Gboxin

Catalog No.S8828

For research use only.

Gboxin is an inhibitor of oxidative phosphorylation in cancer cells. Gboxin inhibits the activity of F0F1 ATP synthase. It specifically inhibits the growth of primary mouse and human glioblastoma cells but not that of mouse embryonic fibroblasts or neonatal astrocytes.

Gboxin Chemical Structure

CAS No. 2101315-36-8

Purity & Quality Control

Choose Selective OXPHOS Inhibitors

Biological Activity

Description Gboxin is an inhibitor of oxidative phosphorylation in cancer cells. Gboxin inhibits the activity of F0F1 ATP synthase. It specifically inhibits the growth of primary mouse and human glioblastoma cells but not that of mouse embryonic fibroblasts or neonatal astrocytes.
Targets
oxidative phosphorylation [1]
()
F0F1 ATP synthase [1]
()
In vitro

Gboxin rapidly and irreversibly compromises GBM oxygen consumption. Reliant on its positive charge, Gboxin associates with mitochondrial oxidative phosphorylation complexes in a proton gradient dependent manner and inhibits F0F1 ATP synthase activity. Gboxin targets unique features of mitochondrial pH in GBM and other cancer cells, independent of their genetic composition, and exerts its tumor cell specific toxicity in primary culture and in vivo[1].

Assay
Methods Test Index PMID
Western blot ATF4 / p-S6 / S6 ; pAMPK / AMPK / pACC-79 / ACC 30842654
Growth inhibition assay Cell viability 30842654

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HTS, MEF and astrocyte cells
  • Concentrations: 0-15 μM
  • Incubation Time: 96 h
  • Method:

    --

Solubility (25°C)

In vitro

Ethanol 79 mg/mL
(201.03 mM)
DMSO 39 mg/mL
(99.24 mM)
Water 2 mg/mL
(5.08 mM)

Chemical Information

Molecular Weight 392.96
Formula

C22H33ClN2O2

CAS No. 2101315-36-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1=[N+](C2=CC=CC=C2N1CC(=O)OC3CC(CCC3C(C)C)C)C.[Cl-]

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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