Anisomycin

Catalog No.S7409 Synonyms: Flagecidin, Wuningmeisu C

For research use only.

Anisomycin (Flagecidin, Wuningmeisu C) is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, and also act as a JNK activator. Anisomycin upregulates autophagy and increases apoptosis.

Anisomycin Chemical Structure

CAS No. 22862-76-6

Selleck's Anisomycin has been cited by 56 publications

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Biological Activity

Description Anisomycin (Flagecidin, Wuningmeisu C) is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis, and also act as a JNK activator. Anisomycin upregulates autophagy and increases apoptosis.
Targets
JNK [1]
In vitro

Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. Anisomycin (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 Mo[5SpVv[3Srb36gZZN{[Xl? MYXJcohq[mm2b4L5JINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gdJJw\HWlZTDjfZRwfG:6aXPpeJkh[WejaX7zeEBJTUt{OUOgZ4VtdHNuIFnDOVA:OC5yMt88UU4> MkLRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZyMEWyNVMoRjF4MEC1NlE{RC:jPh?=
HeLa MULGeY5kfGmxbjDhd5NigQ>? NGXXd4UyOCC3TR?= M13qZWlvcGmkaYTpc44hd2ZidILhcpNt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzMDD1UUBjgSB|NWOtcYV1cGmxbnnu[UBu\XSjYn;sbYMhdGGkZXzpcoche3S3ZIm= NVPQeHllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxOlUyOzZpPkG1NVY2OTN4PD;hQi=>
HEK293 MkfDSpVv[3Srb36gZZN{[Xl? MnXXNVAxKHWP NGrLdIIyPSCvaX7z M1nRWmlv[3KnYYPlJI9nKEqQSzDwbI9{eGixconsZZRqd25iaX6gWVUxPDh6IITy[YF1\WRidX70doFve2[nY4Tl[EBJTUt{OUOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDF3IH3pcpM> NYPV[FN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe3NFI4PTBpPkG3O|AzPzVyPD;hQi=>
HEK293 NVqzcJNpTnWwY4Tpc44h[XO|YYm= NVvS[JJtPTBidV2= NGG4N5UyPSCvaX7z MkLjTY5kemWjc3Wgc4YhXTVyNEi4MYlv\HWlZXSgTm5MKHCqb4PwbI9zgWyjdHnvckBqdiC3boTyZY5{\mWldHXkJGhGUzJ7MzDj[YxteyCjdDC1NEB2VSCjZoTldkAyPSCvaX7z MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdyMke1NEc,OTd5MEK3OVA9N2F-
RAW264.7 NHnRPVZHfW6ldHnvckBie3OjeR?= MoTUOUB2VQ>? Mnz2N|AhdWmwcx?= M{Sy[WFkfGm4YYTpc44hd2ZicEO4UWFRUyCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCyaH;zdIhwenmuYYTpc44h[XRiVHjyNVgxN1S7ckG4NkBifCB3IIXNJIFnfGW{IEOwJI1qdnNiYomgW4V{fGW{bjDicI91fGmwZzDhcoFtgXOrcx?= Mn\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OUSyPFYoRjJ|Mkm0Nlg3RC:jPh?=
Sf9 NXXUTJVYTnWwY4Tpc44h[XO|YYm= NXrRTIE4OTBidV2= Ml;SOkB1dyB{NDDodi=> NX:yTnVbUW6lcnXhd4Uhd2ZiQWTQJIxmfmWuIHnuJHNxd2SxcITldoEh\nK3Z3nw[ZJl[SBqZnHscEBiem27d3;ycUkhW2Z7IHPlcIx{KGG2IEGwJJVOKGGodHXyJFYhfG9iMkSgbJIh[nlibIXtbY5me2OnboSgZ4VtdCC4aXHibYxqfHliYYPzZZk> NEPqSFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OjNzOUKvK|5EcEWPQly8M4E,
Sf9 NI\E[lVEgXSxdH;4bYNqfHliYYPzZZk> MoizNVAhfU1? MX63NkBpeg>? MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBUeG:mb4D0[ZJiKG[{dXfpdIVz\GFiKH\hcIwh[XKveYfvdo0qKFOoOTDj[YxteyCjdDCxNEB2VSCjZoTldkA4OiCqcjDifUB1enmyYX6gZox2\SCmeXWg[ZhkdHW|aX;uJJRme3R? NXvJfYdHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDJ|MUmyM{c,S2iHTVLMQE9iRg>?
VERO-E6 NEXCe|dHfW6ldHnvckBie3OjeR?= MXS0PEBpenN? M3q5PGRmfGW{bXnuZZRqd25ib3[gTWM2OCC4YXz1[ZMh\m:{IHnubIljcXSrb36gc4YhW0GUUz3Dc3YuOiCrbnT1Z4VlKGO7dH;0c5hq[2m2eTDv[kBXTVKRLVW2JINmdGy|IHHmeIVzKDR6IHjveZJ{KCCneIDvd5Vz\SC2bzCwMlAyKE2RSTDTRXJUKEOxVj2yJJZqenW|IHL5JIhq\2hiY3;ueIVvfCCrbXHnbY5oNCCLQ{WwQVAvODoQvF2u MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NlMyQTJxJ{7DbGVOSkx:L3G+
VERO-E6 MUXGeY5kfGmxbjDhd5NigQ>? MX[0PEBpenN? NHfYW4xVd3irY3n0fUBESzVyIHHnZYlve3RiVlXSU{1GPiClZXzsd{Bl\XSncn3pcoVlKGG2IES4JIhwfXK|IHL5JIhq\2hiY3;ueIVvfCCrbXHnbY5oKCi|YX3lJINwdmSrdHnvcpMh[XNiMm;MSXkhf2m2aH;1eEBmgHCxc4Xy[UB1dyByLkCxJG1QUSCVQWLTJGNwXi1{II\pdpV{MSxiQ1O1NF0xNjIQvF2u M{\mZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQzOzF7Mj:nQmNpTU2ETEyvZV4>
Vero E6 M1TYd2Z2dmO2aX;uJIF{e2G7 MWLDR|UxKGSndHXycYlv[XSrb36gZZQhVU:LIECuNFA1KHW|aX7nJGNmdGyWaYTldk0hT2yxIDjDWGcqKGG|c3H5MEBx\XKob4Lt[YQhOyCmYYnzJJBwe3RvaX7m[YN1cW:wIHnuJHZmem9iRU[gZ4VtdHNuIFPDOVA9OC5|Od88UU4> Mo\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFI{OTl{Lze+R4hGVUKOPD;hQi=>
Vero E6 MlW2SpVv[3Srb36gZZN{[Xl? M2DtdGNEPTBiZHX0[ZJucW6jdHnvckBifCCPT1mgNE4xOSC3c3nu[{BE\WyuVHn0[ZIuKEeubzCoR3RIMSCjc4PhfUwheGW{Zn;ycYVlKDNiZHH5d{Bxd3O2LXnu[oVkfGmxbjDpckBX\XKxIFW2JINmdGy|LDDDR|UxRDBwM{pOwG0v MlTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFI{OTl{Lze+R4hGVUKOPD;hQi=>
Assay
Methods Test Index PMID
Western blot p-ERK / ERK / p-JNK / JNK / p-p38 / ATF3 ; PP2A / PP2C ; p-Keratin 20 / Keratin 20 / p-Keratin 18 / Keratin 18 / p-Keratin 8 / Keratin 8 / p-MK2 / p-hHSPB1 / hHSPB1 17014422 22684030 20724476
Immunofluorescence p-Keratin 8 / p-Keratin 18 / p-Keratin 20 20724476
Growth inhibition assay Cell viability 22684030
In vivo Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]

Protocol (from reference)

Kinase Assay:[2]
  • JNK phosphorylation:

    500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (final concentration 1% v/v). Puromycin is added (final concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the fluorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.

Cell Research:[4]
  • Cell lines: Ehrlich ascites carcinoma (EAC) cells
  • Concentrations: 500 ng/mL
  • Incubation Time: 48 h
  • Method: For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 µL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.
Animal Research:[4]
  • Animal Models: Male BALB/c mice
  • Dosages: 5 mg/kg
  • Administration: peritumorally

Solubility (25°C)

In vitro

DMSO 41 mg/mL warmed
(154.54 mM)
Ethanol 17 mg/mL warmed
(64.07 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 265.3
Formula

C14H19NO4

CAS No. 22862-76-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)OC1C(CNC1CC2=CC=C(C=C2)OC)O

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