For research use only.
Molecular Weight(MW): 184.07
Histamine is an organic nitrogen compound, acts on target cells in mammalian brain via stimulation of Histamine 1/2.
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Choose Selective Histamine Receptor Inhibitors
|Description||Histamine is an organic nitrogen compound, acts on target cells in mammalian brain via stimulation of Histamine 1/2.|
Histamine suppresses the generation of ROS through the Histaminetype-2 receptor (H2 receptor). Histamine inhibits the generation and release of reactive oxygen species (ROS) by monocytes/macrophages (MO) during respiratory burst. Histamine and interleukin-2 (IL-2) act synergistically to activate NK cell cytotoxicity (NKCC). Histamine combined with IL-2 might improve response rates and disease-free survival by protecting the cells of the immune system from oxidative stress and inducing natural endogenous immune cytotoxicity. 
|In vivo||Histamine treatment (0.5 mg/kg or 5.0 mg/kg, twice daily) protects against liver injury as evident by normal serum transaminase levels and significantly reduced liver pathology scores in a rat model with early alcohol-induced liver injury. The protective effect of histamine is blocked by Ranitidine (10 mg/kg), an H2 receptor antagonist, indicating that the histamine effect is predominantly mediated through the H2 receptor.  Histamine (30 pg/rat, icv) increases both 3,4-dihydroxyphenylalanine accumulation and 3,4-dihydroxyphenylalanine acid concentrations in the nucleus accumbens in male rats, and this effect is not affect by H2 antagonist zolantidine, indicating that histamine stimulates mesolimbic DA neurons through an action at the H1 receptor.  Histamine (0.5 mg/kg s.c.) reduces the liver tumour weight by 46% and subcutaneous tumour weight by 41% versus rats receiving subcutaneous saline injections. The anti-tumour effect observed by subcutaneous histamine injections is inhibited by Ranitidine (50 mg/kg s.c.) in rats sarcoma.  Histamine (1000 mg/kg s.c.) displays acute tissue damage after 24 hours and indications of pathological inflammation at the injection sites at 5 days and 28 days in Sprague-Dawley rats. Histamine (1000 mg/kg s.c.) results in Cmax of 167 mM, tmax of 0.5 hour, t1/2 of 0.95 and AUC of 186 mmol-h/L in male Sprague-Dawley rats. |
-  Hornyak SC, et al. Inflammation, 2003, 27(5), 317-327.
-  Agarwala SS, et al. Expert Opin Biol Ther, 2001, 1(5), 869-879.
-  Fleckenstein AE, et al. Naunyn Schmiedebergs Arch Pharmacol, 1993, 347(1), 50-54.
|In vitro||Water||37 mg/mL (201.01 mM)|
|DMSO||3 mg/mL (16.29 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04286542||Recruiting||Diagnostic Test: Cold dry air||Hyperactivity|Allergic Rhinitis Due to House Dust Mite|Chronic Rhinosinusitis With Nasal Polyps||Universitaire Ziekenhuizen Leuven||January 20 2020||Not Applicable|
|NCT03930537||Completed||Device: Hip replacement||Arthroplasty Complications||University Clinical Centre of Kosova||March 17 2019||Not Applicable|
|NCT03826004||Completed||Drug: Clemastine|Drug: Saline Solution||Efficacy and Safety||Chinese Academy of Medical Sciences Fuwai Hospital||February 20 2019||Not Applicable|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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