Dibutyryl-cAMP (Bucladesine)

Synonyms: dbcAMP

Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.

Dibutyryl-cAMP (Bucladesine) Chemical Structure

Dibutyryl-cAMP (Bucladesine) Chemical Structure

CAS: 16980-89-5

Selleck's Dibutyryl-cAMP (Bucladesine) has been cited by 34 publications

Purity & Quality Control

Batch: Purity: 99.96%
99.96

Dibutyryl-cAMP (Bucladesine) Related Products

Choose Selective PKA Inhibitors

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells Function assay 4 h Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production after 4 hrs by ELISA, IC50=28.9 μM 11000020
mouse S49 cells Cytotoxicity assay 500 μM 20-49 h Cytotoxicity against HGPRTase-deficient mouse S49 cells assessed as growth inhibition at 500 uM after 20 to 49 hrs by time-course study 221658
Click to View More Cell Line Experimental Data

Biological Activity

Description Dibutyryl-cAMP (Bucladesine, dbcAMP) is a cell-permeable PKA activator by mimicing the action of endogenous cAMP. Dibutyryl-cAMP (Bucladesine) is also a phosphodiesterase (PDE) inhibitor.
Targets
PDE [5] PKA [1]
In vitro
In vitro

Dibutyryl-cAMP inhibits neuronal glucose uptake via PKA activation. [1]

In cultured rat hepatocytes, Dibutyryl-cAMP inhibits inducible nitric oxide synthase expression and NF-kappaB-binding activity. [2]

Dibutyryl-cAMP also suppress TNFalpha-induced hepatocyte apoptosis by inhibiting FADD up-regulation. [3]

Cell Research Cell lines PC12 cells
Concentrations 1 mM
Incubation Time 24 h
Method

Cells were incubated with 1 mM of db-cAMP.

Experimental Result Images Methods Biomarkers Images PMID
Immunofluorescence α-SMAD 2/3 19858184
In Vivo
In vivo

In a mouse model, bucladesine (600 nM/mouse, i.p.) reverses zinc chloride- and lead acetate-induced avoidance memory retention impairments. [4]

Animal Research Animal Models Mice
Dosages 600 nM/mouse
Administration i.p.

Chemical Information & Solubility

Molecular Weight 491.37 Formula

C18H23N5NaO8P

CAS No. 16980-89-5 SDF Download Dibutyryl-cAMP (Bucladesine) SDF
Smiles CCCC(=O)NC1=C2C(=NC=N1)N(C=N2)C3C(C4C(O3)COP(=O)(O4)[O-])OC(=O)CCC.[Na+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 98 mg/mL ( (199.44 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 98 mg/mL

Ethanol : 25 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Dibutyryl-cAMP (Bucladesine) | Dibutyryl-cAMP (Bucladesine) supplier | purchase Dibutyryl-cAMP (Bucladesine) | Dibutyryl-cAMP (Bucladesine) cost | Dibutyryl-cAMP (Bucladesine) manufacturer | order Dibutyryl-cAMP (Bucladesine) | Dibutyryl-cAMP (Bucladesine) distributor