FCCP

Catalog No.S8276 Synonyms: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

For research use only.

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

FCCP Chemical Structure

CAS No. 370-86-5

Selleck's FCCP has been cited by 36 publications

Purity & Quality Control

Choose Selective OXPHOS Inhibitors

Biological Activity

Description FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
Targets
OXPHOS [2]
()
ATP synthase [2]
()
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1]. FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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MDA-MB-231 MWTDfZRwfG:6aXPpeJkh[XO|YYm= MoTSNUB2VQ>? NYrZNJF5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvck93cWGkaXzpeJkh[XRiMTD1US=> NF7FOFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K0OVY2OCd-MkOyOFU3PTB:L3G+
MDA-MB-231 M2LZcGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXSwMlEhfG9iMzD1US=> NUnsTXJrS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxicILvcIln\XKjdHnvck93cWGkaXzpeJkh[XRiMD6xJJRwKDNidV2gbY4heHKnc3XuZ4Uhd2ZiMD6xJJVOKHKxdHXuc45mKG2rdH;jbI9v\HKrYXyg[Yxm[3S{b36geJJidnOyb4L0JIlvcGmkaYTvdi=> NGTFOZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K0OVY2OCd-MkOyOFU3PTB:L3G+
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T47D NVna[pR[TnWwY4Tpc44h[XO|YYm= NGfyeGE{KHWP MXuxOUB1dyB{MDDtbY5{ M4rXe2Rm[3KnYYPlJIlvKG2rdH;jbI9v\HKrYXygcYVu[nKjbnWgdI91\W62aXHsJIlvKGi3bXHuJHQ1P0RiY3XscJMh[XRiMzD1UUBi\nSncjCxOUB1dyB{MDDtbY5{KGK7IGTNVm0h[XO|YYm= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|OEC5N{c,OjJ7M{iwPVM9N2F-
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SH-SY5Y M{XrXWZ2dmO2aX;uJIF{e2G7 NFT3d4YyOCC3TR?= NGTLPFIyOCCvaX7z NVryPIdCUW6mdXP0bY9vKG:oIH3peI9kcG:wZILpZYwhdWWvYoLhcoUheG:2ZX70bYFtKGyxc4OgbY4hcHWvYX6gV2guW1l3WTDj[YxteyCjdDCxNEB2VSCrbnP1ZoF1\WRiZn;yJFExKG2rboOgbY4heHKnc3XuZ4Uhd2ZiMD6yJJVOKEO|QTDifUBVVVKHIHT5[UBj[XOnZDDhd5NigQ>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ4NUCyOEc,OjV{NkWwNlQ9N2F-
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DLD1 NHO0VVJHfW6ldHnvckBie3OjeR?= Moe4NUB2VQ>? NX;XSppyXW6lb4XwcIlv\yCxZjDtbZRw[2ixbnTybYFtKG:6aXTheIl3\SCyaH;zdIhwenmuYYTpc44hcW5iaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBwgHmpZX6gZ49ve3WvcITpc44hemG2ZTDheEAyKHWPIHnuJJBz\XOnbnPlJI9nKG:uaXfvcZlkcW5iQTDifUB{\WGqb4Lz[UBZTmV7NjDhcoFtgXOncjDiZZNm\CCjc4PhfS=> NGnkb4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUe3OFY4Oid-M{G3O|Q3PzJ:L3G+
HEK293 NVjaR5lXTnWwY4Tpc44h[XO|YYm= NELN[3kyKHSxIEOgeW0> NXn3WJVNUW6qaXLpeIlwdiCxZjDMbWNtNWGldHn2ZZRm\CCZboSgd4lodmGuaX7nJIlvKEiHS{K5N{Bk\WyuczDheEAyKHSxIEOgeW0h[nliVF;QSoxie2hicnXwc5J1\XJiZ3Xu[UBie3OjeR?= NVzTcW1GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3O|Q3PzJpPkOxO|c1Pjd{PD;hQi=>
KOPN8 M4XJbGZ2dmO2aX;uJIF{e2G7 MWWxNEB2VQ>? NF20NGYxNjNiaILz NEj0T3ZKdmS3Y4Tpc44hd2ZibXn0c4Npd26mcnnhcEBu\W2kcnHu[UBxd3SnboTpZYwhdG:|czDpckBpfW2jbjDLU3BPQCClZXzsd{BifCBzMDD1UUBi\nSncjCwMlMhcHK|IHL5JHROWk1ic4ThbY5qdmdiYnHz[YQh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz NImzSmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUC4OFAzQCd-M{GwPFQxOjh:L3G+
HepG2 MV;GeY5kfGmxbjDhd5NigQ>? MlzaUJVkcW[ncnHz[U9tfWOrZnXybY4u\XiycnXzd4lv\yCjboTp[o9t[XSnLYLld4l{fGGwdDDwZZJie2m2ZYOge4Vz\SC3c3XkJJRwKGmwZnXjeEBiKGO3bIT1doUhd2ZiSHXwS|Ih[2WubIOgeIhifCC5ZYLlJJBz\S2rbnP1ZoF1\WRid3n0bEBkd22yb4Xu[JMvKEmwZnXjeIVlKGincHH0c4N6fGW|IHXtbZQhdGmpaISg[JVmKHSxIITo[UBtfWOrZnXyZZNmKHKnYXP0bY9vNiCDc4PhfUBz\XO3bITzJIFz\SCycnXz[Y51\WRiYYOgeIhmKHCncnPlcpQhNCCLQ{WwQVAvOjR3zszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0OVc2ODRxJ{7DbGVOSkx:L3G+
In vivo FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: PC12 cells
  • Concentrations: 30 μM
  • Incubation Time: 30 min, 1h, 2h
  • Method:

    Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.

Solubility (25°C)

In vitro

DMSO 50 mg/mL
(196.71 mM)
Water Insoluble
Ethanol '50 mg/mL

Chemical Information

Molecular Weight 254.17
Formula

C10H5F3N4O

CAS No. 370-86-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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