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Dibutyryl cAMP (Bucladesine) sodium PKA activator

Cat.No.S7858

Bucladesine sodium is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.

Chemical Structure

Molecular Weight: 491.37

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.96%

99.96

Related Targets	Adrenergic Receptor AChR 5-HT Receptor Histamine Receptor Dopamine Receptor Ras GPR GluR Prostaglandin Receptor CXCR Adenosine Receptor P2 Receptor CCR Angiotensin Receptor Hedgehog/Smoothened S1P Receptor Cannabinoid Receptor cAMP Glucagon Receptor SGLT Opioid Receptor Sigma Receptor PAR Endothelin Receptor LTR Neurokinin Receptor Guanylate Cyclase PKG LPA Receptor OX Receptor
Related Products	H-89 Dihydrochloride 8-Bromo-cAMP Sodium Salt PKI 14-22 amide,myristoylated Malantide CREBtide Phospho-PKA α/β/γ (catalytic subunit) (T197) Antibody [F5K1] H-89 PKI(5-24) PKA C-α Antibody [P7L4] Bucladesine calcium A-3 hydrochloride PKA RI-α Antibody [J14D6]

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
mouse RAW264.7 cells	Function assay		4 h	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA, IC50=28.9 μM	11000020
mouse S49 cells	Cytotoxicity assay	500 μM	20-49 h	Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition at 500 uM after 20 to 49 hrs by time-course study	221658
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	491.37	Formula	C₁₈H₂₃N₅NaO₈P	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	16980-89-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	DBcAMP sodium salt			Smiles	CCCC(=O)NC1=C2C(=NC=N1)N(C=N2)C3C(C4C(O3)COP(=O)(O4)[O-])OC(=O)CCC.[Na+]

Solubility

In vitro
Batch:

DMSO : 98 mg/mL (199.44 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 98 mg/mL

Ethanol : 25 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.900mg/ml (9.97mM)	Taking the 1 mL working solution as an example, add 50 μL of 98 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	PDE ^[5] PKA ^[1]
In vitro	Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. ^[1] In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. ^[2] Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. ^[3]
In vivo	In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. ^[4]
References	[1] https://pubmed.ncbi.nlm.nih.gov/11161593/ [2] https://pubmed.ncbi.nlm.nih.gov/11469895/ [3] https://pubmed.ncbi.nlm.nih.gov/16538385/ [4] https://pubmed.ncbi.nlm.nih.gov/26066527/ [5] https://pubmed.ncbi.nlm.nih.gov/22302126/ [6] https://pubmed.ncbi.nlm.nih.gov/9721736/

Applications

Methods	Biomarkers	Images	PMID
Immunofluorescence	α-SMAD 2/3		19858184

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Handling Instructions

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