FCCP

Catalog No.S8276 Synonyms: Trifluoromethoxy carbonylcyanide phenylhydrazone

FCCP Chemical Structure

Molecular Weight(MW): 254.17

FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

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Cited by 14 Publications

5 Customer Reviews

  • The changes in 10-N-nonylacridineorange (NAO) fluorescence indicated the cardiolipin (CL) oxidation. In normal cells, NAO could interact with nonoxidized cardiolipin and produces a characteristic green fluorescence. However, after cardiolipin is oxidized, NAO cannot bind to it. F-H, The changes in MDA, GSH, and SOD levels. I and J, The cytoplasmic [Ca2+] map via confocal microscopy by Fluo-2. Fluorescence intensity of Fluo-2 was measured by excitation wavelengths of 340 nm and emission wavelengths of 500 nm. Data (F/F0) were obtained by dividing fluorescence intensity (F) by (F0) at the resting level (t = 0), which was normalized to normal groups. Data are shown as means ± SEM. *P < .05 vs normal group; #P < .05 vs PA group, @P < .05 vs PA + siRNA-NR4A1 or PA + melatonin groups

    J Pineal Res, 2018, 64:e12450. FCCP purchased from Selleck.

  • The change in the membrane potential (ΔΨm) via JC-1 staining

    Redox Biology, 2018, 14:576-587. FCCP purchased from Selleck.

  • The mitophagy activity was examined via the overlap of mitochondria and lysosome. Compared to the control cells, Yap-deleted HepG2 cells had more lysosome containing mitochondria. 3-MA was the inhibitor of mitophagy and 3-MA could alleviate the promotive effect of Yap deficiency on mitophagy. FCCP was the inducer of mitophagy, which was used as the positive control group.

    Redox Biology, 2018, 14:59-71. FCCP purchased from Selleck.

  • The mitochondrial fission was observed via immunofluorescence. More shorter mitochondria appeared in response to LPS treatment. However, melatonin treatment could reverse the mitochondrial length. Mdivi1 and FCCP were the inhibitor and activator of mitochondrial fission, respectively.

    Cell Stress and Chaperones, 2018, 23(2):281-293. FCCP purchased from Selleck.

  • The alteration of mitochondrial respiratory complex via western blotting assay.

    Cell Stress Chaperones, 2018, doi: 10.1007/s12192-018-0886-1. FCCP purchased from Selleck.

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Biological Activity

Description FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
T47D cells MnT6SpVv[3Srb36gZZN{[Xl? NWPodFhKUW6qaXLpeIlwdiCxZjCxMEAyOC2yaHXuZY51cHKxbHnu[U1qdmS3Y3XkJGhKTjFiYXP0bZZifGmxbjDpckBpfW2jbjDUOFdFKGOnbHzzJIJ6KEiURUOtWGsudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxRTBwM{Gg{txO MUSyNFkzQTJ4MR?=
MDA-MB-231 cells M2KwUWN6fG:2b4jpZ4l1gSCjc4PhfS=> MVWxJJVO M3q0[mN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36veoli[mmuaYT5JIF1KDFidV2= NWfVPYdTOjN{NEW2OVA>
Hep3B cells NG\u[XRHfW6ldHnvckBie3OjeR?= Ml7MNVAh|ryP M13PNXN1cW23bHH0bY9vKG:oIIP0ZZRmKDRiY3XscJVt[XJicnXzdIlz[XSrb36gbY4hcHWvYX6gTIVxO0JiY3XscJMh[XRiMUCgeW0hcW5icILld4Vv[2Vib3[gc4xq\2:veXPpci=> M3iwNVIzQTN6MEmz
TA3/Ha cells MVvGeY5kfGmxbjDhd5NigQ>? M3rmSmlv\HWldHnvckBw\iCQQVSoVElJKG:6aXTheIlwdiCrbjDtc5V{\SCWQUOvTIEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKG:oIF7BSEhRMUhxTlHELHAqMyC{YYTpc{BifCB4IIXNJIJ6KHOyZXP0do9ndHWxcn;t[ZRmeiCjbnHsfZNqew>? NWrTZVZMOjR3Nki2NVQ>
SH-SY5Y cells MoLjSpVv[3Srb36gZZN{[Xl? NFz0cogyOCEQvF2= M4rxPVExKG2rboO= NHS3eJRKdmS3Y4Tpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhdG:|czDpckBpfW2jbjDTTE1UYTW\IHPlcIx{KGG2IEGwJJVOKGmwY4XiZZRm\CCob4KgNVAhdWmwczDpckBxemW|ZX7j[UBw\iByLkKgeW0hS3ODIHL5JHROWkViZInlJIJie2WmIHHzd4F6 M{XmWVI2OjZ3MEK0

... Click to View More Cell Line Experimental Data

In vivo FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].

Protocol

Cell Research:

[1]

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  • Cell lines: PC12 cells
  • Concentrations: 30 μM
  • Incubation Time: 30 min, 1h, 2h
  • Method:

    Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (196.71 mM)
Ethanol 50 mg/mL (196.71 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 254.17
Formula

C10H5F3N4O

CAS No. 370-86-5
Storage powder
in solvent
Synonyms Trifluoromethoxy carbonylcyanide phenylhydrazone

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID