Isotretinoin

Name: Isotretinoin
Brand: Selleck Chemicals
SKU: S1379
Rating: 5 (4 reviews)

For research use only.

Catalog No.S1379 Synonyms: 13-cis retinoic acid

4 publications

CAS No. 4759-48-2

Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.

Selleck's Isotretinoin has been cited by 4 publications

Cell Rep, 2020, 32(12):108171

PubMed: 32966799 ( click the link to review the publication )

Oncogene, 2020, 39(21):4212-4226

PubMed: 32251364 ( click the link to review the publication )

Cell Death Dis, 2020, 11(9):773

PubMed: 32943619 ( click the link to review the publication )

Sci Rep, 2018, 8(1):12960

PubMed: 30154523 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Hydroxylase Inhibitors

Biological Activity

Description

Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.

Targets

hydroxylase ^[1]

In vitro

Isotretinoin directly interferes with the development of cranial neural crest cells. ^[1] Isotretinoin selectively affects neural crest cells by decreasing their cell-substratum adhesion. ^[2]

In vivo

Isotretinoin (500 ng/mL) and its main metabolite in the human, 4-oxo-isotretinoin, induce malformations similar to those seen in vivo. ^[1] Isotretinoin impairs explicit memory in Stage 2, but retention tests one month after Isotretinoin exposure ended, indicated recovery from this explicit memory impairment and evidence of enhanced implicit memory in the 10 mg and 15 mg ISO rats. ^[3] Isotretinoin slows the recovery of rod signaling after exposure to an intense bleaching light, and that rhodopsin regeneration is markedly slowed. Isotretinoin is also found to protect rat photoreceptors from light-induced damage. ^[4] Isotretinoin blocks the formation of A2E biochemically and the accumulation of lipofuscin pigments by electron microscopy. Isotretinoin also blocks the slower, age-dependent accumulation of lipofuscin in wild-type mice. ^[5]

Protocol

References

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Solubility (25°C)

In vitro	DMSO	60 mg/mL (199.7 mM)
	Water	Insoluble
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Isotretinoin SDF

Molecular Weight	300.44
Formula	C₂₀H₂₈O₂
CAS No.	4759-48-2
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	13-cis retinoic acid
Smiles	CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

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Number of animals

Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)

% DMSO+ % + % Tween 80+ % ddH₂O

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Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2020-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03939364	Not yet recruiting	Drug: SBS-101\|Drug: Placebo	Leukoplakia Oral	Skyline Biosciences	January 2022	Phase 1
NCT04594759	Recruiting	Drug: Isotretinoin	Acne Vulgaris	Medical University of South Carolina	November 11 2020	Phase 1\|Phase 2
NCT04660916	Completed	Drug: Isotretinoin	Isotretinoin\|Nail Abnormality	Sinan Özçelik\|Balikesir University	October 22 2019	--
NCT04204304	Completed	Drug: Isotretinoin	Musculoskeletal Disease Other\|Isotretinoin Adverse Reaction\|Vitamin D Deficiency	Ankara Training and Research Hospital	March 15 2019	Not Applicable
NCT03323801	Completed	Drug: 13-cis retinoic acid	Male Infertility Azoospermia	University of Washington	July 25 2017	Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Hydroxylase Signaling Pathway Map

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Isotretinoin