Name: Folic acid
Brand: Selleck Chemicals
SKU: S4605
Availability: InStock
Rating: 5 (3 reviews)

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Quality Control

Batch: Purity: 99.93%

99.93

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase
Other Vitamin Inhibitors	Trolox Nicotinic Acid Sodium ascorbate D-Pantethine Amprolium HCl Thiamine pyrophosphate hydrochloride D-Pantothenate Sodium Calcium D-Panthotenate Tocofersolan Ergosterol

Solubility

In vitro
Batch:

DMSO : 2 mg/mL (4.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.2mg/ml (4.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 44 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.31mg/ml (0.70mM)	Taking the 1 mL working solution as an example, add 50 μL of 6.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	441.40	Formula	C₁₉H₁₉N₇O₆	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	59-30-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate			SMILES	C1=CC(=CC=C1C(=O)NC(CCC(=O)O)C(=O)O)NCC2=CN=C3C(=N2)C(=O)NC(=N3)N

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro

Folic acid treatment induces dose-related increases in BRCA1 mRNA expression in HepG2, Huh-7D12, Hs578T, and JURKAT and in BRCA2 in HepG2, Hs578T, MCF7, and MDA-MB-157 cells. This compound does not affect the corresponding normal cells or on any of the ovarian cell lines. It induces increased BRCA1 protein expression in Hs578T, but not HepG2 cells, whereas BRCA2 protein levels are undetectable. FA treatment does not alter DNA repair in liver-derived cells, whereas there are transient effects on breast-derived cells. There is no effect of this treatment on BRCA1 or BRCA2 DNA methylation, although there is some variation in the methylation of specific CpG loci between some cell lines.

References

[1] https://pubmed.ncbi.nlm.nih.gov/25960189/
[2] https://pubmed.ncbi.nlm.nih.gov/10539786/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-01-14)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07113158	NOT_YET_RECRUITING	Sudden Sensorineural Hearing Loss	Assistance Publique - Hôpitaux de Paris	2026-04-01	PHASE3
NCT06641245	NOT_YET_RECRUITING	Folate; Folate Bioavailability; Clinical Trials; Pregnancy	University of British Columbia	2025-01-31
NCT07241221	RECRUITING	Perinatal and Neonatal Outcomes in Pregnancies With Type 2 Diabetes Mellitus (T2DM)	National University Hospital, Singapore	2025-12
NCT07695883	NOT_YET_RECRUITING	Neurodevelopment Outcome; Linear Growth; Micronutrient Status; Bone Mineral Density; Underweight; Stunting; Body Composition; Blood Pressure; HRQOL (Health Related Quality Of Life); Waist-to-height Ratio	Johns Hopkins Bloomberg School of Public Health	2026-10-01	PHASE3
NCT07697196	NOT_YET_RECRUITING	HPV Infection; HPV Integration; HPV Disease; HPV Genital Infection; HPV Persistence; Low Grade Squamous Intraepithelial Lesions of the Cervix	IRCCS San Raffaele	2026-07
NCT07680595	RECRUITING	Carotid Atherosclerotic Plaque	Ruijin Hospital	2026-03-01	PHASE1; PHASE2

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Folic acid Vitamin chemical

Chemical Structure

Molecular Weight: 441.40