Trolox

For research use only.

Catalog No.S3665

1 publication

Trolox Chemical Structure

Molecular Weight(MW): 250.29

Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.

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Selleck's Trolox has been cited by 1 publication

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Biological Activity

Description Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.
In vitro

Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid) is a vitamin E analog and a direct scavenger of peroxyl and alkoxyl radicals. Trolox impairs PT4 cell survival, has minor effects on ROS levels and causes wide deregulation of cell cycle genes[1]. Trolox enhances As2O3-mediated apoptosis in APL, myeloma, and breast cancer cells. Trolox inhibits DNA damage formation induced by singlet oxygen in human lymphoblast WTK-1 cells and protects red blood cells during photodynamic treatment[2].

In vivo After cerebral ischemia reperfusion, Trolox prescription increases anti-apoptotic proteins and decreased proapoptotic proteins thus protects neurons of the hippocampus and caused improvement of memory[3].

Protocol

Cell Research:[2]
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  • Cell lines: NB4, IM9, and AsR2 cells
  • Concentrations: 100 μM
  • Incubation Time: 6 days
  • Method: NB4, IM9, and AsR2 cells are seeded at 1 × 105 cells/mL in 24-well plates. Cells are treated with various concentrations of As2O3 or doxorubicin, alone or in combination with 100 μM trolox for 6 days. Viable cells are counted by trypan blue exclusion on days 1, 3, and 6. All cells are maintained at a density lower than 1 × 106 cells/mL through dilution as required, and media ± treatment is replaced every third day. MCF-7, MDA-231, and T47D are seeded in 24-well plates at a density of 4000 cells/well. The next day, fresh media containing As2O3 ± trolox is added. On the days indicated, cells are fixed in 10% trichloroacetic acid and subsequently stained with sulforhodamine B (SRB). Bound SRB is solubilized in 10 mM unbuffered Tris (tris(hydroxymethyl)aminomethane), and optical density is measured at 570 nm in a microplate reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (199.76 mM)
Ethanol 50 mg/mL (199.76 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 250.29
Formula

C14H18O4

CAS No. 53188-07-1
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(C)C2=C(CCC(C)(O2)C(O)=O)C(=C1O)C

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04053569 Active not recruiting Dietary Supplement: Table Grape supplement Metabolic Syndrome Protection Against Azienda Ospedaliera Specializzata in Gastroenterologia Saverio de Bellis October 1 2019 Not Applicable
NCT03707067 Recruiting Device: Compression garments|Other: Sham recovery drink Muscle Damage St Mary''s University College|Modern Pentathlon GB November 30 2018 Not Applicable
NCT03938584 Recruiting Dietary Supplement: Vitamin C|Other: Placebo Vitamin C Deficiency|Smoking|Surgery|Surgery--Complications|Wound|Wound Complication|Wound Infection|Wound Dehiscence|Wound of Skin|Mandible Fracture|Mandible Open Fracture|Mandible Closed Fracture|Mandibular Fractures|Ascorbic Acid Deficiency|Oxidative Stress|Inflammation Hennepin Healthcare Research Institute|Regions Hospital|University of Minnesota October 12 2017 Not Applicable
NCT03685552 Completed Other: Prog: Purify-2 Gastrointestinal Symptoms Nature''s Sunshine Products Inc. August 3 2017 Not Applicable

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID