Name: FCCP
Brand: Selleck Chemicals
SKU: S8276
Availability: InStock
Rating: 5 (59 reviews)

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Quality Control

Batch: Purity: 99.99%

99.99

Related Targets	Bcl-2 Caspase K-Ras PD-1/PD-L1 Ferroptosis p53 Apoptosis related Synthetic Lethality STAT TNF-alpha
Other OXPHOS Products	IACS-010759 (IACS-10759) ME-344 Gboxin VLX600 S-Gboxin 4-Methyl-2-oxovaleric acid NDUFS2 Antibody [N8J1] MS-L6 Mito-LND (Mito-Lonidamine) DX3-213B

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
T47D	Function assay			Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM	20929261
T47D	Function assay			Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM	20929261
T47D	Function assay	1 to 10 uM		Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA	20929261
T47D	Function assay	0.3 uM	15 mins	Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay	20929261
MDA-MB-231	Cytotoxicity assay	1 uM		Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM	23245650
MDA-MB-231	Cytotoxicity assay	0.1 to 3 uM		Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor	23245650
MDA-MB-231	Function assay	0.3 uM		Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM	23245650
T47D	Function assay	0.1 to 3 uM		Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM	22938093
T47D	Function assay	10 to 30 uM		Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin	22938093
T47D	Function assay	0.3 to 1 uM		Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin	22938093
Hep3B	Function assay	10 uM		Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin	22938093
Hep3B	Function assay	1 uM		Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin	22938093
T47D	Function assay	3 uM	15 to 20 mins	Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay	22938093
TA3/Ha	Function assay	6 uM		Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis	24568614
SH-SY5Y	Function assay	10 uM	5 mins	Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay	25265024
SH-SY5Y	Function assay	10 uM	10 mins	Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay	25265024
T47D	Function assay	0.3 uM	3 to 12 mins	Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay	26637046
T47D	Function assay	0.3 uM	30 mins	Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy	26637046
T47D	Function assay	0.3 uM		Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate	26637046
HCT116	Function assay	2 uM	30 mins	Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method	28233680
HCT116	Function assay	2 uM	30 mins	Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method	28233680
DLD1	Function assay	1 uM		Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay	31774672
DLD1	Function assay	1 uM		Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay	31774672
LS174T	Function assay	1 uM		Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay	31774672
LS174T	Function assay	1 uM		Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay	31774672
DLD1	Function assay	1 uM		Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay	31774672
DLD1	Function assay	1 uM		Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay	31774672
HEK293	Function assay	1 to 3 uM		Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay	31774672
KOPN8	Function assay	10 uM	0.3 hrs	Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis	31084028
HepG2	Function assay			Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM	ChEMBL
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 6 mg/mL (23.6 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.300mg/ml (5.11mM)	Taking the 1 mL working solution as an example, add 50 μL of 26 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.300mg/ml (1.18mM)	Taking the 1 mL working solution as an example, add 50 μL of 6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	254.17	Formula	C₁₀H₅F₃N₄O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	370-86-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone			Smiles	C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F

Mechanism of Action

Targets/IC₅₀/Ki	OXPHOS ATP synthase
In vitro	FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity. This compound also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction.
In vivo	FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although this compound-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype.
References	[1] https://pubmed.ncbi.nlm.nih.gov/11248255/ [2] https://pubmed.ncbi.nlm.nih.gov/22686625/ [3] https://pubmed.ncbi.nlm.nih.gov/25715796/ [4] https://pubmed.ncbi.nlm.nih.gov/35163244/

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FCCP OXPHOS Uncoupler

Chemical Structure

Molecular Weight: 254.17