For research use only.

Catalog No.S8228

1 publication

NT157 Chemical Structure

Molecular Weight(MW): 412.26

NT157, a selective inhibitor of IRS-1/2(insulin receptor substrate), has the potential to inhibit IGF-1R and STAT3 signaling pathways in cancer cells and stroma cells of TME leading to a decrease in cancer cell survival.

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Selleck's NT157 has been cited by 1 publication

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Biological Activity

Description NT157, a selective inhibitor of IRS-1/2(insulin receptor substrate), has the potential to inhibit IGF-1R and STAT3 signaling pathways in cancer cells and stroma cells of TME leading to a decrease in cancer cell survival.
IRS1/2 [2]
In vitro

NT157 treatment resulted in dose-dependent inhibition of IGF1R activation, suppression of IRS protein expression, inhibition of IGF1-induced AKT activation, but increased ERK activation in NT157-treated cells in vitro. These effects were correlated with decreased proliferation and increasing apoptosis of LNCaP cells and increasing G2-M arrest in PC3 cells. NT157 can mediate suppression of IGF1R-mediated survival signaling through the established mechanism for negative feedback of IGF1R signaling: targeting IRS1/2 for serine phosphorylation and subsequent degradation[1]. NT157 displayed little to no effect on the survival of normal melanocytes and fibroblasts[2].

Methods Test Index PMID
Western blot
IRS-1 / IRS-2 ; 

PubMed: 26029165     

Inhibition of cell motility is mediated by downregulation of IRS-2 and IRS-1 in MG-63 and U-2OS cells after 24 h of treatment with NT157 (1–3 μM). β-actin was used as loading control.

p-IRS1 / p-AKT / AKT / p-S6 / S6 / Shc / p-ERK / ERK ; 

PubMed: 26029165     

Analysis of major downstream signaling of IRS-1 after treatment with or without NT157 (1-3 μM) by western blotting using 40 μg of total protein cell lysate. GAPDH was used as a loading control. The figure shows data representative of two independent experiments.

Growth inhibition assay
Cell viability; 

PubMed: 26029165     

The in vitro sensitivity to a selected inhibitor of IRS-1/2, NT157, for a panel of OS cell lines. Cell growth was assessed by staining cells with Trypan Blue and counting viable cells after up to 72 h of exposure to NT157 (0.3-3 μM) in MG-63, OS-19, and U-2OS cells. Points indicate three independent experiments; barsindicate the SE.

In vivo NT157 suppressed androgen-responsive growth, delayed CRPC progression of LNCaP xenografts, and suppressed PC3 tumor growth alone and in combination with docetaxel[1]. Melanoma tumor growth and metastasis is efficiently inhibited by NT157[2].


Cell Research:[1]
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  • Cell lines: LNCaP cells, PC3 cells
  • Concentrations: 0-10 μM
  • Incubation Time: 72 h
  • Method: Cells were plated in 24-well plates and treated with varying doses of NT157. Crystal violet staining was carried out for time course, and 72 hours after treatment. The absorbance was determined with a microtiter plate reader at 562 nm. Cell survival after NT157 treatment was calculated as the percentage of the absorbance in vehicle-treated cells.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Athymic nude mice (Harlan Sprague-Dawley)
  • Dosages: 50 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 82 mg/mL (198.9 mM)
Ethanol 82 mg/mL (198.9 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 412.26


CAS No. 1384426-12-3
Storage powder
in solvent
Synonyms N/A
Smiles OC1=CC(=CC(=C1O)O)CNC(=S)/C=C/C2=CC(=C(O)C(=C2)Br)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID