Catalog No.S4054

For research use only.

Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.

Spironolactone Chemical Structure

CAS No. 52-01-7

Selleck's Spironolactone has been cited by 8 Publications

2 Customer Reviews

Purity & Quality Control

Choose Selective Androgen Receptor Inhibitors

Other Androgen Receptor Products

Biological Activity

Description Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
Androgen Receptor [1]
77 nM
In vitro

Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2]

In vivo Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 83 mg/mL
(199.24 mM)
Ethanol 20 mg/mL
(48.01 mM)
Water Insoluble

Chemical Information

Molecular Weight 416.57


CAS No. 52-01-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)SC1CC2=CC(=O)CCC2(C3C1C4CCC5(C4(CC3)C)CCC(=O)O5)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05092984 Not yet recruiting Drug: Spironolactone|Drug: Placebo Rheumatoid Arthritis University Hospital Strasbourg France December 2021 Phase 3
NCT04723862 Recruiting Drug: Spironolactone|Drug: Placebo Hyperandrogenism|Polycystic Ovary Syndrome|Puberty University of Virginia|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) February 1 2021 Early Phase 1
NCT03921476 Withdrawn Other: Online survey Acne Vulgaris Northwestern University July 1 2020 --

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Spironolactone | Spironolactone supplier | purchase Spironolactone | Spironolactone cost | Spironolactone manufacturer | order Spironolactone | Spironolactone distributor