Name: Spironolactone
Brand: Selleck Chemicals
SKU: S4054
Availability: InStock
Rating: 5 (18 reviews)

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Quality Control

Batch: Purity: 99.80%

99.80

Related Targets	Adrenergic Receptor Estrogen/progestogen Receptor GPR Glucocorticoid Receptor ACE RAAS Progesterone Receptor Opioid Receptor PGES THR
Other Androgen Receptor Inhibitors	Bavdegalutamide (ARV-110) RU58841 Galeterone EPI-001 Cyproterone Acetate 3,3'-Diindolylmethane GSK-2881078 Chlormadinone acetate GDC-2992 JNJ-63576253 (TRC-253)

Solubility

In vitro
Batch:

DMSO : 83 mg/mL (199.24 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 27 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.150mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 83 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.510mg/ml (1.22mM)	Taking the 1 mL working solution as an example, add 50 μL of 10.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	416.57	Formula	C₂₄H₃₂O₄S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	52-01-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(=O)SC1CC2=CC(=O)CCC2(C3C1C4CCC5(C4(CC3)C)CCC(=O)O5)C

Mechanism of Action

Targets/IC₅₀/Ki	Androgen Receptor 77 nM
In vitro	Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). This compound inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol.
In vivo	Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of this compound (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [³H]testosterone.
References	[1] http://www.ncbi.nlm.nih.gov/pubmed/?term=20650892 [2] http://www.ncbi.nlm.nih.gov/pubmed/?term=4376096

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06021860	Not yet recruiting	Edema Due to Heart Failure or Cirrhosis	CMP Development LLC	June 2024	Phase 4
NCT06204640	Not yet recruiting	Atrial Fibrillation Recurrent	University Hospital Caen	March 1 2024	Phase 3
NCT05807139	Recruiting	Alcohol Use Disorder	National Institute on Drug Abuse (NIDA)\|National Institutes of Health Clinical Center (CC)	July 13 2023	Phase 1
NCT05092984	Recruiting	Rheumatoid Arthritis	University Hospital Strasbourg France	June 22 2022	Phase 3
NCT04723862	Recruiting	Hyperandrogenism\|Polycystic Ovary Syndrome\|Puberty	University of Virginia\|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)	November 12 2021	Early Phase 1

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Handling Instructions

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Spironolactone Androgen Receptor antagonist

Chemical Structure

Molecular Weight: 416.57