For research use only.
Catalog No.S1379 Synonyms: 13-cis retinoic acid
CAS No. 4759-48-2
Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.
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|Description||Isotretinoin (13-cis retinoic acid) was developed to be used as a chemotherapy medication for the treatment of brain cancer, pancreatic cancer and more.|
|In vivo||Isotretinoin (500 ng/mL) and its main metabolite in the human, 4-oxo-isotretinoin, induce malformations similar to those seen in vivo.  Isotretinoin impairs explicit memory in Stage 2, but retention tests one month after Isotretinoin exposure ended, indicated recovery from this explicit memory impairment and evidence of enhanced implicit memory in the 10 mg and 15 mg ISO rats.  Isotretinoin slows the recovery of rod signaling after exposure to an intense bleaching light, and that rhodopsin regeneration is markedly slowed. Isotretinoin is also found to protect rat photoreceptors from light-induced damage.  Isotretinoin blocks the formation of A2E biochemically and the accumulation of lipofuscin pigments by electron microscopy. Isotretinoin also blocks the slower, age-dependent accumulation of lipofuscin in wild-type mice. |
-  Webster WS, et al. J Craniofac Genet Dev Biol, 1986, 6(3), 211-222.
-  Smith-Thomas L, et al. Dev Biol, 1987, 123(1), 276-281.
-  Dopheide MM, et al. Pharmacol Biochem Behav, 2008, 91(2), 243-251.
|In vitro||DMSO||60 mg/mL (199.7 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||13-cis retinoic acid|
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation ()|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04594759||Recruiting||Drug: Isotretinoin||Acne Vulgaris||Medical University of South Carolina||November 11 2020||Phase 1|Phase 2|
|NCT04660916||Completed||Drug: Isotretinoin||Isotretinoin|Nail Abnormality||Sinan Özçelik|Balikesir University||October 22 2019||--|
|NCT04204304||Completed||Drug: Isotretinoin||Musculoskeletal Disease Other|Isotretinoin Adverse Reaction|Vitamin D Deficiency||Ankara Training and Research Hospital||March 15 2019||Not Applicable|
|NCT03323801||Completed||Drug: 13-cis retinoic acid||Male Infertility Azoospermia||University of Washington||July 25 2017||Phase 2|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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