Isatin

Catalog No.S4717 Synonyms: 2,3-Indolinedione

Isatin Chemical Structure

Molecular Weight(MW): 147.13

Isatin is an endogenous MAO inhibitor with an IC50 of 15 μM and more active against MAO-B than MAO-A.

Size Price Stock Quantity  
USD 97 In stock
USD 297 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective MAO Inhibitors

Biological Activity

Description Isatin is an endogenous MAO inhibitor with an IC50 of 15 μM and more active against MAO-B than MAO-A.
Targets
MAO-B [1]
(Cell-free assay)
MAO [1]
(Cell-free assay)
MAO-A [1]
(Cell-free assay)
14 μM 15 μM 58 μM
In vitro

Isatin induces apoptosis of MCF-7 cells. Bcl-2 expression is decreased and the ratio of Bcl-2 to Bax is significantly decreased by isatin. The mitochondrial transmembrane potential is markedly decreased and the release of cytochrome c into the cytosol is elevated following treatment with isatin. At the same time, caspase-9 and -3 are stimulated, followed by the degradation of ICAD, a caspase-3 substrate. Isatin and its analogs inhibits the proliferation of some cancer cells, including colon HT29, breast MCF-7, lung A549 and melanoma UACC903 cells and is a dual inhibitor of tubulin polymerization and the Akt pathway[4].

In vivo Isatin is an endogenous indole that is increased in stress, inhibits monoamine oxidase (MAO) B and improves bradykinesia and striatal dopamine levels in rat models of Parkinson's disease. Isatin has a distinct and discontinuous distribution in rat brain and other tissues; the highest concentrations in the brain are found in the hippocampus and cerebellum. In rodent models isatin has been shown to cause a widespectrum of dose-dependent physiological and biological actions, such as anxiogenic and sedative effects, memory dysfunction and inhibition of food and water intake. Significantly, isatin readily crosses the blood-brain barrier so that a peritoneal dose of 100 mg/kg would result in a concentration of about 120 μM in the rat brain. This concentration would increase further with repeated injections[2].

Protocol

Cell Research:[2]
+ Expand
  • Cell lines: human neuroblastoma (SH-SY5Y) cells
  • Concentrations: 0.1-400 μM
  • Incubation Time: 24 or 48 h
  • Method: Cell viability was estimated by a colorimetric method, which is based on the ability of cellular dehydrogenases of viable cells to reduce MTT from a yellow watersoluble dye to a dark blue insoluble formazan product. SHSY5Y cells were seeded in 96-well plates at 4×104 cells/well/(100 ml) and allowed to attach. The cells were then treated with isatin and returned to the incubator for 24 or 48 h. MTT 25 μl (5 mg/ml) was added to all wells and allowed to incubate in the dark at 37℃ for 2 h followed by cell lysis. The plates were read with an OPTImax microplate reader at wavelength of 562 nm. Controls included untreated cells and medium alone, with all MTT assays performed in triplicate.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Albino rats of the Fisher strain
  • Formulation: DMSO
  • Dosages: 100 mg/kg
  • Administration: i.p
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 29 mg/mL (197.1 mM)
Ethanol 10 mg/mL (67.96 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 147.13
Formula

C8H5NO2

CAS No. 91-56-5
Storage powder
in solvent
Synonyms 2,3-Indolinedione

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

MAO Signaling Pathway Map

Related MAO Products

Tags: buy Isatin | Isatin supplier | purchase Isatin | Isatin cost | Isatin manufacturer | order Isatin | Isatin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID