Pepstatin A

Name: Pepstatin A
Brand: Selleck Chemicals
SKU: S7381
Rating: 5 (8 reviews)

For research use only.

Catalog No.S7381 Synonyms: Pepstatin

8 publications

CAS No. 26305-03-3

Pepstatin A (Pepstatin) is a potent aspartic protease inhibitor, and also inhibits HIV replication. Pepstatin A is also an inhibitor of cathepsins D and cathepsins E. Pepstatin A inhibits autophagy by suppressing lysosomal proteases.

Selleck's Pepstatin A has been cited by 8 publications

Purity & Quality Control

Choose Selective HIV Protease Inhibitors

Biological Activity

Description

Targets

aspartic protease ^[1]

In vitro

Pepstatin A shows effective inhibition on aspartic proteinases such as pepsin, cathepsins D and E. In HIV-infected H9 cells, pepstatin A inhibits part of the intracellular processing of the HIV gag protein with no apparent toxicity on HIV-infected cells. ^[1] Pepstatin A laos suppresses the differentiation of osteoclasts by blocking ERK signaling and the inhibition of NFATc1 expression. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
human MCF7 cells	M{XMXGZ2dmO2aX;uJIF{e2G7				NI[ycWxKdmirYnn0bY9vKG:oIHPheIhmeHOrbjDEJIlvKGi3bXHuJG1ETjdiY3XscJMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxRTBwMEC1JO69VQ>?	NXfCeYY5OTl5MUWzNlA>
human MCF7 cells	M4fRW2Z2dmO2aX;uJIF{e2G7		MV:xJIg>		M{TXOWlvcGmkaYTpc44hd2ZiY3H0bIVxe2mwIFSgbY4hcHWvYX6gUWNHPyClZXzsd{B1emWjdHXkJIZweiBzIHjyJI1m[XO3cnXkJIFnfGW{IIfhd4hwfXRiYX7kJJRzgXC\|aX6geJJm[XSvZX70JIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NF02KM7:TR?=	NW\lXoJTOTl5MUWzNlA>

... Click to View More Cell Line Experimental Data

Protocol

References

Solubility (25°C)

In vitro	DMSO	57 mg/mL (83.1 mM)
	Water	Insoluble
	Ethanol	''1 mg/mL warmed

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Pepstatin A SDF

Molecular Weight	685.89
Formula	C₃₄H₆₃N₅O₉
CAS No.	26305-03-3
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	Pepstatin
Smiles	CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
Do you have recommended in-vivo solvent for S7381 please?
Answer:
S7681 could be dissolved in 5%DMSO+40%PEG300+tween-80+ddH2O up to 2mg/ml.

HIV Protease Signaling Pathway Map

Related HIV Protease Products

Calpeptin ^New

Calpeptin is a potent, cell-permeable calpain inhibitor with ID50 of 52 nM, 34 nM, 138 nM, and 40 nM for Calpain I (porcine erythrocytes), Calpain II (porcine kidney), Papainb, and Calpain I (human platelets), respectively. Calpeptin attenuates apoptosis and intracellular inflammatory changes in muscle cells.
Marimastat (BB-2516) ^New

Marimastat (BB-2516) is a broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-9, MMP-1, MMP-2, MMP-14 and MMP-7 with IC50 of 3 nM, 5 nM, 6 nM, 9 nM and 13 nM, respectively. Phase 3.
Ledipasvir (GS5885) ^New

Ledipasvir (GS5885) is a HCV NS5A polymerase inhibitor, used for the treatment of hepatitis C virus infection.
Ritonavir

Ritonavir (ABT-538, A 84538) is a Cytochrome P450 3A and Protease Inhibitor; Also inhibits Cytochrome P450 2D6, P-Glycoprotein and induces Cytochrome P450 2C19, Cytochrome P450 1A2, Cytochrome P450 2C9, Cytochrome P450 2B6 and UDP Glucuronosyltransferases. Ritonavir induces apoptosis.
Lopinavir

Lopinavir (ABT-378) is a potent HIV protease inhibitor with K_i of 1.3 pM in a cell-free assay.
Darunavir Ethanolate

Darunavir Ethanolate (TMC-114, UIC 94017) is a nonpeptidic HIV protease inhibitor, used to treat HIV infection.
Amprenavir

Amprenavir (141W94, VX-478, KVX-478) is a potent PXR-selective agonist, and an HIV protease inhibitor, used to treat HIV.
Limonin

Limonin is a triterpenoid enriched in citrus fruits, which has antivirus and antitumor ability.
Atazanavir Sulfate

Atazanavir Sulfate (BMS-232632) is a HIV protease inhibitor with K_i of 2.66 nM in a cell-free assay.

Features:Atazanavir is generally more potent than other HIV-1 Prt inhibitors, including IDV, SQV, RTV, NFV, and APV.
Nelfinavir Mesylate

Nelfinavir Mesylate (Viracept, AG1343) is a potent HIV protease inhibitor with K_i of 2 nM.

Choose Your Country or Region

By Signaling Pathways

By product type

Pepstatin A