Catalog No.S1883 Synonyms: NSC 39661, SKF 14287

For research use only.

Idoxuridine (NSC 39661, SKF 14287) is an antiviral agent for feline herpesvirus type-1 with IC50 of 4.3 μM.

Idoxuridine Chemical Structure

CAS No. 54-42-2

Purity & Quality Control

Choose Selective Antiviral Inhibitors

Biological Activity

Description Idoxuridine (NSC 39661, SKF 14287) is an antiviral agent for feline herpesvirus type-1 with IC50 of 4.3 μM.
Feline herpesvirus type-1(FHV-1) [1]
4.3 μM
In vitro

Idoxuridine is a nucleoside analogue, a modified form of deoxyuridine, similar enough to be incorporated into viral DNA replication, but the iodine atom added to the uracil component blocks base pairing.Idoxuridine is used for topical treatment of herpetic eye infections due to herpes simplex virus (HSV)[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: Cultured Crandell-Reese feline kidney (CRFK) cells and FHV-1 strain 727.
  • Concentrations: 5, 10, and 50 µM
  • Incubation Time: 24, 48, and 72 h
  • Method: The CRFK cells are cultured in 25-cm2 flasks that contained DMEM with 10% FBS. The concentration of idoxuridine is 5, 10, and 50µM. Cells from 1 control flask and 1 flask containing each drug concentration are examined by use of an inverted microscope to detect morphologic changes and evaluate confluence, and cells are then harvested at 24, 48, and 72 hours. Culture medium is decanted, and the monolayer is rinsed once with PBS solution. Cells are then enzymatically detached with trypsin-EDTA solution. Trypsinization is stopped by the addition of DMEM with 10% FBS, and cells are forcefully pipetted to ensure complete detachment from the flasks. Each flask is then rinsed once with DMEM with 10% FBS, and the eluent is combined with the first cell suspension to ensure collection of as many cells as possible from each flask. Cell-containing medium is centrifuged (300 X g for 6 minutes), and the supernatant is decanted. Cells are then resuspended in a known volume of DMEM and counted on a hemacytometer. Cells cultured in each drug concentration at each time point are counted twice, and the total number of cells in each flask is calculated.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 354.1


CAS No. 54-42-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(OC1N2C=C(C(=O)NC2=O)I)CO)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Idoxuridine | Idoxuridine ic50 | Idoxuridine price | Idoxuridine cost | Idoxuridine solubility dmso | Idoxuridine purchase | Idoxuridine manufacturer | Idoxuridine research buy | Idoxuridine order | Idoxuridine mouse | Idoxuridine chemical structure | Idoxuridine mw | Idoxuridine molecular weight | Idoxuridine datasheet | Idoxuridine supplier | Idoxuridine in vitro | Idoxuridine cell line | Idoxuridine concentration | Idoxuridine nmr