Catalog No.S7409 Synonyms: Flagecidin 

Anisomycin Chemical Structure

Molecular Weight(MW): 265.3

Anisomycin is an antibiotic, which inhibits protein synthesis, and also act as a JNK activator.

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2 Customer Reviews

  • (c) Immunostaining of Cx32, ALB, CPS1, CK18 and ECAD in hESC-Heps induced with SB or anisomycin.

    Sci Rep, 2016, 6:37388. Anisomycin purchased from Selleck.

    Effect of TMZ (100 μmol/l for U87 cells, 50 μmol/l for U251 cells), anisomycin (4 μmol/l), SB203580 (10 μmol/l), TMZ+SB203580 (10 μmol/l) treatment on thephosphorylation of p38 and AQP4 for 24 h in U87 cells and U251 cells, detected by Western blotting. (A) Protein expression of p-p38, p38 and AQP4 in U87 cells with differenttreatments. (B) The ration of p-p38/p38 in U87 cells. (C) The proportion of AQP4 in GAPDH in U87 cells. (D) Protein expression of p-p38, p38 and AQP4 in U251 cells withdifferent treatments. (E) The ration of p-p38/p38 in U251 cells. (F) The proportion of AQP4 in GAPDH in U251 cells. *P< 0.05 versus the control group

    J Cell Biochem, 2017, 118(12):4905-4913. Anisomycin purchased from Selleck.

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Biological Activity

Description Anisomycin is an antibiotic, which inhibits protein synthesis, and also act as a JNK activator.
JNK [1]
In vitro

Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin (0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin (4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and JNK, while inactivated ERK1/2. Anisomycin (4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MUDDfZRwfG:6aXRCpIF{e2G7 NWTs[4pkUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJJJmeXWrcnXkJJRwKHC{b3T1Z4Uh[3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBKSzVyPUCuNFIh|ryP MV:xOlAxPTJzMx?=
human HeLa cells M2jOXWZ2dmO2aX;uJIF{e2G7 MXOxNEDPxE1? M1LwU2lvcGmkaYTpc44hd2ZidILhcpNt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzMDD1UUBjgSB|NWOtcYV1cGmxbnnu[UBu\XSjYn;sbYMhdGGkZXzpcoche3S3ZIm= NVTSdYlsOTVzNkWxN|Y>
mouse RAW264.7 cells NIPKTlZHfW6ldHnvckBie3OjeR?= NHT3WFU2KM7:TR?= NVPQTHp5OzBibXnudy=> MWrBZ5RqfmG2aX;uJI9nKHB|OF3BVGshcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMheGixc4Doc5J6dGG2aX;uJIF1KFSqckG4NE9VgXJzOEKgZZQhPSC3TTDh[pRmeiB|MDDtbY5{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN? NGLPTpczOzJ7NEK4Oi=>

... Click to View More Cell Line Experimental Data

In vivo Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]


Kinase Assay:[2]
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JNK phosphorylation:

500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (final concentration 1% v/v). Puromycin is added (final concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the fluorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
Cell Research:[4]
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  • Cell lines: Ehrlich ascites carcinoma (EAC) cells
  • Concentrations: 500 ng/mL
  • Incubation Time: 48 h
  • Method: For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 µL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: Male BALB/c mice
  • Formulation: PBS
  • Dosages: 5 mg/kg
  • Administration: peritumorally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 41 mg/mL warmed (154.54 mM)
Ethanol 17 mg/mL warmed (64.07 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 265.3


CAS No. 22862-76-6
Storage powder
in solvent
Synonyms Flagecidin 

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID