AP26113-analog (ALK-IN-1)

For research use only.

Catalog No.S7000

4 publications

AP26113-analog (ALK-IN-1) Chemical Structure

CAS No. 1197958-12-5

AP26113-analog (ALK-IN-1) is an analog of AP26113 which is a potent and selective ALK inhibitor. It is also an inhibitor of EGFR.

Selleck's AP26113-analog (ALK-IN-1) has been cited by 4 publications

1 Customer Review

  • Several ALK inhibitors effectively inhibit the growth of CD74–ROS1–addicted Ba/F3 cells. A, Ba/F3 cells expressing CD74–ROS1 (clone #6) were seeded in 96-well plates and treated with the indicated concentration of crizotinib, ceritinib, AP26113, ASP3026, or alectinib for 72 hours. Cell viability was analyzed using the CellTiter-Glo Assay. B, IC50 values (nmol/L) of Ba/F3 cell lines expressing CD74–ROS1 (clone #6) against various ALK inhibitors are shown. Average IC50 values against crizotinib, ceritinib, or AP26113 were calculated from the three independent experiments. IC50 values against ASP3026 and alectinib were calculated from the single experiment. C, inhibition of phospho-ROS1 by various ALK inhibitors in Ba/F3 models. CD74–ROS1–expressing Ba/F3 cells were exposed to increasing concentrations of crizotinib, ceritinib, AP26113, ASP3026, or alectinib for 3 hours. Cell lysates were immunoblotted to detect the indicated proteins.

    Clin Cancer Res,2015, 21(1):166-74.. AP26113-analog (ALK-IN-1) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description AP26113-analog (ALK-IN-1) is an analog of AP26113 which is a potent and selective ALK inhibitor. It is also an inhibitor of EGFR.
ALK [1]
(Cell-free assay)
EGFR(C797S/del19) [2]
(Cell-free assay)
IGF1R [1]
(Cell-free assay)
EGFR(del19) [1]
(Cell-free assay)
EGFR(del19) [2]
(Cell-free assay)
0.07 nM 28.4 nM 32 nM 36.8 nM 36.8 nM
In vitro

Brigatinib and AP26113-analog have similar potency against the triple-mutant EGFR with IC50 values of <100 nM. AP26113-analog suppresses phosphorylation of EGFR and its downstream signalling pathway in cells expressing all types of EGFR mutations[2].


Cell Research:


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  • Cell lines: Ba/F3 cells
  • Concentrations: 0.3 nM to 10 μM
  • Incubation Time: 72 h
  • Method:

    Three-day cell viability assays are carried out by plating 2,000, 1,500 and 2,000 cells per well of Ba/F3, PC9 or MGH121, respectively, into black transparent-bottom 96-well plates. On the same day for Ba/F3 cells and the following day for PC9 and MGH121 cells, the cells are treated with each TKI across a 10-dose range from 0.3 nM to 10 μM. After 72 h of drug treatment, cell viability is measured using the CellTiter-Glo assay.

    (Only for Reference)

Solubility (25°C)

In vitro Ethanol 106 mg/mL (200.37 mM)
DMSO 45 mg/mL (85.06 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
NMP+PEG300 (10+90, v+v)
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 529.01


CAS No. 1197958-12-5
Storage powder
in solvent
Synonyms N/A
Smiles CN(C)C1CCN(CC1)C2=CC(=C(C=C2)NC3=NC=C(C(=N3)NC4=CC=CC=C4P(=O)(C)C)Cl)OC

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% DMSO % % Tween 80 % ddH2O

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID