SBI-0206965

Catalog No.S7885

For research use only.

SBI-0206965 is a highly selective autophagy kinase ULK1 inhibitor with IC50 of 108 nM, about 7-fold selectivity over ULK2. SBI-0206965 inhibits autophagy and enhances apoptosis in human glioblastoma and lung cancer cells.

SBI-0206965 Chemical Structure

CAS No. 1884220-36-3

Selleck's SBI-0206965 has been cited by 30 publications

Purity & Quality Control

Choose Selective ULK Inhibitors

Biological Activity

Description SBI-0206965 is a highly selective autophagy kinase ULK1 inhibitor with IC50 of 108 nM, about 7-fold selectivity over ULK2. SBI-0206965 inhibits autophagy and enhances apoptosis in human glioblastoma and lung cancer cells.
Targets
ULK1 [1]
(Cell-free assay)
ULK2 [1]
(Cell-free assay)
108 nM 711 nM
In vitro

In HEK293T transfected with WT or KI Myc-tagged ULK1 and WT Vps34, SBI-0206965 inhibits Ser249 phosphorylation of overexpressed Vps34 and Beclin1 Ser15 phosphorylation. SBI-0206965 suppresses autophagy induced by mTOR inhibition in A549 cells, and prevents ULK1-dependent cell survival in WT MEFs. In addition, SBI-0206965 also synergizes with mTOR inhibition to induce cancer cell death. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human Malme-3M cells NUmwZ4l1TnWwY4Tpc44h[XO|YYm= NIrPWIlKdmirYnn0bY9vKG:oIF\MRWcufGGpZ3XkJHVNUzFiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gTGVMOjl|VDDj[YxteyC3c3nu[{BIW1RvbHHi[Yxt\WRiQYTnNVAyKGG|IIP1ZpN1emG2ZTDpckBxemW|ZX7j[UBw\iCpYX3tZU1cOzKfUD3BWHAtUUN3ME2wMlExQCEEtV2u MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJzMUS4NEc,Ojl{MUG0PFA9N2F-
Assay
Methods Test Index PMID
Western blot p-AMPK β1 / AMPK β1 / PARP / cleaved PARP / Caspase 8 / cleaved caspase 8 / LC3B I / LC3B II / p62 ; pACC / ACC / AMPK-pThr-172 30078736 29695504

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 489.32
Formula

C21H21BrN4O5

CAS No. 1884220-36-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CNC(=O)C1=CC=CC=C1OC2=NC(=NC=C2Br)NC3=CC(=C(C(=C3)OC)OC)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy SBI-0206965 | SBI-0206965 supplier | purchase SBI-0206965 | SBI-0206965 cost | SBI-0206965 manufacturer | order SBI-0206965 | SBI-0206965 distributor