Spironolactone

Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.

Spironolactone Chemical Structure

Spironolactone Chemical Structure

CAS: 52-01-7

Selleck's Spironolactone has been cited by 11 Publications

2 Customer Reviews

Purity & Quality Control

Batch: Purity: 99.80%
99.80

Spironolactone Related Products

Choose Selective Androgen Receptor Inhibitors

Biological Activity

Description Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
Targets
Androgen Receptor [1]
77 nM
In vitro
In vitro Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2]
In Vivo
In vivo Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2]
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Chemical Information & Solubility

Molecular Weight 416.57 Formula

C24H32O4S

CAS No. 52-01-7 SDF Download Spironolactone SDF
Smiles CC(=O)SC1CC2=CC(=O)CCC2(C3C1C4CCC5(C4(CC3)C)CCC(=O)O5)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 83 mg/mL ( (199.24 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 27 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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