For research use only.

Catalog No.S8439

3 publications

Monastrol Chemical Structure

Molecular Weight(MW): 292.35

Monastrol is a cell-permeable small molecule inhibitor of kinesin-5(KIF11) which is essential for maintaining separation of the half-spindles.

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Biological Activity

Description Monastrol is a cell-permeable small molecule inhibitor of kinesin-5(KIF11) which is essential for maintaining separation of the half-spindles.
KIF11(Eg5) [4]
(Cell-based assay)
14 μM
In vitro

Monastrol does not inhibit progression through S and G2 phases of the cell cycle or centrosome duplication. The mitotic arrest due to monastrol is also rapidly reversible. Monastrol also inhibits bipolar spindle formation in Xenopus egg extracts. Monastrol arrests cells in mitosis with monoastral spindles comprised of a radial array of microtubules surrounded by a ring of chromosomes while it does not affect microtubules in interphase cells or microtubule polymerization in vitro[1]. Exposure of cultured sympathetic neurons to monastrol for a few hours increases both the number and the growth rate of the axons. With additional time, the overall lengths of the axons are indistinguishable from controls. Sensory neurons shows a similar short-term increase in axonal growth rate. However, prolonged exposure results in shorter axons, suggesting that sensory neurons may be more sensitive to toxic effects of the drug. Nevertheless, the overall health of the cultures is still far more robust than cultures treated with taxol, a drug commonly used for anti-cancer therapy[2]. In HeLa cells, monastrol activates the spindle checkpoint, leading to mitotic arrest and apoptosis[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 cells MnrCSpVv[3Srb36gZZN{[Xl? NHnKVoNG\m[nY4Sgc44h[2WubDDjfYNt\SCycn;ndoV{e2mxbjDpckBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNibXn0c5Rq[yCjcoLld5QhdWWjc4Xy[YQh[nliZH;1ZoxqdmdiRF7BJINwdnSnboSgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImsJGVEPTB;MT6yJO69VQ>? MkX0NVg4QTN6NEe=
HeLa cells NEDueFNHfW6ldHnvckBie3OjeR?= Ml31NVIhcA>? NGrYRnFKdmirYnn0bY9vKG:oIFXnOUBCXFCjc3WgZYN1cX[rdImg[ZhxemW|c3XkJIlvKEinTHGgZ4VtdHNiYX\0[ZIhOTJiaILzMEBKSzVyPU[uNUDPxE1? NXSxXYZUOTd3OEe1PFY>
M14 cells M3:4dGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mo\yS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUVE1KGOnbHzzMEBIUTVyPUK1MlEh|ryP MXOyNVg2PTN3MR?=
HL-60(TB) cells NWizVm5{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MonWS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGwuPjBqVFKpJINmdGy|LDDHTVUxRTJ3LkGg{txO MUWyNVg2PTN3MR?=
KM12 cells MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkS5S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gT20yOiClZXzsd{whT0l3ME2zNU43KM7:TR?= Ml76NlE5PTV|NUG=
SF295 cells NEK0VFFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoDJS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV2YzQTViY3XscJMtKEeLNUC9N|EvPiEQvF2= NIDUSXMzOTh3NUO1NS=>
SR cells NF3kPVZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoXGS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV3Ih[2WubIOsJGdKPTB;M{GuOkDPxE1? MXyyNVg2PTN3MR?=
MOLT4 cells MofMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNU2xVPCClZXzsd{whT0l3ME2zNU43KM7:TR?= NXGy[|NUOjF6NUWzOVE>
NCI-H522 cells M3n1TGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1LySGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KNUKyJINmdGy|LDDHTVUxRTNzLk[g{txO MUOyNVg2PTN3MR?=
K562 cells MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEPG[|JIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBMPTZ{IHPlcIx{NCCJSUWwQVMyNjZizszN MkDFNlE5PTV|NUG=
CCRF-CEM cells Mon4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEe5dW9Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBES1KILVPFUUBk\WyuczygS2k2OD1|MT62JO69VQ>? NXXlUHRbOjF6NUWzOVE>
SW620 cells NYq1WmJvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mk\US5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV3c3OjBiY3XscJMtKEeLNUC9N|kvQCEQvF2= NVPqfm1XOjF6NUWzOVE>
SK-MEL-5 cells M3zsSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYLHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTT{1OTUxvNTDj[YxteyxiR1m1NF0{QS56IN88US=> NUPMNHdPOjF6NUWzOVE>
UACC62 cells NVPFeHVPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUf4SHRzT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQ{[yJINmdGy|LDDHTVUxRTN7Lkig{txO NYnEV|lHOjF6NUWzOVE>
HCT15 cells NUnjNppJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEPvd|JIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS1RzNTDj[YxteyxiR1m1NF0{QS56IN88US=> NGLwXXEzOTh3NUO1NS=>
NCI-H322M cells Ml60S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoOyS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUmNKNUh|MkLNJINmdGy|LDDHTVUxRTN7Lkig{txO MVSyNVg2PTN3MR?=
HCC2998 cells M3vQU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFj5N|JIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS0N{OUm4JINmdGy|LDDHTVUxRTN7Lkig{txO NV7HSGl3OjF6NUWzOVE>
hTERT-HME1 cells MmDHVJJwdGmoZYLheIlwdiCjc4PhfS=> MXy3NkBp NHHXXoxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIHjUSXJVNUiPRUGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KEGuYX3hdkBjdHWnIHHzd4F6NCCHQ{WwQVQ2NjB6MjFOwG0> Ml\oNlA2QTd2OEW=
KBV1 cells NUf3VVlCWHKxbHnm[ZJifGmxbjDhd5NigQ>? NYX4O5Z7PzJiaB?= M1;KU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iS1LWNUBk\WyuczDveoVz\XiycnXzd4lv\yCPRGKxJIFnfGW{IEeyJIhzeyCkeTDBcIFu[XJiYnz1[UBie3OjeTDpckBxemW|ZX7j[UBw\iC8b4P1dZVq\GG{LDDFR|UxRTR3LkO5OEDPxE1? M{n5S|IxPTl5NEi1

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 26035434     

Cells from AGS, HepG2, Lovo39, Du145, and HT29 cell lines, indicated at the lower left corner of each panel, were incubated for up to three days with various concentrations of monastrol, indicated on the right (0, 20, 50, 100, and 150μM). The number of viable cells (% of control) was determined by XTT assay for mitochondrial activity. Points and bars represent an average and SEM of 3–4 independent experiments. *P<0.05: the number of viable Du145 cells following 2-day treatment with 20 or 50μM monastrol, compared to viable HT29 cells identically treated (red and blue circles). The results indicate that the different cell lines are differently sensitive to monastrol, with the sensitivity ranking: AGS>HepG2>Lovo>Du145≥HT29.

Western blot
Cyclin B / Survivin ; 

PubMed: 26035434     

Representative WB analysis of cyclin B and survivin protein expression in AGS (left) and HT29 (right) cells treated with monastrol for 12h and 24h, indicated on the bottom. 



Cell Research:


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  • Cell lines: BS-C-1 (monkey epithelial kidney) cells
  • Concentrations: 100 μM
  • Incubation Time: 4 h
  • Method:

    For the double thymidine arrest, exponentially growing BS-C-1 cells are cultured for 16 h in normal growth medium containing 2 mM thymidine. After this, the cells are released into normal growth medium supplemented with 24 μM deoxycytidine for 9 h. The second thymidine block is imposed for 16 h during which the cells were maintained in serum-free medium containing 2 mM thymidine. Finally, the cells are released into normal growth medium containing 24 μM deoxycytidine to which is added either 100 μM monastrol or 0.1% DMSO. To assess the reversibility of the effect of monastrol and nocodazole treatment, BS-C-1 cells plated on coverslips are treated for 4 h in normal growth medium containing either 2 μM nocodazole or 100 μM monastrol and then released into normal medium. At the different time points, coverslips are processed for immunofluorescence and the cells in interphase or mitosis are counted and categorized.

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (198.39 mM)
Ethanol 58 mg/mL (198.39 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 292.35


CAS No. 329689-23-8
Storage powder
in solvent
Synonyms N/A
Smiles CCOC(=O)C1=C(C)NC(=S)NC1C2=CC(=CC=C2)O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID