RGD (Arg-Gly-Asp) Peptides

Catalog No.S8008

For research use only.

RGD (Arg-Gly-Asp) Peptides is a cell adhesion motif which can mimic cell adhesion proteins and bind to integrins.

RGD (Arg-Gly-Asp) Peptides Chemical Structure

CAS No. 99896-85-2

Selleck's RGD (Arg-Gly-Asp) Peptides has been cited by 13 publications

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Biological Activity

Description RGD (Arg-Gly-Asp) Peptides is a cell adhesion motif which can mimic cell adhesion proteins and bind to integrins.
Targets
Integrin [1]
In vitro

The RGD sequence is the cell attachment site of a large number of adhesive extracellular matrix, blood, and cell surface proteins, and nearly half of the over 20 known integrins recognize this sequence in their adhesion protein ligands. The RGD peptides and mimics can be used to probe integrin functions in various biological systems. Drug design based on the RGD structure may provide new treatments for diseases such as thrombosis, osteoporosis, and cancer. [1] RGD peptide acts as an inhibitor of integrin-ligand interactions and can induce apoptosis in the absence of signals and integrin-mediated cell clustering. Research demonstrates that RGD peptides promote apoptosis through activation of conformation changes that enhance pro-caspase-3 activation and autoprocessing. [2] The RGD peptide can serve as a cell adhesion site of extracellular matrix, cell surface proteins, and integrins. [3] In addition, RGD peptide can inhibit ACK-2 activation through cell adhesion. [4]

Protocol (from reference)

Cell Research:

[5]

  • Cell lines: HLE cell line
  • Concentrations: 0.1, 0.3, 1.0 and 2.0 mg/ml
  • Incubation Time: 90 min
  • Method:

    The cells were seeded at approximately 2.6 × 104 /well on each ECM-coated 24-well plate in the medium containing the monotype of GRGDSP RGD peptide at 0 (control), 0.1, 0.3, 1.0 and 2.0 mg/ml. After 90 min of incubation, the floating cells were removed by washing twice with Ca 2+ -Mg 2+ -free phosphate-buffered saline. Then the attached cells were dissociated with trypsin-EDTA solution. The number of cells attached at each concentration of peptide was counted using a hemocytometer. 

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 346.34
Formula

C12H22N6O6

CAS No. 99896-85-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(CC(C(=O)NCC(=O)NC(CC(=O)O)C(=O)O)N)CN=C(N)N

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