Folic acid

For research use only.

Catalog No.S4605 Synonyms: Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate

Folic acid Chemical Structure

Molecular Weight(MW): 441.40

Folic acid, a B vitamin, plays an important role in cell division and in the synthesis of amino acids and nucleic acids like DNA.

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Biological Activity

Description Folic acid, a B vitamin, plays an important role in cell division and in the synthesis of amino acids and nucleic acids like DNA.
In vitro

Folic acid treatment induces dose-related increases in BRCA1 mRNA expression in HepG2, Huh-7D12, Hs578T, and JURKAT and in BRCA2 in HepG2, Hs578T, MCF7, and MDA-MB-157 cells. FA does not affect the corresponding normal cells or on any of the ovarian cell lines. Folic acid induces increased BRCA1 protein expression in Hs578T, but not HepG2 cells, whereas BRCA2 protein levels are undetectable. FA treatment does not alter DNA repair in liver-derived cells, whereas there are transient effects on breast-derived cells. There is no effect of FA treatment on BRCA1 or BRCA2 DNA methylation, although there is some variation in the methylation of specific CpG loci between some cell lines[1].

Protocol

Cell Research:

[1]

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  • Cell lines: HepG2, Huh-7D12, Hs578T, JURKAT, Hs578T, MCF7, and MDA-MB-157 cells
  • Concentrations: 0, 25, 50, 75, or 100 nmol/L
  • Incubation Time: 72 h
  • Method:

    To determine the effect of FA supplementation on BRCA1 and BRCA2 mRNA expression, all cell lines were treated with 0, 25, 50, 75, or 100 nmol/L FA for 72 hours before harvesting in TRI Reagent according to the manufacturer’s instructions.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: ICR strain mice
  • Dosages: 3 mg/kg
  • Administration: by gastric intubation
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL warmed (4.53 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 441.40
Formula

C19H19N7O6

CAS No. 59-30-3
Storage powder
in solvent
Synonyms Folacin, Vitamin B9, Vitamin M, Pteroylglutamic acid, Folate
Smiles NC1=NC(=O)C2=C(N1)N=CC(=N2)CNC3=CC=C(C=C3)C(=O)NC(CCC(O)=O)C(O)=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04310059 Not yet recruiting Dietary Supplement: Folic acid|Dietary Supplement: Materna Thalassemia|Folic Acid Deficiency Anemia The University of Hong Kong|Queen Mary Hospital Hong Kong April 1 2020 Not Applicable
NCT04300556 Not yet recruiting Drug: MORAb-202 Solid Tumor Eisai Inc. February 28 2020 Phase 1|Phase 2
NCT03853304 Not yet recruiting Other: + Folic acid|Other: + Vitamin B12|Other: DFS Anemia|Folate Deficiency|Vitamin B 12 Deficiency Cornell University|Centers for Disease Control and Prevention|Arogyavaram Medical Centre January 1 2020 Not Applicable
NCT04216446 Not yet recruiting Behavioral: Mobile Health Intervention Pregnancy Aga Khan University January 2020 Not Applicable
NCT04209634 Recruiting Drug: Pozelimab CD55-deficient Protein-losing Enteropathy|CHAPLE Regeneron Pharmaceuticals January 27 2020 Phase 2|Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID