Catalog No.S8439

Monastrol Chemical Structure

Molecular Weight(MW): 292.35

Monastrol is a cell-permeable small molecule inhibitor of kinesin-5(KIF11) which is essential for maintaining separation of the half-spindles.

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Biological Activity

Description Monastrol is a cell-permeable small molecule inhibitor of kinesin-5(KIF11) which is essential for maintaining separation of the half-spindles.
KIF11(Eg5) [4]
(Cell-based assay)
14 μM
In vitro

Monastrol does not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol is also rapidly reversible. Monastrol also inhibits bipolar spindle formation in Xenopus egg extracts. Monastrol arrests cells in mitosis with monoastral spindles comprised of a radial array of microtubules surrounded by a ring of chromosomes while it does not affect microtubules in interphase cells or microtubule polymerization in vitro[1]. Exposure of cultured sympathetic neurons to monastrol for a few hours increases both the number and the growth rate of the axons. With additional time, the overall lengths of the axons are indistinguishable from controls. Sensory neurons shows a similar short-term increase in axonal growth rate. However, prolonged exposure results in shorter axons, suggesting that sensory neurons may be more sensitive to toxic effects of the drug. Nevertheless, the overall health of the cultures is still far more robust than cultures treated with taxol, a drug commonly used for anti-cancer therapy[2]. In HeLa cells, monastrol activates the spindle checkpoint, leading to mitotic arrest and apoptosis[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 cells NUXiT5VUTnWwY4Tpc44h[XO|YYm= MVfF[oZm[3Rib36gZ4VtdCCleXPs[UBxem:pcnXzd4lwdiCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMhdWm2b4TpZ{BienKnc4SgcYVie3W{ZXSgZpkh\G:3YnzpcochTE6DIHPvcpRmdnRiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHluIFXDOVA:OS5{IN88US=> NWGyfnVHOTh5OUO4OFc>
HeLa cells MnW2SpVv[3Srb36gZZN{[Xl? MmLLNVIhcA>? NX\a[2F5UW6qaXLpeIlwdiCxZjDF[|UhSVSSYYPlJIFkfGm4aYT5JIV5eHKnc4Pl[EBqdiCKZVzhJINmdGy|IHHmeIVzKDF{IHjyd{whUUN3ME22MlEh|ryP NH3rXJAyPzV6N{W4Oi=>
M14 cells M{joSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF\rNndIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOOTRiY3XscJMtKEeLNUC9NlUvOSEQvF2= NXjKdpczOjF6NUWzOVE>
HL-60(TB) cells NUW5bpU5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\BNVVNT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUExvNkCoWGIqKGOnbHzzMEBIUTVyPUK1MlEh|ryP NWLUb2xSOjF6NUWzOVE>
KM12 cells M3q1NWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NW[yeVdET3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hU01zMjDj[YxteyxiR1m1NF0{OS54IN88US=> MXyyNVg2PTN3MR?=
SF295 cells MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV;Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTSlI6PSClZXzsd{whT0l3ME2zNU43KM7:TR?= M{HtdFIyQDV3M{Wx
SR cells MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkjpS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV3Ih[2WubIOsJGdKPTB;M{GuOkDPxE1? MlfBNlE5PTV|NUG=
MOLT4 cells M{DFVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNU2xVPCClZXzsd{whT0l3ME2zNU43KM7:TR?= NG\R[|UzOTh3NUO1NS=>
NCI-H522 cells NYnzTIt5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDOR2kuUDV{MjDj[YxteyxiR1m1NF0{OS54IN88US=> M32yeVIyQDV3M{Wx
K562 cells MlHUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDLOVYzKGOnbHzzMEBIUTVyPUOxMlYh|ryP MmDlNlE5PTV|NUG=
CCRF-CEM cells NYj6UJZpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn3jS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gR2NTTi2FRV2gZ4VtdHNuIFfJOVA:OzFwNjFOwG0> MYSyNVg2PTN3MR?=
SW620 cells MmDHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTW|YzOCClZXzsd{whT0l3ME2zPU45KM7:TR?= MX2yNVg2PTN3MR?=
SK-MEL-5 cells NHS4S3JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mo\5S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2suVUWOLUWgZ4VtdHNuIFfJOVA:OzlwODFOwG0> M1e2UFIyQDV3M{Wx
UACC62 cells NWTvbFNmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXnW[ng2T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQ{[yJINmdGy|LDDHTVUxRTN7Lkig{txO MnPxNlE5PTV|NUG=
HCT15 cells MkjmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFPzfndIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS1RzNTDj[YxteyxiR1m1NF0{QS56IN88US=> NFfW[JkzOTh3NUO1NS=>
NCI-H322M cells NHT1NXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVq0UFhwT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVizNlJOKGOnbHzzMEBIUTVyPUO5Mlgh|ryP MUOyNVg2PTN3MR?=
HCC2998 cells MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDIR2MzQTl6IHPlcIx{NCCJSUWwQVM6NjhizszN MWOyNVg2PTN3MR?=
hTERT-HME1 cells MlG1VJJwdGmoZYLheIlwdiCjc4PhfS=> M1m5SVczKGh? MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGiWRWLUMWhOTTFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGFt[W2jcjDicJVmKGG|c3H5MEBGSzVyPUS1MlA5OiEQvF2= NHHlPGMzODV7N{S4OS=>
KBV1 cells NYj4VGtNWHKxbHnm[ZJifGmxbjDhd5NigQ>? M4rpZVczKGh? NI\kOVdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFvCWlEh[2WubIOgc5ZmemW6cILld5NqdmdiTVTSNUBi\nSncjC3NkBpenNiYomgRYxidWG{IHLseYUh[XO|YYmgbY4heHKnc3XuZ4Uhd2Zien;zeZF2cWSjcjygSWM2OD12NT6zPVQh|ryP MW[yNFU6PzR6NR?=

... Click to View More Cell Line Experimental Data


Cell Research:


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  • Cell lines: BS-C-1 (monkey epithelial kidney) cells
  • Concentrations: 100 μM
  • Incubation Time: 4 h
  • Method:

    For the double thymidine arrest, exponentially growing BS-C-1 cells are cultured for 16 h in normal growth medium containing 2 mM thymidine. After this, the cells are released into normal growth medium supplemented with 24 μM deoxycytidine for 9 h. The second thymidine block is imposed for 16 h during which the cells were maintained in serum-free medium containing 2 mM thymidine. Finally, the cells are released into normal growth medium containing 24 μM deoxycytidine to which is added either 100 μM monastrol or 0.1% DMSO. To assess the reversibility of the effect of monastrol and nocodazole treatment, BS-C-1 cells plated on coverslips are treated for 4 h in normal growth medium containing either 2 μM nocodazole or 100 μM monastrol and then released into normal medium. At the different time points, coverslips are processed for immunofluorescence and the cells in interphase or mitosis are counted and categorized.

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (198.39 mM)
Ethanol 58 mg/mL (198.39 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 292.35


CAS No. 329689-23-8
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID