Isotretinoin
For research use only.
Catalog No.S1379 Synonyms: 13-cis retinoic acid
4 publications
CAS No. 4759-48-2
Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.
Selleck's Isotretinoin has been cited by 4 publications
Purity & Quality Control
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Biological Activity
| Description | Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more. | |
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| Targets |
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| In vitro |
Isotretinoin directly interferes with the development of cranial neural crest cells. [1] Isotretinoin selectively affects neural crest cells by decreasing their cell-substratum adhesion. [2] |
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| In vivo | Isotretinoin (500 ng/mL) and its main metabolite in the human, 4-oxo-isotretinoin, induce malformations similar to those seen in vivo. [1] Isotretinoin impairs explicit memory in Stage 2, but retention tests one month after Isotretinoin exposure ended, indicated recovery from this explicit memory impairment and evidence of enhanced implicit memory in the 10 mg and 15 mg ISO rats. [3] Isotretinoin slows the recovery of rod signaling after exposure to an intense bleaching light, and that rhodopsin regeneration is markedly slowed. Isotretinoin is also found to protect rat photoreceptors from light-induced damage. [4] Isotretinoin blocks the formation of A2E biochemically and the accumulation of lipofuscin pigments by electron microscopy. Isotretinoin also blocks the slower, age-dependent accumulation of lipofuscin in wild-type mice. [5] |
Protocol
Solubility (25°C)
| In vitro | DMSO | 60 mg/mL (199.7 mM) |
|---|---|---|
| Water | Insoluble | |
| Ethanol | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 300.44 |
|---|---|
| Formula | C20H28O2 |
| CAS No. | 4759-48-2 |
| Storage |
powder in solvent |
| Synonyms | 13-cis retinoic acid |
| Smiles | CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Bio Calculators
Molarity Calculator
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Dilution Calculator
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|---|
| NCT04594759 | Recruiting | Drug: Isotretinoin | Acne Vulgaris | Medical University of South Carolina | November 11 2020 | Phase 1|Phase 2 |
| NCT04660916 | Completed | Drug: Isotretinoin | Isotretinoin|Nail Abnormality | Sinan Özçelik|Balikesir University | October 22 2019 | -- |
| NCT04204304 | Completed | Drug: Isotretinoin | Musculoskeletal Disease Other|Isotretinoin Adverse Reaction|Vitamin D Deficiency | Ankara Training and Research Hospital | March 15 2019 | Not Applicable |
| NCT03323801 | Completed | Drug: 13-cis retinoic acid | Male Infertility Azoospermia | University of Washington | July 25 2017 | Phase 2 |
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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