FCCP

For research use only.

Catalog No.S8276 Synonyms: Trifluoromethoxy carbonylcyanide phenylhydrazone

21 publications

FCCP Chemical Structure

Molecular Weight(MW): 254.17

FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

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Selleck's FCCP has been cited by 21 publications

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Biological Activity

Description FCCP is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
T47D cells NH3UV4JHfW6ldHnvckBie3OjeR?= Mn7WTY5pcWKrdHnvckBw\iBzLDCxNE1xcGWwYX70bJJwdGmwZT3pcoR2[2WmIFjJSlEh[WO2aY\heIlwdiCrbjDoeY1idiCWNEfEJINmdGy|IHL5JGhTTTNvVFutcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVAvOzFizszN MX[yNFkzQTJ4MR?=
MDA-MB-231 cells Mni0R5l1d3SxeHnjbZR6KGG|c3H5 NV\zflZvOSC3TR?= NYLWXFhiS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvck93cWGkaXzpeJkh[XRiMTD1US=> M3rXc|I{OjR3NkWw
Hep3B cells NE\0TGtHfW6ldHnvckBie3OjeR?= NW\JUmxxOTBizszN MX7TeIlufWyjdHnvckBw\iC|dHH0[UA1KGOnbHz1cIFzKHKnc4DpdoF1cW:wIHnuJIh2dWGwIFjldFNDKGOnbHzzJIF1KDFyIIXNJIlvKHC{ZYPlcoNmKG:oIH;sbYdwdXmlaX6= NUHjU|ZCOjJ7M{iwPVM>
TA3/Ha cells Ml36SpVv[3Srb36gZZN{[Xl? NF\l[I1KdmS3Y4Tpc44hd2ZiTlHELHAqUCCxeHnkZZRqd25iaX6gcY92e2ViVFGzM2hiKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCxZjDORWQpWCmKL17BSEhRMSticnH0bY8h[XRiNjD1UUBjgSC|cHXjeJJw\my3b4LvcYV1\XJiYX7hcJl{cXN? NYL2VopvOjR3Nki2NVQ>
SH-SY5Y cells MljqSpVv[3Srb36gZZN{[Xl? M2T2O|ExKM7:TR?= NFX5V|MyOCCvaX7z NYfkU3dMUW6mdXP0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGyxc4OgbY4hcHWvYX6gV2guW1l3WTDj[YxteyCjdDCxNEB2VSCrbnP1ZoF1\WRiZn;yJFExKG2rboOgbY4heHKnc3XuZ4Uhd2ZiMD6yJJVOKEO|QTDifUBVVVKHIHT5[UBj[XOnZDDhd5NigQ>? NELCcVMzPTJ4NUCyOC=>

... Click to View More Cell Line Experimental Data

In vivo FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].

Protocol

Cell Research:

[1]

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  • Cell lines: PC12 cells
  • Concentrations: 30 μM
  • Incubation Time: 30 min, 1h, 2h
  • Method:

    Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (196.71 mM)
Ethanol 50 mg/mL (196.71 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 254.17
Formula

C10H5F3N4O

CAS No. 370-86-5
Storage powder
in solvent
Synonyms Trifluoromethoxy carbonylcyanide phenylhydrazone
Smiles FC(F)(F)OC1=CC=C(NN=C(C#N)C#N)C=C1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID