Dibutyryl-cAMP (Bucladesine)

Catalog No.S7858 Synonyms: dbcAMP

For research use only.

Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.

Dibutyryl-cAMP (Bucladesine) Chemical Structure

CAS No. 16980-89-5

Selleck's Dibutyryl-cAMP (Bucladesine) has been cited by 21 publications

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Biological Activity

Description Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.
Targets
PDE [5] PKA [1]
In vitro

Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. [1] In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. [2] Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NUHBXZJvTnWwY4Tpc44h[XO|YYm= MYe0JIg> M1H2XmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxRWy2rbnT1Z4VlKFSQRnHsdIhiKHC{b3T1Z5Rqd25iYX\0[ZIhPCCqcoOgZpkhTUyLU1GsJGlEPTB;MkiuPUDPxE1? M2\R[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMECwNFIxLz5zMUCwNFAzODxxYU6=
mouse S49 cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= NYLWWXNsPTByIN88US=> M3rrTFIxNTR7IHi= NVzr[WFRS3m2b4TvfIlkcXS7IHHnZYlve3RiSFfQVnRie2VvZHXmbYNq\W62IH3veZNmKFN2OTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDheEA2ODBidV2gZYZ1\XJiMkCgeI8hPDliaILzJIJ6KHSrbXWtZ492enOnIIP0eYR6 MlHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzNkW4K|4zOjF4NUi8M4E,
Assay
Methods Test Index PMID
Immunofluorescence α-SMAD 2/3 19858184
In vivo

In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. [4]

Protocol (from reference)

Animal Research:

[4]

  • Animal Models: Mice
  • Dosages: 600 nM/mouse
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 491.37
Formula

C18H23N5NaO8P

CAS No. 16980-89-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCC(=O)NC1=C2C(=NC=N1)N(C=N2)C3C(C4C(O3)COP(=O)(O4)[O-])OC(=O)CCC.[Na+]

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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