3,3'-Diindolylmethane

Catalog No.S4728 Synonyms: DIM

For research use only.

3,3'-Diindolylmethane(DIM) is a major digestive product of indole-3-carbinol, a potential anticancer component of cruciferous vegetables. 3,3'-Diindolylmethane is a strong, pure androgen receptor (AR) antagonist.

3,3'-Diindolylmethane Chemical Structure

CAS No. 1968-05-4

Selleck's 3,3'-Diindolylmethane has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description 3,3'-Diindolylmethane(DIM) is a major digestive product of indole-3-carbinol, a potential anticancer component of cruciferous vegetables. 3,3'-Diindolylmethane is a strong, pure androgen receptor (AR) antagonist.
Targets
Androgen Receptor [1]
()
In vitro

DIM is a potent radioprotector and mitigator that functions by stimulating an ATM-driven DDR-like response and NF-κB survival signaling. DIM can inhibit invasion, angiogenesis, and proliferation and induce apoptosis in tumor cells by modulating signaling pathways involving AKT, NF-κB, and FOXO3. It can also inhibit estrogen-inducible gene expression and cause an endoplasmic reticulum stress response. DIM alters estrogen metabolism by shifting metabolism from carcinogenic 16α-hydroxy to inert 2-hydroxy derivatives, and it antagonizes estrogen and androgen receptor activity[1].

In vivo Preliminary studies show DIM is most effective against total body irradiation (TBI) when given in multiple once-daily doses. Treatment of DIM has radioprotection and mitigation properties in vivo. DIM Activates ATM in Normal Tissues. DIM can be given to mice (250 mg/kg) by oral gavage, with no acute toxicity and excellent bioavailability[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: 184A1 and Hs578Bst cells
  • Concentrations: 0.3 μM
  • Incubation Time: 24 h
  • Method: The 184A1 and Hs578Bst cells are pretreated with DIM (0.3 μM) or vehicle for 24 h, irradiated by using different doses of 137Cs γ rays, harvested, plated at different densities, incubated for 14 d, and counted for colony formation.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: Sprague-Dawley (SD) rats
  • Dosages: 75 mg/kg
  • Administration: i.p
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 49 mg/mL
(198.93 mM)
Ethanol 18 mg/mL
(73.07 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 246.31
Formula

C17H14N2

CAS No. 1968-05-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C2C(=C1)C(=CN2)CC3=CNC4=CC=CC=C43

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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