Histamine

Catalog No.S3968

For research use only.

Histamine, an organic nitrogenous compound, is involved in local immune responses regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is a potent H1 and H2 receptor agonist.

Histamine Chemical Structure

CAS No. 51-45-6

Selleck's Histamine has been cited by 1 Publication

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Biological Activity

Description Histamine, an organic nitrogenous compound, is involved in local immune responses regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is a potent H1 and H2 receptor agonist.
In vitro

Histamine stimulates the proliferation of human articular chondrocytes in culture. Histamine is reported to modify the behaviour of many cell types in vitro, including chondrocytes, fibroblasts, macrophages, epithelial cells, endothelial cells, and T cells. Histamine also modulates the production of many cytokines and the expression of their receptors. Histamine regulates cellular processes through the expression of histamine receptors. Histamine stimulates the production of matrix metalloproteinases (MMPs)-13 and -3 (collagenase 3 and stromelysin-1, respectively) by histidine decarboxylase (HAC) in vitro[1].

In vivo Histamine has a recognised role in allergic and inflammatory reactions and is an important modulator of numerous physiological processes, including cell proliferation, angiogenesis, and vasopermeability[1]. Histamine dilates vasculature, increases blood flow, while it induces hyperpermeability in venula. It disrupts endothelial barrier formation of venula indicated by changes in vascular endothelial cadherin (VE-cadherin) localization at endothelial cell junction[3].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: human articular chondrocytes
  • Concentrations: 1-100 μmol/l
  • Incubation Time: 6 days
  • Method: Cells are grown to confluence in 80 cm2 culture flasks in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal calf serum (FCS). At first or second passage, cells are seeded into 96 well culture plates, at densities of approximately 2×103 cells/well. After 24 hours the cells are treated with either DMEM +2% FCS alone (control), or DMEM + 2% FCS plus histamine range 1-100 μmol/l (minimum of eight wells for each treatment). The medium with or without histamine is replenished every 48 hours, and after six days cell growth is estimated.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: New Zealand adult healthy albino rabbits
  • Dosages: 50, 100, 200 μg/kg
  • Administration: s.c.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 22 mg/mL
(197.93 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 111.15
Formula

C5H9N3

CAS No. 51-45-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=C(NC=N1)CCN

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04828317 Not yet recruiting Other: Pork Challenge Allergy;Food University of Virginia May 1 2021 Not Applicable
NCT04699604 Recruiting Drug: Levocetirizine Dihydrochloride|Drug: Placebo Allergic Asthma Children''s Mercy Hospital Kansas City April 28 2021 Phase 3
NCT04913623 Recruiting Procedure: Hip Replacement Arthroplasty Complications University Clinical Centre of Kosova March 23 2021 --
NCT04534036 Recruiting Dietary Supplement: PDS-08|Other: Placebo Constipation|Signs and Symptoms|Digestive Signs and Symptoms|Infrequent or Difficult Evacuation Seed Health|Curebase Inc. August 13 2020 Not Applicable
NCT05022108 Completed Biological: Alpha bisabolol gel Wounds and Injuries Universidade do Vale do Sapucai April 1 2020 Phase 1
NCT04439955 Recruiting Drug: CBD Chronic Spontaneous Urticaria Stero Biotechs Ltd. February 1 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2021-09-06)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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