/mL (超声加热十分钟)

DMSO : 35 mg/mL (198.72 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.750mg/ml (9.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 35 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.580mg/ml (3.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	176.12	Formula	C₆H₈O₆	Storage (From the date of receipt)	3 years -20°C powder (seal)
CAS No.	50-81-7	Download SDF		Storage of Stock Solutions	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms	L-Ascorbic acid			SMILES	C(C(C1C(=C(C(=O)O1)O)O)O)O

Read more about storage stability CAS number SMILES

Mechanism of Action

In vitro	Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. This compound loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. This chemical is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. It inhibits the formation of ROS in both normal and GSH-depleted cells. Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected.
In vivo	Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of this compound tested.
References	[1] https://pubmed.ncbi.nlm.nih.gov/7366735/ [2] https://pubmed.ncbi.nlm.nih.gov/10075088/ [3] https://pubmed.ncbi.nlm.nih.gov/11533037/ [4] https://pubmed.ncbi.nlm.nih.gov/11291594/ [5] https://pubmed.ncbi.nlm.nih.gov/20412793/ [6] https://pubmed.ncbi.nlm.nih.gov/25015208/ [7] https://pubmed.ncbi.nlm.nih.gov/15745442/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-06-26)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06068543	SUSPENDED	Frailty; Inflammation	University of Rochester	2024-10-25	PHASE2
NCT07765667	NOT_YET_RECRUITING	Rectal Adenocarcinoma; High-Risk Cancer; MSS	Sixth Affiliated Hospital, Sun Yat-sen University	2026-09-01	PHASE2
NCT06968936	RECRUITING	Anemia; Iron Deficiencies; Cardiac Surgery	Second Affiliated Hospital, Zhejiang University, School of Medicine	2026-03-27
NCT06009744	RECRUITING	Enterocutaneous Fistulas	Hospital Juarez de Mexico	2023-08
NCT04150042	RECRUITING	Pancreatic Adenocarcinoma Metastatic; BRCA1 Mutation; BRCA2 Mutation; Pancreatic Acinar Cell Carcinoma; Pancreatic Ductal Adenocarcinoma; Pancreatic Cancer; Metastatic Pancreatic Cancer; Metastatic Pancreatic Ductal Adenocarcinoma; Breast Cancer Metastatic; Breast Cancer Stage IV; Pancreatic Cancer Stage IV; HER2-negative Breast Cancer; HER2 Negative Breast Carcinoma; Adenocarcinoma of the Breast; PALB2 Gene Mutation; Pancreas Cancer, Metastatic; Pancreas Cancer, Recurrent; Pancreas Cancer; Stage IV Pancreatic Cancer; Stage 4 Pancreatic Cancer	General Oncology, Inc.	2021-01-13	PHASE1
NCT07197749	NOT_YET_RECRUITING	Graft-versus-host-disease (GVHD); Gastrointestinal	Baylor College of Medicine	2026-11	EARLY_PHASE1

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L-Ascorbic Acid (Vitamin C) Antioxidant

Chemical Structure

Molecular Weight: 176.12