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Gboxin OXPHOS inhibitor

Name: Gboxin
Brand: Selleck Chemicals
SKU: S8828
Availability: InStock
Rating: 5 (4 reviews)

Catalog No.S8828 Purity:99.76%

Gboxin is an inhibitor of oxidative phosphorylation in cancer cells. This compound inhibits the activity of F0F1 ATP synthase. It specifically inhibits the growth of primary mouse and human glioblastoma cells but not that of mouse embryonic fibroblasts or neonatal astrocytes.

Chemical Structure

Molecular Weight: 392.96

Purity & Quality Control

Batch: S882801 Ethanol]79 mg/mL]false]DMSO]39 mg/mL]false]Water]2 mg/mL]false Purity: 99.76%

99.76

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Mechanism of Action

Targets

oxidative phosphorylation ^[1]

F0F1 ATP synthase ^[1]

In vitro
In vitro	Gboxin rapidly and irreversibly compromises GBM oxygen consumption. Reliant on its positive charge, this compound associates with mitochondrial oxidative phosphorylation complexes in a proton gradient dependent manner and inhibits F0F1 ATP synthase activity. It targets unique features of mitochondrial pH in GBM and other cancer cells, independent of their genetic composition, and exerts its tumor cell specific toxicity in primary culture and in vivo^[1].
Cell Research	Cell lines	HTS, MEF and astrocyte cells
	Concentrations	0-15 μM
	Incubation Time	96 h
	Method	--
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	ATF4 / p-S6 / S6 pAMPK / AMPK / pACC-79 / ACC		30842654
	Growth inhibition assay	Cell viability		30842654

References

https://pubmed.ncbi.nlm.nih.gov/30842654/

Chemical Information

Molecular Weight	392.96	Formula	C₂₂H₃₃ClN₂O₂
CAS No.	2101315-36-8	SDF	--
Synonyms	N/A
Smiles	CCC1=[N+](C2=CC=CC=C2N1CC(=O)OC3CC(CCC3C(C)C)C)C.[Cl-]

Storage and Stability

Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

Ethanol : 79 mg/mL

DMSO : 39 mg/mL ( (99.24 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 2 mg/mL

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.950mg/ml (4.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 39 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.970mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 19.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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