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Isotretinoin

Name: Isotretinoin
Brand: Selleck Chemicals
SKU: S1379
Rating: 5 (6 reviews)

Catalog No.S1379 Synonyms: 13-cis retinoic acid

For research use only.

Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.

CAS No. 4759-48-2

Selleck's Isotretinoin has been cited by 6 Publications

Purity & Quality Control

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Hydroxylase Signaling Pathway Map

Biological Activity

Description

Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.

Targets

hydroxylase ^[1]

In vitro

Isotretinoin directly interferes with the development of cranial neural crest cells. ^[1] Isotretinoin selectively affects neural crest cells by decreasing their cell-substratum adhesion. ^[2]

In vivo

Isotretinoin (500 ng/mL) and its main metabolite in the human, 4-oxo-isotretinoin, induce malformations similar to those seen in vivo. ^[1] Isotretinoin impairs explicit memory in Stage 2, but retention tests one month after Isotretinoin exposure ended, indicated recovery from this explicit memory impairment and evidence of enhanced implicit memory in the 10 mg and 15 mg ISO rats. ^[3] Isotretinoin slows the recovery of rod signaling after exposure to an intense bleaching light, and that rhodopsin regeneration is markedly slowed. Isotretinoin is also found to protect rat photoreceptors from light-induced damage. ^[4] Isotretinoin blocks the formation of A2E biochemically and the accumulation of lipofuscin pigments by electron microscopy. Isotretinoin also blocks the slower, age-dependent accumulation of lipofuscin in wild-type mice. ^[5]

Protocol (from reference)

References

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Solubility (25°C)

In vitro

Chemical Information

Molecular Weight	300.44
Formula	C₂₀H₂₈O₂
CAS No.	4759-48-2
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03939364	Withdrawn	Drug: SBS-101\|Drug: Placebo	Leukoplakia Oral	Skyline Biosciences	January 2022	Phase 1
NCT04594759	Active not recruiting	Drug: Isotretinoin	Acne Vulgaris	Medical University of South Carolina	November 11 2020	Phase 1\|Phase 2
NCT04660916	Completed	Drug: Isotretinoin	Isotretinoin\|Nail Abnormality	Sinan Özçelik\|Balikesir University	October 22 2019	--
NCT04204304	Completed	Drug: Isotretinoin	Musculoskeletal Disease Other\|Isotretinoin Adverse Reaction\|Vitamin D Deficiency	Ankara Training and Research Hospital	March 15 2019	Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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