Molecular Weight(MW): 416.57
Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
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(A, B) Indicated HIV-1 latency cells (A. J-LAT 10.6; B. CA5) were treated with levosimendan or spironolactone (5, 10, or 20 μM) in combination with TNFα (10 ng/ml). After 24 hr, mRNA was extracted, and reverse transcribed to cDNA for measurement of HIV-1 initiated or elongated (proximal [Pro], intermediate [Int], and distal [Dis]) transcripts by qPCR. Results were normalized to DMSO control. Values represent the mean ± s.d. (n = 4-6).
Antiviral Res, 2017, 146:76-85. Spironolactone purchased from Selleck.
Purity & Quality Control
Choose Selective Androgen Receptor Inhibitors
|Description||Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.|
Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM).  Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. 
|In vivo||Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. |
|In vitro||DMSO||83 mg/mL (199.24 mM)|
|Ethanol||20 mg/mL (48.01 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03314493||Completed||Coronary Artery Calcification|Vascular Calcification||Professor Fernando Figueira Integral Medicine Institute|Federal University of São Paulo||November 7 2014||Phase 3|
|NCT01867294||Completed||Advanced Malignant Neoplasm|Dermatologic Complication||Academic and Community Cancer Research United|National Cancer Institute (NCI)||August 31 2012||Phase 2|
|NCT02235077||Completed||Heart Failure||Duke University|National Heart Lung and Blood Institute (NHLBI)||December 30 2014||Phase 2|
|NCT01712620||Recruiting||Pulmonary Arterial Hypertension||National Institutes of Health Clinical Center (CC)|National Heart Lung and Blood Institute (NHLBI)|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)|University of Pennsylvania|University of Maryland|Washington Hospital Center|New England Medical Center Tufts University School of Medicine||September 25 2012||Phase 1|Phase 2|
|NCT02901184||Recruiting||Heart Failure With Preserved Ejection Fraction||Uppsala University|Karolinska University||November 23 2017||Phase 4|
|NCT03576755||Recruiting||NASH - Nonalcoholic Steatohepatitis||Monika Sarkar MD|University of California San Francisco||October 2018||Phase 1|Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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