L-NAME HCl

For research use only.

Catalog No.S2877

19 publications

L-NAME HCl Chemical Structure

CAS No. 51298-62-5

L-NAME is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively.

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Selleck's L-NAME HCl has been cited by 19 publications

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Choose Selective NOS Inhibitors

Biological Activity

Description L-NAME is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively.
Targets
nNOS [1]
(Cell-free assay)
eNOS [1]
(Cell-free assay)
15 nM(Ki) 39 nM(Ki)
In vitro

NG-nitro-L-arginine methyl ester (L-NAME; at 0.1-100 mM) causes concentration-dependent inhibition of the Ca2(+)-dependent endothelial NO synthase from porcine aortae. L-NAME causes an endothelium-dependent contraction and an inhibition of the endothelium-dependent relaxation induced by acetylcholine (ACh) in aortic rings. [2] In another research, Viability of rMC-1 cells or BREC in 25 mM glucose is significantly less than at 5 mM glucose, and this cell death is inhibited by l-NAME in both cell types. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BV2 cells MXPGeY5kfGmxbjDhd5NigQ>? NFXRWlIzPCCq NE\JdHhKdmirYnn0bY9vKG:oIF7peJJq[yCxeHnk[UB{gW62aHHz[UBi[3Srdnn0fUBqdiCvb4Xz[UBDXjJiY3XscJMh[XO|ZYPz[YQh[XNiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCjZoTldkAzPCCqcoOgZpkhT3KrZYPzJJJm[WO2aX;uMEBKSzVyPUG4Mlkh|ryP MYeyNVM4PzN4OB?=
mouse RAW264.7 cells NFHxW4NHfW6ldHnvckBie3OjeR?= NWj0b|N5OTdvMkCgbC=> MV7BcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhUU[QLXfhcY1iN0ySUz3zeIlufWyjdHXkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIHHmeIVzKDF5IITvJFIxKGi{czDifUBIemmnc4OgZZN{[XluIFnDOVA:OjdwMUOg{txO MlfyNVk{PTlyNki=

... Click to View More Cell Line Experimental Data

In vivo L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2]

Protocol

Kinase Assay:[1]
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Enzyme Assay:

The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated.
Cell Research:[3]
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  • Cell lines: rMC-1 cells
  • Concentrations: 1 mM
  • Incubation Time: 5 days
  • Method: rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Male Wistar rats
  • Dosages: 100 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro Water 54 mg/mL (200.22 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 269.69
Formula

C7H15N5O4.HCl

CAS No. 51298-62-5
Storage powder
in solvent
Synonyms N/A
Smiles COC(=O)C(CCCN=C(N)N[N+](=O)[O-])N.Cl

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID