Dexamethasone Sodium Phosphate
For research use only.
CAS No. 55203-24-2
Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects.
Selleck's Dexamethasone Sodium Phosphate has been cited by 9 publications
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Dexamethasone and largazole cooperate to suppress invasion and to restore E-cadherin localization to the cell peripher y. ( a) Phase contrast micrographs showing morphological changes in MDA-MB-231 cells induced by E-cadherin expression combined with 100 nM dexamethasone and 10 nM largazole treatments. Insets show the cells at higher magnification. (b ) Fluorescence (E-Cad-GFP) or immunofluorescence microscopy (g -catenin (g-Cat.)) of 231/E-Cad-GFP cells treated for 72 h with vehicle (Control), 100 n M dexamethasone, 10 nM largazole or 100 nM dexamethasone + 10 nM largazole (Dex. + Larg.). (c ) Invasion assays were per formed with the indicated cell lines treated for 72 h with or without 100 nM dexamethasone + 10 nM largazole using modified Boyden chambers impregnated with matrigel. The results are presented as the average number of cells that invaded through the membrane per field s.d. of five randomly chosen fields, and are representative of three independently per formed experiments.
Oncogene 2013 32, 1316-29. Dexamethasone Sodium Phosphate purchased from Selleck.
(d) BT549 cells were treated and analyzed by immunofluorescence microscopy as in Figure b. (e) BT549 cells were treated as described in Figure b and analyzed for invasion as in Figure 3c. (f) Quantitation of junctional E-cadherin staining of the indicated cell lines treated with DMSO vehicle or Dex.+ Larg. as described in Figure b. Results are presented as the mean of analyses of three different fields of cells for each sample±s.d. Statistical significance was assessed using Student’s t -test.
Oncogene 2013 32, 1316-29. Dexamethasone Sodium Phosphate purchased from Selleck.
Purity & Quality Control
Choose Selective IL Receptor Inhibitors
|Description||Dexamethasone is a potent synthetic member of the glucocorticoid class of steroid drugs, and an interleukin receptor modulator that has anti-inflammatory and immunosuppressant effects.|
|Features||Great selectivity for COX-2.|
Dexamethasone inhibits COX-2 mRNA expression induced by IL-1 in human articular chondrocytes.  Dexamethasone suppresses the cyclooxygenase-2 induction by tumor necrosis factor α (TNFα) with an IC50 of 1 nM in MC3T3-E1 cells. Dexamethasone binds to the glucocorticoid receptor and then to the glucocorticoid response element. Dexamethasone (10 μM) induces osteoblastic differentiation of rat bone marrow stromal cell cultures with elevated mRNA expression of alkaline phosphatase osteopontin, bone sialoprotein, and osteocalcin.  Dexamethasone (5 μM) treatment decreases proliferation of adult hippocampal neural progenitor cells and SRE-driven gene expression. 
|In vivo||Dexamethasone (2 mg/kg) reduces the number of the BrdU-labeled hepatocytes by 80% in male Fischer F344 rats. Dexamethasone (2 mg/kg) pretreatment suppresses the expression of both TNF and IL-6 after partial hepatectomy and significantly reduces the proliferative response of the hepatocytes in male Fischer F344 rats. Dexamethasone also severely diminishes the induction and expansion of oval cells induced by the 2-acetylaminofluorene/partial hepatectomy (AAF/PH) protocol but does not have any effect on the proliferation of the bile duct cells stimulated by bile duct ligation.  Dexamethasone (100 μg/kg) produces a significant decrease 59.2% of BrdU(+) hippocampal progenitor cells in Sprague–Dawley rats. Dexamethasone (100 μg/kg) decreases ERK activation in granule cell layer in Sprague–Dawley rats. |
-  Blanco FJ, et al. J Rheumatol, 1999, 26(6), 1366-1373.
-  Yamamoto K, et al. FEBS Lett, 2000, 465(2-3), 103-106.
-  Rickard DJ, et al. Dev Biol, 1994, 161(1), 218-228.
|In vitro||Water||103 mg/mL (199.45 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04345588||Not yet recruiting||Drug: Dexamethasone injection||Dexamethasone Efficacy as an Adjuvant in Supraclavicular Block||Assiut University||January 1 2021||Phase 2|
|NCT03660072||Not yet recruiting||Other: Non-Interventional||Multiple Myeloma||Bristol-Myers Squibb||January 31 2021||--|
|NCT04246333||Not yet recruiting||Other: Mode of Delivery of Feeds||BPD - Bronchopulmonary Dysplasia|VLBW - Very Low Birth Weight Infant|Feeding Disorder Neonatal|Feeding; Difficult Newborn|Premature Birth|Chronic Lung Disease of Prematurity||Johns Hopkins All Children''s Hospital||September 1 2020||Not Applicable|
|NCT04529512||Not yet recruiting||Drug: DEXTENZA®||Ocular Disease Requiring Surgery||Nathan Steinle|Ocular Therapeutix Inc.|California Retina Consultants||August 2020||Phase 4|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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