FCCP

For research use only.

Catalog No.S8276 Synonyms: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

27 publications

FCCP Chemical Structure

CAS No. 370-86-5

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

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Selleck's FCCP has been cited by 27 publications

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Biological Activity

Description FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
Targets
OXPHOS [2]
()
ATP synthase [2]
()
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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DLD1 MUXGeY5kfGmxbjDhd5NigQ>? MX2xJJVO MUXVcoNwfXCuaX7nJI9nKG2rdH;jbI9v\HKrYXygc5hq\GG2aY\lJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDEUGQyKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIH;4fYdmdiClb37zeY1xfGmxbjDyZZRmKGG2IEGgeW0hcW5icILld4Vv[2Vib3[gc4xq\2:veXPpckBCKGK7IIPlZYhwenOnIGjG[Vk3KGGwYXz5d4VzKGKjc3XkJIF{e2G7 MmjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF5N{S2O|IoRjNzN{e0OlczRC:jPh?=
HEK293 Mmj3SpVv[3Srb36gZZN{[Xl? M3n2OlEhfG9iMzD1US=> M2PzUWlvcGmkaYTpc44hd2ZiTHnDcE1i[3SrdnH0[YQhX262IIPp[45idGmwZzDpckBJTUt{OUOgZ4VtdHNiYYSgNUB1dyB|IIXNJIJ6KFSRUF\sZZNpKHKncH;yeIVzKGenbnWgZZN{[Xl? NYfPdoR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3O|Q3PzJpPkOxO|c1Pjd{PD;hQi=>
KOPN8 NVrCPGRzTnWwY4Tpc44h[XO|YYm= MnTDNVAhfU1? NF72e4YxNjNiaILz NVfSTllMUW6mdXP0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGyxc4OgbY4hcHWvYX6gT29RVjhiY3XscJMh[XRiMUCgeW0h[W[2ZYKgNE4{KGi{czDifUBVVVKPIIP0ZYlvcW6pIHLhd4VlKG[ub4egZ5l1d22ndILpZ{BidmGueYPpdy=> M1jxN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMEi0NFI5Lz5|MUC4OFAzQDxxYU6=
HepG2 NVjoTmZRTnWwY4Tpc44h[XO|YYm= MYrMeYNq\mW{YYPlM4x2[2moZYLpck1mgHC{ZYPzbY5oKGGwdHnmc4xifGVvcnXzbZN1[W62IIDhdoF{cXSnczD3[ZJmKHW|ZXSgeI8hcW6oZXP0JIEh[3WudIXy[UBw\iCKZYDHNkBk\WyuczD0bIF1KHencnWgdJJmNWmwY4XiZZRm\CC5aYToJINwdXCxdX7kd{4hUW6oZXP0[YQhcGWyYYTvZ5l1\XNiZX3peEBtcWeqdDDkeYUhfG9idHjlJIx2[2moZYLhd4UhemWjY4Tpc44vKEG|c3H5JJJme3WudIOgZZJmKHC{ZYPlcpRm\CCjczD0bIUheGW{Y3XueEAtKEmFNUC9NE4zPDYQvF2= NYqzUYlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDV5NUC0M{c,S2iHTVLMQE9iRg>?

... Click to View More Cell Line Experimental Data

In vivo FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].

Protocol

Cell Research:

[1]

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  • Cell lines: PC12 cells
  • Concentrations: 30 μM
  • Incubation Time: 30 min, 1h, 2h
  • Method:

    Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (196.71 mM)
Water Insoluble
Ethanol '50 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 254.17
Formula

C10H5F3N4O

CAS No. 370-86-5
Storage powder
in solvent
Synonyms Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone
Smiles C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID